Origin IGS: agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggcgcttttctttcttttttcatttcaatgcttaatccaattatacataaaagcacgagcagataattctcaatccagcccaccaaattagcttgattggcttgctctagataacccattaaatggtttgtaccaaaacctagtcctcctaatatcagtgttagtagggtcatctgtaggtaaaatcagtcatacttgagcttgactgctaatagcaagggtaaaatggctagattccaaaagccgatttttcgcctaaaaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggagctcctttctacaatttctctaataaactagcgctcaacttcataaactcttcctgcaagcaagtatgcaatgtcaaagcacaaaaccaagcaaaacaacttccttgctcagtacgattgataaagcagctagtttgtctatcttatctattacgatatgaattgttacgaattaaaatttccctaaaaaattgattagtgaagcgtctagtcagagtccagcagaatttggtgctagtcattttaaatagttacacaatcataataatcccgttcactaggacacgaaaaggcgaaaacgaacttttccgccgatgagttagcaaagctgttagctagtccgcctaattacggctagcgtccaatatagggaatcgcttcccattgtatctgctgcaatatctcaataggaatcgcatattactcctcaaacagaactgcaacttacatagtatatcacaaaagtatataaaaagatagaacttgaatataaaatcttttctctttaacactcattttgaaacaaaacgaaaaattgaaaatcacttttaaaatagttgaaattgataagaaaaggaggcgaactatgaataaatttttacgtgctatatttattttgattattctaactatgctgagcgctgcaatcatccaaatttttctaactcaccttttaggcgaaaaatcggcttttggaatctagccattttacccttgctattagcagtcaagctcaagtatgactgattttacctacagatgaccctactaacactgatattaggaggactaggttttggtacaaaccatttaatgggttatctagagcaagccaatcaagctaatttggtgggctggattgagaattatctgctcgtgcttttatgtataattggattaagcattgaaatgaaaaaagaaagaaaagcgcccaagagacttctcctgattagagaaatctcctagtcgctttgggtgtttttattagcaatccgtttcaaaactttagtgagaactaacgcaaaacagctttagctaaagtaatggtatgttgtagcattttatttaattgtttaagttagacaaactggaatttgtcgctct Mask Tandem Repeat Region ================================================ agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggcgcttttctttcttttttcatttcaatgcttaatccaattatacataaaagcacgagcagataattctcaatccagcccaccaaattagcttgattggcttgctctagataacccattaaatggtttgtaccaaaacctagtcctcctaatatcagtgttagtagggtcatctgtaggtaaaatcagtcatacttgagcttgactgctaatagcaagggtaaaatggctagattccaaaagccgatttttcgcctaaaaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Find is-nt database================================================ Query_seq: SSA_0700:SSA_0701|SSA_0700:SSA_0701:hypothetical protein:Cation transporter CorA family, putative:->->:683401..684507 1107 agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggcgcttttctttcttttttcatttcaatgcttaatccaattatacataaaagcacgagcagataattctcaatccagcccaccaaattagcttgattggcttgctctagataacccattaaatggtttgtaccaaaacctagtcctcctaatatcagtgttagtagggtcatctgtaggtaaaatcagtcatacttgagcttgactgctaatagcaagggtaaaatggctagattccaaaagccgatttttcgcctaaaaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0700:SSA_0701|SSA_0700:SSA_0701:hypothetical protein:Cation transporter CorA family, putative:->->:683401..684507 1107 agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggcgcttttctttcttttttcatttcaatgcttaatccaattatacataaaagcacgagcagataattctcaatccagcccaccaaattagcttgattggcttgctctagataacccattaaatggtttgtaccaaaacctagtcctcctaatatcagtgttagtagggtcatctgtaggtaaaatcagtcatacttgagcttgactgctaatagcaagggtaaaatggctagattccaaaagccgatttttcgcctaaaaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0700:SSA_0701|SSA_0700:SSA_0701:hypothetical protein:Cation transporter CorA family, putative:->->:683401..684507 1107 agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggcgcttttctttcttttttcatttcaatgcttaatccaattatacataaaagcacgagcagataattctcaatccagcccaccaaattagcttgattggcttgctctagataacccattaaatggtttgtaccaaaacctagtcctcctaatatcagtgttagtagggtcatctgtaggtaaaatcagtcatacttgagcttgactgctaatagcaagggtaaaatggctagattccaaaagccgatttttcgcctaaaaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 175 Max: 432 Len: 258 Subject: UniRef90_A3CLT6 Cluster: Hypothetical protein; n=1; Streptococcus sanguinis SK36|Rep: Hypothetical protein - Streptococcus sanguinis (strain SK36) HSP 1 e-value: 6.0E-14 bit: 81.3 Len: 