Origin IGS: aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ctttttctccttttcgtctatttaagataataggctagctccacaagaaaaccgacaggtgattgcgtggcaatgcaaccgagcgtgtcgcaacctcttgcatcaaagctacacgctgtatttttacagcaccatagtatcttaacacaaagaaccagtcattgcaagggatttgagtaattttttaaagatttttaggataattttttatgaaagtgttttctaaacaatttataagtcttgtagatcattatagagaagaaaatttagacttttaacctatctattcgagtttgtattcagaaaatacaaaatctcactgacgcacatcagtgagattagaaacgaaaatttacccttggctactttgtt Mask Tandem Repeat Region ================================================ aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Find is-nt database================================================ Query_seq: SSA_0105:SSA_0106|SSA_0105:SSA_0106:Uridine kinase, putative:30S ribosomal protein S10, putative:->->:107540..107911 372 aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0105:SSA_0106|SSA_0105:SSA_0106:Uridine kinase, putative:30S ribosomal protein S10, putative:->->:107540..107911 372 aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0105:SSA_0106|SSA_0105:SSA_0106:Uridine kinase, putative:30S ribosomal protein S10, putative:->->:107540..107911 372 aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: + Start: 192 End: 335 ............................................................................................................................................................................................... M T Q I P C N D W F F V L R Y Y G A V K I Q R V A L M Q E V A T R S V A L P R N H L S V F L W S ..................................... Protein_Len: 46 Strand: + Start: 215 End: 352 ...................................................................................................................................................................................................................... M V L C V K I L W C C K N T A C S F D A R G C D T L G C I A T Q S P V G F L V E L A Y Y L K .................... Protein_Len: 37 Strand: - Start: 196 End: 306 ................................................................................................................................................................................................... E F G K C H S T R Q T L I S H H Q L F V A H L K S A L P Q S V S P Q M A M .................................................................. aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 94 End: 123 .............................................................................................GTTAAAAGTCTAAATTTTCTTCTCTATAAT......................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 205 End: 234 ............................................................................................................................................................................................................CTTGCAATGACTGGTTCTTTGTGTTAAGAT.......................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 82 Start: 172 End: 201 ...........................................................................................................................................................................TTTGAGTAATTTTTTAAAGATTTTTAGGAT........................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_0105:SSA_0106|SSA_0105:SSA_0106:Uridine kinase, putative:30S ribosomal protein S10, putative:->->:107540..107911 372 aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcataaaaaattatcctaaaaatctttaaaaaattactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Intra-Species Hit: Count: 1 Min: 1 Max: 372 Len: 372 Subject: NC_009009_SSA_0105_SSA_0106|Uridine kinase, putative:30S ribosomal protein S10, putative|POSITIVE:POSITIVE|[107540,107911]|372 HSP 1 e-value: 2.0E-97 bit: 357.0 Len: 180 Query Start:193 Query End:372 Subject Strand: POSITIVE Subject Start: 193 Subject End: 372 ................................................................................................................................................................................................ttactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag ................................................................................................................................................................................................ttactcaaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag HSP 2 e-value: 2.0E-87 bit: 323.0 Len: 163 Query Start:1 Query End:163 Subject Strand: POSITIVE Subject Start: 1 Subject End: 163 aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcat................................................................................................................................................................................................................. aacaaagtagccaagggtaaattttcgtttctaatctcactgatgtgcgtcagtgagattttgtattttctgaatacaaactcgaatagataggttaaaagtctaaattttcttctctataatgatctacaagacttataaattgtttagaaaacactttcat................................................................................................................................................................................................................. Inter-species Hit: Count: 19 Min: 198 Max: 372 Len: 175 Subject: smit_SMT_168_SMT_169|anaerobic ribonucleoside-triphosphate reductase activating protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[114401,114654]|254 HSP 1 e-value: 2.0E-47 bit: 190.0 Len: 175 Query Start:198 Query End:372 Subject Strand: POSITIVE Subject Start: 82 