Origin IGS: cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataaaaacctttcaacaataaaaattaaacactccgattaaactgactagtaaatagccaacactacaaacagcctgtgccagcaacatatggtgactagaaatgactgtagtaatgtcaccatcttgtcttatgcacttctaaaatttgttatattgattagaaaccagagcttataaaaactagctaaactcgagagatagacacaattgcgacgaaaaaatgtataaccaattcaacttgttaaggtaagaaagcattatttccaatctattaattattcatctattacctattatgacacaatattccctatagttcaactttttacttgtttttatattacacggactaaaaagttttcaacagttgattgcatttgttttaataagttttaacctttcttggttagataaaaaaagaaccatacagtttagaatctgtatggtttttgccccacttttgtcccatttttaaatcatgacattgaaaatacctaaaatcacttatatttcaatatttttaattgctctcaatatgaaagtcctttccaaatccctgtagggggcatctaaatacaacaggaaaaagccttgatttactaggcttttttgtgtagtcttagcactaaaaaacgatagaaaaaacaccccttctgaactgcaccccaaaagttagacagaaaaaacctaacttttgtggcgcagttcattcgcgtcacatgaagttatttaagcatctcccttttttttattaccattttctgttataataaattgtacttctaaaatagaaaggtcttaag Mask Tandem Repeat Region ================================================ cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Find is-nt database================================================ Query_seq: SSA_0230:SSA_0231|SSA_0230:SSA_0231:hypothetical protein:Conserved uncharacterized protein:<-->:225048..225852 805 cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0230:SSA_0231|SSA_0230:SSA_0231:hypothetical protein:Conserved uncharacterized protein:<-->:225048..225852 805 cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0230:SSA_0231|SSA_0230:SSA_0231:hypothetical protein:Conserved uncharacterized protein:<-->:225048..225852 805 cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 85 End: 201 .................................................................................... M T R M N C A T K V R F F L S N F W G A V Q K G C F F Y R F L V L R L H K K A ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 54 Strand: + Start: 135 End: 296 ...................................................................................................................................... M G C S S E G V F F L S F F S A K T T Q K S L V N Q G F F L L Y L D A P Y R D L E R T F I L R A I K N I E I ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 296 End: 394 ....................................................................................................................................................................................................................................................................................................... M S D F R Y F Q C H D L K M G Q K W G K N H T D S K L Y G S F F I ........................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 32 Strand: + Start: 525 End: 620 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M I N R L E I M L S Y L N K L N W L Y I F S S Q L C L S L E F S ......................................................................................................................................................................................... Protein_Len: 34 Strand: + Start: 571 End: 672 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M G Y T F F R R N C V Y L S S L A S F Y K L W F L I N I T N F R S A ..................................................................................................................................... Protein_Len: 40 Strand: + Start: 672 End: 791 ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M R Q D G D I T T V I S S H H M L L A Q A V C S V G Y L L V S L I G V F N F Y C .............. Protein_Len: 41 Strand: - Start: 630 End: 752 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... L S Q N R I L I V F K L L A Y S L I T V N S C D N R T V M H Q Q C L S N T T N A M ..................................................... Protein_Len: 54 Strand: - Start: 411 End: 572 .......................................................................................................................................................................................................................................................................................................................................................................................................................... F K N F C I C D V T S F K K T R T I Y F C T F L Q V I P F I T D Y Y T I S S Y N I S Q F Y H K R V K V L Q M ......................................................................................................................................................................................................................................... Protein_Len: 45 Strand: - Start: 332 End: 466 ........................................................................................................................................................................................................................................................................................................................................... F H S L L P A F V M C I R F Q I T R K K D L W S L N F S I L V F A I L Q Q F S K L G H L M ................