Origin IGS: tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaaaaataatttagattaatataaaaatgatattgagggaaaaaattttatcaatatcattttttattaaaaattaatatatttcttgtataaaattagtaaaaacgttataatgtttaatagatataatttagaatagcttatatgaaagaggcagtacataatgttgaattcaataaaaaaaattcctaaaaaaatctcaatacctgtatctatagttgcagtaatagtctttattattactgctgttttgcttaacttagaaaaaataattgaaaaagtaagtgccagatttataaatggtagagttgttattgagaatgttgatttatcgttttcaaaagctattgtgaaaaacataacactatatgatgataaaaataatgtattgtttaattcaccagaagttattgccaatatcagttttaaaaatttaaaaaagggaagaatagatgagttagtagtgaattcagctgttgtaaatgtaattagagataaagatggagtaataaatttcactaaactatctaaaactaaaagtgaagaaaaacctaaaaatccacttaataaagtggtagcttctaatgtcagagtaaattatgaagactacacttttcctagtaaacttgaaagaaagatagaaaatattaatgctattgtaactgcaagtaaagaaaagttagttgaaacagcagatatcaatatagaggatgaaaatatacaattaaaaactttttttaaagatgaaagtaatgataaaattacttctttacaaggggagttaagaatagataaatttttacttgataaagacttattaaaaagtcttgttaataataaaaaaatgtatttttcagatgtaaatataatttcagatttatcctttaaaactgataaaactatgaaaaatacgaatatagttgggaatttagatataatttcagaattttttagatataatgatatagatagtgatattaaagatattaaattgtctagtaaattcaatggtagagatggagaagcaaacttaggattaaatatatttggaaagaatagagatttttctttagtatataaagatgaagaattaaattcagtaataaca Mask Tandem Repeat Region ================================================ tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Find is-nt database================================================ Query_seq: FN1912:FN1913|FN1912:FN1913:hypothetical protein:hydrolase (HD superfamily):<-<-:409036..410143 1108 tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1912:FN1913|FN1912:FN1913:hypothetical protein:hydrolase (HD superfamily):<-<-:409036..410143 1108 tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1912:FN1913|FN1912:FN1913:hypothetical protein:hydrolase (HD superfamily):<-<-:409036..410143 1108 tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 9 End: 101 ........ M N L I L H L Y I L K K N L Y S F Q I Y L I L S L L L H L Y H ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 5 End: 499 .... M T E F N S S S L Y T K E K S L F F P N I F N P K F A S P S L P L N L L D N L I S L I S L S I S L Y L K N S E I I S K F ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 42 Strand: + Start: 457 End: 582 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................ M N I F Y L S F K F T R K S V V F I I Y S D I R S Y H F I K W I F R F F F T F S F R .............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 518 End: 1039 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M T L E A T T L L S G F L G F S S L L V L D S L V K F I T P S L S L I T F T T A E F T T N S S I L P F F K F L K L I L A ..................................................................... Protein_Len: 60 Strand: - Start: 1 End: 948 S F S L D V N E I V V R G N I F R A S V K E I I K E L N L L V A T I I F V I V A V I S V P I S I K K P I K K I S N L M M ................................................................................................................................................................ tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 995 End: 1024 ..................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTTGTATAAAATTAGTAAAAACGTTATAAT.................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 70 HP_score: -7.6 Tail_Score: -3.53717 Start: 534 End: 549 Full_Region: TGACATTAGAAGCTA CCACTT TATT AAGTGG ATTTTTAGGTTTTTC .....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CCACTTTATTAAGTGG............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 88 HP_score: -9.9 Tail_Score: -3.82923 Start: 863 End: 888 Full_Region: cctgtatctatagtt gcagtaatagt cttt attattactgc tgttttgcttaactt ..............