Origin IGS: gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtataatgaagaaaaggctttcttatgaattatattacataaattattttatgtaatataatcttgtgtagagggtcaaagtagctaaaattttactaattacagacaatcgttattcggatcgattttatccgatgagcgaattagtaagcctacaaggttgattgccgatagcggtaaccgtagcaacttgagataggtttgtatccaagttactgtagagttgaacatgggctaaagtctgtgtgaaaaagataaacttttctagaaacgaaaatttcttcgttaagttttctattttcactttgatttttaacgccttagtatcatgtaattgaattcggacggaggattgcgaaaaaaagatagatttccttgtgttcattgaacacaaggaaatctcctattttttcattgtcctctgagtccttaatatcttataatagaattggagaggtc Mask Tandem Repeat Region ================================================ gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Find is-nt database================================================ Query_seq: SSA_1175:SSA_1176|SSA_1175:SSA_1176:Acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase, putative:Acetoin dehydrogenase, E1 component, beta subunit, putative:<-<-:1204067..1204526 460 gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_1175:SSA_1176|SSA_1175:SSA_1176:Acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase, putative:Acetoin dehydrogenase, E1 component, beta subunit, putative:<-<-:1204067..1204526 460 gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_1175:SSA_1176|SSA_1175:SSA_1176:Acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase, putative:Acetoin dehydrogenase, E1 component, beta subunit, putative:<-<-:1204067..1204526 460 gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 26 End: 130 ......................... M K D S E D N E K I G D F L V F N E H K E I Y L F F A I L R P N S I T .......................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 60 End: 329 ........................................................... M S L C S M N T R K S I F F S Q S S V R I Q L H D T K A L K I K V K I E N L T K K F S F L E K F I F F T Q T L A H V Q L ................................................................................................................................... Protein_Len: 43 Strand: + Start: 229 End: 357 .................................................................................................................................................................................................................................... M F N S T V T W I Q T Y L K L L R L P L S A I N L V G L L I R S S D K I D P N N D C L ....................................................................................................... Protein_Len: 30 Strand: - Start: 125 End: 214 ............................................................................................................................ N C S V L A N F I L T F I S F K V F F N E N R S F N I K K M ...................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: - Start: 1 End: 90 V E G I R N Y S I L S E S S L S F I P S K R T N L S C L S M .................................................................................................................................................................................................................................................................................................................................................................................. gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 398 End: 427 .............................................................................................................................................................................................................................................................................................................................................................................................................TATTACATAAATTATTTTATGTAATATAAT................................. PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 402 End: 431 .................................................................................................................................................................................................................................................................................................................................................................................................................TATTACATAAAATAATTTATGTAATATAAT............................