Origin IGS: tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaattcaaaatactagaagctggaataaatgcttctagtattttttactactttttaacgcttattcatttctgtaaataaaaacccataagcaagaaatgcataaacagattctttgattcttaaaacaaacttaagataagcttaaaaaaggcttaagataagcttaaaataaacttaaacaaaaaatcgaaatgagagggtgagaagtttttataaataaacaacaaaaataatttacatagtgtaaaccaaattataaacttttttctattattttggttcgttaaaaagaatattttatagatttttaaagctaaaaaacagaaaaataaacaaaaagacgaactataaaaaattacccataaaaataatttacaccttgttaactaaaatgcttgcattcaaaaatttcttgtgctataattcgtcttgagggtgagatatgcataaaaaagtattttcaagcatattgatttctttatttctaaatcaaatgttataaaaacatttatgtgcaacctttaaataggtggattcactaacta Mask Tandem Repeat Region ================================================ tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Find is-nt database================================================ Query_seq: SSA_1275:SSA_1276|SSA_1275:SSA_1276:hypothetical protein:Conserved uncharacterized protein:<-<-:1297697..1298245 549 tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1275:SSA_1276|SSA_1275:SSA_1276:hypothetical protein:Conserved uncharacterized protein:<-<-:1297697..1298245 549 tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1275:SSA_1276|SSA_1275:SSA_1276:hypothetical protein:Conserved uncharacterized protein:<-<-:1297697..1298245 549 tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 28 End: 141 ........................... M H I N V F I T F D L E I K K S I C L K I L F Y A Y L T L K T N Y S T R N F ........................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 190 End: 390 ............................................................................................................................................................................................. M F Y S S S F C L F F C F L A L K I Y K I F F L T N Q N N R K K F I I W F T L C K L F L L F I Y K N F S P S H F D F L F ............................................................................................................................................................... Protein_Len: 46 Strand: - Start: 116 End: 253 ................................................................................................................... S S N Y C L F N K F A L M K T L L T Y I I K I P L K K Y N T K K N I K R N K L K L F R Y F M ........................................................................................................................................................................................................................................................................................................ tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 122 End: 151 .........................................................................................................................CTTGCATTCAAAAATTTCTTGTGCTATAAT.............................................................................................................................................................................................................................................................................................................................................................................................................. PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 376 End: 405 .......................................................................................................................................................................................................................................................................................................................................................................................CTTAAAAAAGGCTTAAGATAAGCTTAAAAT................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 72 HP_score: -4.1 Tail_Score: -5.44198 Start: 295 End: 315 Full_Region: AAAAAGTTTATAATT TGGTTTACA CTA TGTAAATTA TTTTTGTTGTTTATT ......................................................................................................................................................................................................................................................................................................TGGTTTACACTATGTAAATTA.......................