Origin IGS: agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 accttaaagacacgttttgctcgaggtggagcctttatcgaaaccagtcaaaataatattgtttatgagcctaacaaattattcttatcatctacacccctcaaagctactagacgagcaaacttattctttccccttgatttcagagct Mask Tandem Repeat Region ================================================ agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Find is-nt database================================================ Query_seq: SSA_0915:SSA_0916|SSA_0915:SSA_0916:hypothetical protein:2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, putative:->->:928075..928224 150 agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0915:SSA_0916|SSA_0915:SSA_0916:hypothetical protein:2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, putative:->->:928075..928224 150 agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0915:SSA_0916|SSA_0915:SSA_0916:hypothetical protein:2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, putative:->->:928075..928224 150 agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: - Start: 3 End: 134 .. E S I L P F S Y T Q E D L L K S P H L H Y S Y N T L S M F L I I K V P K S L P E V E L M ................ Protein_Len: 38 Strand: - Start: 1 End: 114 A R F D L P F F L N A R R T A K L P T S S L F L K N P E Y V I N N Q S T E M .................................... agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_0915:SSA_0916|SSA_0915:SSA_0916:hypothetical protein:2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, putative:->->:928075..928224 150 agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Intra-Species Hit: Count: 1 Min: 1 Max: 150 Len: 150 Subject: NC_009009_SSA_0915_SSA_0916|hypothetical protein:2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase, putative|POSITIVE:POSITIVE|[928075,928224]|150 HSP 1 e-value: 5.0E-80 bit: 297.0 Len: 150 Query Start:1 Query End:150 Subject Strand: POSITIVE Subject Start: 1 Subject End: 150 agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt agctctgaaatcaaggggaaagaataagtttgctcgtctagtagctttgaggggtgtagatgataagaataatttgttaggctcataaacaatattattttgactggtttcgataaaggctccacctcgagcaaaacgtgtctttaaggt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUCUGAAAUCAAGGGGAAAGAAUAAGUUUGCUCGUCUAGUAGCUUUGAGGGGUGUAGAUGAUAAGAAUAAUUUGUUAGGCUCAUAAACAAUAUUAUUUUGACUGGUUUCGAUAAAGGCUCCACCUCGAGCAAAACGUGUCUUUAAGGU ..((((.......)))).(((((.....((((((((....(.((((((......((.((((.(..(((((((((((((.........))))).))))))))...).))))))...)))))).)....)))))))).....)))))..... (-30.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCUUAAAGACACGUUUUGCUCGAGGUGGAGCCUUUAUCGAAACCAGUCAAAAUAAUAUUGUUUAUGAGCCUAACAAAUUAUUCUUAUCAUCUACACCCCUCAAAGCUACUAGACGAGCAAACUUAUUCUUUCCCCUUGAUUUCAGAGCU .............((((((.((((((.((((....................((((((.(((((.........)))))))))))......................(((........)))...........))))))))))...)))))). (-22.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 334 55 12 80 15 NC_009009:SSA_0153|5end_hypothetical_protein_148677..148907_POSITIVE 309 132 97 167 129 NC_009009:SSA_1584|5end_Ring-cleavage_extradiol_dioxygenase,_putative_1587030..1587260_NEGATIVE 305 148 105 127 65 NC_009009:SSA_1087|5end_ABC-type_transporter_(antibiotic_resistance_protein),_ATPase_component,_putative_1104524..1104754_POSITIVE 296 147 101 75 30 NC_009009:SSA_2049|5end_Polyribonucleotide_nucleotidyltransferase,_putative_2052894..2053124_NEGATIVE 285 60 11 56 12 NC_009009:SSA_1084|5end_Conserved_uncharacterized_protein_1101675..1101905_POSITIVE 285 149 104 167 111 NC_009009:SSA_0512|5end_Nicotinate-nucleotide--dimethylbenzimidazole_phosphoribosyltransferase,_putative_510439..510669_POSITIVE 278 60 11 62 7 