Origin IGS: atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttatcctcctttatctgacctctatcttaacactttttcatgcaaaagatagaaagcaagctcatattattccagtctctgcactaatgcttttttttcaatccaaaaggtggtataatgagaattgaatttttagaagtttaatggtgaaat Mask Tandem Repeat Region ================================================ atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Find is-nt database================================================ Query_seq: SSA_1522:SSA_1523|SSA_1522:SSA_1523:Cell division protein FtsW, putative:Glutathione peroxidase, putative:<-->:1532053..1532206 154 atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1522:SSA_1523|SSA_1522:SSA_1523:Cell division protein FtsW, putative:Glutathione peroxidase, putative:<-->:1532053..1532206 154 atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1522:SSA_1523|SSA_1522:SSA_1523:Cell division protein FtsW, putative:Glutathione peroxidase, putative:<-->:1532053..1532206 154 atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Predict ORF larger than 30AA ================================================ Protein_Len: 41 Strand: - Start: 29 End: 151 ............................ N E N Y W R K S Q F F L M L A S V P I I H A Q K R D K A H F L T L I S T L Y L L M ... atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_1522:SSA_1523|SSA_1522:SSA_1523:Cell division protein FtsW, putative:Glutathione peroxidase, putative:<-->:1532053..1532206 154 atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Intra-Species Hit: Count: 1 Min: 1 Max: 154 Len: 154 Subject: NC_009009_SSA_1522_SSA_1523|Cell division protein FtsW, putative:Glutathione peroxidase, putative|NEGATIVE:POSITIVE|[1532053,1532206]|154 HSP 1 e-value: 5.0E-68 bit: 258.0 Len: 154 Query Start:1 Query End:154 Subject Strand: POSITIVE Subject Start: 1 Subject End: 154 atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgnnnnnnnngcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac atttcaccattaaacttctaaaaattcaattctcattataccaccttttggattgaaaaaaaagcattagtgcagagactggaataatatgagcttgctttctatcttttgcatgaaaaagtgttaagatagaggtcagataaaggaggataac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUCACCAUUAAACUUCUAAAAAUUCAAUUCUCAUUAUACCACCUUUUGGAUUGAAAAAAAAGCAUUAGUGCAGAGACUGGAAUAAUAUGAGCUUGCUUUCUAUCUUUUGCAUGAAAAAGUGUUAAGAUAGAGGUCAGAUAAAGGAGGAUAAC .........(((..(((((............((((((((.(((..(((((.(((((..........))))).)))))..))).)))..))))).(((.((((((((((..((((......)))).)))))))))).)))....)))))..))). (-31.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUAUCCUCCUUUAUCUGACCUCUAUCUUAACACUUUUUCAUGCAAAAGAUAGAAAGCAAGCUCAUAUUAUUCCAGUCUCUGCACUAAUGCUUUUUUUUCAAUCCAAAAGGUGGUAUAAUGAGAAUUGAAUUUUUAGAAGUUUAAUGGUGAAAU ..........((((((.....((((((((..((........))...))))))))....(((((..((..((((...((((.......(((((.((((((......)))))).)))))...))))...))))...))..)))))...)))))).. (-22.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 831 140 91 231 182 NC_009009:SSA_1522|5end_Cell_division_protein_FtsW,_putative_1531962..1532192_NEGATIVE 295 103 63 143 103 NC_009009:SSA_0490|5end_Cobalamin_5'-phosphate_synthase,_putative_490236..490466_POSITIVE 277 149 106 206 149 NC_009009:SSA_1914|5end_hypothetical_protein_1905195..1905425_NEGATIVE 267 112 73 88 39 NC_009009:SSA_0539|5end_PduO_protein,_putative_534233..534463_POSITIVE 267 140 91 225 165 NC_009009:SSA_0339|5end_hypothetical_protein_333950..334180_NEGATIVE 261 116 72 184 145 NC_009009:SSA_0496|5end_Succinylglutamate_desuccinylase_/_Aspartoacylase_family,_putative_495946..496176_POSITIVE 259 134 93 223 186 NC_009009:SSA_0746|5end_Glucosamine-6-phosphate_deaminase,_putative_727078..727308_POSITIVE 258 114 71 228 184 NC_009009:SSA_0589|5end_Acetylornithine_deacetylase/succinyl-diaminopimelate_desuccinylase_(M20/M25/M40_family_peptidase),_putative_580745..580975_POSITIVE 