Origin IGS: ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctattcctccaatggttttgtcttaggttagacgaagaacactaacctttgttaaatatttcacaattcaattataacttatttaataccttttgtaaacactttcactcaacattcacaaacttttttctattactttgtgaaatacataacaaatttgcttgttttacactctattcttttagttattgtgaaaaaattttttacattttaaagatttcacatacttttactttcacataatatatgaaagcgctttctttgtttgaatataaaaagctcggacgaaccgagctgattat Mask Tandem Repeat Region ================================================ ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Find is-nt database================================================ Query_seq: SSA_0067:SSA_0068|SSA_0067:SSA_0068:Acyltransferase (yrhL-like subfamily of SGNH-hydrolases), putative:Alcohol-acetaldehyde dehydrogenase, putative:->->:69951..70253 303 ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: SSA_0067:SSA_0068|SSA_0067:SSA_0068:Acyltransferase (yrhL-like subfamily of SGNH-hydrolases), putative:Alcohol-acetaldehyde dehydrogenase, putative:->->:69951..70253 303 ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: SSA_0067:SSA_0068|SSA_0067:SSA_0068:Acyltransferase (yrhL-like subfamily of SGNH-hydrolases), putative:Alcohol-acetaldehyde dehydrogenase, putative:->->:69951..70253 303 ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 1 End: 96 M I S S V R P S F L Y S N K E S A F I Y Y V K V K V C E I F K M ............................................................................................................................................................................................................... Protein_Len: 52 Strand: - Start: 92 End: 247 ........................................................................................... F T F F K K V I V L L I S H L V L L N T I Y K V F Y Y F F T Q S H Q T F T N V F P I L Y T I I S N H F M ........................................................ Protein_Len: 60 Strand: - Start: 63 End: 254 .............................................................. T H S I K L I Y F I K E C Y S F S Y L T F C A F K N H I E C L L L F F N T F T S H F H K C F T N F L N Y N F Q S I N L M ................................................. ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 88 Start: 203 End: 232 ..........................................................................................................................................................................................................GTTTACAAAAGGTATTAAATAAGTTATAAT....................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: SSA_0067:SSA_0068|SSA_0067:SSA_0068:Acyltransferase (yrhL-like subfamily of SGNH-hydrolases), putative:Alcohol-acetaldehyde dehydrogenase, putative:->->:69951..70253 303 ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Intra-Species Hit: Count: 1 Min: 1 Max: 303 Len: 303 Subject: NC_009009_SSA_0067_SSA_0068|Acyltransferase (yrhL-like subfamily of SGNH-hydrolases), putative:Alcohol-acetaldehyde dehydrogenase, putative|POSITIVE:POSITIVE|[69951,70253]|303 HSP 1 e-value: 1.0E-171 bit: 601.0 Len: 303 Query Start:1 Query End:303 Subject Strand: POSITIVE