Subject End: 254 .....................................................................................................................................................................................................caaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacct-gtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag .....................................................................................................................................................................................................caaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaat-tacagcct-tagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaa-caccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_008533_SPD_0191_SPD_0192|hypothetical protein:ribosomal protein S10|POSITIVE:POSITIVE|[195285,195550]|266 HSP 1 e-value: 6.0E-45 bit: 182.0 Len: 175 Query Start:198 Query End:372 Subject Strand: POSITIVE Subject Start: 94 Subject End: 266 .....................................................................................................................................................................................................caaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacct-gtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag .....................................................................................................................................................................................................caaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaat-tacagcct-tagttttgatgcaagaggttgcgacacgctcggttgcattgccacgcaa-caccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_003028_SP_0207_SP_0208|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[192776,193041]|266 HSP 1 e-value: 6.0E-45 bit: 182.0 Len: 175 Query Start:198 Query End:372 Subject Strand: POSITIVE Subject Start: 94 Subject End: 266 .....................................................................................................................................................................................................caaatcccttgcaatgactggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacct-gtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag .....................................................................................................................................................................................................caaatcccttgcaatcgcaggggctttgtgttattctattatggtgctgtaaat-tacagcct-tagttttgatgcaagaggttgcgacacgctcggttgcattgccacgcaa-caccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_003098_spr0186_spr0187|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[195285,195550]|266 HSP 1 e-value: 1.0E-42 bit: 174.0 Len: 107 Query Start:266 Query End:372 Subject Strand: POSITIVE Subject Start: 160 Subject End: 266 .........................................................................................................................................................................................................................................................................tttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacct-gtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag .........................................................................................................................................................................................................................................................................tttgatgcaagaggttgcgacacgctcggttgcattgccacgcaa-caccgcgtcggttttcttgtggagctagcctattatcttaaatagacgaaaaggagaaaaag Subject: NC_004350_SMU.2026c_SMU.2027|30S ribosomal protein S10:putative transcriptional regulator|NEGATIVE:POSITIVE|[1893131,1893647]|517 HSP 1 e-value: 2.0E-38 bit: 161.0 Len: 121 Query Start:239 Query End:359 Subject Strand: NEGATIVE Subject Start: 210 Subject End: 329 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaat-cagcctttagctttgatacaagaggttgcgatacgctcggttgcattgccacgcaaaagcctgtcggttttcttgtggagctagcctattattttaaatagacga............. Subject: NC_006086_M6_Spy0091_M6_Spy0092|Na+ driven multidrug efflux pump:30S ribosomal protein S10|POSITIVE:POSITIVE|[104934,105131]|198 HSP 1 e-value: 8.0E-35 bit: 149.0 Len: 123 Query Start:237 Query End:359 Subject Strand: POSITIVE Subject Start: 67 Subject End: 187 ............................................................................................................................................................................................................................................tatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ............................................................................................................................................................................................................................................tatggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008024_MGAS10750_Spy0043_MGAS10750_Spy0044|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[65353,65551]|199 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 70 Subject End: 188 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008023_MGAS2096_Spy0045_MGAS2096_Spy0046|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[63854,64051]|198 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 69 Subject End: 187 