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gcagtaatagtctttattattactgc............................................................................................................................................................................................................................ Find igs database================================================ Query_seq: FN1912:FN1913|FN1912:FN1913:hypothetical protein:hydrolase (HD superfamily):<-<-:409036..410143 1108 tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccactttattaagtggatttttaggtttttcttcacttttagttttagatagtttagtgaaatttattactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgagatttttttaggaattttttttattgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacgtttttactaattttatacaagaaatatattaatttttaataaaaaatgatattgataaaattttttccctcaatatcatttttatattaatctaaattatttttt Intra-Species Hit: Count: 1 Min: 1 Max: 1003 Len: 1003 Subject: NC_003454_FN1912_FN1913|hypothetical protein:hydrolase (HD superfamily)|NEGATIVE:NEGATIVE|[409036,410143]|1108 HSP 1 e-value: 0.0 bit: 981.0 Len: 537 Query Start:1 Query End:537 Subject Strand: POSITIVE Subject Start: 1 Subject End: 537 tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacannnnnnnattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttnnnnnnngtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccac........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... tgttattactgaatttaattcttcatctttatatactaaagaaaaatctctattctttccaaatatatttaatcctaagtttgcttctccatctctaccattgaatttactagacaatttaatatctttaatatcactatctatatcattatatctaaaaaattctgaaattatatctaaattcccaactatattcgtatttttcatagttttatcagttttaaaggataaatctgaaattatatttacatctgaaaaatacatttttttattattaacaagactttttaataagtctttatcaagtaaaaatttatctattcttaactccccttgtaaagaagtaattttatcattactttcatctttaaaaaaagtttttaattgtatattttcatcctctatattgatatctgctgtttcaactaacttttctttacttgcagttacaatagcattaatattttctatctttctttcaagtttactaggaaaagtgtagtcttcataatttactctgacattagaagctaccac........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-176 bit: 618.0 Len: 312 Query Start:600 Query End:911 Subject Strand: POSITIVE Subject Start: 600 Subject End: 911 .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgaga..................................................................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ttactccatctttatctctaattacatttacaacagctgaattcactactaactcatctattcttcccttttttaaatttttaaaactgatattggcaataacttctggtgaattaaacaatacattatttttatcatcatatagtgttatgtttttcacaatagcttttgaaaacgataaatcaacattctcaataacaactctaccatttataaatctggcacttactttttcaattattttttctaagttaagcaaaacagcagtaataataaagactattactgcaactatagatacaggtattgaga..................................................................................................................................................................................................... HSP 3 e-value: 2.0E-32 bit: 143.0 Len: 72 Query Start:932 Query End:1003 Subject Strand: POSITIVE Subject Start: 932 Subject End: 1003 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................attgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacg......................................................................................................... ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................attgaattcaacattatgtactgcctctttcatataagctattctaaattatatctattaaacattataacg......................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUUAUUACUGAAUUUAAUUCUUCAUCUUUAUAUACUAAAGAAAAAUCUCUAUUCUUUCCAAAUAUAUUUAAUCCUAAGUUUGCUUCUCCAUCUCUACCAUUGAAUUUACUAGACAAUUUAAUAUCUUUAAUAUCACUAUCUAUAUCAUUAUAUCUAAAAAAUUCUGAAAUUAUAUCUAAAUUCCCAACUAUAUUCGUAUUUUUCAUAGUUUUAUCAGUUUUAAAGGAUAAAUCUGAAAUUAUAUUUACAUCUGAAAAAUACAUUUUUUUAUUAUUAACAAGACUUUUUAAUAAGUCUUUAUCAAGUAAAAAUUUAUCUAUUCUUAACUCCCCUUGUAAAGAAGUAAUUUUAUCAUUACUUUCAUCUUUAAAAAAAGUUUUUAAUUGUAUAUUUUCAUCCUCUAUAUUGAUAUCUGCUGUUUCAACUAACUUUUCUUUACUUGCAGUUACAAUAGCAUUAAUAUUUUCUAUCUUUCUUUCAAGUUUACUAGGAAAAGUGUAGUCUUCAUAAUUUACUCUGACAUUAGAAGCUACCACUUUAUUAAGUGGAUUUUUAGGUUUUUCUUCACUUUUAGUUUUAGAUAGUUUAGUGAAAUUUAUUACUCCAUCUUUAUCUCUAAUUACAUUUACAACAGCUGAAUUCACUACUAACUCAUCUAUUCUUCCCUUUUUUAAAUUUUUAAAACUGAUAUUGGCAAUAACUUCUGGUGAAUUAAACAAUACAUUAUUUUUAUCAUCAUAUAGUGUUAUGUUUUUCACAAUAGCUUUUGAAAACGAUAAAUCAACAUUCUCAAUAACAACUCUACCAUUUAUAAAUCUGGCACUUACUUUUUCAAUUAUUUUUUCUAAGUUAAGCAAAACAGCAGUAAUAAUAAAGACUAUUACUGCAACUAUAGAUACAGGUAUUGAGAUUUUUUUAGGAAUUUUUUUUAUUGAAUUCAACAUUAUGUACUGCCUCUUUCAUAUAAGCUAUUCUAAAUUAUAUCUAUUAAACAUUAUAACG .