Origin IGS: aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atgaaagagttgaagtataagagtttttgttgggtaattggaactacaagttttcgtacagcaaaattgaatttaaaaatagaacaacaattaagattgttaaatgaatttcataaaaacataaatccttgggaatggactaattctactcaggaaaaatattatgatttt Mask Tandem Repeat Region ================================================ aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Find is-nt database================================================ Query_seq: FN1922:FN1923|FN1922:FN1923:hypothetical protein:Adenine-specific methyltransferase:<-<-:419526..419696 171 aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1922:FN1923|FN1922:FN1923:hypothetical protein:Adenine-specific methyltransferase:<-<-:419526..419696 171 aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1922:FN1923|FN1922:FN1923:hypothetical protein:Adenine-specific methyltransferase:<-<-:419526..419696 171 aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 36 End: 125 ................................... M P K D L C F Y E I H L T I L I V V L F L N S I L L Y E N L .............................................. Protein_Len: 57 Strand: - Start: 1 End: 171 F D Y Y K E Q T S N T W E W P N I N K H F E N L L R L Q Q E I K L N L K A T R F S T T G I V W C F S K Y K L E K M aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN1922:FN1923|FN1922:FN1923:hypothetical protein:Adenine-specific methyltransferase:<-<-:419526..419696 171 aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Intra-Species Hit: Count: 1 Min: 1 Max: 171 Len: 171 Subject: NC_003454_FN1922_FN1923|hypothetical protein:Adenine-specific methyltransferase|NEGATIVE:NEGATIVE|[419526,419696]|171 HSP 1 e-value: 2.0E-92 bit: 339.0 Len: 171 Query Start:1 Query End:171 Subject Strand: POSITIVE Subject Start: 1 Subject End: 171 aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat aaaatcataatatttttcctgagtagaattagtccattcccaaggatttatgtttttatgaaattcatttaacaatcttaattgttgttctatttttaaattcaattttgctgtacgaaaacttgtagttccaattacccaacaaaaactcttatacttcaactctttcat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAUCAUAAUAUUUUUCCUGAGUAGAAUUAGUCCAUUCCCAAGGAUUUAUGUUUUUAUGAAAUUCAUUUAACAAUCUUAAUUGUUGUUCUAUUUUUAAAUUCAAUUUUGCUGUACGAAAACUUGUAGUUCCAAUUACCCAACAAAAACUCUUAUACUUCAACUCUUUCAU ....................((((.(((..(((((........)))))..((((...(((.....)))..)))).......((((((..................(((((.....)))))....(((((....))))).))))))............))).))))...... (-18.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUGAAAGAGUUGAAGUAUAAGAGUUUUUGUUGGGUAAUUGGAACUACAAGUUUUCGUACAGCAAAAUUGAAUUUAAAAAUAGAACAACAAUUAAGAUUGUUAAAUGAAUUUCAUAAAAACAUAAAUCCUUGGGAAUGGACUAAUUCUACUCAGGAAAAAUAUUAUGAUUUU ((((((..(((.............((((((((....((..((((.....))))..)).))))))))...................((((((....)))))).)))...)))))).....(((((.((((..(((((......)))))....))))......)))))..... (-22.