Origin IGS: ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttaaataaaatttagaggggattatttttataatcccattttttgtttataaataattaatatctaaaaatactaggattatttatgttaaaggagagtaaattttatttacaggtattattgaaattaagaataataatagtaaacttcaagttttagctgaaattaaaaatggtaaattaattaagaaacttatcaaaatggaaataaaatttaaatataattattttataatagatttttaccaaatattaataaaagcctgatagaatataattaacatttaaagatattaggggtgatgag Mask Tandem Repeat Region ================================================ ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Find is-nt database================================================ Query_seq: FN2119:FN2120|FN2119:FN2120:Hypothetical exported 24-amino acid repeat protein:Hypothetical exported 24-amino acid repeat protein:<-<-:628143..628450 308 ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN2119:FN2120|FN2119:FN2120:Hypothetical exported 24-amino acid repeat protein:Hypothetical exported 24-amino acid repeat protein:<-<-:628143..628450 308 ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN2119:FN2120|FN2119:FN2120:Hypothetical exported 24-amino acid repeat protein:Hypothetical exported 24-amino acid repeat protein:<-<-:628143..628450 308 ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: - Start: 44 End: 205 ........................................... A K I L I Q Y F D I I F Y N Y K F K I E M K I L K K I L K G N K I E A L V Q L K S N N N K I E I I G T F L M ....................................................................................................... ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN2119:FN2120|FN2119:FN2120:Hypothetical exported 24-amino acid repeat protein:Hypothetical exported 24-amino acid repeat protein:<-<-:628143..628450 308 ctcatcacccctaatatctttaaatgttaattatattctatcaggcttttattaatatttggtaaaaatctattataaaataattatatttaaattttatttccattttgataagtttcttaattaatttaccatttttaatttcagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttagatattaattatttataaacaaaaaatgggattataaaaataatcccctctaaattttatttaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 243 Len: 243 Subject: NC_003454_FN2119_FN2120|Hypothetical exported 24-amino acid repeat protein:Hypothetical exported 24-amino acid repeat protein|NEGATIVE:NEGATIVE|[628143,628450]|308 HSP 1 e-value: 3.0E-49 bit: 196.0 Len: 99 Query Start:145 Query End:243 Subject Strand: POSITIVE Subject Start: 145 Subject End: 243 ................................................................................................................................................cagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttag................................................................. ................................................................................................................................................cagctaaaacttgaagtttactattattattcttaatttcaataatacctgtaaataaaatttactctcctttaacataaataatcctagtatttttag................................................................. HSP 2 e-value: 1.0E-17 bit: 91.7 Len: 46 Query Start:1 Query End:46 Subject Strand: POSITIVE Subject Start: 1 Subject End: 46 ctcatcacccctaatatctttaaatgttaattatattctatcaggc...................................................................................................................................................................................................................................................................... ctcatcacccctaatatctttaaatgttaattatattctatcaggc...................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUCAUCACCCCUAAUAUCUUUAAAUGUUAAUUAUAUUCUAUCAGGCUUUUAUUAAUAUUUGGUAAAAAUCUAUUAUAAAAUAAUUAUAUUUAAAUUUUAUUUCCAUUUUGAUAAGUUUCUUAAUUAAUUUACCAUUUUUAAUUUCAGCUAAAACUUGAAGUUUACUAUUAUUAUUCUUAAUUUCAAUAAUACCUGUAAAUAAAAUUUACUCUCCUUUAACAUAAAUAAUCCUAGUAUUUUUAG .......................(((((((.............((((...(((((....(((((((.......(((((.....))))).......((((((........))))))............)))))))...)))))...))))..........((((((...(((((((.........)))))))...))))))...............)))))))..................... (-24.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CUAAAAAUACUAGGAUUAUUUAUGUUAAAGGAGAGUAAAUUUUAUUUACAGGUAUUAUUGAAAUUAAGAAUAAUAAUAGUAAACUUCAAGUUUUAGCUGAAAUUAAAAAUGGUAAAUUAAUUAAGAAACUUAUCAAAAUGGAAAUAAAAUUUAAAUAUAAUUAUUUUAUAAUAGAUUUUUACCAAAUAUUAAUAAAAGCCUGAUAGAAUAUAAUUAACAUUUAAAGAUAUUAGGGGUGAUGAG .......(((((.....