Origin IGS: aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctttttctcctaaactattttactctctatttagctgatcagtaaatcagctaaattgaacacgagctatggtctgtgtgaaaaagacaaatcttcctagagtgttaacactctttgtcagatttcctattttcacattgcccatgacgctctttgtatcttgatt Mask Tandem Repeat Region ================================================ aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Find is-nt database================================================ Query_seq: SSA_0120:SSA_0121|SSA_0120:SSA_0121:30S ribosomal protein S8, putative:hypothetical protein:->->:115157..115322 166 aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0120:SSA_0121|SSA_0120:SSA_0121:30S ribosomal protein S8, putative:hypothetical protein:->->:115157..115322 166 aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0120:SSA_0121|SSA_0120:SSA_0121:30S ribosomal protein S8, putative:hypothetical protein:->->:115157..115322 166 aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 22 End: 117 ..................... M G N V K I G N L T K S V N T L G R F V F F T Q T I A R V Q F S ................................................. Protein_Len: 32 Strand: - Start: 6 End: 98 ..... * S V F L T M P L T F I P F R V F L T L V R P L N T K K V C V M .................................................................... aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_0120:SSA_0121|SSA_0120:SSA_0121:30S ribosomal protein S8, putative:hypothetical protein:->->:115157..115322 166 aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Intra-Species Hit: Count: 1 Min: 1 Max: 166 Len: 166 Subject: NC_009009_SSA_0120_SSA_0121|30S ribosomal protein S8, putative:hypothetical protein|POSITIVE:POSITIVE|[115157,115322]|166 HSP 1 e-value: 2.0E-89 bit: 329.0 Len: 166 Query Start:1 Query End:166 Subject Strand: POSITIVE Subject Start: 1 Subject End: 166 aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag aatcaagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaatttagctgatttactgatcagctaaatagagagtaaaatagtttaggagaaaaag Inter-species Hit: Count: 11 Min: 5 Max: 114 Len: 110 Subject: NC_008024_MGAS10750_Spy0062_MGAS10750_Spy0063|SSU ribosomal protein S8P:LSU ribosomal protein L6P|POSITIVE:POSITIVE|[72982,73183]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_008023_MGAS2096_Spy0061_MGAS2096_Spy0062|SSU ribosomal protein S8P:LSU ribosomal protein L6P|POSITIVE:POSITIVE|[71486,71687]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_008021_MGAS9429_Spy0058_MGAS9429_Spy0059|SSU ribosomal protein S8P:LSU ribosomal protein L6P|POSITIVE:POSITIVE|[71510,71711]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_007297_M5005_Spy_0058_M5005_Spy_0059|30S ribosomal protein S8:50S ribosomal protein L6|POSITIVE:POSITIVE|[71701,71902]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_007296_M28_Spy0057_M28_Spy0058|30S ribosomal protein S8:50S ribosomal protein L6|POSITIVE:POSITIVE|[71188,71389]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_006086_M6_Spy0107_M6_Spy0108|30S ribosomal protein S8:50S ribosomal protein L6|POSITIVE:POSITIVE|[112563,112763]|201 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_004606_SPs0056_SPs0057|30S ribosomal protein S8:50S ribosomal protein L6|POSITIVE:POSITIVE|[65901,66102]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_004070_SpyM3_0054_SpyM3_0055|30S ribosomal protein S8:50S ribosomal protein L6|POSITIVE:POSITIVE|[71902,72103]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_002737_SPy0065_SPy0066|30S ribosomal protein S8:50S ribosomal protein L6|POSITIVE:POSITIVE|[71144,71345]|202 HSP 1 e-value: 8.0E-30 bit: 131.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_008022_MGAS10270_Spy0060_MGAS10270_Spy0061|SSU ribosomal protein S8P:LSU ribosomal protein L6P|POSITIVE:POSITIVE|[71881,72082]|202 HSP 1 e-value: 2.0E-27 bit: 123.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttatactcaaggaagatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Subject: NC_003485_spyM18_0066_spyM18_0067|30S ribosomal protein S8:50S ribosomal protein L6|POSITIVE:POSITIVE|[71793,71994]|202 HSP 1 e-value: 2.0E-27 bit: 123.0 Len: 110 Query Start:5 Query End:114 Subject Strand: POSITIVE Subject Start: 4 Subject End: 113 ....aagatacaaagagcgtcatgggcaatgtgaaaataggaaatctgacaaagagtgttaacactctaggaagatttgtctttttcacacagaccatagctcgtgttcaattt.................................................... ....aagatactaagagcgtcgtggacaaagtgaaaataggaaatctgacgtagcgtgtttacactcaaggaggatttatctttttcacacagaccacagctcgtgttcaattt.................................................... Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGAUACAAAGAGCGUCAUGGGCAAUGUGAAAAUAGGAAAUCUGACAAAGAGUGUUAACACUCUAGGAAGAUUUGUCUUUUUCACACAGACCAUAGCUCGUGUUCAAUUU ..