Origin IGS: attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tacaccagtcctctctatcttccatgtttccggtctatactaataacgctaaaaattagagttacccaaactttattataacataaagaaactgaaaatgcgcagtttttctacatataat Mask Tandem Repeat Region ================================================ attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Find is-nt database================================================ Query_seq: SSA_0654:SSA_0655|SSA_0654:SSA_0655:Cell division protein DivIB, putative:Cell division protein FtsA, putative:->->:638803..638923 121 attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0654:SSA_0655|SSA_0654:SSA_0655:Cell division protein DivIB, putative:Cell division protein FtsA, putative:->->:638803..638923 121 attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0654:SSA_0655|SSA_0654:SSA_0655:Cell division protein DivIB, putative:Cell division protein FtsA, putative:->->:638803..638923 121 attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Predict ORF larger than 30AA ================================================ attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 43 HP_score: -2.5 Tail_Score: -3.32844 Start: 47 End: 65 Full_Region: aataacgctaaaaat tagagtt accca aacttta ttataacataaagaa ..............................................tagagttacccaaacttta........................................................ Find igs database================================================ Query_seq: SSA_0654:SSA_0655|SSA_0654:SSA_0655:Cell division protein DivIB, putative:Cell division protein FtsA, putative:->->:638803..638923 121 attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Intra-Species Hit: Count: 1 Min: 1 Max: 121 Len: 121 Subject: NC_009009_SSA_0654_SSA_0655|Cell division protein DivIB, putative:Cell division protein FtsA, putative|POSITIVE:POSITIVE|[638803,638923]|121 HSP 1 e-value: 8.0E-63 bit: 240.0 Len: 121 Query Start:1 Query End:121 Subject Strand: POSITIVE Subject Start: 1 Subject End: 121 attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta attatatgtagaaaaactgcgcattttcagtttctttatgttataataaagtttgggtaactctaatttttagcgttattagtatagaccggaaacatggaagatagagaggactggtgta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUAUAUGUAGAAAAACUGCGCAUUUUCAGUUUCUUUAUGUUAUAAUAAAGUUUGGGUAACUCUAAUUUUUAGCGUUAUUAGUAUAGACCGGAAACAUGGAAGAUAGAGAGGACUGGUGUA .......((((.....))))......(((((((((((((.((........(((((((((((.(((.....))).))))))....))))).(....)....)).))))))).)))))).... (-22.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACACCAGUCCUCUCUAUCUUCCAUGUUUCCGGUCUAUACUAAUAACGCUAAAAAUUAGAGUUACCCAAACUUUAUUAUAACAUAAAGAAACUGAAAAUGCGCAGUUUUUCUACAUAUAAU .......((...(((((.......((((...(((....)))...))))........)))))..)).........((((((...((.((((((((........)))))))).))..)))))) (-12.26) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 299 120 82 230 194 NC_009009:SSA_0765|5end_hypothetical_protein_745519..745749_POSITIVE 285 106 61 148 102 NC_009009:SSA_1530|5end_ABC-type_antimicrobial_peptide_transport_system,_permease_component,_putative_1538778..1539008_NEGATIVE 262 118 74 76 29 NC_009009:SSA_1600|5end_hypothetical_protein_1606595..1606825_NEGATIVE 260 70 21 84 36 NC_009009:SSA_0938|5end_hypothetical_protein_946587..946817_POSITIVE 259 111 80 169 134 NC_009009:SSA_0587|5end_Metal-dependent_amidase/aminoacylase/carboxypeptidase,_putative_578539..578769_POSITIVE 256 116 72 214 171 NC_009009:SSA_0141|5end_Copper_chaperone,_putative_136893..137123_POSITIVE 254 118 74 87 40 NC_009009:SSA_0845|5end_ABC-type_multidrug_transport_system,_ATPase_component,_putative_830046..830276_NEGATIVE 242 120 71 173 128 NC_009009:SSA_0929|5end_ABC-type_multidrug_transporter,_ATPase_and_permease_components,_putative_938438..938668_POSITIVE 241 120 86 218 193 