258 Query Start:175 Query End:432 Subject Strand: null Subject Start: 42 Subject End: 127 .............................................................................................................................................................................. F R R K I G F W N X X X X X X X X X X X X K Y D * F Y L Q M X X X X X X X X X X X X X T N H L M G Y L E Q A N Q A N L V G W I E N Y L L V L L C I I G L S I E M K K E R K A ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... .............................................................................................................................................................................. W Q R E I G F W N L A I L P L V I A A N M K Y D W F Y L R M T L L A L I L G G L G F G T N H L L G Y L E K A N Q A N L L G W I E N Y L L V F C W I I G W G L E Y R K R Q K S ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 146 End: 235 ................................................................................................................................................. M S L I R R S L L G A F L S F F I S M L N P I I H K S T S R ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 60 Strand: + Start: 448 End: 645 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................... M W M I A A L S I V R I I K I N I A R K N L F I V R L L F L S I S T I L K V I F N F S F C F K M S V K E K R F Y I Q V L .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 55 Strand: + Start: 593 End: 757 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L K R K D F I F K F Y L F I Y F C D I L C K L Q F C L R S N M R F L L R Y C S R Y N G K R F P I L D A S R N .............................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 32 Strand: + Start: 863 End: 958 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M T S T K F C W T L T R R F T N Q F F R E I L I R N N S Y R N R ..................................................................................................................................................... Protein_Len: 37 Strand: + Start: 983 End: 1093 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M N R T E Q G S C F A W F C A L T L H T C L Q E E F M K L S A S L L E K L .............. Protein_Len: 35 Strand: - Start: 947 End: 1051 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. I T I S L I S L S A A K D I T S L L S T T K S P K T S Q C Q M S A Q M ........................................................ Protein_Len: 50 Strand: - Start: 913 End: 1062 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ N K L S I K I R L L E Y R L L Y S L C V L Q K I L R V S C P L Q K A Q N Q A K V N C V Q K C S S N M ............................................. Protein_Len: 39 Strand: - Start: 790 End: 906 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... R R F L E N E G K R T R T F P I I I I T Y S N L I V L V L N Q Q V R V L R K M ......................................................................................................................................................................................................... Protein_Len: 32 Strand: - Start: 636 End: 731 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I S K H Y V I Y T A T R N S S Y Y A I G I S I N C C I C H S A M ........................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 37 Strand: - Start: 628 End: 738 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... R K I Y K Q S I S H L N C N Q K L L L I R N R N L Y Q L L Y L P F R N G M ................