Origin IGS: gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agctgatgactctgtaaaaaagctaacctaagaagcccgctctcgttcagctgggcttcttttcccgccaaaagtcattgacaggcttggtggaaactcttataataaagtcgcaagtggcattaagaaaaacttaatactcaagatcaaactaactgctaacagcaatggcctgcagaaaggcgatgctgatgtgatttgatgatgttttttaatagtataaagatgcctgcactgagcaagatctttggtactcgccaatccggcattttagtttccaaactagagacctcactaagcgcttccacatcatagaagaggctagatgggtgaccagacagattgtttggaagccgaattgacatgacaatactaaagagaaaatcttagaagcttgaatgactttaattaaggagataagcaataatgaaaaaagcgtttaaagtggactattttgcaattatgcatccgattgcgtgcatcactctaccgataatggcccaccttttttatcttatggtaaaagccttcttctttgatttgctaaacattctgataagcatttggacaagaaaagaaattaaggaaacaacagatttccttcttcctggattttatctttgtatttttattcttatcatttacttagtgggttttctagtccagttgattatccacattcgcaatcgcaaagaaaactctcttttacctgcttggtgtatgcttacagctttaatcgctcttgttgcaacagttatagacaccttaatccttaaccctgaattgaccccgtatgcttatttcctttcaattgcagcctatattacctatttcttttcaaagagaccaaagattattgaaagctacctgtccaatgctaagatacgttatgctac Mask Tandem Repeat Region ================================================ gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Find is-nt database================================================ Query_seq: SSA_0428:SSA_0429|SSA_0428:SSA_0429:Formimidoylglutamase, putative:Histidine ammonia-lyase, putative:<-<-:428895..429790 896 gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0428:SSA_0429|SSA_0428:SSA_0429:Formimidoylglutamase, putative:Histidine ammonia-lyase, putative:<-<-:428895..429790 896 gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0428:SSA_0429|SSA_0428:SSA_0429:Formimidoylglutamase, putative:Histidine ammonia-lyase, putative:<-<-:428895..429790 896 gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: + Start: 127 End: 252 .............................................................................................................................. M K V S I T V A T R A I K A V S I H Q A G K R E F S L R L R M W I I N W T R K P T K .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 473 End: 682 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M L I S L I K V I Q A S K I F S L V L S C Q F G F Q T I C L V T H L A S S M M W K R L V R S L V W K L K C R I G E Y Q R ...................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 526 End: 735 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. M V M S I R L P N N L S G H P S S L F Y D V E A L S E V S S L E T K M P D W R V P K I L L S A G I F I L L K N I I K S H ................................................................................................................................................................. Protein_Len: 53 Strand: + Start: 636 End: 794 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M A S T K D L A Q C R H L Y T I K K H H Q I T S A S P F C R P L L L A V S L I L S I K F F L M P L A T L L ...................................................................................................... Protein_Len: 41 Strand: - Start: 698 End: 820 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... I V D A D G K Q L G N S N A T L K I K L I L N K K I G S A V K N Y S N G G L R D M ............................................................................ Protein_Len: 38 Strand: - Start: 585 End: 698 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ S T S A S L S T E L K S V L I G S Q R T G F I K S L A P M K I S N F F M M M ...................................................................................................................................................................................................... Protein_Len: 33 Strand: - Start: 538 End: 636 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... N P K W V I Q R T V W R A E E I I H F R K T L D R T Q F S F H R M .................................................................................................................................................................................................................................................................... Protein_Len: 54 Strand: - Start: 124 End: 285 ........................................................................................................................... P N L H R Y S N C C S R N F S Y A Y V L C T F S L K R Q S Q S H P Y D V P S S F G S L L H Y S Y F Y L S L M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 3 End: 473 .. L F D T T E K I E K R T W I S I L I N L L D F F F A K V M L Y F L H A M I P L T I C A I P H M I A F Y D V K F A K K M M ....................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 34 Strand: - Start: 2 End: 103 . L M V Y R L M P C T A K L L R Q D R Q F S I P L I P Q L Q F S I L M ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 90 Start: 791 End: 820 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ATTGACAGGCTTGGTGGAAACTCTTATAAT............................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 51 HP_score: -3.0 Tail_Score: -3.85719 Start: 296 End: 312 Full_Region: AAAATCCAGGAAGAA GGAAAT CTGTT GTTTCC TTAATTTCTTTTCTT .......................................................................................................................................................................................................................................................................................................GGAAATCTGTTGTTTCC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 50 HP_score: -2.3 Tail_Score: -3.76778 Start: 296 End: 312 Full_Region: aagaaaagaaattaa ggaaa caacaga tttcc ttcttcctggatttt .......................................................................................................................................................................................................................................................................................................ggaaacaacagatttcc........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 70 HP_score: -2.7 Tail_Score: -5.1356 Start: 752 End: 764 Full_Region: AGCAGTTAGTTTGAT CTTGA GTA TTAAG TTTTTCTTAATGCCA ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTTGAGTATTAAG.................................................................................................................................... TransTerm Strand: + Conf: 67 HP_score: -7.8 Tail_Score: -3.24122 Start: 838 End: 865 Full_Region: CTTTTGGCGGGAAAA GAAGCCCAGCT GAACGAG AGCGGGCTTC TTAGGTTAGCTTTTT .