Origin IGS: accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatatttttttgtacagttttgatgttagaaaactgtactttttttttattaaaaaaataatttataaaatatatataagaaaatttttttattatatttttagatgtaactatgaaattataatataaaaaattctttactaaaaaactaaaaaataaattttaaaataaattttaaatgttctataaaaaaacaaaaaaaacttatttctaaaaaataatacgattatactaaaaatatatttgacattataaattttttagatataatgaaaataagaggtaagggtatgagatttttttatgcccagataaatttaaaaaattaaataagataataaaaaatagaggtgcatatatgtaggtagtgtgaaaatgttaaggtataagccaccaatgtttcacatgaagggcatagttgccgaaagaaagttaattgcttatgattaattttcttggtcaatgtcaacaagcattgactgtcatattcttttgaatatggagagctatttatagtttttctttaattcatattgaataagactgggcaactcaatgtatatacctctattttttattaaggttgatagaaaaaatgaaggaggaggattggt Mask Tandem Repeat Region ================================================ accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacannnnnnnnnnnnnnnnnnnnnnnnnnnttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Find is-nt database================================================ Query_seq: FN1869:FN1870|FN1869:FN1870:hypothetical protein:hypothetical protein:<-<-:360930..361543 614 accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1869:FN1870|FN1869:FN1870:hypothetical protein:hypothetical protein:<-<-:360930..361543 614 accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1869:FN1870|FN1869:FN1870:hypothetical protein:hypothetical protein:<-<-:360930..361543 614 accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 101 End: 286 .................................................................................................... M N S S P Y S K E Y D S Q C L L T L T K K I N H K Q L T F F R Q L C P S C E T L V A Y T L T F S H Y L H I C T S I F Y Y ........................................................................................................................................................................................................................................................................................................................................ Protein_Len: 36 Strand: + Start: 255 End: 362 .............................................................................................................................................................................................................................................................. M Y A P L F F I I L F N F L N L S G H K K I S Y P Y L L F S L Y L K N L ............................................................................................................................................................................................................................................................ Protein_Len: 38 Strand: + Start: 358 End: 471 ..................................................................................................................................................................................................................................................................................................................................................................... M Y N V K Y I F S I I V L F F R N K F F L F F Y R T F K I Y F K I Y F L V F ............................................................................................................................................... Protein_Len: 38 Strand: + Start: 501 End: 614 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M V T S K N I I K K F S Y I Y F I N Y F F N K K K V Q F S N I K T V Q K N I Protein_Len: 41 Strand: - Start: 489 End: 611 ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ I I I E Y N C R F I Y Y F F K R I Y I K Y I I K K I F F F Y L K R V D F S Y L F M ... Protein_Len: 31 Strand: - Start: 168 End: 260 ....................................................................................................................................................................... D Y A I L K R E A V I G K M H F M P P K Y R L M K V S G V Y M .................................................................................................................................................................................................................................................................................................................................................................. accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 168 End: 197 .......................................................................................................................................................................GTTGCCGAAAGAAAGTTAATTGCTTATGAT................................................................................................................................................................................................................................................................................................................