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 405 End: 590 .................................................................................................................................................................................................................................................................................................................................................................................................................... F A S K E G L L H T L F I Q I I A A S L M T L I I L I F I A R L F K N M T R R R K K D I E V I K F T I K L K E N Q K L M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 50 Strand: - Start: 361 End: 510 ........................................................................................................................................................................................................................................................................................................................................................................ D Y K L K V A L L L P L I A L N W F G I K R R F P S N S F N P H N C R E A Y N S Y D F Y I Y C T F M ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: - Start: 263 End: 361 ...................................................................................................................................................................................................................................................................... S S Q S A R S L G N F P K Y W F R T R R I D T N T P D D T P L I M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 142 End: 345 ............................................................................................................................................. R K P A K R E K K M E I S L G I I C L L V L L Y N E I W G V L N A Q N A Q E L Y G M L H N T G F G L G G L I L T L L T M .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 45 HP_score: -2.1 Tail_Score: -3.37071 Start: 70 End: 89 Full_Region: ATACCATTACTTTAG CTAAAGC TGTTTT GCGTTAG TTCTCACTAAAGTTT .....................................................................CTAAAGCTGTTTTGCGTTAG.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 59 HP_score: -4.8 Tail_Score: -3.89368 Start: 135 End: 177 Full_Region: aaaaaagaaagaaaa gcgcccaagagacttctc ctgatta gagaaatctcctagtcgc tttgggtgtttttat ......................................................................................................................................gcgcccaagagacttctcctgattagagaaatctcctagtcgc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -12.1 Tail_Score: -5.3472 Start: 135 End: 177 Full_Region: ATAAAAACACCCAAA GCGACTAGGAGATTTCTCT AATCA GGAGAAGTCTCTTGGGCGC TTTTCTTTCTTTTTT ......................................................................................................................................GCGACTAGGAGATTTCTCTAATCAGGAGAAGTCTCTTGGGCGC.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 74 HP_score: -6.6 Tail_Score: -4.37502 Start: 792 End: 813 Full_Region: CTTTGCTAACTCATC GGCGGAAA AGTTCG TTTTCGCC TTTTCGTGTCCTAGT .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGCGGAAAAGTTCGTTTTCGCC...................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: SSA_0700:SSA_0701|SSA_0700:SSA_0701:hypothetical protein:Cation transporter CorA family, putative:->->:683401..684507 1107 agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctcttgggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctccttttcttatcaatttcaactattttaaaagtgattttcaatttttcgttttgtttcaaaatgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc Intra-Species Hit: Count: 1 Min: 1 Max: 1107 Len: 1107 Subject: NC_009009_SSA_0700_SSA_0701|hypothetical protein:Cation transporter CorA family, putative|POSITIVE:POSITIVE|[683401,684507]|1107 HSP 1 e-value: 0.0 bit: 1027.0 Len: 518 Query Start:590 Query End:1107 Subject Strand: POSITIVE Subject Start: 590 Subject End: 1107 .