(((((...((((((((((......((((((.....))))))..............................(((((((......((((..........((((((((........))))))))......((((((..((((((((...........(((((((..(((((........................((((...(((((.((((.((((((((((((((((..(((..((...............(((((((((((..((((((((......(((((((......))))))).....))))))))....................(((.((((((((((((......))))))....))))))....)))........))))).))))))..........(((..((.((((((...(((((((((((((.(((((........((((.(........).))))........))))).....))))))))).)))).(((((...(((.(((((.((((((((...((((((....))))))......(((....)))...)))))))).))))))))....)))))....................................)))))).))..)))...........)).)))..)))............)))))))))))))................(((((..(((((..(((((((............)))))))..))))))))))...))))..)))))))))............................(((..........))).........)))))...))))))).................(((((((((........)))))))))...))))))))..))))))))))....))))))).........)))))))))).....................((((((...........))))))............))))). (-148.49) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGUUAUAAUGUUUAAUAGAUAUAAUUUAGAAUAGCUUAUAUGAAAGAGGCAGUACAUAAUGUUGAAUUCAAUAAAAAAAAUUCCUAAAAAAAUCUCAAUACCUGUAUCUAUAGUUGCAGUAAUAGUCUUUAUUAUUACUGCUGUUUUGCUUAACUUAGAAAAAAUAAUUGAAAAAGUAAGUGCCAGAUUUAUAAAUGGUAGAGUUGUUAUUGAGAAUGUUGAUUUAUCGUUUUCAAAAGCUAUUGUGAAAAACAUAACACUAUAUGAUGAUAAAAAUAAUGUAUUGUUUAAUUCACCAGAAGUUAUUGCCAAUAUCAGUUUUAAAAAUUUAAAAAAGGGAAGAAUAGAUGAGUUAGUAGUGAAUUCAGCUGUUGUAAAUGUAAUUAGAGAUAAAGAUGGAGUAAUAAAUUUCACUAAACUAUCUAAAACUAAAAGUGAAGAAAAACCUAAAAAUCCACUUAAUAAAGUGGUAGCUUCUAAUGUCAGAGUAAAUUAUGAAGACUACACUUUUCCUAGUAAACUUGAAAGAAAGAUAGAAAAUAUUAAUGCUAUUGUAACUGCAAGUAAAGAAAAGUUAGUUGAAACAGCAGAUAUCAAUAUAGAGGAUGAAAAUAUACAAUUAAAAACUUUUUUUAAAGAUGAAAGUAAUGAUAAAAUUACUUCUUUACAAGGGGAGUUAAGAAUAGAUAAAUUUUUACUUGAUAAAGACUUAUUAAAAAGUCUUGUUAAUAAUAAAAAAAUGUAUUUUUCAGAUGUAAAUAUAAUUUCAGAUUUAUCCUUUAAAACUGAUAAAACUAUGAAAAAUACGAAUAUAGUUGGGAAUUUAGAUAUAAUUUCAGAAUUUUUUAGAUAUAAUGAUAUAGAUAGUGAUAUUAAAGAUAUUAAAUUGUCUAGUAAAUUCAAUGGUAGAGAUGGAGAAGCAAACUUAGGAUUAAAUAUAUUUGGAAAGAAUAGAGAUUUUUCUUUAGUAUAUAAAGAUGAAGAAUUAAAUUCAGUAAUAACA .(((((...((((..((((((..(((((.((((((.....((((..(((((((((((..(((((....)))))...................((((((((.(....(((((....(((((((((((....)))))))))))......(((((.(((.................))))))))................)))))...).)))))))).......(((((((((............(((.....)))...........)))))))))....)))))))))))..))))...(((.(((((((.((((((.(((((..................))))).)))..))).))))))).))).)))))).)))))..............((.((((((.....))))))))....))))))))))....(.((((......(((((....((((((....))))))..((((((.((.((...(.((((((((..(((((.(((((((.......(((((.......(((((...((....)).))))).......)))))...))))))))))))....((((...(((((...(((((((((((..............(((((((((((((((....((((((......))))))))))))..)))))))))..(((.(((((..((((((.(((((((.(((((......))))))))))))...))))))....)))))))).....................)))))))))))....)))))....(((.....)))........))))..........))))))))).((((((.(((((((...((((((.((((....))))....))))))...))))))).)))))).......)).))..))))))....))))).....(((((((.(((((((.......)))))))..))))))).................)))).).))))). (-189.