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 53 HP_score: -5.2 Tail_Score: -3.01217 Start: 135 End: 156 Full_Region: tgattaattttcttg gtcaatgt caacaa gcattgac tgtcatattcttttg ......................................................................................................................................gtcaatgtcaacaagcattgac.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: FN1869:FN1870|FN1869:FN1870:hypothetical protein:hypothetical protein:<-<-:360930..361543 614 accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctctattttttattatcttatttaattttttaaatttatctgggcataaaaaaatctcatacccttacctcttattttcattatatctaaaaaatttataatgtcaaatatatttttagtataatcgtattattttttagaaataagttttttttgtttttttatagaacatttaaaatttattttaaaatttattttttagttttttagtaaagaattttttatattataatttcatagttacatctaaaaatataataaaaaaattttcttatatatattttataaattatttttttaataaaaaaaaagtacagttttctaacatcaaaactgtacaaaaaaatatt Intra-Species Hit: Count: 1 Min: 1 Max: 603 Len: 603 Subject: NC_003454_FN1869_FN1870|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[360930,361543]|614 HSP 1 e-value: 1.0E-149 bit: 529.0 Len: 267 Query Start:1 Query End:267 Subject Strand: POSITIVE Subject Start: 1 Subject End: 267 accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctc........................................................................................................................................................................................................................................................................................................................................................... accaatcctcctccttcattttttctatcaaccttaataaaaaatagaggtatatacattgagttgcccagtcttattcaatatgaattaaagaaaaactataaatagctctccatattcaaaagaatatgacagtcaatgcttgttgacattgaccaagaaaattaatcataagcaattaactttctttcggcaactatgcccttcatgtgaaacattggtggcttataccttaacattttcacactacctacatatatgcacctc........................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 4.0E-10 bit: 67.9 Len: 34 Query Start:319 Query End:352 Subject Strand: POSITIVE Subject Start: 319 Subject End: 352 ..............................................................................................................................................................................................................................................................................................................................tctcatacccttacctcttattttcattatatct...................................................................................................................................................................................................................................................................... ..............................................................................................................................................................................................................................................................................................................................tctcatacccttacctcttattttcattatatct...................................................................................................................................................................................................................................................................... HSP 3 e-value: 2.0E-6 bit: 56.0 Len: 28 Query Start:576 Query End:603 Subject Strand: POSITIVE Subject Start: 576 Subject End: 603 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gtacagttttctaacatcaaaactgtac........... ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................gtacagttttctaacatcaaaactgtac........... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCAAUCCUCCUCCUUCAUUUUUUCUAUCAACCUUAAUAAAAAAUAGAGGUAUAUACAUUGAGUUGCCCAGUCUUAUUCAAUAUGAAUUAAAGAAAAACUAUAAAUAGCUCUCCAUAUUCAAAAGAAUAUGACAGUCAAUGCUUGUUGACAUUGACCAAGAAAAUUAAUCAUAAGCAAUUAACUUUCUUUCGGCAACUAUGCCCUUCAUGUGAAACAUUGGUGGCUUAUACCUUAACAUUUUCACACUACCUACAUAUAUGCACCUCUAUUUUUUAUUAUCUUAUUUAAUUUUUUAAAUUUAUCUGGGCAUAAAAAAAUCUCAUACCCUUACCUCUUAUUUUCAUUAUAUCUAAAAAAUUUAUAAUGUCAAAUAUAUUUUUAGUAUAAUCGUAUUAUUUUUUAGAAAUAAGUUUUUUUUGUUUUUUUAUAGAACAUUUAAAAUUUAUUUUAAAAUUUAUUUUUUAGUUUUUUAGUAAAGAAUUUUUUAUAUUAUAAUUUCAUAGUUACAUCUAAAAAUAUAAUAAAAAAAUUUUCUUAUAUAUAUUUUAUAAAUUAUUUUUUUAAUAAAAAAAAAGUACAGUUUUCUAACAUCAAAACUGUAC .(((..............................(((((((((((((((((.((((.....(((((((..(((.(((((....(((((...(((....(((....))).)))....)))))...))))).))).((((((((.....).))))))).((((((.(((((........))))).))))))..)))))))..........(((((((....((((.....)))).......)))))))...........)))).)))))))))))))))))....((((((....)))))).....)))...(((((((..........................((((((.(((((((...(((........)))..)))))))))))))..............((((((((((((...((((((.....))))))...))))))))))))........))))))).(((((((..(((((((..((((((..(((......(((......)))...((((((.(((.....))).)))))).)))..))))))..)))))))...)))))))...((((((((((........)))))))))) (-107.