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagtgttaaagagaaaagattttatattcaagttctatctttttatatacttttgtgatatactatgtaagttgcagttctgtttgaggagtaatatgcgattcctattgagatattgcagcagatacaatgggaagcgattccctatattggacgctagccgtaattaggcggactagctaacagctttgctaactcatcggcggaaaagttcgttttcgccttttcgtgtcctagtgaacgggattattatgattgtgtaactatttaaaatgactagcaccaaattctgctggactctgactagacgcttcactaatcaattttttagggaaattttaattcgtaacaattcatatcgtaatagataagatagacaaactagctgctttatcaatcgtactgagcaaggaagttgttttgcttggttttgtgctttgacattgcatacttgcttgcaggaagagtttatgaagttgagcgctagtttattagagaaattgtagaaaggagctcc HSP 2 e-value: 1.0E-91 bit: 339.0 Len: 171 Query Start:1 Query End:171 Subject Strand: POSITIVE Subject Start: 1 Subject End: 171 agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctctt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ agagcgacaaattccagtttgtctaacttaaacaattaaataaaatgctacaacataccattactttagctaaagctgttttgcgttagttctcactaaagttttgaaacggattgctaataaaaacacccaaagcgactaggagatttctctaatcaggagaagtctctt........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ HSP 3 e-value: 2.0E-47 bit: 192.0 Len: 97 Query Start:433 Query End:529 Subject Strand: POSITIVE Subject Start: 433 Subject End: 529 ................................................................................................................................................................................................................................................................................................................................................................................................................................................aggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctcc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ................................................................................................................................................................................................................................................................................................................................................................................................................................................aggtgagttagaaaaatttggatgattgcagcgctcagcatagttagaataatcaaaataaatatagcacgtaaaaatttattcatagttcgcctcc.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGCGACAAAUUCCAGUUUGUCUAACUUAAACAAUUAAAUAAAAUGCUACAACAUACCAUUACUUUAGCUAAAGCUGUUUUGCGUUAGUUCUCACUAAAGUUUUGAAACGGAUUGCUAAUAAAAACACCCAAAGCGACUAGGAGAUUUCUCUAAUCAGGAGAAGUCUCUUGGGCGCUUUUCUUUCUUUUUUCAUUUCAAUGCUUAAUCCAAUUAUACAUAAAAGCACGAGCAGAUAAUUCUCAAUCCAGCCCACCAAAUUAGCUUGAUUGGCUUGCUCUAGAUAACCCAUUAAAUGGUUUGUACCAAAACCUAGUCCUCCUAAUAUCAGUGUUAGUAGGGUCAUCUGUAGGUAAAAUCAGUCAUACUUGAGCUUGACUGCUAAUAGCAAGGGUAAAAUGGCUAGAUUCCAAAAGCCGAUUUUUCGCCUAAAAGGUGAGUUAGAAAAAUUUGGAUGAUUGCAGCGCUCAGCAUAGUUAGAAUAAUCAAAAUAAAUAUAGCACGUAAAAAUUUAUUCAUAGUUCGCCUCCUUUUCUUAUCAAUUUCAACUAUUUUAAAAGUGAUUUUCAAUUUUUCGUUUUGUUUCAAAAUGAGUGUUAAAGAGAAAAGAUUUUAUAUUCAAGUUCUAUCUUUUUAUAUACUUUUGUGAUAUACUAUGUAAGUUGCAGUUCUGUUUGAGGAGUAAUAUGCGAUUCCUAUUGAGAUAUUGCAGCAGAUACAAUGGGAAGCGAUUCCCUAUAUUGGACGCUAGCCGUAAUUAGGCGGACUAGCUAACAGCUUUGCUAACUCAUCGGCGGAAAAGUUCGUUUUCGCCUUUUCGUGUCCUAGUGAACGGGAUUAUUAUGAUUGUGUAACUAUUUAAAAUGACUAGCACCAAAUUCUGCUGGACUCUGACUAGACGCUUCACUAAUCAAUUUUUUAGGGAAAUUUUAAUUCGUAACAAUUCAUAUCGUAAUAGAUAAGAUAGACAAACUAGCUGCUUUAUCAAUCGUACUGAGCAAGGAAGUUGUUUUGCUUGGUUUUGUGCUUUGACAUUGCAUACUUGCUUGCAGGAAGAGUUUAUGAAGUUGAGCGCUAGUUUAUUAGAGAAAUUGUAGAAAGGAGCUCC .((((..(......((((((.(((((..(((((............(((..........((..(((((((((((.((......)).))))......)))))))..))....((((((..(((..........((((((.(((((((((((((((.....))))))))))))))).))))))....................(((((.......................)))))....)))..))))))....(((((((((.....