Origin IGS: aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaataaattgattaagtaagattattgaatttatcttcattaaagtattatggtatggtagtagaaaggtaaacgtaattaagtaaatatttattataaggttgtaaggagatgatatt Mask Tandem Repeat Region ================================================ aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Find is-nt database================================================ Query_seq: FN1866:FN1867|FN1866:FN1867:Lysine 2,3-aminomutase:Zn-dependent alcohol dehydrogenase and related dehydrogenase:<-<-:358531..358649 119 aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1866:FN1867|FN1866:FN1867:Lysine 2,3-aminomutase:Zn-dependent alcohol dehydrogenase and related dehydrogenase:<-<-:358531..358649 119 aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1866:FN1867|FN1866:FN1867:Lysine 2,3-aminomutase:Zn-dependent alcohol dehydrogenase and related dehydrogenase:<-<-:358531..358649 119 aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 2 End: 118 . M S S P Y N L I I N I Y L I T F T F L L P Y H N T L M K I N S I I L L N Q F I . aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN1866:FN1867|FN1866:FN1867:Lysine 2,3-aminomutase:Zn-dependent alcohol dehydrogenase and related dehydrogenase:<-<-:358531..358649 119 aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Intra-Species Hit: Count: 1 Min: 1 Max: 119 Len: 119 Subject: NC_003454_FN1866_FN1867|Lysine 2,3-aminomutase:Zn-dependent alcohol dehydrogenase and related dehydrogenase|NEGATIVE:NEGATIVE|[358531,358649]|119 HSP 1 e-value: 1.0E-61 bit: 236.0 Len: 119 Query Start:1 Query End:119 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt aatatcatctccttacaaccttataataaatatttacttaattacgtttacctttctactaccataccataatactttaatgaagataaattcaataatcttacttaatcaatttattt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUCAUCUCCUUACAACCUUAUAAUAAAUAUUUACUUAAUUACGUUUACCUUUCUACUACCAUACCAUAAUACUUUAAUGAAGAUAAAUUCAAUAAUCUUACUUAAUCAAUUUAUUU ............................................................................(((.((((......)))).)))..................... ( -1.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAAAUUGAUUAAGUAAGAUUAUUGAAUUUAUCUUCAUUAAAGUAUUAUGGUAUGGUAGUAGAAAGGUAAACGUAAUUAAGUAAAUAUUUAUUAUAAGGUUGUAAGGAGAUGAUAUU ..(((((((.(.(((......))).).)))))))..(((((......((((..(...((((((((...((..((........))..)).))))))))...)..))))...))))).... (-12.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 722 90 41 181 132 NC_003454:FN1866|5end_Lysine_2,3-aminomutase_358440..358670_NEGATIVE 223 63 23 121 73 NC_003454:FN0084|5end_Acetaldehyde_dehydrogenase_[acetylating]_721572..721802_POSITIVE 200 50 5 117 79 NC_003454:FN1765|5end_pyruvate_kinase_259450..259680_NEGATIVE 195 78 34 130 78 NC_003454:FN1580|5end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_90919..91149_NEGATIVE 190 60 36 180 151 NC_003454:FN1095|5end_hypothetical_protein_1740232..1740462_POSITIVE 189 78 38 58 18 NC_003454:FN1451|5end_cell_division_protein_FtsZ_2132710..2132940_NEGATIVE 187 97 54 171 129 NC_003454:FN0557|5end_hypothetical_protein_1210334..1210564_NEGATIVE 185 56 11 146 101 NC_003454:FN2098|5end_MRP-family_nucleotide-binding_protein_608146..608376_NEGATIVE 