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUACAGUUUUGAUGUUAGAAAACUGUACUUUUUUUUUAUUAAAAAAAUAAUUUAUAAAAUAUAUAUAAGAAAAUUUUUUUAUUAUAUUUUUAGAUGUAACUAUGAAAUUAUAAUAUAAAAAAUUCUUUACUAAAAAACUAAAAAAUAAAUUUUAAAAUAAAUUUUAAAUGUUCUAUAAAAAAACAAAAAAAACUUAUUUCUAAAAAAUAAUACGAUUAUACUAAAAAUAUAUUUGACAUUAUAAAUUUUUUAGAUAUAAUGAAAAUAAGAGGUAAGGGUAUGAGAUUUUUUUAUGCCCAGAUAAAUUUAAAAAAUUAAAUAAGAUAAUAAAAAAUAGAGGUGCAUAUAUGUAGGUAGUGUGAAAAUGUUAAGGUAUAAGCCACCAAUGUUUCACAUGAAGGGCAUAGUUGCCGAAAGAAAGUUAAUUGCUUAUGAUUAAUUUUCUUGGUCAAUGUCAACAAGCAUUGACUGUCAUAUUCUUUUGAAUAUGGAGAGCUAUUUAUAGUUUUUCUUUAAUUCAUAUUGAAUAAGACUGGGCAACUCAAUGUAUAUACCUCUAUUUUUUAUUAAGGUUGAUAGAAAAAAUGAAGGAGGAGGAUUGGU .(((((((((........)))))))))(((((((((((((..............((((((.(((..(((((..(((((.(((((((.(((((..........))))).))))))).))))).)))))...........(((((......)))))..))).))))))....((((((.....(((........(((((((...............(((((((((((((((((........))))...))))))).))))))...............((((((((((...))))))))))..................))))))).((((((((((((((((((.((((((((.....((((((((.......(((....))).......)))))))).....))..(((((((..(((((((((((((......))))))))))))).((((((((......)))))))).(((.(((((...(((((..(((((((((....)))))))))....)))))....))))).)))..)))))))..)))))).))))))))))))))))))..))).))))))..)))))))))))))....... (-139.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 778 50 1 141 92 NC_003454:FN1869|5end_hypothetical_protein_360839..361069_NEGATIVE 290 252 211 196 154 NC_003454:FN1314|5end_hypothetical_protein_1968682..1968912_NEGATIVE 266 37 6 57 23 NC_003454:FN0200|5end_Biotin_carboxyl_carrier_protein_of_glutaconyl-COA_decarboxylase_826123..826353_POSITIVE 249 352 316 124 85 NC_003454:FN0754|5end_Glutamyl-tRNA(Gln)_amidotransferase_subunit_A_1414646..1414876_NEGATIVE 247 59 11 156 91 NC_003454:FN0128|5end_Spermidine/putrescine-binding_protein_756651..756881_POSITIVE 247 42 2 124 88 NC_003454:FN1829|5end_hypothetical_protein_318556..318786_NEGATIVE 232 36 2 228 198 NC_003454:FN1426|5end_Serine_protease_2095462..2095692_NEGATIVE 228 204 169 89 55 NC_003454:FN1657|5end_SSU_ribosomal_protein_S6P_153122..153352_NEGATIVE 227 51 12 200 164 NC_003454:FN0698|5end_DNA_integration/recombination/invertion_protein_1356611..1356841_POSITIVE 224 144 102 165 123 NC_003454:FN1607|5end_Cytidylate_kinase_117893..118123_NEGATIVE 221 232 202 127 95 NC_003454:FN1927|5end_DEGV_protein_427952..428182_NEGATIVE 219 222 186 190 145 NC_003454:FN0764|5end_Transporter,_LysE_family_1420327..1420557_POSITIVE 219 266 228 223 185 NC_003454:FN0118|5end_Chaperone_protein_dnaJ_750898..751128_POSITIVE 219 366 321 128 88 NC_003454:FN0079|5end_Ethanolamine_ammonia-lyase_heavy_chain_717662..717892_POSITIVE 218 30 5 170 143 NC_003454:FN0940|5end_hypothetical_protein_1593508..1593738_NEGATIVE 218 155 123 48 20 NC_003454:FN0711|5end_Phosphopantothenate--cysteine_ligase_1369569..1369799_POSITIVE 218 37 2 125 88 NC_003454:FN0084|5end_Acetaldehyde_dehydrogenase_[acetylating]_721572..721802_POSITIVE 218 159 111 111 64 NC_003454:FN1792|5end_hypothetical_protein_273264..273494_NEGATIVE 217 270 223 67 3 NC_003454:FN1495|5end_hypothetical_protein_2174410..2174640_NEGATIVE 217 270 223 91 27 NC_003454:FN1494|5end_Rod_shape-determining_protein_mreC_2174386..2174616_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 249 352 316 107 68 NC_003454:FN0755|3end_Glutamyl-tRNA(Gln)_amidotransferase_subunit_C_1414663..1414813_NEGATIVE 247 42 2 109 73 NC_003454:FN1830|3end_DNA_polymerase_III_subunits_gamma_and_tau_318571..318721_NEGATIVE 224 144 102 149 107 NC_003454:FN1608|3end_Ribosomal_protein_L11_methyltransferase_117909..118059_NEGATIVE 221 232 202 115 83 NC_003454:FN1928|3end_Transcriptional_regulator,_MerR_family_427964..428114_NEGATIVE 219 366 321 101 61 NC_003454:FN0078|3end_Ethanolamine_utilization_protein_eutA_717715..717865_POSITIVE 218 30 5 151 124 NC_003454:FN0941|3end_Gamma-glutamyltranspeptidase_1593527..1593677_NEGATIVE 218 155 123 31 3 NC_003454:FN0710|3end_hypothetical_protein_1369632..1369782_POSITIVE 217 270 223 66 2 NC_003454:FN1495|3end_hypothetical_protein_-89..61_NEGATIVE 217 286 241 95 49 NC_003454:FN1875|3end_Peptidyl-prolyl_cis-trans_isomerase_363906..364056_NEGATIVE 216 207 168 71 30 NC_003454:FN0856|3end_glycogen_branching_enzyme_1514689..1514839_NEGATIVE 214 493 454 133 89 NC_003454:FN0683|3end_hypothetical_protein_1338514..1338664_NEGATIVE 214 190 166 102 73 NC_003454:FN1847|3end_DTDP-4-dehydrorhamnose_3,5-epimerase_337208..337358_POSITIVE 214 348 301 128 82 NC_003454:FN1618|3end_hypothetical_protein_126666..126816_NEGATIVE 213 342 319 39 16 NC_003454:FN0112|3end_hypothetical_protein_746600..746750_POSITIVE 209 42 2 118 73 NC_003454:FN1034|3end_Transcriptional_regulator,_TetR_family_1688345..1688495_POSITIVE 207 348 301 94 37 NC_003454:FN1611|3end_Competence_protein_120597..120747_NEGATIVE 206 200 169 64 33 NC_003454:FN1724|3end_Potassium_uptake_protein_KtrA_222654..222804_NEGATIVE 205 229 183 90 35 NC_003454:FN0382|3end_hypothetical_protein_1014224..1014374_POSITIVE 203 38 2 88 53 NC_003454:FN1641|3end_SSU_ribosomal_protein_S19P_138552..138702_NEGATIVE 202 220 173 101 49 NC_003454:FN0643|3end_hypothetical_protein_1299434..1299584_NEGATIVE