(((((((((....(((((.((((.......(((((......)))))........((((((....))))))..)))).)))))..((...((((((((((..(((..((((.(((....)))))))..))).)))))).))))))..))))))))).((((((.....)))))).......))))))).))...)))(((((((((.((((((((..........((((((..(((((...........(((((((((((.((((.....((((.........((((.((((((((...........(((((((((...)))))))))...((((((((.(..((((.......))))..).))))))))..)))))))).))))..........(((((((((((.....((((((........))))))....))).)))))))).))))....)))).)))).((((..(((((((((((((((((......)))).))))).....................((((((((......)))))))).......)))).))))...))))..))))))).)))))...))))))......((((((........))))))..)))))))).....(((.((((........)))).))).......((((((((..(((.....(((...(((..((((((((.......)).)))))).))).)))...(((((.(((((((..(((..(((.....)))..).))..))).)))).)))))..)))..).))))))))))))))))..)))))))))).))))))......)..)))). (-284.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGAGCUCCUUUCUACAAUUUCUCUAAUAAACUAGCGCUCAACUUCAUAAACUCUUCCUGCAAGCAAGUAUGCAAUGUCAAAGCACAAAACCAAGCAAAACAACUUCCUUGCUCAGUACGAUUGAUAAAGCAGCUAGUUUGUCUAUCUUAUCUAUUACGAUAUGAAUUGUUACGAAUUAAAAUUUCCCUAAAAAAUUGAUUAGUGAAGCGUCUAGUCAGAGUCCAGCAGAAUUUGGUGCUAGUCAUUUUAAAUAGUUACACAAUCAUAAUAAUCCCGUUCACUAGGACACGAAAAGGCGAAAACGAACUUUUCCGCCGAUGAGUUAGCAAAGCUGUUAGCUAGUCCGCCUAAUUACGGCUAGCGUCCAAUAUAGGGAAUCGCUUCCCAUUGUAUCUGCUGCAAUAUCUCAAUAGGAAUCGCAUAUUACUCCUCAAACAGAACUGCAACUUACAUAGUAUAUCACAAAAGUAUAUAAAAAGAUAGAACUUGAAUAUAAAAUCUUUUCUCUUUAACACUCAUUUUGAAACAAAACGAAAAAUUGAAAAUCACUUUUAAAAUAGUUGAAAUUGAUAAGAAAAGGAGGCGAACUAUGAAUAAAUUUUUACGUGCUAUAUUUAUUUUGAUUAUUCUAACUAUGCUGAGCGCUGCAAUCAUCCAAAUUUUUCUAACUCACCUUUUAGGCGAAAAAUCGGCUUUUGGAAUCUAGCCAUUUUACCCUUGCUAUUAGCAGUCAAGCUCAAGUAUGACUGAUUUUACCUACAGAUGACCCUACUAACACUGAUAUUAGGAGGACUAGGUUUUGGUACAAACCAUUUAAUGGGUUAUCUAGAGCAAGCCAAUCAAGCUAAUUUGGUGGGCUGGAUUGAGAAUUAUCUGCUCGUGCUUUUAUGUAUAAUUGGAUUAAGCAUUGAAAUGAAAAAAGAAAGAAAAGCGCCCAAGAGACUUCUCCUGAUUAGAGAAAUCUCCUAGUCGCUUUGGGUGUUUUUAUUAGCAAUCCGUUUCAAAACUUUAGUGAGAACUAACGCAAAACAGCUUUAGCUAAAGUAAUGGUAUGUUGUAGCAUUUUAUUUAAUUGUUUAAGUUAGACAAACUGGAAUUUGUCGCUCU (((((..(.(((((.............(((((((((((.....(((......((....(((((.((((.(((.....................))).....)))).)))))..))......)))...))).))))))))(((((.((((......((((((((((.((((((((...........((((......(((((((........)))))))(((..(((((((..((((((((((((........((((.((.................))..))))((((.......((((((((.....)))).))))....((((((((....)))))))).))))..........)))))))).))))....(((((....)))))......)))))))......)))..))))....((.(((((((........................((((...((((.(((((...((((.(((.(((..(((....)))..)))..))).))))........((((((((...((((((((((....((((.((((((.....)))))).)))).........((.....)).......))))))).)))((((..(((((((..((((.(((((.(((((.((((((.(((..........((..((((.((((((.....(((..(((((.((((..........)))))))))...((((((.....((((((.((....)).))))))...........(((((((((..((((........))))........(((((......))))).......)))))))))..))))))........))).....)))))).))))..))........)))..))))))....))))).))))).))...))..))))))).........(((((((((((((((....)))..((((((((....)).))))))...))))))))))))..)))).....))))))))))))).))))..)))).........(((....)))..)))))))))...)))))))).)))))...))))).)))).)))))....)))))...)..))))) (-261.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 412 788 742 227 167 NC_009009:SSA_0990|5end_hypothetical_protein_991733..991963_POSITIVE 376 480 434 54 11 NC_009009:SSA_0705|5end_hypothetical_protein_690950..691180_NEGATIVE 374 278 231 53 4 NC_009009:SSA_2186|5end_Glutathione_biosynthesis_bifunctional_protein_gshAB_(Gamma-GCS-GS)_(GCS-GS),_putative_2185535..2185765_POSITIVE 364 820 775 69 17 NC_009009:SSA_2089|5end_L-xylulose-5-phosphate_3-epimerase,_putative_2094339..2094569_NEGATIVE 356 179 131 223 166 NC_009009:SSA_2122|5end_hypothetical_protein_2127607..2127837_NEGATIVE 