Origin IGS: tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aagacagctcctttcgctgacatatattttgaagcgcttacaattttagggaataaggaaatcagataagaggagcaccatagctatacgcctctttctgattcctttttttaactttttttgggtcaaaagaatagattattctaaggttttctgttaaaatgagtatggttgttttttaaaaaggaaaaaattctgcattctcaaaaagagcctaaccaacttttttagaatgccaagctaagcctacaagaaatatacttgacaggcctttcccttcaaaaataacaatcacagagacagataacagatgataaacttttcgcagggttgctcatcttctatctgtttttttattttccatcattacagaatggcaggacagcaggccgctgtcctgccattctagtaaatctgatagttcaaatcttccgacaaa Mask Tandem Repeat Region ================================================ tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Find is-nt database================================================ Query_seq: SSA_0531:SSA_0532|SSA_0531:SSA_0532:Ethanolamine utilization protein eutQ (cobalamin-dependent degradation of ethanolamine), putative:Propanediol utilization protein PduB, putative:->->:527945..528383 439 tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0531:SSA_0532|SSA_0531:SSA_0532:Ethanolamine utilization protein eutQ (cobalamin-dependent degradation of ethanolamine), putative:Propanediol utilization protein PduB, putative:->->:527945..528383 439 tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0531:SSA_0532|SSA_0531:SSA_0532:Ethanolamine utilization protein eutQ (cobalamin-dependent degradation of ethanolamine), putative:Propanediol utilization protein PduB, putative:->->:527945..528383 439 tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 112 End: 222 ............................................................................................................... M R K V Y H L L S V S V I V I F E G K G L S S I F L V G L A W H S K K V G ......................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 271 End: 375 .............................................................................................................................................................................................................................................................................. M L I L T E N L R I I Y S F D P K K V K K R N Q K E A Y S Y G A P L I ................................................................ Protein_Len: 35 Strand: - Start: 283 End: 387 .......................................................................................................................................................................................................................................................................................... C F V K S Y D I R K V W F F N F F P I L F L R I A I T S R K D S K R M .................................................... Protein_Len: 32 Strand: - Start: 34 End: 129 ................................. F P L V A A Q Q G A M R Y H H F I F F C I S S S C G Q S F N I M ...................................................................................................................................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 2 End: 136 . Q R F I Q V I L N V L I A P C R G A T R G N Q L S P F Y F F L Y F I L L G A F L K D D T M ............................................................................................................................................................................................................................................................................................................... tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 277 End: 306 ....................................................................................................................................................................................................................................................................................ATAGATTATTCTAAGGTTTTCTGTTAAAAT..................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -19.5 Tail_Score: -2.86343 Start: 27 End: 74 Full_Region: tttttattttccatc attacagaatggcaggacagc aggcc gctgtcctgccattctagtaaa tctgatagttcaaat ..........................attacagaatggcaggacagcaggccgctgtcctgccattctagtaaa............................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -23.8 Tail_Score: -2.82516 Start: 34 End: 68 Full_Region: CTATCAGATTTACTA GAATGGCAGGACAGCG GCC TGCTGTCCTGCCATTC TGTAATGATGGAAAA .................................GAATGGCAGGACAGCGGCCTGCTGTCCTGCCATTC................