Origin IGS: ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagacagtaggacagaacttgattttttaaaatttagcttggcccacttattttgaggttcaggtttaaaaggtctgatagacaatttaaattgtctcagccacaatattttttgaacataataaagctagactgaggcatttttgtctcagtcttttttattttggtaaaaaactttagaaatattttaggatttcttgagaaatatctgaagtttggaagctatactagaaagtgcaaggaggagagaaaagagctggaagcaacaggacagctgatagaaactttagtttttctttaagtgtgagtctagagcagatcacttttaaaagcagactgaatgaccttattctctcttcttgcggcaaatttttccagaccttcagttttctgttgacgaggaaaaaagaatatgttaaagtaa Mask Tandem Repeat Region ================================================ ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Find is-nt database================================================ Query_seq: SSA_1594:SSA_1595|SSA_1594:SSA_1595:Metalloendopeptidase, putative:Tellurite resistance, putative:<-<-:1600617..1601040 424 ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1594:SSA_1595|SSA_1594:SSA_1595:Metalloendopeptidase, putative:Tellurite resistance, putative:<-<-:1600617..1601040 424 ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1594:SSA_1595|SSA_1594:SSA_1595:Metalloendopeptidase, putative:Tellurite resistance, putative:<-<-:1600617..1601040 424 ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 11 End: 112 .......... M F F F P R Q Q K T E G L E K F A A R R E N K V I Q S A F K S D L L ........................................................................................................................................................................................................................................................................................................................ Protein_Len: 52 Strand: + Start: 39 End: 194 ...................................... M K V W K N L P Q E E R I R S F S L L L K V I C S R L T L K E K L K F L S A V L L L P A L F S P P C T F ...................................................................................................................................................................................................................................... Protein_Len: 36 Strand: + Start: 316 End: 423 ........................................................................................................................................................................................................................................................................................................................... M L W L R Q F K L S I R P F K P E P Q N K W A K L N F K K S S S V L L S . Protein_Len: 53 Strand: - Start: 127 End: 285 .............................................................................................................................. L F V L T E I L Q G T A E L E K R E E K C K R T Y S G F K L Y K E L F G L I N R F N K V L I F F V S V F M ........................................................................................................................................... Protein_Len: 60 Strand: - Start: 2 End: 190 . Y E K K R T L L F S F T Q F F K G C S S L I L D N L R S K F T I Q E L S V S L S F S F N R D A T R N S G A R K E G G Q M .......................................................................................................................................................................................................................................... ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 3 End: 32 ..GTTGACGAGGAAAAAAGAATATGTTAAAGT........................................................................................................................................................................................................................................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: + Score: 82 Start: 211 End: 240 ..................................................................................................................................................................................................................CTTCAGATATTTCTCAAGAAATCCTAAAAT........................................................