Subject Start: 1 Subject End: 303 ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga ataatcagctcggttcgtccgagctttttatattcaaacaaagaaagcgctttcatatattatgtgaaagtaaaagtatgtgaaatctttaaaatgtaaaaaattttttcacaataactaaaagaatagagtgtaaaacaagcaaatttgttatgtatttcacaaagtaatagaaaaaagtttgtgaatgttgagtgaaagtgtttacaaaaggtattaaataagttataattgaattgtgaaatatttaacaaaggttagtgttcttcgtctaacctaagacaaaaccattggaggaataga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAUCAGCUCGGUUCGUCCGAGCUUUUUAUAUUCAAACAAAGAAAGCGCUUUCAUAUAUUAUGUGAAAGUAAAAGUAUGUGAAAUCUUUAAAAUGUAAAAAAUUUUUUCACAAUAACUAAAAGAAUAGAGUGUAAAACAAGCAAAUUUGUUAUGUAUUUCACAAAGUAAUAGAAAAAAGUUUGUGAAUGUUGAGUGAAAGUGUUUACAAAAGGUAUUAAAUAAGUUAUAAUUGAAUUGUGAAAUAUUUAACAAAGGUUAGUGUUCUUCGUCUAACCUAAGACAAAACCAUUGGAGGAAUAGA ......(((((((.....)))))))((((((((((.............((((((((((...))))))))))...(((.(((((((.....................)))))))...))).........))))))))))..(((...(((((((.(((((((((((..((((.........((((((((..((.......))..))))))))..(((..........))).))))..))))))))))).))))))).)))..((((((((((((......))))..........)))))))).. (-68.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUAUUCCUCCAAUGGUUUUGUCUUAGGUUAGACGAAGAACACUAACCUUUGUUAAAUAUUUCACAAUUCAAUUAUAACUUAUUUAAUACCUUUUGUAAACACUUUCACUCAACAUUCACAAACUUUUUUCUAUUACUUUGUGAAAUACAUAACAAAUUUGCUUGUUUUACACUCUAUUCUUUUAGUUAUUGUGAAAAAAUUUUUUACAUUUUAAAGAUUUCACAUACUUUUACUUUCACAUAAUAUAUGAAAGCGCUUUCUUUGUUUGAAUAUAAAAAGCUCGGACGAACCGAGCUGAUUAU ......((......))............((((((((((((........(((((((..((((((((((....((((.........))))....(((((.....................)))))...............))))))))))..)))))))...((......................(((...(((((((....((((((.....))))))))))))).)))....((((((.........)))))).)).))))))))))))..((((..(((((((.....)))))))..)))) (-49.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 284 29 4 230 199 NC_009009:SSA_1617|5end_hypothetical_protein_1620651..1620881_NEGATIVE 257 278 231 183 141 NC_009009:SSA_1218|5end_DNA_repair_protein_radC,_putative_1240459..1240689_NEGATIVE 253 56 20 160 125 NC_009009:SSA_0049|5end_Dihydroxyacetone_kinase_(Dak1),_putative_53612..53842_POSITIVE 246 53 14 214 177 NC_009009:SSA_0688|5end_2,3-bisphosphoglycerate-dependent_phosphoglycerate_mutase,_putative_671360..671590_POSITIVE 243 56 11 126 77 NC_009009:SSA_0786|5end_Proton-translocating_ATPase,_F1_sector,_alpha_subunit,_putative_768737..768967_POSITIVE 243 29 4 210 185 NC_009009:SSA_0191|5end_Adenine-specific_DNA_methylase,_putative_191907..192137_POSITIVE 236 50 7 48 8 NC_009009:SSA_2190|5end_33_kDa_chaperonin,_putative_2191166..2191396_POSITIVE 233 43 7 57 12 NC_009009:SSA_1405|5end_Copper_homeostasis_protein_CutC,_putative_1419393..1419623_NEGATIVE 233 50 4 127 87 NC_009009:SSA_0203|5end_hypothetical_protein_201322..201552_POSITIVE 229 34 5 38 4 NC_009009:SSA_2369|5end_hypothetical_protein_2371093..2371323_NEGATIVE 229 57 14 147 105 NC_009009:SSA_0767|5end_Diacylglycerol_kinase_catalytic_domain_protein,_putative_747087..747317_POSITIVE 229 48 6 205 154 NC_009009:SSA_0722|5end_hypothetical_protein_706875..707105_POSITIVE 227 28 1 229 201 