254 107 63 54 12 NC_009009:SSA_2069|5end_Prolyl-tRNA_synthetase,_putative_2073252..2073482_NEGATIVE 249 139 91 208 158 NC_009009:SSA_2099|5end_ABC-type_arginine/histidine_transporter,_permease_protein,_putative_2104313..2104543_NEGATIVE 248 110 74 154 112 NC_009009:SSA_1173|5end_Lipoate_protein_ligase_A,_putative_1201081..1201311_NEGATIVE 245 150 103 176 123 NC_009009:SSA_1811|5end_6-phosphogluconate_dehydrogenase,_putative_1796830..1797060_NEGATIVE 243 117 73 54 5 NC_009009:SSA_0576|5end_Conserved_GTPase,_putative_570039..570269_POSITIVE 242 53 15 165 123 NC_009009:SSA_2379|5end_Signal_transduction_protein_with_a_C-terminal_ATPase/histidine_kinase_domain,_putative_2385233..2385463_NEGATIVE 242 107 62 138 92 NC_009009:SSA_1094|5end_GTP-binding_protein,_putative_1111576..1111806_POSITIVE 241 113 79 182 137 NC_009009:SSA_1868|5end_ABC-type_arginine/histidine_transport_system,_permease_component,_putative_1863902..1864132_NEGATIVE 240 75 31 133 84 NC_009009:SSA_0680|5end_Serine/threonine_protein_phosphatase,_putative_662425..662655_POSITIVE 240 101 62 205 163 NC_009009:SSA_0221|5end_PTS_system,_mannose-specific_IIC_component,_putative_216370..216600_POSITIVE 239 135 93 141 81 NC_009009:SSA_1821|5end_Acetyltransferase_(N-acetylase_of_ribosomal_proteins),_putative_1810610..1810840_NEGATIVE 239 116 73 93 47 NC_009009:SSA_1743|5end_ABC-type_Fe3+-siderophore_transport_system,_permease_component,_putative_1730170..1730400_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 295 103 63 146 106 NC_009009:SSA_0489|3end_Adenosylcobinamide_kinase_/_adenosylcobinamide_phosphate_guanyltransferase,_putative_490319..490469_POSITIVE 277 149 106 125 68 NC_009009:SSA_1915|3end_hypothetical_protein_1905276..1905426_NEGATIVE 258 114 71 110 66 NC_009009:SSA_0588|3end_L-cystine_ABC_transporter,_substrate-binding_component,_putative_580707..580857_POSITIVE 250 150 101 84 27 NC_009009:SSA_0070|3end_Acetylxylan_esterase,_putative_74914..75064_POSITIVE 248 110 74 87 45 NC_009009:SSA_1174|3end_Dihydrolipoamide_dehydrogenase,_putative_1201148..1201298_NEGATIVE 243 117 73 51 2 NC_009009:SSA_0575|3end_Hydrolase,_HAD_superfamily,_putative_570116..570266_POSITIVE 242 53 15 137 95 NC_009009:SSA_2394|3end_Competence_stimulating_peptide_2385261..2385411_NEGATIVE 242 107 62 127 81 NC_009009:SSA_1093|3end_ATP-dependent_Clp_protease_ATP-binding_subunit_clpX,_putative_1111645..1111795_POSITIVE 240 139 91 57 6 NC_009009:SSA_1149|3end_Glycosyl_hydrolase,_family_1,_putative_1179876..1180026_POSITIVE 240 75 31 80 31 NC_009009:SSA_0679|3end_DNA_repair_and_genetic_recombination,_putative_662452..662602_POSITIVE 237 112 71 64 25 NC_009009:SSA_1515|3end_hypothetical_protein_1521770..1521920_NEGATIVE 234 116 72 83 30 NC_009009:SSA_1797|3end_Aminodeoxychorismate_lyase,_putative_1778448..1778598_NEGATIVE 233 82 49 69 34 NC_009009:SSA_1552|3end_hypothetical_protein_1555130..1555280_POSITIVE 229 137 92 132 93 NC_009009:SSA_0462|3end_ABC-type_multidrug_transport_system_(phospholipid,_LPS,_lipid_A_and_drug_exporter),_ATPase_and_permease_components,_putative_466652..466802_POSITIVE 228 99 63 144 103 NC_009009:SSA_0756|3end_Alanyl-tRNA_synthetase,_putative_739434..739584_POSITIVE 225 112 90 29 2 NC_009009:SSA_0538|3end_PduH_protein,_putative_534254..534404_POSITIVE 220 104 61 83 33 NC_009009:SSA_1974|3end_ABC-type_multidrug_transport_system,_permease_component,_putative_1969512..1969662_NEGATIVE 220 148 101 82 21 NC_009009:SSA_0714|3end_hypothetical_protein_700649..700799_POSITIVE 218 107 61 81 37 NC_009009:SSA_2108|3end_Glyceraldehyde_3-phosphate_dehydrogenase,_putative_2111457..2111607_NEGATIVE 218 108 63 92 33 NC_009009:SSA_0101|3end_Conserved_uncharacterized_protein_104113..104263_POSITIVE