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 76 HP_score: -9.6 Tail_Score: -2.80197 Start: 271 End: 294 Full_Region: TACCAAAATAAAAAA GACTGAGACA AAAA TGCCTCAGTC TAGCTTTATTATGTT ..............................................................................................................................................................................................................................................................................GACTGAGACAAAAATGCCTCAGTC.................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -11.8 Tail_Score: -5.83211 Start: 271 End: 294 Full_Region: aacataataaagcta gactgaggca tttt tgtctcagtc ttttttattttggta ..............................................................................................................................................................................................................................................................................gactgaggcatttttgtctcagtc.................................................................................................................................. Find igs database================================================ Query_seq: SSA_1594:SSA_1595|SSA_1594:SSA_1595:Metalloendopeptidase, putative:Tellurite resistance, putative:<-<-:1600617..1601040 424 ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Intra-Species Hit: Count: 1 Min: 1 Max: 424 Len: 424 Subject: NC_009009_SSA_1594_SSA_1595|Metalloendopeptidase, putative:Tellurite resistance, putative|NEGATIVE:NEGATIVE|[1600617,1601040]|424 HSP 1 e-value: 0.0 bit: 841.0 Len: 424 Query Start:1 Query End:424 Subject Strand: POSITIVE Subject Start: 1 Subject End: 424 ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt ttactttaacatattcttttttcctcgtcaacagaaaactgaaggtctggaaaaatttgccgcaagaagagagaataaggtcattcagtctgcttttaaaagtgatctgctctagactcacacttaaagaaaaactaaagtttctatcagctgtcctgttgcttccagctcttttctctcctccttgcactttctagtatagcttccaaacttcagatatttctcaagaaatcctaaaatatttctaaagttttttaccaaaataaaaaagactgagacaaaaatgcctcagtctagctttattatgttcaaaaaatattgtggctgagacaatttaaattgtctatcagaccttttaaacctgaacctcaaaataagtgggccaagctaaattttaaaaaatcaagttctgtcctactgtctt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUACUUUAACAUAUUCUUUUUUCCUCGUCAACAGAAAACUGAAGGUCUGGAAAAAUUUGCCGCAAGAAGAGAGAAUAAGGUCAUUCAGUCUGCUUUUAAAAGUGAUCUGCUCUAGACUCACACUUAAAGAAAAACUAAAGUUUCUAUCAGCUGUCCUGUUGCUUCCAGCUCUUUUCUCUCCUCCUUGCACUUUCUAGUAUAGCUUCCAAACUUCAGAUAUUUCUCAAGAAAUCCUAAAAUAUUUCUAAAGUUUUUUACCAAAAUAAAAAAGACUGAGACAAAAAUGCCUCAGUCUAGCUUUAUUAUGUUCAAAAAAUAUUGUGGCUGAGACAAUUUAAAUUGUCUAUCAGACCUUUUAAACCUGAACCUCAAAAUAAGUGGGCCAAGCUAAAUUUUAAAAAAUCAAGUUCUGUCCUACUGUCUU ...............................(((......((((((((((........(((((((((.(((((((..(((.....(((.(((.........((((((((...)))).))))......(((((........)))))..)))))).)))((((....))))....))))))).)).(((.((....(((((.(((....((((((.(((.(((((....)))))...........))).))))))................((((((((.((....)).)))))))).))).))))).)).)))......)))))))..(((((((....))))))).)))))))))).....)))............((((((.((((((..((((....))))..)))).)).))))))..... (-91.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGACAGUAGGACAGAACUUGAUUUUUUAAAAUUUAGCUUGGCCCACUUAUUUUGAGGUUCAGGUUUAAAAGGUCUGAUAGACAAUUUAAAUUGUCUCAGCCACAAUAUUUUUUGAACAUAAUAAAGCUAGACUGAGGCAUUUUUGUCUCAGUCUUUUUUAUUUUGGUAAAAAACUUUAGAAAUAUUUUAGGAUUUCUUGAGAAAUAUCUGAAGUUUGGAAGCUAUACUAGAAAGUGCAAGGAGGAGAGAAAAGAGCUGGAAGCAACAGGACAGCUGAUAGAAACUUUAGUUUUUCUUUAAGUGUGAGUCUAGAGCAGAUCACUUUUAAAAGCAGACUGAAUGACCUUAUUCUCUCUUCUUGCGGCAAAUUUUUCCAGACCUUCAGUUUUCUGUUGACGAGGAAAAAAGAAUAUGUUAAAGUAA ..(((((.(((((.((((((((..(((((((((..((.........)).))))))))).))))))))..((((((((..(((((((....)))))))..(((....(((((((((..((.(((((((..(((((((((((....))))))))))).))))))).)).....((((((((((.((.(((((((....))))))).)).))))))))))...........)))))))))..((((((((((((...(((((............))))).........((((((((.(.((((((.((((.(((.....))))))).)))))).).))))))))........))))))))))))..))).........))))))))..))))))))))............................. (-119.