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -12.0 Tail_Score: -5.33848 Start: 54 End: 97 Full_Region: gaggattgcgaaaaa aagatagatttccttgtgttc att gaacacaaggaaatctccta ttttttcattgtcct .....................................................aagatagatttccttgtgttcattgaacacaaggaaatctccta........................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -16.8 Tail_Score: -5.42259 Start: 56 End: 95 Full_Region: GACAATGAAAAAATA GGAGATTTCCTTGTGTTC AAT GAACACAAGGAAATCTATC TTTTTTTCGCAATCC .......................................................GGAGATTTCCTTGTGTTCAATGAACACAAGGAAATCTATC............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -14.8 Tail_Score: -4.29187 Start: 56 End: 94 Full_Region: gattgcgaaaaaaag atagatttccttgtgttc att gaacacaaggaaatctcc tattttttcattgtc .......................................................atagatttccttgtgttcattgaacacaaggaaatctcc.............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -18.3 Tail_Score: -4.35085 Start: 57 End: 93 Full_Region: ACAATGAAAAAATAG GAGATTTCCTTGTGTTC AAT GAACACAAGGAAATCTA TCTTTTTTTCGCAAT ........................................................GAGATTTCCTTGTGTTCAATGAACACAAGGAAATCTA............................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -18.3 Tail_Score: -3.15885 Start: 57 End: 93 Full_Region: attgcgaaaaaaaga tagatttccttgtgttc att gaacacaaggaaatctc ctattttttcattgt ........................................................tagatttccttgtgttcattgaacacaaggaaatctc............................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -20.9 Tail_Score: -3.76885 Start: 59 End: 91 Full_Region: AATGAAAAAATAGGA GATTTCCTTGTGTTC AAT GAACACAAGGAAATC TATCTTTTTTTCGCA ..........................................................GATTTCCTTGTGTTCAATGAACACAAGGAAATC................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 100 HP_score: -20.9 Tail_Score: -3.11487 Start: 59 End: 91 Full_Region: tgcgaaaaaaagata gatttccttgtgttc att gaacacaaggaaatc tcctattttttcatt ..........................................................gatttccttgtgttcattgaacacaaggaaatc................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 46 HP_score: -2.4 Tail_Score: -3.46285 Start: 175 End: 188 Full_Region: aaacttttctagaaa cgaa aatttc ttcg ttaagttttctattt ..............................................................................................................................................................................cgaaaatttcttcg................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 80 HP_score: -9.4 Tail_Score: -3.48924 Start: 397 End: 432 Full_Region: aaaggctttcttatg aattatattacataaa ttat tttatgtaatataatc ttgtgtagagggtca ............................................................................................................................................................................................................................................................................................................................................................................................................aattatattacataaattattttatgtaatataatc............................ TransTerm Strand: - Conf: 89 HP_score: -12.0 Tail_Score: -3.31905 Start: 399 End: 430 Full_Region: aggctttcttatgaa ttatattacataaa ttat tttatgtaatataa tcttgtgtagagggt ..............................................................................................................................................................................................................................................................................................................................................................................................................ttatattacataaattattttatgtaatataa.............................. Find igs database================================================ Query_seq: SSA_1175:SSA_1176|SSA_1175:SSA_1176:Acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase, putative:Acetoin dehydrogenase, E1 component, beta subunit, putative:<-<-:1204067..1204526 460 gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc Intra-Species Hit: Count: 1 Min: 1 Max: 460 Len: 460 Subject: NC_009009_SSA_1175_SSA_1176|Acetoin dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase, putative:Acetoin dehydrogenase, E1 component, beta subunit, putative|NEGATIVE:NEGATIVE|[1204067,1204526]|460 HSP 1 e-value: 0.0 bit: 870.0 Len: 460 Query Start:1 Query End:460 Subject Strand: POSITIVE Subject Start: 1 Subject End: 460 gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatcnnnnnnncgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc gacctctccaattctattataagatattaaggactcagaggacaatgaaaaaataggagatttccttgtgttcaatgaacacaaggaaatctatctttttttcgcaatcctccgtccgaattcaattacatgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaactctacagtaacttggatacaaacctatctcaagttgctacggttaccgctatcggcaatcaaccttgtaggcttactaattcgctcatcggataaaatcgatccgaataacgattgtctgtaattagtaaaattttagctactttgaccctctacacaagattatattacataaaataatttatgtaatataattcataagaaagccttttcttcattatacc HSP 2 e-value: 2.0E-8 bit: 61.9 Len: 35 Query Start:58 Query End:92 Subject Strand: NEGATIVE Subject Start: 58 Subject End: 92 .........................................................agatttccttgtgttcaatgaacacaaggaaatct................................................................................................................................................................................................................................................................................................................................................................................ .........................................................agatttccttgtgttcattgaacacaaggaaatct................................................................................................................................................................................................................................................................................................................................................................................ Inter-species Hit: Count: 1 Min: 129 Max: 236 Len: 108 Subject: NC_004350_SMU.2011_SMU.2012|50S ribosomal protein L6:30S ribosomal protein S8|NEGATIVE:NEGATIVE|[1887284,1887695]|412 HSP 1 e-value: 2.0E-11 bit: 71.9 Len: 108 Query Start:129 Query End:236 Subject Strand: POSITIVE Subject Start: 61 Subject End: 168 ................................................................................................................................catgatactaaggcgttaaaaatcaaagtgaaaatagaaaacttaacgaagaaattttcgtttctagaaaagtttatctttttcacacagactttagcccatgttcaa................................................................................................................................................................................................................................ ................................................................................................................................catgatacaaaggagtcaaaagccaaagtgaaaatagataacttgacgaagaaacacaggtttctaggaaaatttatctttttcacacaggttttaacctatgttcaa................................................................................................................................................................................................................................ Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUGAUACUAAGGCGUUAAAAAUCAAAGUGAAAAUAGAAAACUUAACGAAGAAAUUUUCGUUUCUAGAAAAGUUUAUCUUUUUCACACAGACUUUAGCCCAUGUUCAA ..(((.((...((.((((((..((...(((((((.((((((((((((((((....))))))).......)))))).))))))))))...)).))))))))..))))). (-22.01) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGAACAUGGGCUAAAGUCUGUGUGAAAAAGAUAAACUUUUCUAGAAACGAAAAUUUCUUCGUUAAGUUUUCUAUUUUCACUUUGAUUUUUAACGCCUUAGUAUCAUG .(((((..((((.((((((...((((((((((.((((((.......((((((......))))))))))))))).)))))))...))))))....))))