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GAAGCCCAGCTGAACGAGAGCGGGCTTC............................... Find igs database================================================ Query_seq: SSA_0428:SSA_0429|SSA_0428:SSA_0429:Formimidoylglutamase, putative:Histidine ammonia-lyase, putative:<-<-:428895..429790 896 gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Intra-Species Hit: Count: 1 Min: 1 Max: 896 Len: 896 Subject: NC_009009_SSA_0428_SSA_0429|Formimidoylglutamase, putative:Histidine ammonia-lyase, putative|NEGATIVE:NEGATIVE|[428895,429790]|896 HSP 1 e-value: 0.0 bit: 1776.0 Len: 896 Query Start:1 Query End:896 Subject Strand: POSITIVE Subject Start: 1 Subject End: 896 gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct gtagcataacgtatcttagcattggacaggtagctttcaataatctttggtctctttgaaaagaaataggtaatataggctgcaattgaaaggaaataagcatacggggtcaattcagggttaaggattaaggtgtctataactgttgcaacaagagcgattaaagctgtaagcatacaccaagcaggtaaaagagagttttctttgcgattgcgaatgtggataatcaactggactagaaaacccactaagtaaatgataagaataaaaatacaaagataaaatccaggaagaaggaaatctgttgtttccttaatttcttttcttgtccaaatgcttatcagaatgtttagcaaatcaaagaagaaggcttttaccataagataaaaaaggtgggccattatcggtagagtgatgcacgcaatcggatgcataattgcaaaatagtccactttaaacgcttttttcattattgcttatctccttaattaaagtcattcaagcttctaagattttctctttagtattgtcatgtcaattcggcttccaaacaatctgtctggtcacccatctagcctcttctatgatgtggaagcgcttagtgaggtctctagtttggaaactaaaatgccggattggcgagtaccaaagatcttgctcagtgcaggcatctttatactattaaaaaacatcatcaaatcacatcagcatcgcctttctgcaggccattgctgttagcagttagtttgatcttgagtattaagtttttcttaatgccacttgcgactttattataagagtttccaccaagcctgtcaatgacttttggcgggaaaagaagcccagctgaacgagagcgggcttcttaggttagcttttttacagagtcatcagct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUAGCAUAACGUAUCUUAGCAUUGGACAGGUAGCUUUCAAUAAUCUUUGGUCUCUUUGAAAAGAAAUAGGUAAUAUAGGCUGCAAUUGAAAGGAAAUAAGCAUACGGGGUCAAUUCAGGGUUAAGGAUUAAGGUGUCUAUAACUGUUGCAACAAGAGCGAUUAAAGCUGUAAGCAUACACCAAGCAGGUAAAAGAGAGUUUUCUUUGCGAUUGCGAAUGUGGAUAAUCAACUGGACUAGAAAACCCACUAAGUAAAUGAUAAGAAUAAAAAUACAAAGAUAAAAUCCAGGAAGAAGGAAAUCUGUUGUUUCCUUAAUUUCUUUUCUUGUCCAAAUGCUUAUCAGAAUGUUUAGCAAAUCAAAGAAGAAGGCUUUUACCAUAAGAUAAAAAAGGUGGGCCAUUAUCGGUAGAGUGAUGCACGCAAUCGGAUGCAUAAUUGCAAAAUAGUCCACUUUAAACGCUUUUUUCAUUAUUGCUUAUCUCCUUAAUUAAAGUCAUUCAAGCUUCUAAGAUUUUCUCUUUAGUAUUGUCAUGUCAAUUCGGCUUCCAAACAAUCUGUCUGGUCACCCAUCUAGCCUCUUCUAUGAUGUGGAAGCGCUUAGUGAGGUCUCUAGUUUGGAAACUAAAAUGCCGGAUUGGCGAGUACCAAAGAUCUUGCUCAGUGCAGGCAUCUUUAUACUAUUAAAAAACAUCAUCAAAUCACAUCAGCAUCGCCUUUCUGCAGGCCAUUGCUGUUAGCAGUUAGUUUGAUCUUGAGUAUUAAGUUUUUCUUAAUGCCACUUGCGACUUUAUUAUAAGAGUUUCCACCAAGCCUGUCAAUGACUUUUGGCGGGAAAAGAAGCCCAGCUGAACGAGAGCGGGCUUCUUAGGUUAGCUUUUUUACAGAGUCAUCAGCU ((((.((((.(...(((.((((((..((((............((((((((((((..............(..(((((.((((..(((((..((((.(((((((...(((.....))).(((..((((.((((((((.((((..(((((((....(.(((.......))).)..((((.((((.....((((((((....(((((((((...((((((((((.(((((.((..(((((.(((((.........(((.................))).............(((((.((((((((.....))))))))..)))))))))).)))))..)).)))))...)))))).))))..)))))))))...))))))))....(((((....((....)).)))))....).)))))))..)))).))).((((....))))..))))..)))))))).).)))..))).....))))))).)))))))))..))))............((((.....))))..)))))..)..(((((((((((((((((((........(((..((((((..(((..(((((((...))))))).)))..))).)))..)))))))))))........)))))))))))))).)))))))))))))..)))))))))...).)))).)))).......((..(((((((.((..(((((..((((......))))...)))))......))..)))))))..)).((((((((.....)))))))).......