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 888 70 21 161 112 NC_009009:SSA_1594|5end_Metalloendopeptidase,_putative_1600526..1600756_NEGATIVE 438 250 207 181 133 NC_009009:SSA_0407|5end_ABC-type_multidrug_transport_system_(3-component_subtilin_immunity_exporter),_ATPase_component,_putative_403843..404073_NEGATIVE 410 250 201 177 131 NC_009009:SSA_0409|5end_ABC-type_multidrug_transport_system,_ATPase_component,_putative_405605..405835_NEGATIVE 364 310 263 227 178 NC_009009:SSA_1949|5end_AliA_protein,_putative_1944373..1944603_NEGATIVE 363 220 178 170 128 NC_009009:SSA_0412|5end_ABC-type_multidrug_transport_system_(3-component_subtilin_immunity_exporter),_ATPase_component,_putative_408163..408393_NEGATIVE 362 195 151 65 16 NC_009009:SSA_0950|5end_hypothetical_protein_956302..956532_POSITIVE 348 208 161 180 135 NC_009009:SSA_1010|5end_UDP-glucose_4-epimerase,_putative_1012739..1012969_POSITIVE 339 309 263 69 25 NC_009009:SSA_1575|5end_Glycosyl_transferase,_putative_1579110..1579340_NEGATIVE 339 120 79 65 17 NC_009009:SSA_0568|5end_Aspartyl-tRNA_synthetase_1,_putative_561674..561904_POSITIVE 338 189 149 42 1 NC_009009:SSA_2228|5end_Acetyltransferase_(GNAT)_family,_putative_2228768..2228998_NEGATIVE 338 186 146 103 58 NC_009009:SSA_2175|5end_hypothetical_protein_2174042..2174272_NEGATIVE 336 172 140 70 27 NC_009009:SSA_0487|5end_Delta-aminolevulinic_acid_dehydratase,_putative_487349..487579_POSITIVE 334 209 161 124 84 NC_009009:SSA_0193|5end_CAAX_amino_terminal_protease_family_protein,_putative_194318..194548_POSITIVE 330 188 148 125 89 NC_009009:SSA_0894|5end_ATP-binding_cassette_protein,_putative_885779..886009_POSITIVE 327 187 145 195 148 NC_009009:SSA_1402|5end_ABC-type_multidrug_transport_system,_ATPase_and_permease_components,_putative_1416220..1416450_NEGATIVE 322 187 148 165 123 NC_009009:SSA_1560|5end_Structural_maintenance_of_chromosome_protein_(chromosome_segregation_ATPase),_putative_1565194..1565424_NEGATIVE 319 181 149 64 28 NC_009009:SSA_2106|5end_Flavin_monoxygenase,_putative_2109064..2109294_NEGATIVE 319 199 160 109 63 NC_009009:SSA_1208|5end_23S_rRNA_pseudouridine_synthase,_putative_1233185..1233415_NEGATIVE 317 208 162 123 85 NC_009009:SSA_0888|5end_Magnesium/cobalt_transporter,_putative_881329..881559_POSITIVE 315 176 131 89 38 NC_009009:SSA_0510|5end_L-threonine-O-3-phosphate_decarboxylase,_putative_508523..508753_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 421 310 264 58 12 NC_009009:SSA_0956|3end_surface_protein_D_965418..965568_POSITIVE 362 195 151 78 29 NC_009009:SSA_0949|3end_hypothetical_protein_956395..956545_POSITIVE 336 172 140 83 40 NC_009009:SSA_0486|3end_Uroporphyrinogen-III_synthase,_putative_487442..487592_POSITIVE 330 188 148 138 102 NC_009009:SSA_0893|3end_ATP-binding_cassette_transporter-like_protein,_putative_885872..886022_POSITIVE 329 195 151 151 100 NC_009009:SSA_1985|3end_hypothetical_protein_1979623..1979773_NEGATIVE 328 169 139 120 88 NC_009009:SSA_1005|3end_Sugar_ABC_transporter,_permease_protein,_putative_1006941..1007091_POSITIVE 319 199 160 112 66 NC_009009:SSA_1209|3end_Inorganic_polyphosphate/ATP-NAD_kinase,_putative_1233182..1233332_NEGATIVE 311 250 219 32 1 NC_009009:SSA_0410|3end_hypothetical_protein_405750..405900_NEGATIVE 310 176 136 63 16 NC_009009:SSA_0834|3end_Accessory_secretory_protein_Asp2,_putative_821119..821269_POSITIVE 307 189 141 142 96 NC_009009:SSA_2141|3end_Argininosuccinate_lyase,_putative_2140819..2140969_NEGATIVE 307 189 151 128 91 NC_009009:SSA_0894|3end_ATP-binding_cassette_protein,_putative_886533..886683_POSITIVE 307 195 152 78 29 NC_009009:SSA_0142|3end_hypothetical_protein_137489..137639_POSITIVE 306 250 215 35 1 NC_009009:SSA_0408|3end_hypothetical_protein_403989..404139_NEGATIVE 305 310 265 101 58 NC_009009:SSA_1491|3end_Methionine_aminopeptidase_A,_putative_1496780..1496930_NEGATIVE 302 184 141 150 106 NC_009009:SSA_1449|3end_Histidinol-phosphate/aromatic_aminotransferase_and_cobyric_acid_decarboxylase,_putative_1450873..1451023_NEGATIVE 301 190 141 150 89 NC_009009:SSA_0715|3end_DNA_uptake_protein,_putative_701486..701636_POSITIVE 298 210 161 139 96 NC_009009:SSA_1757|3end_hypothetical_protein_1745282..1745432_NEGATIVE 296 189 142 151 100 NC_009009:SSA_1900|3end_N_utilization_substance_protein_A,_putative_1894750..1894900_NEGATIVE 295 165 124 132 95 NC_009009:SSA_1372|3end_hypothetical_protein_1388946..1389096_NEGATIVE 295 188 148 108 56 NC_009009:SSA_1153|3end_HemK_protein,_putative_1182748..1182898_POSITIVE