Origin IGS: gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gagttccacaatttgccacttgcttttaaccctcaccaaaaactttagaaatatttgagaaattctttataaatatttgaaagtttggcgctatactagttaacaagaagaggagttttctctcctagtatcatgaaaaggagaattgatc Mask Tandem Repeat Region ================================================ gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Find is-nt database================================================ Query_seq: SSA_0412:SSA_0413|SSA_0412:SSA_0413:ABC-type multidrug transport system (3-component subtilin immunity exporter), ATPase component, putative:Anthranilate/para-aminobenzoate synthases component I/ Chorismate binding enzyme, putative:<-<-:408254..408404 151 gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0412:SSA_0413|SSA_0412:SSA_0413:ABC-type multidrug transport system (3-component subtilin immunity exporter), ATPase component, putative:Anthranilate/para-aminobenzoate synthases component I/ Chorismate binding enzyme, putative:<-<-:408254..408404 151 gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0412:SSA_0413|SSA_0412:SSA_0413:ABC-type multidrug transport system (3-component subtilin immunity exporter), ATPase component, putative:Anthranilate/para-aminobenzoate synthases component I/ Chorismate binding enzyme, putative:<-<-:408254..408404 151 gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Predict ORF larger than 30AA ================================================ gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_0412:SSA_0413|SSA_0412:SSA_0413:ABC-type multidrug transport system (3-component subtilin immunity exporter), ATPase component, putative:Anthranilate/para-aminobenzoate synthases component I/ Chorismate binding enzyme, putative:<-<-:408254..408404 151 gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Intra-Species Hit: Count: 1 Min: 1 Max: 151 Len: 151 Subject: NC_009009_SSA_0412_SSA_0413|ABC-type multidrug transport system (3-component subtilin immunity exporter), ATPase component, putative:Anthranilate/para-aminobenzoate synthases component I/ Chorismate binding enzyme, putative|NEGATIVE:NEGATIVE|[408254,408404]|151 HSP 1 e-value: 1.0E-80 bit: 299.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc gatcaattctccttttcatgatactaggagagaaaactcctcttcttgttaactagtatagcgccaaactttcaaatatttataaagaatttctcaaatatttctaaagtttttggtgagggttaaaagcaagtggcaaattgtggaactc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAUCAAUUCUCCUUUUCAUGAUACUAGGAGAGAAAACUCCUCUUCUUGUUAACUAGUAUAGCGCCAAACUUUCAAAUAUUUAUAAAGAAUUUCUCAAAUAUUUCUAAAGUUUUUGGUGAGGGUUAAAAGCAAGUGGCAAAUUGUGGAACUC .............(((((..((...(((((......)))))((.((((((((((.......(((((((((((.((((((((....((.....)).))))))))..)))))..))))))..)))))...))))).))...))..)))))... (-28.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGUUCCACAAUUUGCCACUUGCUUUUAACCCUCACCAAAAACUUUAGAAAUAUUUGAGAAAUUCUUUAUAAAUAUUUGAAAGUUUGGCGCUAUACUAGUUAACAAGAAGAGGAGUUUUCUCUCCUAGUAUCAUGAAAAGGAGAAUUGAUC ........(((((..((.((((...(((((...(.((((..(((((..(((((((((.(((....))).))))))))).))))))))).)........)))))))))...(((((......))))).............))..)))))... (-27.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 866 70 21 161 112 NC_009009:SSA_0412|5end_ABC-type_multidrug_transport_system_(3-component_subtilin_immunity_exporter),_ATPase_component,_putative_408163..408393_NEGATIVE 309 49 2 144 92 NC_009009:SSA_1244|5end_hypothetical_protein_1268704..1268934_NEGATIVE 288 73 38 231 191 NC_009009:SSA_1276|5end_Conserved_uncharacterized_protein_1298446..1298676_NEGATIVE 275 109 65 181 133 NC_009009:SSA_0407|5end_ABC-type_multidrug_transport_system_(3-component_subtilin_immunity_exporter),_ATPase_component,_putative_403843..404073_NEGATIVE 271 148 101 159 104 NC_009009:SSA_2062|5end_hypothetical_protein_2063521..2063751_NEGATIVE 269 67 26 