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 791 120 71 211 162 NC_003454:FN1912|5end_hypothetical_protein_408945..409175_NEGATIVE 304 96 51 136 95 NC_003454:FN0940|5end_hypothetical_protein_1593508..1593738_NEGATIVE 295 689 643 129 76 NC_003454:FN0201|5end_Glutaconyl-COA_decarboxylase_beta_subunit_826542..826772_POSITIVE 288 114 74 194 157 NC_003454:FN1126|5end_Acetyltransferase_1770287..1770517_NEGATIVE 279 573 531 214 177 NC_003454:FN0698|5end_DNA_integration/recombination/invertion_protein_1356611..1356841_POSITIVE 264 470 427 39 4 NC_003454:FN0248|5end_hypothetical_protein_871562..871792_POSITIVE 263 668 625 113 62 NC_003454:FN0270|5end_GTP-binding_protein_era_896770..897000_POSITIVE 262 484 443 83 36 NC_003454:FN0082|5end_Ethanolamine_utilization_protein_eutM_720699..720929_POSITIVE 261 519 473 102 36 NC_003454:FN2022|5end_transcription_elongation_factor_NusA_526145..526375_NEGATIVE 259 679 633 98 43 NC_003454:FN1008|5end_hypothetical_protein_1663067..1663297_NEGATIVE 257 111 78 86 50 NC_003454:FN0508|5end_Serine_protease_1158652..1158882_NEGATIVE 256 442 403 107 65 NC_003454:FN1500|5end_Nickel_transport_ATP-binding_protein_nikE_5927..6157_NEGATIVE 253 590 541 216 164 NC_003454:FN1490|5end_4-hydroxybenzoate_octaprenyltransferase_2172283..2172513_NEGATIVE 253 440 392 107 53 NC_003454:FN0906|5end_Glycerol-3-phosphate_dehydrogenase_[NAD(P)+]_1565131..1565361_NEGATIVE 251 477 431 98 48 NC_003454:FN1108|5end_Phosphohydrolase_(MUTT/NUDIX_family_protein)_1752310..1752540_POSITIVE 249 583 545 200 153 NC_003454:FN0200|5end_Biotin_carboxyl_carrier_protein_of_glutaconyl-COA_decarboxylase_826123..826353_POSITIVE 245 878 833 173 121 NC_003454:FN0936|5end_CapC_protein_1589107..1589337_NEGATIVE 245 589 550 179 139 NC_003454:FN2008|5end_Glycine_betaine_transport_ATP-binding_protein_508319..508549_NEGATIVE 242 715 675 221 175 NC_003454:FN1405|5end_Formiminotetrahydrofolate_cyclodeaminase_2064287..2064517_POSITIVE 241 95 53 44 1 NC_003454:FN1247|5end_LOS_biosynthesis_enzyme_LBGB_1904344..1904574_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 304 96 51 117 76 NC_003454:FN0941|3end_Gamma-glutamyltranspeptidase_1593527..1593677_NEGATIVE 295 689 643 114 61 NC_003454:FN0200|3end_Biotin_carboxyl_carrier_protein_of_glutaconyl-COA_decarboxylase_826607..826757_POSITIVE 266 100 56 112 66 NC_003454:FN1127|3end_Hypothetical_membrane-spanning_protein_1770307..1770457_NEGATIVE 263 668 625 109 58 NC_003454:FN0269|3end_Transposase_896846..896996_POSITIVE 262 484 443 72 25 NC_003454:FN0081|3end_Ethanolamine_utilization_protein_eutL_720768..720918_POSITIVE 261 519 473 75 9 NC_003454:FN2023|3end_Hypothetical_cytosolic_protein_526172..526322_NEGATIVE 256 442 403 106 64 NC_003454:FN1501|3end_Nickel_transport_ATP-binding_protein_nikD_5928..6078_NEGATIVE 251 477 431 75 25 NC_003454:FN1107|3end_Acetyltransferase_1752367..1752517_POSITIVE 239 848 802 90 39 NC_003454:FN1611|3end_Competence_protein_120597..120747_NEGATIVE 236 964 921 90 48 NC_003454:FN1247|3end_LOS_biosynthesis_enzyme_LBGB_1903205..1903355_NEGATIVE 235 984 941 63 24 NC_003454:FN0271|3end_Enoyl-CoA_hydratase_898721..898871_POSITIVE 232 450 415 69 31 NC_003454:FN1841|3end_Dihydroxyacetone_kinase_331567..331717_POSITIVE 231 608 561 121 62 NC_003454:FN1609|3end_hypothetical_protein_118822..118972_NEGATIVE 230 88 48 86 46 NC_003454:FN1191|3end_hypothetical_protein_1854729..1854879_POSITIVE 230 494 455 122 79 NC_003454:FN1639|3end_SSU_ribosomal_protein_S3P_137504..137654_NEGATIVE 228 849 802 127 63 NC_003454:FN0650|3end_NA+/H+_antiporter_NHAC_1307717..1307867_NEGATIVE 228 798 758 111 70 NC_003454:FN0175|3end_Cell_division_inhibitor_MinC_801235..801385_POSITIVE 228 480 439 88 44 NC_003454:FN1519|3end_23S_rRNA_methyltransferase_24088..24238_NEGATIVE 226 460 411 142 96 NC_003454:FN0594|3end_(3R)-hydroxymyristoyl-[acyl_carrier_protein]_dehydratase_1249290..1249440_POSITIVE 226 685 642 96 39 NC_003454:FN0394|3end_Outer_membrane_protein_1033147..1033297_POSITIVE