Origin IGS: aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taagaattatgtgatagtgaaatgagaactggctagcccagttctttttgcgtggataaataagtttttttgtttctgatctagataaaagatttctcatctaaggctcaaatatggtataataagggtt Mask Tandem Repeat Region ================================================ aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Find is-nt database================================================ Query_seq: SSA_1626:SSA_1627|SSA_1626:SSA_1627:DNA translocase ftsK, putative:hypothetical protein:<-<-:1629468..1629597 130 aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1626:SSA_1627|SSA_1626:SSA_1627:DNA translocase ftsK, putative:hypothetical protein:<-<-:1629468..1629597 130 aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1626:SSA_1627|SSA_1626:SSA_1627:DNA translocase ftsK, putative:hypothetical protein:<-<-:1629468..1629597 130 aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 31 End: 129 .............................. M R N L L S R S E T K K L I Y P R K K N W A S Q F S F H Y H I I L . aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 51 HP_score: -2.8 Tail_Score: -3.71378 Start: 84 End: 108 Full_Region: TATTTATCCACGCAA AAAGAACTGG GCTAG CCAGTTCTCA TTTCACTATCACATA ...................................................................................AAAGAACTGGGCTAGCCAGTTCTCA...................... TransTerm Strand: + Conf: 69 HP_score: -8.9 Tail_Score: -2.95942 Start: 87 End: 105 Full_Region: TTATCCACGCAAAAA GAACTGG GCTAG CCAGTTC TCATTTCACTATCAC ......................................................................................GAACTGGGCTAGCCAGTTC......................... Find igs database================================================ Query_seq: SSA_1626:SSA_1627|SSA_1626:SSA_1627:DNA translocase ftsK, putative:hypothetical protein:<-<-:1629468..1629597 130 aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Intra-Species Hit: Count: 1 Min: 1 Max: 130 Len: 130 Subject: NC_009009_SSA_1626_SSA_1627|DNA translocase ftsK, putative:hypothetical protein|NEGATIVE:NEGATIVE|[1629468,1629597]|130 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 130 Query Start:1 Query End:130 Subject Strand: POSITIVE Subject Start: 1 Subject End: 130 aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacnnnnnnncttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta aacccttattataccatatttgagccttagatgagaaatcttttatctagatcagaaacaaaaaaacttatttatccacgcaaaaagaactgggctagccagttctcatttcactatcacataattctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCCUUAUUAUACCAUAUUUGAGCCUUAGAUGAGAAAUCUUUUAUCUAGAUCAGAAACAAAAAAACUUAUUUAUCCACGCAAAAAGAACUGGGCUAGCCAGUUCUCAUUUCACUAUCACAUAAUUCUUA .......(((((......(((((...((((((((((....)))))))))).))))).............................((((((((.....))))))))..............)))))..... (-21.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAUUAUGUGAUAGUGAAAUGAGAACUGGCUAGCCCAGUUCUUUUUGCGUGGAUAAAUAAGUUUUUUUGUUUCUGAUCUAGAUAAAAGAUUUCUCAUCUAAGGCUCAAAUAUGGUAUAAUAAGGGUU .....((((((..(((.......(((((((((.....)))))))))...((.(((((....(((((((((....((((...)))).)))))))))...)))))..)).....)))..))))))....... (-23.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 896 120 71 211 162 NC_009009:SSA_1626|5end_DNA_translocase_ftsK,_putative_1629377..1629607_NEGATIVE 301 112 81 183 150 NC_009009:SSA_1771|5end_Conserved_DNA_methyltransferase_protein,_putative_1758799..1759029_NEGATIVE 288 106 75 227 189 NC_009009:SSA_1595|5end_Tellurite_resistance,_putative_1601814..1602044_NEGATIVE 269 107 75 131 100 NC_009009:SSA_0308|5end_hypothetical_protein_304385..304615_POSITIVE 263 118 77 176 138 NC_009009:SSA_1423|5end_Single-stranded_DNA-specific_exonuclease,_5'-3',_putative_1433783..1434013_NEGATIVE 263 102 80 