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 44 Strand: - Start: 37 End: 168 .................................... L L F H Y Y F F P L H K F L K M H R S H V A G C F N P K K Q R V K P T C N L L P T K E M ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 482 End: 511 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GTTGAACTATAGGGAATATTGTGTCATAAT...................................................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 33 End: 62 ................................CTTTTTTTTATTACCATTTTCTGTTATAAT....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 56 HP_score: -4.5 Tail_Score: -3.5944 Start: 76 End: 115 Full_Region: tagacagaaaaaacc taacttttgtggcgc agttcattc gcgtcacatgaagtta tttaagcatctccct ...........................................................................taacttttgtggcgcagttcattcgcgtcacatgaagtta.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: - Conf: 78 HP_score: -9.6 Tail_Score: -3.35813 Start: 99 End: 145 Full_Region: aacaccccttctgaa ctgcaccccaaaagttag acagaaaaaac ctaacttttgtggcgcag ttcattcgcgtcaca ..................................................................................................ctgcaccccaaaagttagacagaaaaaacctaacttttgtggcgcag.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 59 HP_score: -6.2 Tail_Score: -3.04144 Start: 187 End: 227 Full_Region: gggggcatctaaata caacaggaaaaagccttg attta ctaggcttttttgtgtag tcttagcactaaaaa ..........................................................................................................................................................................................caacaggaaaaagccttgatttactaggcttttttgtgtag.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 58 HP_score: -4.3 Tail_Score: -3.84185 Start: 190 End: 224 Full_Region: TTAGTGCTAAGACTA CACAAAAAAGCCTAG TAAAT CAAGGCTTTTTCCTG TTGTATTTAGATGCC .............................................................................................................................................................................................CACAAAAAAGCCTAGTAAATCAAGGCTTTTTCCTG..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 57 HP_score: -6.5 Tail_Score: -2.74523 Start: 190 End: 224 Full_Region: ggcatctaaatacaa caggaaaaagccttg attta ctaggcttttttgtg tagtcttagcactaa .............................................................................................................................................................................................caggaaaaagccttgatttactaggcttttttgtg..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 48 HP_score: -4.6 Tail_Score: -2.69459 Start: 193 End: 221 Full_Region: GTGCTAAGACTACAC AAAAAAGCCTAG TAAAT CAAGGCTTTTTC CTGTTGTATTTAGAT ................................................................................................................................................................................................AAAAAAGCCTAGTAAATCAAGGCTTTTTC........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 76 HP_score: -3.6 Tail_Score: -5.40625 Start: 199 End: 215 Full_Region: atacaacaggaaaaa gccttg attta ctaggc ttttttgtgtagtct ......................................................................................................................................................................................................gccttgatttactaggc.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 100 HP_score: -6.5 Tail_Score: -5.71712 Start: 355 End: 384 Full_Region: ACAAAAGTGGGGCAA AAACCATACAGAT TCTA AACTGTATGGTTC TTTTTTTATCTAACC ..................................................................................................................................................................................................................................................................................................................................................................AAACCATACAGATTCTAAACTGTATGGTTC..................