183 88 55 190 150 NC_003454:FN0242|5end_Trk_system_potassium_uptake_protein_trkA_865228..865458_POSITIVE 180 87 50 174 142 NC_003454:FN1121|5end_ATP-dependent_Zn_protease_1764640..1764870_NEGATIVE 175 44 10 130 97 NC_003454:FN0660|5end_ABC_transporter_ATP-binding_protein_1317388..1317618_NEGATIVE 174 86 54 96 55 NC_003454:FN0985|5end_Phosphoribosylglycinamide_formyltransferase_1637857..1638087_NEGATIVE 173 97 53 211 173 NC_003454:FN0086|5end_hypothetical_protein_724037..724267_POSITIVE 173 78 33 102 51 NC_003454:FN2018|5end_Single-stranded-DNA-specific_exonuclease_recJ_521927..522157_NEGATIVE 170 59 11 120 59 NC_003454:FN0007|5end_glucose-inhibited_division_protein_A_645938..646168_POSITIVE 169 88 51 144 99 NC_003454:FN1791|5end_Mutator_MutT_protein_272041..272271_POSITIVE 169 77 32 192 142 NC_003454:FN1729|5end_4-amino-4-deoxychorismate_lyase_229087..229317_NEGATIVE 168 70 23 117 67 NC_003454:FN0236|5end_ABC_transporter_substrate-binding_protein_861416..861646_NEGATIVE 165 80 32 197 145 NC_003454:FN0285|5end_RNA_binding_protein_914171..914401_NEGATIVE 163 79 31 123 76 NC_003454:FN0109|5end_hypothetical_protein_744809..745039_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 179 78 47 35 6 NC_003454:FN1452|3end_Cell_division_protein_ftsA_2132733..2132883_NEGATIVE 174 86 54 108 67 NC_003454:FN0986|3end_phosphoribosylaminoimidazole_synthetase_1637845..1637995_NEGATIVE 174 84 51 36 1 NC_003454:FN0083|3end_Ethanolamine_utilization_protein_eutM_precursor_721544..721694_POSITIVE 174 69 34 139 102 NC_003454:FN1581|3end_DNA_mismatch_repair_protein_mutS_90895..91045_NEGATIVE 173 78 33 99 48 NC_003454:FN2019|3end_Ribosome-binding_factor_A_521930..522080_NEGATIVE 169 88 51 135 90 NC_003454:FN1790|3end_Cob(I)alamin_adenosyltransferase_272112..272262_POSITIVE 168 70 23 130 80 NC_003454:FN0237|3end_ABC_transporter_permease_protein_861403..861553_NEGATIVE 163 79 31 139 92 NC_003454:FN0110|3end_seryl-tRNA_synthetase_744793..744943_NEGATIVE 162 45 3 94 46 NC_003454:FN1387|3end_Metal_dependent_hydrolase_2042890..2043040_NEGATIVE 162 78 46 123 89 NC_003454:FN1033|3end_Methyltransferase_1686364..1686514_NEGATIVE 162 69 45 52 25 NC_003454:FN0973|3end_Histidinol-phosphate_aminotransferase_1625626..1625776_NEGATIVE 162 63 24 56 12 NC_003454:FN1686|3end_Spore_coat_polysaccharide_biosynthesis_protein_spsF_181027..181177_NEGATIVE 161 100 53 87 45 NC_003454:FN0716|3end_Phophatidylinositol-4-phosphate_5-kinase_1374993..1375143_POSITIVE 160 60 36 88 60 NC_003454:FN1832|3end_TonB_protein_321440..321590_NEGATIVE 158 18 1 94 75 NC_003454:FN1758|3end_Thiamin-phosphate_pyrophosphorylase_253266..253416_NEGATIVE 157 70 43 110 78 NC_003454:FN0087|3end_Ethanolamine_utilization_protein_eutN_724954..725104_POSITIVE 155 79 48 146 108 NC_003454:FN0660|3end_ABC_transporter_ATP-binding_protein_1316381..1316531_NEGATIVE 154 49 9 110 73 NC_003454:FN0490|3end_Integral_membrane_protein_1135452..1135602_NEGATIVE 154 66 25 138 94 NC_003454:FN0238|3end_hypothetical_protein_862382..862532_NEGATIVE 149 98 52 114 67 NC_003454:FN1834|3end_Biopolymer_transport_exbB_protein_322676..322826_NEGATIVE