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 756 60 11 151 102 NC_003454:FN1922|5end_hypothetical_protein_419435..419665_NEGATIVE 236 67 21 230 184 NC_003454:FN0254|5end_Fusobacterium_outer_membrane_protein_family_876277..876507_POSITIVE 233 133 100 227 199 NC_003454:FN1540|5end_Iron-sulfur_cluster-binding_protein_53055..53285_POSITIVE 232 70 22 83 32 NC_003454:FN1918|5end_SPO0B-associated_GTP-binding_protein_415026..415256_NEGATIVE 232 141 107 163 130 NC_003454:FN1623|5end_Ribosome_Recycling_Factor_(RRF)_130394..130624_POSITIVE 230 59 19 91 46 NC_003454:FN1931|5end_Protease_432421..432651_NEGATIVE 225 128 88 36 2 NC_003454:FN1472|5end_N-acetylneuraminate-binding_protein_2154025..2154255_POSITIVE 216 126 82 229 181 NC_003454:FN0883|5end_hypothetical_protein_1544357..1544587_POSITIVE 207 150 102 77 27 NC_003454:FN0508|5end_Serine_protease_1158652..1158882_NEGATIVE 205 68 33 209 176 NC_003454:FN0862|5end_hypothetical_protein_1522371..1522601_NEGATIVE 203 114 76 82 49 NC_003454:FN1329|5end_Methyltransferase_1983890..1984120_NEGATIVE 203 133 99 217 184 NC_003454:FN0225|5end_Gluconate_permease_853073..853303_NEGATIVE 200 58 18 205 167 NC_003454:FN0887|5end_Oligoendopeptidase_F_1549711..1549941_NEGATIVE 200 57 14 190 146 NC_003454:FN2075|5end_hypothetical_protein_587369..587599_NEGATIVE 200 153 119 230 196 NC_003454:FN1629|5end_50S_ribosomal_protein_L6_134508..134738_NEGATIVE 199 145 101 80 31 NC_003454:FN0683|5end_hypothetical_protein_1338759..1338989_NEGATIVE 196 116 73 185 137 NC_003454:FN1737|5end_V-type_ATP_synthase_subunit_F_236356..236586_NEGATIVE 194 169 121 170 126 NC_003454:FN1210|5end_Metal_dependent_hydrolase_1870766..1870996_NEGATIVE 193 55 12 50 11 NC_003454:FN0127|5end_Fe-S_oxidoreductase_756312..756542_NEGATIVE 192 50 3 115 58 NC_003454:FN0284|5end_16S_rRNA_processing_protein_rimM_913923..914153_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 232 141 107 128 95 NC_003454:FN1622|3end_Uridylate_kinase_130439..130589_POSITIVE 230 59 19 94 49 NC_003454:FN1932|3end_Dephospho-CoA_kinase_432418..432568_NEGATIVE 206 132 91 120 86 NC_003454:FN1364|3end_LSU_ribosomal_protein_L32P_2015382..2015532_NEGATIVE 203 114 76 69 36 NC_003454:FN1330|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_1983903..1984053_NEGATIVE 192 50 3 106 49 NC_003454:FN0285|3end_RNA_binding_protein_913932..914082_NEGATIVE 190 139 101 150 115 NC_003454:FN2107|3end_galactokinase_619399..619549_POSITIVE 190 47 4 44 1 NC_003454:FN1636|3end_SSU_ribosomal_protein_S17P_136601..136751_NEGATIVE 188 140 108 68 34 NC_003454:FN1538|3end_Arsenical_pump-driving_ATPase_51109..51259_NEGATIVE 186 97 53 115 67 NC_003454:FN1577|3end_Rod_shape-determining_protein_mreB_87377..87527_NEGATIVE 185 159 112 62 8 NC_003454:FN1366|3end_Triosephosphate_isomerase_2016763..2016913_NEGATIVE 185 132 94 79 43 NC_003454:FN0215|3end_MGTC/SAPB_Family_membrane_protein_839240..839390_NEGATIVE 182 59 13 76 35 NC_003454:FN1455|3end_UDP-N-acetylenolpyruvoylglucosamine_reductase_2135674..2135824_NEGATIVE 177 170 121 106 49 NC_003454:FN1993|3end_SUA5_protein_497650..497800_POSITIVE 174 140 92 64 21 NC_003454:FN0260|3end_Transcriptional_repressor_smtB_886261..886411_NEGATIVE 172 139 102 50 12 NC_003454:FN0464|3end_hypothetical_protein_1110087..1110237_POSITIVE 170 114 85 111 74 NC_003454:FN0238|3end_hypothetical_protein_862382..862532_NEGATIVE 170 168 127 117 75 NC_003454:FN1864|3end_DNA_mismatch_repair_protein_mutS_354678..354828_NEGATIVE 168 57 20 55 14 NC_003454:FN1239|3end_hypothetical_protein_1896403..1896553_NEGATIVE 168 123 88 41 2 NC_003454:FN2119|3end_Hypothetical_exported_24-amino_acid_repeat_protein_627036..627186_NEGATIVE 167 56 13 62 19 NC_003454:FN2127|3end_hypothetical_protein_638226..638376_NEGATIVE