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008022_MGAS10270_Spy0044_MGAS10270_Spy0045|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[64251,64448]|198 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 69 Subject End: 187 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_008021_MGAS9429_Spy0042_MGAS9429_Spy0043|Na+ driven multidrug efflux pump:SSU ribosomal protein S10P|POSITIVE:POSITIVE|[63879,64076]|198 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 69 Subject End: 187 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_007297_M5005_Spy_0042_M5005_Spy_0043|Na+ driven multidrug efflux pump:30S ribosomal protein S10|POSITIVE:POSITIVE|[64101,64300]|200 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 71 Subject End: 189 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_007296_M28_Spy0041_M28_Spy0042|Na+ driven multidrug efflux pump:30S ribosomal protein S10|POSITIVE:POSITIVE|[63557,63754]|198 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 69 Subject End: 187 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_004606_SPs0039_SPs0040|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[58271,58468]|198 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 69 Subject End: 187 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_004070_SpyM3_0038_SpyM3_0039|putative multi antimicrobial extrusion (MATE) family transporter:30S ribosomal protein S10|POSITIVE:POSITIVE|[64272,64469]|198 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 69 Subject End: 187 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_003485_spyM18_0048_spyM18_0049|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[64163,64360]|198 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 69 Subject End: 187 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_002737_SPy0045_SPy0047|hypothetical protein:30S ribosomal protein S10|POSITIVE:POSITIVE|[63512,63711]|200 HSP 1 e-value: 1.0E-33 bit: 145.0 Len: 121 Query Start:239 Query End:359 Subject Strand: POSITIVE Subject Start: 71 Subject End: 189 ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacga............. ..............................................................................................................................................................................................................................................tggtgctgtaaaaatacagctt-tagcaatgatacaagaggttgcgacacgctcggttgcattgccacgcaa-caggtgtcggttttcttgaggagctagcctattatcgtaaatagacga............. Subject: NC_006449_str1935_str1936|30S ribosomal protein S10:Holliday junction DNA helicase RuvB|NEGATIVE:NEGATIVE|[1711847,1712089]|243 HSP 1 e-value: 3.0E-28 bit: 127.0 Len: 173 Query Start:199 Query End:371 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 171 ......................................................................................................................................................................................................aaatcccttgcaatgact-ggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaa-aggagaaaaa. ......................................................................................................................................................................................................aaatcccttgcaatgaataggttcttc-tgttattatattatggtgctgttaaaa--cagtgcttagctatgatacaagaggttgcgacacgcctggttgcactgccacgcaatc-cgtgttggttttcttgaggagctagcctattatcttaaatagacgaaagaggagaaaaa. Subject: NC_008532_STER_1908_STER_1909|Ribosomal protein S10:Holliday junction resolvasome, helicase subunit|NEGATIVE:NEGATIVE|[1769490,1769732]|243 HSP 1 e-value: 1.0E-27 bit: 125.0 Len: 110 Query Start:262 Query End:371 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 110 .....................................................................................................................................................................................................................................................................tagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaa-aggagaaaaa. .....................................................................................................................................................................................................................................................................tagctatgatacaagaggttgcgacacgcctggttgcactgccacgcaatc-cgtgttggttttcttgaggagctagcctattatcttaaatagacgaaagaggagaaaaa. Subject: NC_006448_stu1935_stu1936|30S ribosomal protein S10:Holliday junction DNA helicase RuvB|NEGATIVE:NEGATIVE|[1711223,1711465]|243 HSP 1 e-value: 7.0E-26 bit: 119.0 Len: 173 Query Start:199 Query End:371 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 171 ......................................................................................................................................................................................................aaatcccttgcaatgact-ggttctttgtgttaagatactatggtgctgtaaaaatacagcgtgtagctttgatgcaagaggttgcgacacgctcggttgcattgccacgcaatcacctgtcggttttcttgtggagctagcctattatcttaaatagacgaaa-aggagaaaaa. ......................................................................................................................................................................................................aaatcccttgcaatgaataggttcttc-tgttatcatattatggtgctgttaaaa--cagtgcttagctatgatacaagaggttgcgacacgcctggttgcactgccacgcaatc-cgtgttggttttcttgaggagttagcctattatcttaaatagacgaaagaggagaaaaa. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAAAUCCCUUGCAAUGACUGGUUCUUUGUGUUAAGAUACUAUGGUGCUGUAAAAAUACAGCGUGUAGCUUUGAUGCAAGAGGUUGCGACACGCUCGGUUGCAUUGCCACGCAAUCACCUGUCGGUUUUCUUGUGGAGCUAGCCUAUUAUCUUAAAUAGACGAAAAGGAGAAAAAG .................((..((((((((.((((((((.((.(((((((((....))))))...(((((((...((((((((...(((((.(...((((((........)))))).).)))))..)))))))))))))))))))).))))))))))))).)))..))........ (-47.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUUUUUCUCCUUUUCGUCUAUUUAAGAUAAUAGGCUAGCUCCACAAGAAAACCGACAGGUGAUUGCGUGGCAAUGCAACCGAGCGUGUCGCAACCUCUUGCAUCAAAGCUACACGCUGUAUUUUUACAGCACCAUAGUAUCUUAACACAAAGAACCAGUCAUUGCAAGGGAUUUG ..((((((.......(((((((......)))))))..........)))))).(((((.((..((((((....))))))....)).)))))....((((((((.....(((....((((((....)))))).........((((......))))...)))...))))))))..... (-43.43) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 361 126 81 225 181 NC_009009:SSA_1401|5end_Transcriptional_regulator_GntR_family_1413062..1413292_POSITIVE 352 97 58 217 166 NC_009009:SSA_1890|5end_Acetyltransferase,_putative_1888145..1888375_NEGATIVE 346 170 122 170 124 NC_009009:SSA_1370|5end_CAAX_amino_terminal_protease_family,_putative_1386968..1387198_NEGATIVE 346 109 61 230 191 NC_009009:SSA_0055|5end_PTS_system,_IIC_component,_putative_59034..59264_POSITIVE 344 140 92 219 169 NC_009009:SSA_1546|5end_Prolipoprotein_diacylglyceryl_transferase,_putative_1550052..1550282_NEGATIVE 340 98 57 161 121 NC_009009:SSA_0678|5end_Transcriptional_regulator,_ArgR_family,_putative_660274..660504_POSITIVE 330 138 91 100 46 NC_009009:SSA_1066|5end_ABC-type_oligopeptide_transport_system,_putative_1084335..1084565_POSITIVE 330 143 102 226 186 NC_009009:SSA_0356|5end_Dipeptidase,_putative_349259..349489_POSITIVE 326 124 81 49 8 NC_009009:SSA_0335|5end_Phosphomevalonate_kinase,_putative_328792..329022_POSITIVE 325 137 91 165 112 NC_009009:SSA_0454|5end_Lactose_operon_transcriptional_repressor,_LacI_family,_putative_457015..457245_NEGATIVE 321 124 82 217 170 NC_009009:SSA_0811|5end_hypothetical_protein_792469..792699_POSITIVE 320 144 103 105 69 NC_009009:SSA_0977|5end_3-isopropylmalate_dehydrogenase,_putative_982771..983001_POSITIVE 318 106 61 229 175 NC_009009:SSA_1153|5end_HemK_protein,_putative_1181838..1182068_POSITIVE 316 120 71 78 18 NC_009009:SSA_1266|5end_crcB-like_protein_1,_putative_1291000..1291230_NEGATIVE 315 129 81 214 149 NC_009009:SSA_0981|5end_3-isopropylmalate_dehydratase_small_subunit,_putative_985396..985626_POSITIVE 314 129 81 71 7 NC_009009:SSA_1607|5end_Formamidopyrimidine-DNA_glycosylase,_putative_1613255..1613485_NEGATIVE 314 109 61 164 117 NC_009009:SSA_1485|5end_Citrulline_cluster-linked_gene,_putative_1492809..1493039_NEGATIVE 312 141 101 70 20 NC_009009:SSA_1614|5end_Acetyltransferase,_GNAT_family,_putative_1618045..1618275_NEGATIVE 312 118 80 34 1 NC_009009:SSA_0467|5end_Cobalt-precorrin-6A_synthase_[deacetylating],_putative_470588..470818_POSITIVE 309 110 67 58 13 NC_009009:SSA_1931|5end_Acetyl-coenzyme_A_carboxylase_carboxyl_transferase_subunit_beta,_putative_1919407..1919637_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 347 136 91 143 92 NC_009009:SSA_0513|3end_ATP:cob(I)alamin_adenosyltransferase,_putative_512237..512387_POSITIVE 346 170 122 87 41 NC_009009:SSA_1371|3end_FmtA-like_protein,_putative_1387051..1387201_NEGATIVE 330 143 102 147 107 NC_009009:SSA_0355|3end_DNA_mismatch_repair_protein,_putative_349260..349410_POSITIVE 326 124 81 59 18 NC_009009:SSA_0334|3end_Diphosphomevalonate_decarboxylase,_putative_328882..329032_POSITIVE 316 111 71 151 95 NC_009009:SSA_2330|3end_Acetyltransferase,_putative_2333170..2333320_NEGATIVE 316 120 71 105 45 NC_009009:SSA_1267|3end_crcB-like_protein_2,_putative_1290973..1291123_NEGATIVE 312 118 80 34 1 NC_009009:SSA_0466|3end_Precorrin-8X_methylmutase_/_precorrin_isomerase,_putative_470668..470818_POSITIVE 307 110 62 130 77 NC_009009:SSA_0219|3end_PTS_system,_sugar-specific_enzyme_IIA_component,_putative_215884..216034_POSITIVE 303 144 102 107 68 NC_009009:SSA_0786|3end_Proton-translocating_ATPase,_F1_sector,_alpha_subunit,_putative_770181..770331_POSITIVE 301 139 91 113 65 NC_009009:SSA_0635|3end_Indole-3-glycerol_phosphate_synthase,_putative_625235..625385_POSITIVE 300 108 76 147 109 NC_009009:SSA_0581|3end_Conserved_uncharacterized_protein_573774..573924_POSITIVE 300 130 86 56 1 NC_009009:SSA_0546|3end_Phospho-2-dehydro-3-deoxyheptonate_aldolase_(DAHP_synthatase),_possibly_tyr-sensitive,_putative_541963..542113_POSITIVE 296 130 81 145 97 NC_009009:SSA_1869|3end_ATP-dependent_RNA_helicase,_putative_1864152..1864302_NEGATIVE 295 108 61 147 94 NC_009009:SSA_1789|3end_Metal-dependent_phosphohydrolase,_HD_superfamily,_putative_1772365..1772515_NEGATIVE 295 140 91 103 48 NC_009009:SSA_0537|3end_Propanediol_utilization:dioldehydratase_reactivation,_putative_533893..534043_POSITIVE 294 107 63 132 92 NC_009009:SSA_2164|3end_Glutamine_amidotransferase,_putative_2160866..2161016_NEGATIVE 293 138 96 144 100 NC_009009:SSA_1368|3end_hypothetical_protein_1383748..1383898_NEGATIVE 290 110 61 150 86 NC_009009:SSA_2058|3end_30S_ribosomal_protein_S15,_putative_2060058..2060208_NEGATIVE 287 106 61 150 103 NC_009009:SSA_0980|3end_3-isopropylmalate_dehydratase_large_subunit,_putative_985466..985616_POSITIVE 287 139 105 73 40 NC_009009:SSA_0287|3end_Glycerol_dehydrogenase,_NAD+_dependent,_putative_280702..280852_POSITIVE