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -19.7 Tail_Score: -3.56273 Start: 37 End: 65 Full_Region: TCAGATTTACTAGAA TGGCAGGACAGCG GCC TGCTGTCCTGCCA TTCTGTAATGATGGA ....................................TGGCAGGACAGCGGCCTGCTGTCCTGCCA...................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -19.0 Tail_Score: -3.43492 Start: 37 End: 65 Full_Region: tccatcattacagaa tggcaggacagc aggcc gctgtcctgcca ttctagtaaatctga ....................................tggcaggacagcaggccgctgtcctgcca...................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 91 HP_score: -2.8 Tail_Score: -6.09196 Start: 92 End: 139 Full_Region: taacaatcacagaga cagataacagatgataaacttt tcgc agggttgctcatcttctatctg tttttttattttcca ...........................................................................................cagataacagatgataaacttttcgcagggttgctcatcttctatctg............................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 56 HP_score: -5.8 Tail_Score: -2.80522 Start: 335 End: 382 Full_Region: AAAAAGTTAAAAAAA GGAATCAGAAAGAGGCGTAT AGCTATG GTGCTCCTCTTATCTGATTTC CTTATTCCCTAAAAT ..............................................................................................................................................................................................................................................................................................................................................GGAATCAGAAAGAGGCGTATAGCTATGGTGCTCCTCTTATCTGATTTC......................................................... TransTerm Strand: + Conf: 50 HP_score: -3.4 Tail_Score: -3.68341 Start: 335 End: 383 Full_Region: AAAAAGTTAAAAAAA GGAATCAGAAAGAGGCGTAT AGCTATG GTGCTCCTCTTATCTGATTTCC TTATTCCCTAAAATT ..............................................................................................................................................................................................................................................................................................................................................GGAATCAGAAAGAGGCGTATAGCTATGGTGCTCCTCTTATCTGATTTCC........................................................ TransTerm Strand: + Conf: 57 HP_score: -3.0 Tail_Score: -4.08689 Start: 339 End: 378 Full_Region: AGTTAAAAAAAGGAA TCAGAAAGAGGCGTAT AGCTATG GTGCTCCTCTTATCTGA TTTCCTTATTCCCTA ..................................................................................................................................................................................................................................................................................................................................................TCAGAAAGAGGCGTATAGCTATGGTGCTCCTCTTATCTGA............................................................. Find igs database================================================ Query_seq: SSA_0531:SSA_0532|SSA_0531:SSA_0532:Ethanolamine utilization protein eutQ (cobalamin-dependent degradation of ethanolamine), putative:Propanediol utilization protein PduB, putative:->->:527945..528383 439 tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaattttttcctttttaaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgacccaaaaaaagttaaaaaaaggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt Intra-Species Hit: Count: 1 Min: 1 Max: 439 Len: 439 Subject: NC_009009_SSA_0531_SSA_0532|Ethanolamine utilization protein eutQ (cobalamin-dependent degradation of ethanolamine), putative:Propanediol utilization protein PduB, putative|POSITIVE:POSITIVE|[527945,528383]|439 HSP 1 e-value: 1.0E-124 bit: 446.0 Len: 246 Query Start:1 Query End:246 Subject Strand: POSITIVE Subject Start: 1 Subject End: 246 tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaatnnnnnnncagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaa................................................................................................................................................................................................. tttgtcggaagatttgaactatcagatttactagaatggcaggacagcggcctgctgtcctgccattctgtaatgatggaaaataaaaaaacagatagaagatgagcaaccctgcgaaaagtttatcatctgttatctgtctctgtgattgttatttttgaagggaaaggcctgtcaagtatatttcttgtaggcttagcttggcattctaaaaaagttggttaggctctttttgagaatgcagaa................................................................................................................................................................................................. HSP 