NC_009009:SSA_1588|5end_Conserved_ABC-type_antimicrobial_permease-like_protein,_putative_1591905..1592135_NEGATIVE 277 59 12 168 115 NC_009009:SSA_2048|5end_Serine_acetyltransferase,_putative_2050601..2050831_NEGATIVE 274 63 32 195 157 NC_009009:SSA_0043|5end_hypothetical_protein_48801..49031_POSITIVE 268 129 89 192 145 NC_009009:SSA_0102|5end_Kinase,_putative_104035..104265_POSITIVE 265 54 11 229 181 NC_009009:SSA_0677|5end_rRNA_methylase,_putative_659466..659696_POSITIVE 264 134 99 193 150 NC_009009:SSA_1968|5end_Ketol-acid_reductoisomerase,_putative_1964681..1964911_NEGATIVE 263 139 91 72 17 NC_009009:SSA_1094|5end_GTP-binding_protein,_putative_1111576..1111806_POSITIVE 262 50 3 176 130 NC_009009:SSA_2359|5end_Glucose_inhibited_division_protein_A,_putative_2362520..2362750_NEGATIVE 262 62 33 46 12 NC_009009:SSA_1740|5end_hypothetical_protein_1727866..1728096_NEGATIVE 261 48 6 145 108 NC_009009:SSA_0877|5end_Phosphoglycolate_phosphatase,_putative_871008..871238_POSITIVE 261 49 1 216 164 NC_009009:SSA_0481|5end_Cobyric_acid_synthase,_putative_481962..482192_POSITIVE 260 47 1 211 164 NC_009009:SSA_0596|5end_hypothetical_protein_586913..587143_POSITIVE 258 49 3 66 13 NC_009009:SSA_1699|5end_hypothetical_protein_1691096..1691326_NEGATIVE 258 59 13 213 168 NC_009009:SSA_1569|5end_ABC_transporter_membrane-spanning_permease,_arginine/histidine_transport,_putative_1571891..1572121_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 305 148 105 130 68 NC_009009:SSA_1086|3end_TRNA_nucleotidyltransferase,_putative_1104607..1104757_POSITIVE 285 60 11 53 9 NC_009009:SSA_1083|3end_Conserved_uncharacterized_protein_1101752..1101902_POSITIVE 278 60 11 60 5 NC_009009:SSA_1589|3end_ABC-type_antimicrobial_peptide_transport_system,_ATPase_component,_putative_1591907..1592057_NEGATIVE 277 59 12 76 23 NC_009009:SSA_2049|3end_Polyribonucleotide_nucleotidyltransferase,_putative_2050693..2050843_NEGATIVE 264 134 99 128 85 NC_009009:SSA_1969|3end_Acetolactate_synthase_small_subunit,_putative_1964746..1964896_NEGATIVE 263 139 91 61 6 NC_009009:SSA_1093|3end_ATP-dependent_Clp_protease_ATP-binding_subunit_clpX,_putative_1111645..1111795_POSITIVE 263 47 2 92 37 NC_009009:SSA_0311|3end_hypothetical_protein_306703..306853_POSITIVE 261 150 108 54 1 NC_009009:SSA_0430|3end_Cationic_amino_acid_transporter,_putative_431248..431398_NEGATIVE 260 56 27 80 53 NC_009009:SSA_0036|3end_Secreted_protein,_possible_function_in_cell-wall_metabolism_(amidase),_putative_42050..42200_NEGATIVE 259 68 21 69 13 NC_009009:SSA_0152|3end_hypothetical_protein_148731..148881_POSITIVE 258 80 31 94 40 NC_009009:SSA_0258|3end_hypothetical_protein_253022..253172_POSITIVE 254 140 94 141 97 NC_009009:SSA_1620|3end_Uridylate_kinase,_putative_1622256..1622406_NEGATIVE 253 140 105 63 11 NC_009009:SSA_2337|3end_hypothetical_protein_2339359..2339509_POSITIVE 253 149 101 109 57 NC_009009:SSA_0686|3end_Fe2+/Zn2+_uptake_regulation_protein,_putative_670079..670229_POSITIVE 253 63 27 138 93 NC_009009:SSA_0050|3end_Dihydroxyacetone_kinase,_phosphatase_domain_(Dak2),_putative_55270..55420_POSITIVE 250 149 102 89 40 NC_009009:SSA_1424|3end_Hydrolase,_putative_1433889..1434039_NEGATIVE 250 141 117 70 37 NC_009009:SSA_1107|3end_Lipid/multidrug/protein-type_ABC_exporter,_ATP_binding/membrane-spanning_protein,_putative_1133784..1133934_POSITIVE 250 141 106 58 23 NC_009009:SSA_0481|3end_Cobyric_acid_synthase,_putative_483541..483691_POSITIVE 249 140 95 67 29 NC_009009:SSA_2140|3end_Ribonuclease_P_protein_component,_putative_2140308..2140458_NEGATIVE 248 147 102 104 63 NC_009009:SSA_1729|3end_ABC_transporter_substrate-binding_protein-branched_chain_amino_acid_transport,_putative_1714539..1714689_NEGATIVE