154 129 NC_009009:SSA_0155|5end_hypothetical_protein_150961..151191_POSITIVE 260 129 85 167 129 NC_009009:SSA_1415|5end_Oxidoreductase,_putative_1426508..1426738_NEGATIVE 257 102 76 206 184 NC_009009:SSA_2125|5end_Small_primase-like_protein_(Toprim_domain),_putative_2130019..2130249_NEGATIVE 255 105 78 231 201 NC_009009:SSA_1795|5end_Guanosine_3',5'-bis-pyrophosphate_(ppGpp)_synthetase,_putative_1777671..1777901_NEGATIVE 254 54 11 60 18 NC_009009:SSA_0608|5end_Macrolide-efflux_protein,_putative_598184..598414_NEGATIVE 253 114 79 149 109 NC_009009:SSA_0547|5end_Acyl_carrier_protein_synthase,_putative_541991..542221_POSITIVE 249 116 75 101 60 NC_009009:SSA_0730|5end_Arsenical_resistance_operon_transcription_repressor_(ArsR),_putative_714818..715048_POSITIVE 247 115 79 214 173 NC_009009:SSA_1269|5end_hypothetical_protein_1293352..1293582_NEGATIVE 243 37 1 135 102 NC_009009:SSA_2372|5end_hypothetical_protein_2373807..2374037_POSITIVE 240 105 62 224 165 NC_009009:SSA_1080|5end_Transcriptional_repressor_of_the_fructose_operon,_DeoR_family,_putative_1097538..1097768_POSITIVE 239 118 79 183 138 NC_009009:SSA_1243|5end_Dihydroorotate_dehydrogenase_electron_transfer_subunit,_putative_1268204..1268434_NEGATIVE 238 106 66 192 151 NC_009009:SSA_1453|5end_hypothetical_protein_1460208..1460438_NEGATIVE 235 117 78 97 53 NC_009009:SSA_1735|5end_UreX,_putative_1721072..1721302_NEGATIVE 235 118 75 92 48 NC_009009:SSA_1070|5end_Ribosomal_large_subunit_pseudouridine_synthase_D,_putative_1088742..1088972_POSITIVE 235 119 74 48 5 NC_009009:SSA_0980|5end_3-isopropylmalate_dehydratase_large_subunit,_putative_984004..984234_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 332 108 71 148 118 NC_009009:SSA_2189|3end_hypothetical_protein_2190279..2190429_NEGATIVE 311 129 95 80 44 NC_009009:SSA_0373|3end_Dihydroorotate_dehydrogenase,_putative_363080..363230_POSITIVE 301 112 81 87 54 NC_009009:SSA_1772|3end_hypothetical_protein_1758895..1759045_NEGATIVE 269 107 75 79 48 NC_009009:SSA_0307|3end_Glutamine_synthetase_type_1,_putative_304413..304563_POSITIVE 263 118 77 70 32 NC_009009:SSA_1424|3end_Hydrolase,_putative_1433889..1434039_NEGATIVE 263 102 80 63 38 NC_009009:SSA_0154|3end_hypothetical_protein_150950..151100_POSITIVE 255 105 78 77 47 NC_009009:SSA_1796|3end_Transcription_elongation_factor_GreA,_putative_1777825..1777975_NEGATIVE 253 114 79 41 1 NC_009009:SSA_0546|3end_Phospho-2-dehydro-3-deoxyheptonate_aldolase_(DAHP_synthatase),_possibly_tyr-sensitive,_putative_541963..542113_POSITIVE 251 118 85 149 117 NC_009009:SSA_1416|3end_hypothetical_protein_1426520..1426670_NEGATIVE 247 115 79 90 49 NC_009009:SSA_1270|3end_Flavodoxin_1293476..1293626_NEGATIVE 241 120 75 99 50 NC_009009:SSA_0827|3end_Conserved_uncharacterized_cytosolic_protein,_putative_806952..807102_POSITIVE 238 106 66 145 104 NC_009009:SSA_1454|3end_hypothetical_protein_1460255..1460405_NEGATIVE 235 118 75 108 64 NC_009009:SSA_1069|3end_Lipoprotein_signal_peptidase,_putative_1088838..1088988_POSITIVE 235 119 74 46 3 NC_009009:SSA_0978|3end_hypothetical_protein_984082..984232_POSITIVE 235 101 67 131 90 NC_009009:SSA_0065|3end_Low_molecular_weight_phosphotyrosine_protein_phosphatase,_putative_67677..67827_POSITIVE 234 119 80 30 1 NC_009009:SSA_0660|3end_Cell_division_protein_DivIVA,_putative_644672..644822_POSITIVE 233 111 75 91 53 NC_009009:SSA_0599|3end_hypothetical_protein_589007..589157_POSITIVE 231 119 71 68 25 NC_009009:SSA_2380|3end_hypothetical_protein_2385665..2385815_NEGATIVE 231 50 1 135 80 NC_009009:SSA_1497|3end_DCMP_deaminase,_putative_1503068..1503218_NEGATIVE 231 119 76 96 51 NC_009009:SSA_0507|3end_hypothetical_protein_506442..506592_POSITIVE