2 e-value: 1.0E-52 bit: 208.0 Len: 105 Query Start:335 Query End:439 Subject Strand: POSITIVE Subject Start: 335 Subject End: 439 ..............................................................................................................................................................................................................................................................................................................................................ggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt ..............................................................................................................................................................................................................................................................................................................................................ggaatcagaaagaggcgtatagctatggtgctcctcttatctgatttccttattccctaaaattgtaagcgcttcaaaatatatgtcagcgaaaggagctgtctt HSP 3 e-value: 1.0E-24 bit: 115.0 Len: 58 Query Start:260 Query End:317 Subject Strand: POSITIVE Subject Start: 260 Subject End: 317 ...................................................................................................................................................................................................................................................................aaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgaccc.......................................................................................................................... ...................................................................................................................................................................................................................................................................aaaaaacaaccatactcattttaacagaaaaccttagaataatctattcttttgaccc.......................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUGUCGGAAGAUUUGAACUAUCAGAUUUACUAGAAUGGCAGGACAGCGGCCUGCUGUCCUGCCAUUCUGUAAUGAUGGAAAAUAAAAAAACAGAUAGAAGAUGAGCAACCCUGCGAAAAGUUUAUCAUCUGUUAUCUGUCUCUGUGAUUGUUAUUUUUGAAGGGAAAGGCCUGUCAAGUAUAUUUCUUGUAGGCUUAGCUUGGCAUUCUAAAAAAGUUGGUUAGGCUCUUUUUGAGAAUGCAGAAUUUUUUCCUUUUUAAAAAACAACCAUACUCAUUUUAACAGAAAACCUUAGAAUAAUCUAUUCUUUUGACCCAAAAAAAGUUAAAAAAAGGAAUCAGAAAGAGGCGUAUAGCUAUGGUGCUCCUCUUAUCUGAUUUCCUUAUUCCCUAAAAUUGUAAGCGCUUCAAAAUAUAUGUCAGCGAAAGGAGCUGUCUU ...(((((((((...((.((((((...((((.((((((((((((((((.....))))))))))))))))))))))))))....(((((..((((((((.((((((..(((..........)))..)))))).))))))))...((((.(((((.(((..(((((((((.(((((.((((.......)))))))))........(((((((.((((((..((.....)))))))).)))))))......)))))))))..))).))))).))))....)))))...................))...))))))))).................(((((((((((((((((.((((.......)))).)))))).))))).)))))).................................(.((((.......)))).).. (-135.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGACAGCUCCUUUCGCUGACAUAUAUUUUGAAGCGCUUACAAUUUUAGGGAAUAAGGAAAUCAGAUAAGAGGAGCACCAUAGCUAUACGCCUCUUUCUGAUUCCUUUUUUUAACUUUUUUUGGGUCAAAAGAAUAGAUUAUUCUAAGGUUUUCUGUUAAAAUGAGUAUGGUUGUUUUUUAAAAAGGAAAAAAUUCUGCAUUCUCAAAAAGAGCCUAACCAACUUUUUUAGAAUGCCAAGCUAAGCCUACAAGAAAUAUACUUGACAGGCCUUUCCCUUCAAAAAUAACAAUCACAGAGACAGAUAACAGAUGAUAAACUUUUCGCAGGGUUGCUCAUCUUCUAUCUGUUUUUUUAUUUUCCAUCAUUACAGAAUGGCAGGACAGCAGGCCGCUGUCCUGCCAUUCUAGUAAAUCUGAUAGUUCAAAUCUUCCGACAAA ....((((.......)))).....................(((....((..((.((((((.(((((.(((((((((......))).....)))))))))))))))))...))..))....))).(((...((((((...))))))(((((((.((((((..((((...((((((((((((..((.(((((.......(((((((.((((((...........)))))).)))))))........((((.((((.......))))..)))).))))).))..)))).)))))))).(((((((((((..((((((..(((((......)))))..))))))..)))))))))))..........))))(((((((((((((((((((.....)))))))))))))))).)))....))))))...)))))))..)))... (-126.