..)).))).. (-26.41) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 358 48 4 157 113 NC_009009:SSA_1313|5end_hypothetical_protein_1330548..1330778_NEGATIVE 245 97 66 188 158 NC_009009:SSA_1226|5end_ParC,_putative_1251233..1251463_NEGATIVE 219 97 56 73 37 NC_009009:SSA_0781|5end_Mannose-6-phosphate_isomerase,_putative_765373..765603_POSITIVE 215 99 70 169 137 NC_009009:SSA_1863|5end_NH(3)-dependent_NAD(+)_synthetase,_putative_1859413..1859643_NEGATIVE 204 37 4 56 23 NC_009009:SSA_1258|5end_Purine_nucleoside_phosphorylase,_family_1,_putative_1284652..1284882_NEGATIVE 202 98 60 89 54 NC_009009:SSA_1080|5end_Transcriptional_repressor_of_the_fructose_operon,_DeoR_family,_putative_1097538..1097768_POSITIVE 201 100 53 61 7 NC_009009:SSA_2165|5end_ABC-type_oligopeptide_transporter,_periplasmic_component,_putative_2161734..2161964_POSITIVE 201 98 58 129 94 NC_009009:SSA_1709|5end_hypothetical_protein_1698414..1698644_NEGATIVE 201 98 58 224 189 NC_009009:SSA_1708|5end_hypothetical_protein_1698319..1698549_NEGATIVE 197 37 4 131 98 NC_009009:SSA_0914|5end_Phenylalanyl-tRNA_synthetase_beta_chain,_putative_924952..925182_POSITIVE 192 100 52 99 36 NC_009009:SSA_0529|5end_Ethanolamine_utilization_protein,_putative_525945..526175_POSITIVE 190 91 55 77 34 NC_009009:SSA_0380|5end_Enzyme_of_poly-gamma-glutamate_biosynthesis_(capsule_formation),_putative_370916..371146_POSITIVE 187 100 52 103 48 NC_009009:SSA_1103|5end_hypothetical_protein_1123833..1124063_POSITIVE 186 99 56 140 95 NC_009009:SSA_2193|5end_ADP-ribose_pyrophosphatase,_putative_2194793..2195023_NEGATIVE 185 60 13 230 180 NC_009009:SSA_1256|5end_NAD-dependent_deacetylase,_putative_1283094..1283324_NEGATIVE 181 100 54 147 95 NC_009009:SSA_0671|5end_Methylenetetrahydrofolate_dehydrogenase/methenyltetrahydrofolate_cyclohydrolase,_putative_654340..654570_POSITIVE 180 94 58 110 73 NC_009009:SSA_0975|5end_2-isopropylmalate_synthase,_putative_980971..981201_POSITIVE 179 99 77 119 101 NC_009009:SSA_1449|5end_Histidinol-phosphate/aromatic_aminotransferase_and_cobyric_acid_decarboxylase,_putative_1451931..1452161_NEGATIVE 178 97 63 42 3 NC_009009:SSA_1906|5end_Diadenosine_tetraphosphate_(Ap4A)_hydrolase_(histidine_triad_protein,_cell-cycle_regulation),_putative_1900026..1900256_POSITIVE 177 99 52 150 97 NC_009009:SSA_2004|5end_Zinc_metalloprotease_zmpB_precursor,_putative_2004930..2005160_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 228 98 51 109 62 NC_009009:SSA_2175|3end_hypothetical_protein_2173146..2173296_NEGATIVE 225 99 57 115 78 NC_009009:SSA_2107|3end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing],_putative_2109295..2109445_NEGATIVE 203 99 55 109 64 NC_009009:SSA_1990|3end_Zn-porter_lipoprotein,_putative_1985402..1985552_NEGATIVE 199 100 56 135 94 NC_009009:SSA_0998|3end_hypothetical_protein_998758..998908_POSITIVE 192 100 52 84 21 NC_009009:SSA_0528|3end_Conserved_uncharacterized_protein_526010..526160_POSITIVE 186 99 56 85 40 NC_009009:SSA_2194|3end_Nicotinamide_mononucleotide_transporter,_putative_2194848..2194998_NEGATIVE 184 90 43 146 91 NC_009009:SSA_0865|3end_hypothetical_protein_856850..857000_POSITIVE 183 99 53 149 100 NC_009009:SSA_2059|3end_16S_rRNA_uridine-516_pseudouridylate_synthase,_putative_2060494..2060644_NEGATIVE 181 99 53 89 45 NC_009009:SSA_0827|3end_Conserved_uncharacterized_cytosolic_protein,_putative_806952..807102_POSITIVE 176 99 52 112 78 NC_009009:SSA_1496|3end_hypothetical_protein_1502473..1502623_NEGATIVE 175 88 42 53 9 NC_009009:SSA_2384|3end_Acetyltransferase,_GNAT_family,_putative_1465468..1465618_NEGATIVE 174 90 42 110 51 NC_009009:SSA_0125|3end_50S_ribosomal_protein_L30,_putative_117192..117342_POSITIVE 173 98 58 136 96 NC_009009:SSA_1341|3end_Carbamoyl-phosphate_synthase_large_chain,_putative_1356648..1356798_NEGATIVE 173 100 73 38 8 NC_009009:SSA_0773|3end_PTS_enzyme_I,_putative_754416..754566_POSITIVE 173 100 54 68 20 NC_009009:SSA_0666|3end_hypothetical_protein_649681..649831_NEGATIVE 173 99 62 96 55 NC_009009:SSA_0531|3end_Ethanolamine_utilization_protein_eutQ_(cobalamin-dependent_degradation_of_ethanolamine),_putative_527884..528034_POSITIVE 171 95 53 44 1 NC_009009:SSA_0687|3end_hypothetical_protein_671290..671440_POSITIVE 171 94 60 121 83 NC_009009:SSA_0106|3end_30S_ribosomal_protein_S10,_putative_108160..108310_POSITIVE 170 86 42 146 109 NC_009009:SSA_0176|3end_DNA-directed_RNA_polymerase_I,_beta_chain_(140_kDa_subunit),_putative_178444..178594_POSITIVE 169 42 8 87 41 NC_009009:SSA_0986|3end_Choline_transporter,_putative_989081..989231_POSITIVE