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 73 HP_score: -5.8 Tail_Score: -4.74161 Start: 298 End: 312 Full_Region: AAGTTTATAATTTGG TTTACA CTA TGTAAA TTATTTTTGTTGTTT .........................................................................................................................................................................................................................................................................................................TTTACACTATGTAAA............................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 44 HP_score: -2.9 Tail_Score: -3.38836 Start: 401 End: 415 Full_Region: TATCTTAAGCCTTTT TTAAG CTTAT CTTAA GTTTGTTTTAAGAAT ................................................................................................................................................................................................................................................................................................................................................................................................................TTAAGCTTATCTTAA...................................................................................................................................... Find igs database================================================ Query_seq: SSA_1275:SSA_1276|SSA_1275:SSA_1276:hypothetical protein:Conserved uncharacterized protein:<-<-:1297697..1298245 549 tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaaattatttttatgggtaattttttatagttcgtctttttgtttatttttctgttttttagctttaaaaatctataaaatattctttttaacgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcgattttttgtttaagtttattttaagcttatcttaagccttttttaagcttatcttaagtttgttttaagaatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta Intra-Species Hit: Count: 1 Min: 1 Max: 549 Len: 549 Subject: NC_009009_SSA_1275_SSA_1276|hypothetical protein:Conserved uncharacterized protein|NEGATIVE:NEGATIVE|[1297697,1298245]|549 HSP 1 e-value: 4.0E-93 bit: 343.0 Len: 173 Query Start:1 Query End:173 Subject Strand: POSITIVE Subject Start: 1 Subject End: 173 tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaa........................................................................................................................................................................................................................................................................................................................................................................................ tagttagtgaatccacctatttaaaggttgcacataaatgtttttataacatttgatttagaaataaagaaatcaatatgcttgaaaatacttttttatgcatatctcaccctcaagacgaattatagcacaagaaatttttgaatgcaagcattttagttaacaaggtgtaa........................................................................................................................................................................................................................................................................................................................................................................................ HSP 2 e-value: 1.0E-62 bit: 242.0 Len: 122 Query Start:428 Query End:549 Subject Strand: POSITIVE Subject Start: 428 Subject End: 549 ...........................................................................................................................................................................................................................................................................................................................................................................................................................................aatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta ...........................................................................................................................................................................................................................................................................................................................................................................................................................................aatcaaagaatctgtttatgcatttcttgcttatgggtttttatttacagaaatgaataagcgttaaaaagtagtaaaaaatactagaagcatttattccagcttctagtattttgaattta HSP 3 e-value: 2.0E-48 bit: 194.0 Len: 98 Query Start:262 Query End:359 Subject Strand: POSITIVE Subject Start: 262 Subject End: 359 .....................................................................................................................................................................................................................................................................acgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcga.............................................................................................................................................................................................. .....................................................................................................................................................................................................................................................................acgaaccaaaataatagaaaaaagtttataatttggtttacactatgtaaattatttttgttgtttatttataaaaacttctcaccctctcatttcga.............................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUAGUGAAUCCACCUAUUUAAAGGUUGCACAUAAAUGUUUUUAUAACAUUUGAUUUAGAAAUAAAGAAAUCAAUAUGCUUGAAAAUACUUUUUUAUGCAUAUCUCACCCUCAAGACGAAUUAUAGCACAAGAAAUUUUUGAAUGCAAGCAUUUUAGUUAACAAGGUGUAAAUUAUUUUUAUGGGUAAUUUUUUAUAGUUCGUCUUUUUGUUUAUUUUUCUGUUUUUUAGCUUUAAAAAUCUAUAAAAUAUUCUUUUUAACGAACCAAAAUAAUAGAAAAAAGUUUAUAAUUUGGUUUACACUAUGUAAAUUAUUUUUGUUGUUUAUUUAUAAAAACUUCUCACCCUCUCAUUUCGAUUUUUUGUUUAAGUUUAUUUUAAGCUUAUCUUAAGCCUUUUUUAAGCUUAUCUUAAGUUUGUUUUAAGAAUCAAAGAAUCUGUUUAUGCAUUUCUUGCUUAUGGGUUUUUAUUUACAGAAAUGAAUAAGCGUUAAAAAGUAGUAAAAAAUACUAGAAGCAUUUAUUCCAGCUUCUAGUAUUUUGAAUUUA .((((((.((((..((((......))))......((((((((.....))))))))....(((((((((.(((...((((((.((((((....)))))).)))))).........(((((((((((((((((...........(((((....)))))...........))))(((((((((....)))))))))..)))))))))))))))).)))))))))..)))).))))))................((((((((((((((.....((((((((((((..((((((((...((((....)))))))))))).)))))))))))).......................((((((((((((((.(..(((..(((...((((((((..............))))))))...)))..)))..).))))))).((((((((((....(((.....)))..)))))))))).......))))))).....)))))))))).)))).((((((((((((((..........))))))))))))))....... (-116.19) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUUCAAAAUACUAGAAGCUGGAAUAAAUGCUUCUAGUAUUUUUUACUACUUUUUAACGCUUAUUCAUUUCUGUAAAUAAAAACCCAUAAGCAAGAAAUGCAUAAACAGAUUCUUUGAUUCUUAAAACAAACUUAAGAUAAGCUUAAAAAAGGCUUAAGAUAAGCUUAAAAUAAACUUAAACAAAAAAUCGAAAUGAGAGGGUGAGAAGUUUUUAUAAAUAAACAACAAAAAUAAUUUACAUAGUGUAAACCAAAUUAUAAACUUUUUUCUAUUAUUUUGGUUCGUUAAAAAGAAUAUUUUAUAGAUUUUUAAAGCUAAAAAACAGAAAAAUAAACAAAAAGACGAACUAUAAAAAAUUACCCAUAAAAAUAAUUUACACCUUGUUAACUAAAAUGCUUGCAUUCAAAAAUUUCUUGUGCUAUAAUUCGUCUUGAGGGUGAGAUAUGCAUAAAAAAGUAUUUUCAAGCAUAUUGAUUUCUUUAUUUCUAAAUCAAAUGUUAUAAAAACAUUUAUGUGCAACCUUUAAAUAGGUGGAUUCACUAACUA .......((((((((((((((..........))))))))))))))......((((((((((......((((..(((((((....((((....(((.....))).......((((.((((..((((((.......))))))((((.(((....(((((((...)))))))....))).))))..)))).)))).........)))).....((((........))))..........))))))).))))..(((((((.((...............)).)))))))))))))))))..((((((...(.((((((....)))))).)...))))))......((((((((.((((..((((((..........)))))).(((............((((....))))............))).)))).))))))))...((((((...((((((.(((.((.(((...(((((.(((((((..........))))))))))))..))).)).))).))))))((((......))))...))))))..... (-97.