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 90 HP_score: -8.2 Tail_Score: -4.92669 Start: 358 End: 381 Full_Region: AAAGTGGGGCAAAAA CCATACAGAT TCTA AACTGTATGG TTCTTTTTTTATCTA .....................................................................................................................................................................................................................................................................................................................................................................CCATACAGATTCTAAACTGTATGG........................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: - Conf: 85 HP_score: -8.2 Tail_Score: -4.58685 Start: 358 End: 381 Full_Region: tagataaaaaaagaa ccatacagtt taga atctgtatgg tttttgccccacttt .....................................................................................................................................................................................................................................................................................................................................................................ccatacagtttagaatctgtatgg........................................................................................................................................................................................................................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 43 HP_score: -3.0 Tail_Score: -2.84229 Start: 746 End: 762 Full_Region: GGCTGTTTGTAGTGT TGGCTA TTTAC TAGTCA GTTTAATCGGAGTGT .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TGGCTATTTACTAGTCA........................................... Find igs database================================================ Query_seq: SSA_0230:SSA_0231|SSA_0230:SSA_0231:hypothetical protein:Conserved uncharacterized protein:<-->:225048..225852 805 cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Intra-Species Hit: Count: 1 Min: 1 Max: 805 Len: 805 Subject: NC_009009_SSA_0230_SSA_0231|hypothetical protein:Conserved uncharacterized protein|NEGATIVE:POSITIVE|[225048,225852]|805 HSP 1 e-value: 0.0 bit: 1507.0 Len: 805 Query Start:1 Query End:805 Subject Strand: POSITIVE Subject Start: 1 Subject End: 805 cttaagacctttctattttagaagtacaatttattataacagaaaatggtaatnnnnnnnngggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttcnnnnnnnatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat cttaagacctttctattttagaagtacaatttattataacagaaaatggtaataaaaaaaagggagatgcttaaataacttcatgtgacgcgaatgaactgcgccacaaaagttaggttttttctgtctaacttttggggtgcagttcagaaggggtgttttttctatcgttttttagtgctaagactacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Inter-species Hit: Count: 2 Min: 188 Max: 805 Len: 618 Subject: smit_SMT_1770_SMT_1771|hypothetical protein:unknown, predicted membrane protein (TMS5)|NEGATIVE:POSITIVE|[1746596,1747576]|981 HSP 1 e-value: 0.0 bit: 1144.0 Len: 614 Query Start:192 Query End:805 Subject Strand: POSITIVE Subject Start: 369 Subject End: 981 ...............................................................................................................................................................................................caaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttcnnnnnnnatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat ...............................................................................................................................................................................................caaaaaagccttgtaaatcaaggctttttcctgttg-atttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaaaaccatacagattctaaactgtatggttctttttttatctaaccaagaaaggttaaaacttattaaaacaaatgcaatcaactgttgaaaactttttagtccgtgtaatataaaaacaagtaaaaagttgaactatagggaatattgtgtcataataggtaatagatgaataattaatagattggaaataatgctttcttaccttaacaagttgaattggttatacattttttcgtcgcaattgtgtctatctctcgagtttagctagtttttataagctctggcttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat HSP 2 e-value: 1.0E-87 bit: 325.0 Len: 168 Query Start:188 Query End:355 Subject Strand: POSITIVE Subject Start: 186 Subject End: 353 ...........................................................................................................................................................................................tacacaaaaaagcctagtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaa.................................................................................................................................................................................................................................................................................................................................................................................................................................................................. ...........................................................................................................................................................................................tacacaaaaaagccttgtaaatcaaggctttttcctgttgtatttagatgccccctacagggatttggaaaggactttcatattgagagcaattaaaaatattgaaatataagtgattttaggtattttcaatgtcatgatttaaaaatgggacaaaagtggggcaaa.................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Subject: smit_SMT_2105_SMT_2106|hypothetical protein predicted by Glimmer/Critica:unknown, predicted membrane protein (TMS5)|POSITIVE:POSITIVE|[1997045,1997302]|258 HSP 1 e-value: 1.0E-81 bit: 305.0 Len: 212 Query Start:594 Query End:805 Subject Strand: POSITIVE Subject Start: 52 Subject End: 258 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................caattgtgtctatctctcgagtttagctagtttttataagctctggtttctaatcaatataacaaattttagaagtgcataagacaagatggtgacattactacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................caattgtgtctatctctcgagtt-agctagtttt-ataagctctggct-ctaatcaatata-caatttagaraagtgcataagacaagatggtgacat-actacagtcatttctagtcaccatatgttgctggcacaggctgtttgtagtgttggctatttactagtcagtttaatcggagtgtttaatttttattgttgaaaggtttttat Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACACAAAAAAGCCUAGUAAAUCAAGGCUUUUUCCUGUUGUAUUUAGAUGCCCCCUACAGGGAUUUGGAAAGGACUUUCAUAUUGAGAGCAAUUAAAAAUAUUGAAAUAUAAGUGAUUUUAGGUAUUUUCAAUGUCAUGAUUUAAAAAUGGGACAAAAGUGGGGCAAAAACCAUACAGAUUCUAAACUGUAUGGUUCUUUUUUUAUCUAACCAAGAAAGGUUAAAACUUAUUAAAACAAAUGCAAUCAACUGUUGAAAACUUUUUAGUCCGUGUAAUAUAAAAACAAGUAAAAAGUUGAACUAUAGGGAAUAUUGUGUCAUAAUAGGUAAUAGAUGAAUAAUUAAUAGAUUGGAAAUAAUGCUUUCUUACCUUAACAAGUUGAAUUGGUUAUACAUUUUUUCGUCGCAAUUGUGUCUAUCUCUCGAGUUUAGCUAGUUUUUAUAAGCUCUGGUUUCUAAUCAAUAUAACAAAUUUUAGAAGUGCAUAAGACAAGAUGGUGACAUUACUACAGUCAUUUCUAGUCACCAUAUGUUGCUGGCACAGGCUGUUUGUAGUGUUGGCUAUUUACUAGUCAGUUUAAUCGGAGUGUUUAAUUUUUAUUGUUGAAAGGUUUUUAU .(((..(((((((((.(.....).)))))))))..)))(((((((...((((((((........(((((((.(.(((((.....))))))..((((((.((((........)))).))))))...)))))))((((.(.(((....))).)))))..)).))))))..((((((((((........))))))))))...........(((((......)))))..............))))))).(((((...((((((.....((.((((..(((.......((.((((((.(((..(((((((((.(...((((((((......((((((.(((...........((((((((((...((((.........(((.(((((((((.(.((.((((..((.......))..)))).)).....).))))))))).))).....))))....)))))))))).............(((((((((.((.......((((....))))......)))))))))))....))).))))))))))))))..).)))))))).)..))).)))))).))....)))..)))).)).....))))))..)))))........... (-138.76) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAAACCUUUCAACAAUAAAAAUUAAACACUCCGAUUAAACUGACUAGUAAAUAGCCAACACUACAAACAGCCUGUGCCAGCAACAUAUGGUGACUAGAAAUGACUGUAGUAAUGUCACCAUCUUGUCUUAUGCACUUCUAAAAUUUGUUAUAUUGAUUAGAAACCAGAGCUUAUAAAAACUAGCUAAACUCGAGAGAUAGACACAAUUGCGACGAAAAAAUGUAUAACCAAUUCAACUUGUUAAGGUAAGAAAGCAUUAUUUCCAAUCUAUUAAUUAUUCAUCUAUUACCUAUUAUGACACAAUAUUCCCUAUAGUUCAACUUUUUACUUGUUUUUAUAUUACACGGACUAAAAAGUUUUCAACAGUUGAUUGCAUUUGUUUUAAUAAGUUUUAACCUUUCUUGGUUAGAUAAAAAAAGAACCAUACAGUUUAGAAUCUGUAUGGUUUUUGCCCCACUUUUGUCCCAUUUUUAAAUCAUGACAUUGAAAAUACCUAAAAUCACUUAUAUUUCAAUAUUUUUAAUUGCUCUCAAUAUGAAAGUCCUUUCCAAAUCCCUGUAGGGGGCAUCUAAAUACAACAGGAAAAAGCCUUGAUUUACUAGGCUUUUUUGUGUA ..........((((..................((..(((((..((.(((.....))).)).....(((((......((((.....(((.((((((((...................))))))))..))).....))))........)))))....)))))..))......((((..........))))......(((((...........((.((((((((((((((........((((((.(((.((((((((((......))))......................))))))...........................((((((((((((((.........))).))..)))))))))...))))))))).)))))))............(((((((......)))))))......(((((((((((((........))))))))))))).......))))))).))...............((((((((((....((((.......))))...))))))))))..)))))....)))).(((((((.((......)).)))))))...........(((.