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 410 81 41 173 130 NC_009009:SSA_2063|5end_Aminopeptidase_PepS,_putative_2064857..2065087_NEGATIVE 400 70 23 231 167 NC_009009:SSA_0397|5end_hypothetical_protein_394657..394887_NEGATIVE 375 70 21 159 112 NC_009009:SSA_0486|5end_Uroporphyrinogen-III_synthase,_putative_486637..486867_POSITIVE 370 72 33 186 142 NC_009009:SSA_2070|5end_Zinc_metalloprotease,_putative_2074574..2074804_NEGATIVE 369 69 25 94 45 NC_009009:SSA_0005|5end_GTP-binding_protein,_putative_3821..4051_POSITIVE 364 206 163 171 124 NC_009009:SSA_0495|5end_ABC-type_oligopeptide/nickel_transport_system,_ATPase_component,_putative_495186..495416_POSITIVE 360 69 34 191 153 NC_009009:SSA_0928|5end_ABC-type_multidrug_transporter,_ATPase_and_permease_components,_putative_936679..936909_POSITIVE 357 74 37 216 169 NC_009009:SSA_1401|5end_Transcriptional_regulator_GntR_family_1413062..1413292_POSITIVE 355 70 28 60 18 NC_009009:SSA_1528|5end_Phosphoglycerate_mutase,_putative_1535135..1535365_POSITIVE 349 79 31 180 132 NC_009009:SSA_0060|5end_Tagatose-6-phosphate_ketose/aldose_isomerase,_putative_61477..61707_POSITIVE 347 79 32 150 109 NC_009009:SSA_2001|5end_Hydroxymethylpyrimidine/phosphomethylpyrimidine_kinase,_putative_1998220..1998450_NEGATIVE 347 69 21 94 38 NC_009009:SSA_0596|5end_hypothetical_protein_586913..587143_POSITIVE 345 65 21 71 17 NC_009009:SSA_1775|5end_Potassium_uptake_protein,_Trk_family,_putative_1761284..1761514_POSITIVE 329 79 38 65 27 NC_009009:SSA_1577|5end_Proline_dipeptidase,_putative_1580294..1580524_POSITIVE 328 65 22 57 16 NC_009009:SSA_1897|5end_Ribosomal_protein_HS6-type_(S12/L30/L7a),_putative_1894450..1894680_NEGATIVE 327 79 31 54 1 NC_009009:SSA_0389|5end_hypothetical_protein_384172..384402_POSITIVE 326 372 334 206 178 NC_009009:SSA_0661|5end_Isoleucyl-tRNA_synthetase,_putative_644876..645106_POSITIVE 324 74 34 71 28 NC_009009:SSA_0834|5end_Accessory_secretory_protein_Asp2,_putative_819510..819740_POSITIVE 322 80 36 178 129 NC_009009:SSA_1099|5end_Calcium_binding_hemolysin-like_protein,_putative_1115361..1115591_POSITIVE 321 64 21 208 171 NC_009009:SSA_2281|5end_hypothetical_protein_2282370..2282600_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 751 79 31 72 21 NC_009009:SSA_0531|3end_Ethanolamine_utilization_protein_eutQ_(cobalamin-dependent_degradation_of_ethanolamine),_putative_527884..528034_POSITIVE 410 81 41 151 108 NC_009009:SSA_2064|3end_hypothetical_protein_2064879..2065029_NEGATIVE 364 79 32 55 11 NC_009009:SSA_2168|3end_Glycerol-3-phosphate_dehydrogenase_[NAD(P)+],_putative_2168563..2168713_POSITIVE 347 69 21 84 28 NC_009009:SSA_0595|3end_hypothetical_protein_586983..587133_POSITIVE 328 65 22 64 23 NC_009009:SSA_1899|3end_hypothetical_protein_1894443..1894593_NEGATIVE 324 74 34 81 38 NC_009009:SSA_0833|3end_Accessory_secretory_protein_Asp1,_putative_819600..819750_POSITIVE 316 72 32 75 13 NC_009009:SSA_0490|3end_Cobalamin_5'-phosphate_synthase,_putative_491044..491194_POSITIVE 314 79 32 60 8 NC_009009:SSA_1201|3end_hypothetical_protein_1226518..1226668_POSITIVE 310 78 37 142 92 NC_009009:SSA_1339|3end_Pneumococcal_histidine_triad_protein_D_precursor,_putative_1351879..1352029_NEGATIVE 310 206 161 82 26 NC_009009:SSA_0030|3end_Phosphoribosylformylglycinamidine_synthase,_putative_36424..36574_POSITIVE 309 70 36 105 73 NC_009009:SSA_0508|3end_Conserved_uncharacterized_protein,_possible_phosphoserine_phosphatase_508064..508214_POSITIVE 305 65 31 76 31 NC_009009:SSA_0097|3end_Multi_antimicrobial_extrusion_(MATE)_family_transporter,_putative_99505..99655_POSITIVE 303 149 101 119 56 NC_009009:SSA_0148|3end_Sugar_ABC_transporter,_ATP-binding_protein,_putative_143467..143617_POSITIVE 301 129 97 108 74 NC_009009:SSA_0294|3end_hypothetical_protein_287772..287922_NEGATIVE 300 67 37 92 59 NC_009009:SSA_1470|3end_3-dehydroquinate_dehydratase,_putative_1476307..1476457_NEGATIVE 295 73 31 74 33 NC_009009:SSA_0899|3end_Conserved_permease,_putative_891161..891311_POSITIVE 295 80 44 80 34 NC_009009:SSA_0110|3end_50S_ribosomal_protein_L2,_putative_110666..110816_POSITIVE 292 74 36 44 3 NC_009009:SSA_1856|3end_hypothetical_protein_1852077..1852227_NEGATIVE 291 79 35 48 8 NC_009009:SSA_0268|3end_Phosphotransferase_system_(PTS)_cellobiose-specific_component_IIB,_putative_262621..262771_POSITIVE 290 69 21 81 40 NC_009009:SSA_0567|3end_Isochorismitase_family_protein,_putative_561442..561592_POSITIVE