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 787 120 71 211 162 NC_009009:SSA_1275|5end_hypothetical_protein_1297606..1297836_NEGATIVE 270 468 424 230 194 NC_009009:SSA_1880|5end_Glycyl-tRNA_synthetase_alpha_chain,_putative_1875182..1875412_NEGATIVE 268 130 81 95 32 NC_009009:SSA_1995|5end_hypothetical_protein_1993358..1993588_NEGATIVE 268 450 404 115 57 NC_009009:SSA_1861|5end_Aminopeptidase_C,_putative_1857902..1858132_NEGATIVE 243 360 316 116 73 NC_009009:SSA_2013|5end_hypothetical_protein_2013793..2014023_NEGATIVE 243 361 337 183 156 NC_009009:SSA_1288|5end_hypothetical_protein_1307578..1307808_NEGATIVE 242 138 94 139 86 NC_009009:SSA_0356|5end_Dipeptidase,_putative_349259..349489_POSITIVE 239 424 387 166 131 NC_009009:SSA_2193|5end_ADP-ribose_pyrophosphatase,_putative_2194793..2195023_NEGATIVE 239 119 74 112 56 NC_009009:SSA_1078|5end_hypothetical_protein_1095742..1095972_POSITIVE 238 130 81 120 71 NC_009009:SSA_1504|5end_hypothetical_protein_1506947..1507177_NEGATIVE 235 385 341 208 160 NC_009009:SSA_1019|5end_Collagen-binding_surface_protein,_putative_1032877..1033107_POSITIVE 234 126 81 120 77 NC_009009:SSA_0023|5end_Aspartate_aminotransferase,_putative_28529..28759_POSITIVE 233 390 341 150 97 NC_009009:SSA_1237|5end_Uracil-DNA_glycosylase,_putative_1262366..1262596_NEGATIVE 232 136 92 229 188 NC_009009:SSA_0714|5end_hypothetical_protein_700042..700272_POSITIVE 227 189 146 123 74 NC_009009:SSA_1319|5end_hypothetical_protein_1335885..1336115_POSITIVE 227 489 445 123 70 NC_009009:SSA_0387|5end_Transcriptional_regulator,_GntR_family,_putative_382343..382573_NEGATIVE 226 413 384 229 201 NC_009009:SSA_0469|5end_Precorrin-6B_methylase_2,putative_472310..472540_POSITIVE 225 390 341 193 147 NC_009009:SSA_1415|5end_Oxidoreductase,_putative_1426508..1426738_NEGATIVE 225 139 97 124 85 NC_009009:SSA_0246|5end_Conserved_uncharacterized_protein_239703..239933_POSITIVE 223 129 89 144 97 NC_009009:SSA_0596|5end_hypothetical_protein_586913..587143_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 250 445 405 66 24 NC_009009:SSA_0511|3end_Propanediol_utilization_kinase,_putative_510556..510706_POSITIVE 242 138 94 60 7 NC_009009:SSA_0355|3end_DNA_mismatch_repair_protein,_putative_349260..349410_POSITIVE 239 424 387 111 76 NC_009009:SSA_2194|3end_Nicotinamide_mononucleotide_transporter,_putative_2194848..2194998_NEGATIVE 239 119 74 119 63 NC_009009:SSA_1077|3end_N-acetyl-glucosamine_matabolism,_putative_1095829..1095979_POSITIVE 233 390 341 114 61 NC_009009:SSA_1238|3end_hypothetical_protein_1262402..1262552_NEGATIVE 232 136 92 135 94 NC_009009:SSA_0713|3end_1-acyl-sn-glycerol-3-phosphate_acyltransferase,_putative_700028..700178_POSITIVE 227 235 191 92 41 NC_009009:SSA_2008|3end_Molecular_chaperone_GrpE_(HSP-70_cofactor),_putative_2009526..2009676_NEGATIVE 223 420 371 143 83 NC_009009:SSA_0906|3end_CshA-like_fibrillar_surface_protein_C_917411..917561_POSITIVE 223 129 89 134 87 NC_009009:SSA_0595|3end_hypothetical_protein_586983..587133_POSITIVE 221 358 316 120 81 NC_009009:SSA_1493|3end_hypothetical_protein_1498929..1499079_NEGATIVE 217 235 191 110 70 NC_009009:SSA_1107|3end_Lipid/multidrug/protein-type_ABC_exporter,_ATP_binding/membrane-spanning_protein,_putative_1133784..1133934_POSITIVE 217 210 167 115 80 NC_009009:SSA_1064|3end_hypothetical_protein_1080325..1080475_NEGATIVE 213 38 1 48 14 NC_009009:SSA_1009|3end_Galactose-1-phosphate-uridylyltransferase,_putative_1012815..1012965_POSITIVE 211 387 342 84 33 NC_009009:SSA_1106|3end_IgA-specific_metalloendopeptidase_1131678..1131828_POSITIVE 208 377 339 120 75 NC_009009:SSA_1331|3end_Conserved_uncharacterized_protein_1346473..1346623_NEGATIVE 207 230 182 92 44 NC_009009:SSA_1879|3end_Glycyl-tRNA_synthetase_beta_chain,_putative_1872223..1872373_NEGATIVE 207 410 364 94 48 NC_009009:SSA_1188|3end_Ribonuclease_HII,_putative_1216055..1216205_NEGATIVE 207 440 393 52 5 NC_009009:SSA_0631|3end_Tryptophan_synthase_beta_chain,_putative_621133..621283_POSITIVE 204 385 346 100 52 NC_009009:SSA_1298|3end_Maltose/maltodextrin_ABC_transporter,_sugar-binding_protein_MalX,_putative_1316627..1316777_POSITIVE 204 457 414 141 82 NC_009009:SSA_1185|3end_DNA_processing_Smf_protein,_putative_1213690..1213840_NEGATIVE