((.(((..((((...((((....((((((((..(.((((((..(..((((((....)))))).)..)))))).)..))))))))....)))).)))).)))))..... (-36.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUUGAACACGAGCUAUGGUCUGUGUGAAAAAGACAAAUCUUCCUAGAGUGUUAACACUCUUUGUCAGAUUUCCUAUUUUCACAUUGCCCAUGACGCUCUUUGUAUCUU .....(((((.(((((((((.(.(((((((((....((((((..(.((((((....))))))..)..))))))....))))))))).).)))))..))))..))).)).. (-33.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 394 109 61 201 152 NC_009009:SSA_1226|5end_ParC,_putative_1251233..1251463_NEGATIVE 360 109 61 219 170 NC_009009:SSA_1313|5end_hypothetical_protein_1330548..1330778_NEGATIVE 282 107 63 68 17 NC_009009:SSA_1092|5end_Dihydrofolate_reductase,_putative_1109599..1109829_POSITIVE 269 90 42 67 18 NC_009009:SSA_0041|5end_hypothetical_protein_47662..47892_POSITIVE 264 80 31 172 124 NC_009009:SSA_2005|5end_Chaperone_protein_dnaJ,_putative_2006384..2006614_NEGATIVE 261 100 51 144 92 NC_009009:SSA_0365|5end_Small-conductance_mechanosensitive_efflux_channel,_putative_356621..356851_POSITIVE 243 106 62 197 152 NC_009009:SSA_0241|5end_Ribosomal_protein_L11_methyltransferase,_putative_234021..234251_POSITIVE 242 97 59 54 4 NC_009009:SSA_2277|5end_DNA_segregation_ATPase_FtsK/SpoIIIE_family_protein,_putative_2280407..2280637_NEGATIVE 238 47 2 90 42 NC_009009:SSA_1586|5end_hypothetical_protein_1588182..1588412_NEGATIVE 236 102 63 179 134 NC_009009:SSA_1203|5end_hypothetical_protein_1226985..1227215_POSITIVE 235 47 9 216 165 NC_009009:SSA_0366|5end_hypothetical_protein_357506..357736_POSITIVE 233 101 66 39 5 NC_009009:SSA_1602|5end_Exoribonuclease,_VacB/Rnb_family,_putative_1609370..1609600_NEGATIVE 230 103 63 160 117 NC_009009:SSA_0188|5end_hypothetical_protein_190798..191028_POSITIVE 226 99 59 143 92 NC_009009:SSA_1798|5end_hypothetical_protein_1780513..1780743_NEGATIVE 226 38 1 133 96 NC_009009:SSA_0914|5end_Phenylalanyl-tRNA_synthetase_beta_chain,_putative_924952..925182_POSITIVE 223 54 11 80 48 NC_009009:SSA_0411|5end_hypothetical_protein_407238..407468_NEGATIVE 222 49 3 70 14 NC_009009:SSA_0875|5end_Rod_shape_determining_protein_(cell-cycle_protein),_putative_868928..869158_POSITIVE 222 41 1 190 140 NC_009009:SSA_0784|5end_Proton-translocating_ATPase,_F0_sector,_subunit_b,_putative_767550..767780_POSITIVE 221 97 51 191 143 NC_009009:SSA_0279|5end_Sugar-binding_transcriptional_repressor_(DeoR),_putative_272319..272549_POSITIVE 220 38 1 58 21 NC_009009:SSA_1258|5end_Purine_nucleoside_phosphorylase,_family_1,_putative_1284652..1284882_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 332 99 51 76 25 NC_009009:SSA_0120|3end_30S_ribosomal_protein_S8,_putative_115096..115246_POSITIVE 243 106 62 56 11 NC_009009:SSA_0240|3end_Acetyltransferase,_GNAT_family,_putative_233960..234110_POSITIVE 242 97 59 51 1 NC_009009:SSA_2278|3end_Ukp_protein,_putative_2280410..2280560_NEGATIVE 230 103 63 134 91 NC_009009:SSA_0187|3end_Competence_protein_ComYD,_putative_190852..191002_POSITIVE 228 47 5 74 38 NC_009009:SSA_0170|3end_hypothetical_protein_168130..168280_NEGATIVE 226 99 59 143 92 NC_009009:SSA_1799|3end_Acetyltransferase,_putative_1780513..1780663_NEGATIVE 223 54 11 63 31 NC_009009:SSA_0412|3end_ABC-type_multidrug_transport_system_(3-component_subtilin_immunity_exporter),_ATPase_component,_putative_407255..407405_NEGATIVE 222 49 3 57 1 NC_009009:SSA_0874|3end_hypothetical_protein_868995..869145_POSITIVE 213 66 23 70 34 NC_009009:SSA_0507|3end_hypothetical_protein_506442..506592_POSITIVE 211 106 64 139 102 NC_009009:SSA_1102|3end_hypothetical_protein_1123807..1123957_POSITIVE 209 103 63 151 114 NC_009009:SSA_1276|3end_Conserved_uncharacterized_protein_1298156..1298306_NEGATIVE 207 109 61 116 71 NC_009009:SSA_1408|3end_hypothetical_protein_1420406..1420556_NEGATIVE 206 100 64 60 30 NC_009009:SSA_0467|3end_Cobalt-precorrin-6A_synthase_[deacetylating],_putative_471795..471945_POSITIVE 202 102 62 109 74 NC_009009:SSA_1368|3end_hypothetical_protein_1383748..1383898_NEGATIVE 200 105 61 131 89 NC_009009:SSA_1346|3end_hypothetical_protein_1364151..1364301_NEGATIVE 200 103 61 80 19 NC_009009:SSA_0672|3end_hypothetical_protein_656044..656194_POSITIVE 199 56 12 84 39 NC_009009:SSA_0179|3end_hypothetical_protein_184834..184984_POSITIVE 198 54 12 48 1 NC_009009:SSA_1589|3end_ABC-type_antimicrobial_peptide_transport_system,_ATPase_component,_putative_1591907..1592057_NEGATIVE 198 55 12 45 5 NC_009009:SSA_1049|3end_ABC_transporter_membrane-spanning_permease-spermidine/putrescine_transport,_putative_1064971..1065121_POSITIVE 197 58 12 73 35 NC_009009:SSA_0445|3end_hypothetical_protein_444776..444926_POSITIVE