((((..((((((((((.((((((...)))))).(.((((((((..........)))))))).)...))))....))))))..))))......)))))..............(((((((...(((....((((((((..(((((.....))))).))))))))..)))..............(((((((...(.(((.......))).)..)))))))..))))))) (-31.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 747 50 1 141 92 NC_003454:FN2119|5end_Hypothetical_exported_24-amino_acid_repeat_protein_628052..628282_NEGATIVE 388 140 93 163 112 NC_003454:FN2118|5end_Hypothetical_exported_24-amino_acid_repeat_protein_627024..627254_NEGATIVE 293 140 94 176 126 NC_003454:FN2117|5end_Hypothetical_exported_24-amino_acid_repeat_protein_626259..626489_NEGATIVE 268 200 161 41 2 NC_003454:FN2110|5end_Hypothetical_exported_24-amino_acid_repeat_protein_622330..622560_NEGATIVE 236 150 114 45 8 NC_003454:FN0465|5end_hypothetical_protein_1110046..1110276_POSITIVE 233 181 141 83 42 NC_003454:FN1125|5end_LemA_protein_1769811..1770041_NEGATIVE 219 173 133 65 29 NC_003454:FN0683|5end_hypothetical_protein_1338759..1338989_NEGATIVE 211 113 88 178 153 NC_003454:FN0587|5end_Hypothetical_membrane-spanning_protein_1242139..1242369_NEGATIVE 209 139 92 61 1 NC_003454:FN0627|5end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing]_1287165..1287395_NEGATIVE 208 43 1 142 87 NC_003454:FN1996|5end_Transporter_498955..499185_POSITIVE 207 166 122 68 29 NC_003454:FN0066|5end_Two_component_system_histidine_kinase_702327..702557_POSITIVE 206 179 136 90 40 NC_003454:FN0784|5end_Electron_transfer_flavoprotein_beta-subunit_1440332..1440562_POSITIVE 202 217 175 79 27 NC_003454:FN0959|5end_Precorrin-2_C20-methyltransferase_1615058..1615288_NEGATIVE 201 164 127 142 97 NC_003454:FN1275|5end_Acriflavin_resistance_protein_B_1931725..1931955_POSITIVE 200 150 107 90 39 NC_003454:FN1756|5end_ThiS_protein_251935..252165_NEGATIVE 198 50 5 138 96 NC_003454:FN2020|5end_translation_initiation_factor_IF-2_524522..524752_NEGATIVE 195 148 101 52 7 NC_003454:FN2058|5end_Fusobacterium_outer_membrane_protein_family_571095..571325_NEGATIVE 194 139 95 95 55 NC_003454:FN1392|5end_SSU_ribosomal_protein_S16P_2049459..2049689_NEGATIVE 194 178 131 98 47 NC_003454:FN0679|5end_GTPase_1334596..1334826_POSITIVE 193 149 101 188 128 NC_003454:FN1909|5end_UDP-3-O-[3-hydroxymyristoyl]_glucosamine_N-acyltransferase_402710..402940_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 388 140 93 151 100 NC_003454:FN2119|3end_Hypothetical_exported_24-amino_acid_repeat_protein_627036..627186_NEGATIVE 293 140 94 148 98 NC_003454:FN2118|3end_Hypothetical_exported_24-amino_acid_repeat_protein_626287..626437_NEGATIVE 233 181 141 74 33 NC_003454:FN1126|3end_Acetyltransferase_1769820..1769970_NEGATIVE 216 66 24 66 21 NC_003454:FN1872|3end_hypothetical_protein_362789..362939_NEGATIVE 215 218 171 65 18 NC_003454:FN1937|3end_hypothetical_protein_437705..437855_NEGATIVE 214 210 173 141 108 NC_003454:FN0865|3end_hypothetical_protein_1523040..1523190_NEGATIVE 211 113 88 148 123 NC_003454:FN0588|3end_hypothetical_protein_1242169..1242319_NEGATIVE 208 43 1 145 90 NC_003454:FN1995|3end_hypothetical_protein_499038..499188_POSITIVE 206 179 136 69 19 NC_003454:FN0783|3end_Acyl-CoA_dehydrogenase_1440391..1440541_POSITIVE 201 164 127 142 97 NC_003454:FN1274|3end_Acriflavin_resistance_protein_E_1931805..1931955_POSITIVE 200 140 94 148 93 NC_003454:FN2110|3end_Hypothetical_exported_24-amino_acid_repeat_protein_621908..622058_NEGATIVE 200 150 107 77 26 NC_003454:FN1757|3end_thiamine_biosynthesis_protein_ThiC_251948..252098_NEGATIVE 198 50 5 111 69 NC_003454:FN2021|3end_ABC1_family_protein_524549..524699_NEGATIVE 194 139 95 44 4 NC_003454:FN1393|3end_Signal_recognition_particle,_subunit_FFH/SRP54_2049510..2049660_NEGATIVE 194 178 131 73 22 NC_003454:FN0678|3end_Ser/Thr_protein_kinase_1334651..1334801_POSITIVE 187 170 129 142 95 NC_003454:FN0955|3end_hypothetical_protein_1611928..1612078_NEGATIVE 187 47 8 135 100 NC_003454:FN0771|3end_Oxygen-independent_coproporphyrinogen_III_oxidase_1428777..1428927_POSITIVE 186 70 24 68 18 NC_003454:FN0273|3end_Butyrate-acetoacetate_CoA-transferase_subunit_B_900162..900312_POSITIVE 182 240 197 61 16 NC_003454:FN0285|3end_RNA_binding_protein_913932..914082_NEGATIVE 181 237 191 64 12 NC_003454:FN0311|3end_anaerobic_ribonucleoside_triphosphate_reductase_949391..949541_POSITIVE