NC_009009:SSA_1443|5end_1-(5-phosphoribosyl)-5-[(5-_phosphoribosylamino)methylideneamino]_imidazole-4-carboxamide_isomerase,_putative_1446672..1446902_NEGATIVE 227 25 7 57 36 NC_009009:SSA_0865|5end_hypothetical_protein_856378..856608_POSITIVE 227 50 4 164 115 NC_009009:SSA_0752|5end_hypothetical_protein_734171..734401_POSITIVE 225 299 255 193 143 NC_009009:SSA_0579|5end_Hydrolase,_HAD_superfamily,_putative_572220..572450_POSITIVE 223 278 246 99 64 NC_009009:SSA_0418|5end_AraC-type_DNA-binding_domain-containing_protein,_putative_415256..415486_POSITIVE 223 29 5 58 30 NC_009009:SSA_2393|5end_Transcriptional_regulator,_XRE_family,_putative_204056..204286_POSITIVE 222 279 234 147 100 NC_009009:SSA_1370|5end_CAAX_amino_terminal_protease_family,_putative_1386968..1387198_NEGATIVE 222 57 11 58 3 NC_009009:SSA_0378|5end_TRZ/ATZ_family_hydrolase,_putative_367276..367506_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 267 39 7 100 63 NC_009009:SSA_0343|3end_DNA-damage-inducible_protein_P,_putative_338669..338819_POSITIVE 257 278 231 60 18 NC_009009:SSA_1219|3end_Sortase,_putative_1240582..1240732_NEGATIVE 246 53 14 64 27 NC_009009:SSA_0687|3end_hypothetical_protein_671290..671440_POSITIVE 243 29 4 128 103 NC_009009:SSA_0190|3end_hypothetical_protein_191905..192055_POSITIVE 236 25 7 84 66 NC_009009:SSA_0067|3end_Acyltransferase_(yrhL-like_subfamily_of_SGNH-hydrolases),_putative_69890..70040_POSITIVE 234 48 1 57 4 NC_009009:SSA_0676|3end_Farnesyl_diphosphate_synthase,_putative_659553..659703_POSITIVE 233 50 4 127 87 NC_009009:SSA_0202|3end_hypothetical_protein_201402..201552_POSITIVE 229 48 6 150 99 NC_009009:SSA_0721|3end_Mn/Fe-dependent_superoxide_dismutase,_putative_706900..707050_POSITIVE 224 295 258 124 91 NC_009009:SSA_0312|3end_Hydrolase,_metallo-beta-lactamase_superfamily,_putative_306703..306853_NEGATIVE 222 279 234 64 17 NC_009009:SSA_1371|3end_FmtA-like_protein,_putative_1387051..1387201_NEGATIVE 222 51 11 54 7 NC_009009:SSA_1224|3end_hypothetical_protein_1247251..1247401_NEGATIVE 220 296 266 41 11 NC_009009:SSA_0590|3end_hypothetical_protein_582817..582967_POSITIVE 218 29 2 113 82 NC_009009:SSA_1036|3end_Deoxyribose-phosphate_aldolase,_putative_1052211..1052361_POSITIVE 217 50 4 142 90 NC_009009:SSA_0466|3end_Precorrin-8X_methylmutase_/_precorrin_isomerase,_putative_470668..470818_POSITIVE 215 31 7 69 44 NC_009009:SSA_2200|3end_Firmicutes_transcriptional_repressor_of_class_III_stress_genes_(CtsR),_putative_2201109..2201259_NEGATIVE 213 49 4 135 90 NC_009009:SSA_1490|3end_Ribonuclease_BN,_putative_1495821..1495971_NEGATIVE 213 48 1 66 10 NC_009009:SSA_1032|3end_30S_ribosomal_protein_subunit_S20,_putative_1048313..1048463_NEGATIVE 211 297 265 43 9 NC_009009:SSA_1934|3end_Biotin_carboxyl_carrier_protein_of_acetyl-CoA_carboxylase,_putative_1921312..1921462_NEGATIVE 211 297 254 142 87 NC_009009:SSA_0607|3end_ABC_transporter,_permease_component,_putative_594590..594740_NEGATIVE 210 51 17 53 17 NC_009009:SSA_2367|3end_ABC-type_cobalt_transport_system,_ATPase_component,_putative_2368845..2368995_NEGATIVE