((((((.....(((((((...(((...((((((((......))))))))..(((((((((.(((.......)))))))))))).))).)))))))....))))))....... (-245.43) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCUGAUGACUCUGUAAAAAAGCUAACCUAAGAAGCCCGCUCUCGUUCAGCUGGGCUUCUUUUCCCGCCAAAAGUCAUUGACAGGCUUGGUGGAAACUCUUAUAAUAAAGUCGCAAGUGGCAUUAAGAAAAACUUAAUACUCAAGAUCAAACUAACUGCUAACAGCAAUGGCCUGCAGAAAGGCGAUGCUGAUGUGAUUUGAUGAUGUUUUUUAAUAGUAUAAAGAUGCCUGCACUGAGCAAGAUCUUUGGUACUCGCCAAUCCGGCAUUUUAGUUUCCAAACUAGAGACCUCACUAAGCGCUUCCACAUCAUAGAAGAGGCUAGAUGGGUGACCAGACAGAUUGUUUGGAAGCCGAAUUGACAUGACAAUACUAAAGAGAAAAUCUUAGAAGCUUGAAUGACUUUAAUUAAGGAGAUAAGCAAUAAUGAAAAAAGCGUUUAAAGUGGACUAUUUUGCAAUUAUGCAUCCGAUUGCGUGCAUCACUCUACCGAUAAUGGCCCACCUUUUUUAUCUUAUGGUAAAAGCCUUCUUCUUUGAUUUGCUAAACAUUCUGAUAAGCAUUUGGACAAGAAAAGAAAUUAAGGAAACAACAGAUUUCCUUCUUCCUGGAUUUUAUCUUUGUAUUUUUAUUCUUAUCAUUUACUUAGUGGGUUUUCUAGUCCAGUUGAUUAUCCACAUUCGCAAUCGCAAAGAAAACUCUCUUUUACCUGCUUGGUGUAUGCUUACAGCUUUAAUCGCUCUUGUUGCAACAGUUAUAGACACCUUAAUCCUUAACCCUGAAUUGACCCCGUAUGCUUAUUUCCUUUCAAUUGCAGCCUAUAUUACCUAUUUCUUUUCAAAGAGACCAAAGAUUAUUGAAAGCUACCUGUCCAAUGCUAAGAUACGUUAUGCUAC ((((((((..(((........((......((((((((((((.......))).))))))))).....))....((.((((((((((((..(((.((((..((((....((((((((..(((.((((((.....)))))).).))...................(((((.(.((((......)))).)))))).)))))))).)))).)))).......((((....))))...)))..)))..((((((((((.(((..((((.((((..(((((((....))))))).(((.(((((.((.((((.........))))..))))).)))))..((((((.....))))))..)))).))))..............((((((((......(((((......).))))....(((((((((((((....((..((.((((.((((((((((......((((....)))))))....((((((((((.......(((((.((....))....)))))....((((((((.....(((((((..((((..........(((((.((.((((((.((((((.....((((((.((((.((((.(((........)))....)))))))).))))))......(((((.....))))).)))))).)))))).)).))))).......))))...)))))))......))))))))....)))))))))).....))))))).)))).))....))...((((..((........))..)))).................))))))))))))).......................))))))))....)))))))))))))............)))).))))))).)))..))))).))).. (-225.93) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 883 140 91 231 182 NC_009009:SSA_0428|5end_Formimidoylglutamase,_putative_428804..429034_NEGATIVE 397 870 824 152 99 NC_009009:SSA_2002|5end_tRNA_pseudouridine_synthase_A,_putative_1998959..1999189_NEGATIVE 394 866 822 113 68 NC_009009:SSA_0432|5end_Formate--tetrahydrofolate_ligase,_putative_434991..435221_NEGATIVE 391 750 708 60 18 NC_009009:SSA_0538|5end_PduH_protein,_putative_533794..534024_POSITIVE 376 870 822 54 4 NC_009009:SSA_0452|5end_Nitrogen_utilization_substance_protein_B,_putative_451534..451764_POSITIVE 370 866 822 141 96 NC_009009:SSA_2122|5end_hypothetical_protein_2127607..2127837_NEGATIVE 359 889 841 68 13 NC_009009:SSA_0093|5end_V-type_sodium_ATPase,_subunit_D,_putative_94286..94516_POSITIVE 356 600 555 207 152 NC_009009:SSA_1940|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III,_putative_1927129..1927359_NEGATIVE 355 673 633 162 119 NC_009009:SSA_2340|5end_hypothetical_protein_2342843..2343073_NEGATIVE 355 860 812 60 7 NC_009009:SSA_0210|5end_hypothetical_protein_207757..207987_POSITIVE 353 870 828 74 15 NC_009009:SSA_1111|5end_Branched-chain_amino_acid_transport_system_II_carrier_protein,_putative_1136532..1136762_POSITIVE 345 849 804 172 128 