352 282 248 64 33 NC_009009:SSA_0272|5end_Oxidoreductase,_aldo/keto_reductase_family,_putative_266023..266253_POSITIVE 349 179 138 210 162 NC_009009:SSA_0316|5end_hypothetical_protein_310576..310806_POSITIVE 347 350 305 190 143 NC_009009:SSA_2278|5end_Ukp_protein,_putative_2281669..2281899_NEGATIVE 346 210 163 221 168 NC_009009:SSA_0998|5end_hypothetical_protein_998487..998717_POSITIVE 344 274 233 155 116 NC_009009:SSA_1764|5end_hypothetical_protein_1750868..1751098_POSITIVE 336 183 142 45 1 NC_009009:SSA_2294|5end_hypothetical_protein_2296082..2296312_POSITIVE 336 911 873 66 25 NC_009009:SSA_1821|5end_Acetyltransferase_(N-acetylase_of_ribosomal_proteins),_putative_1810610..1810840_NEGATIVE 333 824 782 59 7 NC_009009:SSA_0452|5end_Nitrogen_utilization_substance_protein_B,_putative_451534..451764_POSITIVE 329 777 731 77 39 NC_009009:SSA_1418|5end_Conserved_uncharacterized_protein_1428927..1429157_NEGATIVE 329 429 386 140 103 NC_009009:SSA_0490|5end_Cobalamin_5'-phosphate_synthase,_putative_490236..490466_POSITIVE 327 280 247 35 1 NC_009009:SSA_0677|5end_rRNA_methylase,_putative_659466..659696_POSITIVE 319 840 791 212 168 NC_009009:SSA_1782|5end_hypothetical_protein_1768123..1768353_NEGATIVE 319 779 733 225 171 NC_009009:SSA_0348|5end_CAAX_amino_terminal_protease_family,_putative_340288..340518_POSITIVE 317 188 149 136 99 NC_009009:SSA_1910|5end_hypothetical_protein_1902672..1902902_NEGATIVE 315 366 323 53 6 NC_009009:SSA_0783|5end_Proton-translocating_ATPase,_F0_sector,_subunit_a,_putative_766815..767045_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 364 820 775 65 13 NC_009009:SSA_2090|3end_Hexulose-6-phosphate_synthase,_putative_2094343..2094493_NEGATIVE 352 282 248 54 23 NC_009009:SSA_0271|3end_Glycosylhydrolase,_family_35,_putative_266093..266243_POSITIVE 345 280 243 42 2 NC_009009:SSA_0676|3end_Farnesyl_diphosphate_synthase,_putative_659553..659703_POSITIVE 333 824 782 81 29 NC_009009:SSA_0451|3end_Conserved_uncharacterized_protein_451636..451786_POSITIVE 329 429 386 143 106 NC_009009:SSA_0489|3end_Adenosylcobinamide_kinase_/_adenosylcobinamide_phosphate_guanyltransferase,_putative_490319..490469_POSITIVE 317 188 149 143 106 NC_009009:SSA_1911|3end_Conserved_uncharacterized_protein_1902665..1902815_NEGATIVE 315 825 784 56 7 NC_009009:SSA_0546|3end_Phospho-2-dehydro-3-deoxyheptonate_aldolase_(DAHP_synthatase),_possibly_tyr-sensitive,_putative_541963..542113_POSITIVE 313 1025 981 63 22 NC_009009:SSA_0660|3end_Cell_division_protein_DivIVA,_putative_644672..644822_POSITIVE 310 1077 1032 39 2 NC_009009:SSA_2081|3end_hypothetical_protein_2085490..2085640_POSITIVE 305 1078 1031 51 6 NC_009009:SSA_1258|3end_Purine_nucleoside_phosphorylase,_family_1,_putative_1283858..1284008_NEGATIVE 301 826 781 47 3 NC_009009:SSA_1076|3end_hypothetical_protein_1095047..1095197_POSITIVE 300 278 232 141 92 NC_009009:SSA_0621|3end_SOS_responce_UmuC_protein,_putative_612322..612472_NEGATIVE 300 430 381 122 72 NC_009009:SSA_0149|3end_hypothetical_protein_143833..143983_POSITIVE 299 290 246 60 23 NC_009009:SSA_0025|3end_DNA_repair_protein_recO,_putative_30547..30697_POSITIVE 298 906 880 35 6 NC_009009:SSA_2184|3end_hypothetical_protein_2183284..2183434_NEGATIVE 297 796 752 146 97 NC_009009:SSA_1102|3end_hypothetical_protein_1123807..1123957_POSITIVE 296 817 774 109 66 NC_009009:SSA_1304|3end_hypothetical_protein_1323087..1323237_NEGATIVE 293 378 334 81 43 NC_009009:SSA_1799|3end_Acetyltransferase,_putative_1780513..1780663_NEGATIVE 293 354 311 82 33 NC_009009:SSA_1409|3end_DTDP-glucose-4,6-dehydratase,_putative_1421209..1421359_NEGATIVE 293 286 248 75 44 NC_009009:SSA_0469|3end_Precorrin-6B_methylase_2,putative_472959..473109_POSITIVE