NC_009009:SSA_0763|5end_Transcription_repressor,_putative_744980..745210_POSITIVE 236 119 72 126 83 NC_009009:SSA_1132|5end_TatC,_sec-independent_protein_translocase,_putative_1158645..1158875_POSITIVE 234 116 72 177 132 NC_009009:SSA_0607|5end_ABC_transporter,_permease_component,_putative_596926..597156_NEGATIVE 233 120 72 219 170 NC_009009:SSA_2083|5end_hypothetical_protein_2087969..2088199_NEGATIVE 231 118 74 191 143 NC_009009:SSA_1596|5end_hypothetical_protein_1603034..1603264_NEGATIVE 231 118 74 65 5 NC_009009:SSA_0167|5end_Hypothetical_protein_(Asparagine/proline-rich)_165254..165484_POSITIVE 224 118 71 57 20 NC_009009:SSA_1569|5end_ABC_transporter_membrane-spanning_permease,_arginine/histidine_transport,_putative_1571891..1572121_POSITIVE 224 116 73 54 15 NC_009009:SSA_0260|5end_Manganese/Zinc_ABC_transporter_substrate-binding_lipoprotein_precursor,_putative_254585..254815_NEGATIVE 223 120 72 209 155 NC_009009:SSA_1360|5end_Arginine/histidine_ABC_transporter,_ATPase_component,_putative_1376350..1376580_POSITIVE 223 40 6 47 15 NC_009009:SSA_0406|5end_hypothetical_protein_402918..403148_NEGATIVE 222 118 80 174 135 NC_009009:SSA_0861|5end_hypothetical_protein_852977..853207_NEGATIVE 220 41 9 170 132 NC_009009:SSA_2346|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III,_putative_2348546..2348776_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 285 106 61 148 102 NC_009009:SSA_1531|3end_ABC-type_antimicrobial_peptide_transporter,_ATPase_component,_putative_1538778..1538928_NEGATIVE 262 118 74 79 32 NC_009009:SSA_1601|3end_VacB-like_protein,_putative_1606592..1606742_NEGATIVE 260 70 21 81 33 NC_009009:SSA_0937|3end_Arsenate_reductase,_glutaredoxin_family,_putative_946664..946814_POSITIVE 237 117 75 94 47 NC_009009:SSA_0436|3end_Imidazolonepropionase,_putative_440214..440364_POSITIVE 228 119 75 73 22 NC_009009:SSA_0422|3end_Phosphoglycerate_mutase_family_protein,_putative_420839..420989_POSITIVE 224 118 71 46 9 NC_009009:SSA_1568|3end_ABC_transporter_membrane-spanning_permease,_arginine/histidine_transport,_putative_1571960..1572110_POSITIVE 223 40 6 54 22 NC_009009:SSA_0407|3end_ABC-type_multidrug_transport_system_(3-component_subtilin_immunity_exporter),_ATPase_component,_putative_402911..403061_NEGATIVE 220 41 9 69 31 NC_009009:SSA_2347|3end_Coenzyme_F390_synthetase,_putative_2348647..2348797_NEGATIVE 220 117 84 66 29 NC_009009:SSA_0842|3end_Effector_of_murein_hydrolase_LrgA/holin-like_protein,_putative_827790..827940_POSITIVE 220 110 68 49 13 NC_009009:SSA_0133|3end_50S_ribosomal_protein_L17,_putative_122562..122712_POSITIVE 219 116 72 52 19 NC_009009:SSA_0999|3end_Biotin--[acetyl-CoA-carboxylase]_ligase,_putative_998781..998931_NEGATIVE 218 90 54 103 71 NC_009009:SSA_0263|3end_Zinc_metalloproteinase_in_scaA_5'region,_putative_258246..258396_POSITIVE 214 52 17 99 55 NC_009009:SSA_1808|3end_hypothetical_protein_1791394..1791544_NEGATIVE 214 119 74 45 6 NC_009009:SSA_0496|3end_Succinylglutamate_desuccinylase_/_Aspartoacylase_family,_putative_497009..497159_POSITIVE 210 119 77 64 20 NC_009009:SSA_0336|3end_Isopentenyl-diphosphate_delta-isomerase,_putative_330879..331029_POSITIVE 209 117 72 55 11 NC_009009:SSA_1621|3end_Amino_acid_transporter,_putative_1623172..1623322_NEGATIVE 208 42 2 88 39 NC_009009:SSA_0804|3end_Phosphoglucomutase/phosphomannomutase_family_protein,_putative_787427..787577_POSITIVE 208 117 86 56 10 NC_009009:SSA_0066|3end_hypothetical_protein_68095..68245_POSITIVE 207 116 87 34 5 NC_009009:SSA_0261|3end_ABC-type_Mn2+/Zn2+_transport_systems,_permease_component,_putative_254605..254755_NEGATIVE 207 76 39 150 115 NC_009009:SSA_0193|3end_CAAX_amino_terminal_protease_family_protein,_putative_195075..195225_POSITIVE