NC_009009:SSA_2184|5end_hypothetical_protein_2183754..2183984_NEGATIVE 344 600 552 57 1 NC_009009:SSA_0405|5end_Transcriptional_regulator,_XRE_family,_putative_401418..401648_POSITIVE 343 848 803 130 83 NC_009009:SSA_1954|5end_Aminotransferase,_class-V,_putative_1951298..1951528_NEGATIVE 342 677 635 137 92 NC_009009:SSA_1678|5end_ABC-type_multidrug_transport_system,_permease_component,_putative_1672181..1672411_NEGATIVE 342 747 711 187 151 NC_009009:SSA_0618|5end_hypothetical_protein_611973..612203_NEGATIVE 341 743 701 65 8 NC_009009:SSA_2230|5end_Ribonucleotide_reductase,_class_III,_anaerobic,_putative_2231158..2231388_NEGATIVE 337 870 828 154 107 NC_009009:SSA_1068|5end_Transcriptional_regulator,_LysR_family_(capsular_polysaccharide_biosynthesis_regulation),_putative_1087355..1087585_POSITIVE 336 860 811 179 130 NC_009009:SSA_0117|5end_50S_ribosomal_protein_L14,_putative_112918..113148_POSITIVE 335 670 629 207 169 NC_009009:SSA_1076|5end_hypothetical_protein_1094227..1094457_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 394 866 822 76 31 NC_009009:SSA_0433|3end_Methenyl_tetrahydrofolate_cyclohydrolase,_putative_435028..435178_NEGATIVE 391 750 708 79 37 NC_009009:SSA_0537|3end_Propanediol_utilization:dioldehydratase_reactivation,_putative_533893..534043_POSITIVE 376 870 822 76 26 NC_009009:SSA_0451|3end_Conserved_uncharacterized_protein_451636..451786_POSITIVE 370 866 822 84 39 NC_009009:SSA_2123|3end_Dimethyladenosine_transferase,_putative_2127664..2127814_NEGATIVE 367 589 541 69 10 NC_009009:SSA_1602|3end_Exoribonuclease,_VacB/Rnb_family,_putative_1607022..1607172_NEGATIVE 361 579 532 149 108 NC_009009:SSA_1124|3end_Oxidoreductase,_putative_1148547..1148697_NEGATIVE 361 890 841 101 42 NC_009009:SSA_0402|3end_Histidine_kinase,_putative_400099..400249_POSITIVE 359 889 841 71 16 NC_009009:SSA_0092|3end_V-type_sodium_ATPase,_subunit_B,_putative_94369..94519_POSITIVE 348 609 561 150 108 NC_009009:SSA_2121|3end_Cell_wall_surface_anchor_family_protein,_putative_2122222..2122372_NEGATIVE 346 758 712 114 56 NC_009009:SSA_1052|3end_hypothetical_protein_1066846..1066996_NEGATIVE 345 875 835 109 60 NC_009009:SSA_1357|3end_hypothetical_protein_1372077..1372227_NEGATIVE 343 848 803 88 41 NC_009009:SSA_1955|3end_ABC-type_Fe-S_cluster_assembly_transporter,_permease_component,_putative_1951340..1951490_NEGATIVE 342 677 635 135 90 NC_009009:SSA_1679|3end_ABC-type_multidrug_transport_system,_ATPase_component,_putative_1672183..1672333_NEGATIVE 342 848 801 133 85 NC_009009:SSA_0212|3end_Phenylalanyl-tRNA_synthetase,_beta_subunit,_putative_209079..209229_POSITIVE 338 867 822 66 15 NC_009009:SSA_1166|3end_hypothetical_protein_1192350..1192500_POSITIVE 337 870 828 64 17 NC_009009:SSA_1067|3end_hypothetical_protein_1087345..1087495_POSITIVE 333 741 702 149 98 NC_009009:SSA_0448|3end_Excinuclease_ATPase_subunit_A,_putative_449391..449541_POSITIVE 328 880 837 61 16 NC_009009:SSA_2101|3end_Amino_acid_ABC_transporter,_periplasmic_amino_acid-binding_protein,_putative_2104509..2104659_NEGATIVE 328 886 845 58 21 NC_009009:SSA_1225|3end_Branched-chain_amino_acid_aminotransferase,_putative_1247589..1247739_NEGATIVE 325 860 811 55 10 NC_009009:SSA_0837|3end_Glucosyltransferase,_putative_825502..825652_POSITIVE