((((((((((.........)))))))))).))). (-107.69) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 343 550 511 231 184 NC_009009:SSA_1540|5end_hypothetical_protein_1545268..1545498_NEGATIVE 338 566 522 215 162 NC_009009:SSA_1557|5end_SRPR,_signal_recognition_particle-docking_protein,_putative_1560040..1560270_NEGATIVE 338 551 511 211 171 NC_009009:SSA_0179|5end_hypothetical_protein_183966..184196_POSITIVE 334 579 534 82 38 NC_009009:SSA_0281|5end_hypothetical_protein_273404..273634_POSITIVE 323 439 398 179 138 NC_009009:SSA_0647|5end_hypothetical_protein_630998..631228_POSITIVE 311 90 43 201 145 NC_009009:SSA_0489|5end_Adenosylcobinamide_kinase_/_adenosylcobinamide_phosphate_guanyltransferase,_putative_489655..489885_POSITIVE 307 561 521 71 29 NC_009009:SSA_1417|5end_SAM-dependent_methyltransferase,_putative_1427993..1428223_NEGATIVE 305 562 526 55 21 NC_009009:SSA_0251|5end_D-tyrosyl-tRNA(Tyr)_deacylase,_putative_244455..244685_POSITIVE 305 562 522 62 16 NC_009009:SSA_0236|5end_Chromosome_segregation_helicase,_putative_232443..232673_NEGATIVE 304 558 519 200 166 NC_009009:SSA_1953|5end_NifU_family_protein,_putative_1950079..1950309_NEGATIVE 297 562 521 187 141 NC_009009:SSA_1404|5end_hypothetical_protein_1418723..1418953_NEGATIVE 294 63 28 40 1 NC_009009:SSA_0188|5end_hypothetical_protein_190798..191028_POSITIVE 293 43 13 189 163 NC_009009:SSA_2098|5end_ABC-type_arginine/histidine_transporter,_permease_protein,_putative_2103617..2103847_NEGATIVE 293 550 503 225 188 NC_009009:SSA_0931|5end_hypothetical_protein_940281..940511_POSITIVE 293 540 495 231 186 NC_009009:SSA_0202|5end_hypothetical_protein_200534..200764_POSITIVE 291 576 532 229 187 NC_009009:SSA_1569|5end_ABC_transporter_membrane-spanning_permease,_arginine/histidine_transport,_putative_1571891..1572121_POSITIVE 291 562 524 100 67 NC_009009:SSA_0636|5end_N-(5'-phosphoribosyl)anthranilate_isomerase,_putative_625184..625414_POSITIVE 288 457 412 86 44 NC_009009:SSA_2216|5end_LPS_biosynthesis_protein,_putative_2218080..2218310_NEGATIVE 287 578 534 47 1 NC_009009:SSA_1129|5end_Periplasmic_iron_transport_lipoprotein,_putative_1154925..1155155_POSITIVE 287 564 521 208 157 NC_009009:SSA_0811|5end_hypothetical_protein_792469..792699_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 550 503 127 90 NC_009009:SSA_0929|3end_ABC-type_multidrug_transporter,_ATPase_and_permease_components,_putative_940263..940413_POSITIVE 291 562 524 71 38 NC_009009:SSA_0635|3end_Indole-3-glycerol_phosphate_synthase,_putative_625235..625385_POSITIVE 288 457 412 80 38 NC_009009:SSA_2217|3end_Cps9H,_putative_2218086..2218236_NEGATIVE 281 64 25 80 41 NC_009009:SSA_2049|3end_Polyribonucleotide_nucleotidyltransferase,_putative_2050693..2050843_NEGATIVE 280 550 502 68 23 NC_009009:SSA_0486|3end_Uroporphyrinogen-III_synthase,_putative_487442..487592_POSITIVE 278 547 505 52 9 NC_009009:SSA_2277|3end_DNA_segregation_ATPase_FtsK/SpoIIIE_family_protein,_putative_2275983..2276133_NEGATIVE 275 560 515 150 101 NC_009009:SSA_1405|3end_Copper_homeostasis_protein_CutC,_putative_1418758..1418908_NEGATIVE 274 58 11 147 97 NC_009009:SSA_0593|3end_Rhodanese-like_sulfurtransferase,_putative_585551..585701_POSITIVE 274 561 521 52 23 NC_009009:SSA_0109|3end_50S_ribosomal_protein_L23,_putative_109815..109965_POSITIVE 272 588 541 72 33 NC_009009:SSA_0197|3end_Dihydropteroate_synthase,_putative_196979..197129_POSITIVE 271 576 535 59 19 NC_009009:SSA_0334|3end_Diphosphomevalonate_decarboxylase,_putative_328882..329032_POSITIVE 270 548 501 125 71 NC_009009:SSA_1991|3end_Pneumococcal_histidine_triad_protein_A,_putative_1986340..1986490_NEGATIVE 268 190 152 78 42 NC_009009:SSA_2271|3end_hypothetical_protein_2272238..2272388_NEGATIVE 268 579 535 101 61 NC_009009:SSA_0141|3end_Copper_chaperone,_putative_137191..137341_POSITIVE 263 560 513 136 84 NC_009009:SSA_2019|3end_hypothetical_protein_2018812..2018962_NEGATIVE 263 553 512 128 85 NC_009009:SSA_1860|3end_Penicillin-binding_protein_1A,_putative_1856520..1856670_POSITIVE 263 70 25 123 78 NC_009009:SSA_1537|3end_Conserved_uncharacterized_protein_1544229..1544379_NEGATIVE 263 557 512 65 24 NC_009009:SSA_1121|3end_Cytochrome_C-type_biogenesis_protein,_putative_1147936..1148086_POSITIVE 261 564 526 149 100 NC_009009:SSA_0212|3end_Phenylalanyl-tRNA_synthetase,_beta_subunit,_putative_209079..209229_POSITIVE 259 550 522 28 2 NC_009009:SSA_1606|3end_Dephospho-CoA_kinase,_putative_1611829..1611979_NEGATIVE