49 1 NC_009009:SSA_1629|5end_Peptidyl-prolyl_cis-trans_isomerase,_cyclophilin-type,_putative_1630338..1630568_POSITIVE 265 150 108 191 134 NC_009009:SSA_0350|5end_Helicase,_putative_344218..344448_NEGATIVE 265 109 65 177 134 NC_009009:SSA_0253|5end_CAAX_amino_terminal_protease_family_protein,_putative_246289..246519_POSITIVE 262 66 35 185 144 NC_009009:SSA_1483|5end_Sphingosine_kinase_(diacylglycerol_kinase),_putative_1490220..1490450_NEGATIVE 260 50 5 154 99 NC_009009:SSA_0972|5end_hypothetical_protein_979973..980203_POSITIVE 253 74 33 219 180 NC_009009:SSA_0416|5end_5-methyltetrahydropteroyltriglutamate--_homocysteine_methyltransferase,_putative_411890..412120_POSITIVE 248 59 14 67 22 NC_009009:SSA_0238|5end_hypothetical_protein_232481..232711_POSITIVE 246 142 115 76 46 NC_009009:SSA_2196|5end_Conserved_uncharacterized_protein_2197330..2197560_NEGATIVE 246 59 11 100 38 NC_009009:SSA_1901|5end_Conserved_uncharacterized_protein_1896599..1896829_NEGATIVE 240 150 109 201 164 NC_009009:SSA_0656|5end_Cell_division_protein_FtsZ,_putative_640159..640389_POSITIVE 239 88 42 180 129 NC_009009:SSA_2243|5end_Conserved_uncharacterized_protein_2239341..2239571_NEGATIVE 238 109 70 177 138 NC_009009:SSA_0409|5end_ABC-type_multidrug_transport_system,_ATPase_component,_putative_405605..405835_NEGATIVE 235 49 7 136 98 NC_009009:SSA_1712|5end_Arsenate_reductase_(ArsC),_glutaredoxin_family,_putative_1700084..1700314_NEGATIVE 232 146 107 163 127 NC_009009:SSA_1791|5end_Acylphosphatase,_putative_1773676..1773906_POSITIVE 230 62 28 128 92 NC_009009:SSA_0668|5end_hypothetical_protein_650737..650967_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 271 148 101 64 9 NC_009009:SSA_2063|3end_Aminopeptidase_PepS,_putative_2063616..2063766_NEGATIVE 265 150 108 131 74 NC_009009:SSA_0351|3end_Signal_peptidase_I,_putative_344278..344428_NEGATIVE 259 122 84 74 33 NC_009009:SSA_1878|3end_Conserved_uncharacterized_protein_1871943..1872093_NEGATIVE 247 137 91 65 21 NC_009009:SSA_0749|3end_Competence_protein,_putative_731698..731848_POSITIVE 246 142 115 92 62 NC_009009:SSA_2197|3end_hypothetical_protein_2197314..2197464_NEGATIVE 241 67 24 126 76 NC_009009:SSA_1611|3end_Era-like_GTP-binding_protein,_putative_1615133..1615283_NEGATIVE 238 40 6 121 84 NC_009009:SSA_1610|3end_hypothetical_protein_1614004..1614154_NEGATIVE 235 137 93 131 91 NC_009009:SSA_0390|3end_hypothetical_protein_385714..385864_POSITIVE 230 62 28 68 32 NC_009009:SSA_0667|3end_Transcriptional_regulator,_putative_650757..650907_POSITIVE 229 66 37 151 117 NC_009009:SSA_1012|3end_Phosphoenolpyruvate_synthase,_putative_1017314..1017464_POSITIVE 225 69 26 116 62 NC_009009:SSA_1223|3end_30S_ribosomal_protein_S1,_putative_1245667..1245817_NEGATIVE 225 67 21 102 44 NC_009009:SSA_0138|3end_Metal-binding_(Zn)_permease,_putative_134115..134265_POSITIVE 222 65 24 126 70 NC_009009:SSA_2016|3end_Phosphoglycerate_mutase,_putative_2015592..2015742_NEGATIVE 222 57 11 103 55 NC_009009:SSA_1804|3end_NADPH-flavin_oxidoreductase,_putative_1787952..1788102_NEGATIVE 221 55 14 97 66 NC_009009:SSA_2065|3end_hypothetical_protein_2065051..2065201_NEGATIVE 221 63 23 49 8 NC_009009:SSA_1952|3end_ABC-type_Fe-S_cluster_assembly_transporter,_permease_component,_putative_1948212..1948362_NEGATIVE 219 64 24 53 10 NC_009009:SSA_2278|3end_Ukp_protein,_putative_2280410..2280560_NEGATIVE 218 59 11 128 82 NC_009009:SSA_1897|3end_Ribosomal_protein_HS6-type_(S12/L30/L7a),_putative_1894154..1894304_NEGATIVE 217 46 7 60 17 NC_009009:SSA_1836|3end_Superfamily_II_ATP-dependent_DNA/RNA_helicase_required_for_DNA_uptake_(late_competence_protein),_putative_1830938..1831088_NEGATIVE 217 147 104 59 26 NC_009009:SSA_1464|3end_3-phosphoshikimate_1-carboxyvinyltransferase,_putative_1470233..1470383_NEGATIVE