Origin IGS: tctttcttgttcggatctttgttcttgctttaaaattgttctttcatcttcattctcattaaaagaatctgaaaaagcaaaaacattaaaaaccagaaaaatatatgttactattttttcatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatagattaatcctttctaaaaatattttcatcatttataaaattttataaaaaaataagggagttacattccagattttaagataaaaattaaatagaatgaaccgagcaaatctcaacatgtttgagctaacttgttagcgagttgattgaatttgcagcgaattcttaatttttagctattaagaaatctggctagtaatgaactattttttcttaatttaatttaaaaaggattattttatttgtcaataaaaatttttaaaaataaaattattatttataaataaagtaattctttaacagaattttttgaaatttaaggaaaatataaataaatgtaaaaaaatgggaaagtatgtaataatttaaatatacttttccttttttgttattaataaaaaaatataaaaagtacttgattttaaaagaaaaataatatataatctaacaaattttaatatataaattaatagtatgagaggaggagtatgaaaaaatagtaacatatatttttctggtttttaatgtttttgctttttcagattcttttaatgagaatgaagatgaaagaacaattttaaagcaagaacaaagatccgaacaagaaaga Mask Tandem Repeat Region ================================================ tctttcttgttcggatctttgttcttgctttaaaattgttctttcatcttcattctcattaaaagaatctgaaaaagcaaaaacattaaaaaccagaaaaatatatgttactattttttcatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Find is-nt database================================================ Query_seq: FN1818:FN1819|FN1818:FN1819:Hemolysin activator protein precursor:Export ABC transporter:<-->:307046..307658 613 tctttcttgttcggatctttgttcttgctttaaaattgttctttcatcttcattctcattaaaagaatctgaaaaagcaaaaacattaaaaaccagaaaaatatatgttactattttttcatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1818:FN1819|FN1818:FN1819:Hemolysin activator protein precursor:Export ABC transporter:<-->:307046..307658 613 tctttcttgttcggatctttgttcttgctttaaaattgttctttcatcttcattctcattaaaagaatctgaaaaagcaaaaacattaaaaaccagaaaaatatatgttactattttttcatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1818:FN1819|FN1818:FN1819:Hemolysin activator protein precursor:Export ABC transporter:<-->:307046..307658 613 tctttcttgttcggatctttgttcttgctttaaaattgttctttcatcttcattctcattaaaagaatctgaaaaagcaaaaacattaaaaaccagaaaaatatatgttactattttttcatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 1 Max: 120 Len: 120 Subject: UniRef90_Q8RGZ4 Cluster: Hemolysin activator protein; n=2; Fusobacterium nucleatum subsp. nucleatum|Rep: Hemolysin activator protein - Fusobacterium nucleatum subsp. nucleatum HSP 1 e-value: 3.0E-13 bit: 77.4 Len: 120 Query Start:1 Query End:120 Subject Strand: null Subject Start: 2 Subject End: 41 E K I V T Y I F L V F N V F A F S D S F N E N E D E R T I L K Q E Q R S E Q E R ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. K K I G T Y I F L V F N V F A F S D S F N E N E D E R T I L K Q E Q R S E Q E R ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: + Start: 35 End: 163 .................................. M V L S S S F S L K E S E K A K T L K T R K I Y V T I F S Y S S S H T I N L Y I K I C .................................................................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 46 Strand: + Start: 105 End: 242 ........................................................................................................ M L L F F H T P P L I L L I Y I L K F V R L Y I I F L L K S S T F Y I F L L I T K K E K Y I ................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 56 Strand: + Start: 167 End: 334 ...................................................................................................................................................................... M I Y Y F S F K I K Y F L Y F F I N N K K G K V Y L N Y Y I L S H F F T F I Y I F L K F Q K I L L K N Y F I Y K ....................................................................................................................................................................................................................................................................................... Protein_Len: 38 Strand: - Start: 1 End: 114 R E Q E S R Q E Q K L I T R E D E N E N F S D S F A F V N F V L F I Y T V M ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 88 Start: 361 End: 390 ........................................................................................................................................................................................................................................................................................................................................................................ATTGACAAATAAAATAATCCTTTTTAAATT............................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 167 End: 196 ......................................................................................................................................................................CTTGATTTTAAAAGAAAAATAATATATAAT................................................................................................................................................................................................................................................................................................................................................................................................................................. PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 268 End: 297 ...........................................................................................................................................................................................................................................................................TTTTACATTTATTTATATTTTCCTTAAATT............................................................................................................................................................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 340 End: 369 ...................................................................................................................................................................................................................................................................................................................................................TTTGTCAATAAAAATTTTTAAAAATAAAAT.................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 68 HP_score: -2.6 Tail_Score: -5.66229 Start: 230 End: 263 Full_Region: taaatgtaaaaaaat gggaaagtatgt aataatttaa atatacttttcc ttttttgttattaat .....................................................................................................................................................................................................................................gggaaagtatgtaataatttaaatatacttttcc.............................................................................................................................................................................................................................................................................................................................................................. TransTerm Strand: + Conf: 47 HP_score: -2.9 Tail_Score: -3.93963 Start: 312 End: 326 Full_Region: TTCAAAAAATTCTGT TAAAG AATTA CTTTA TTTATAAATAATAAT .......................................................................................................................................................................................................................................................................................................................TAAAGAATTACTTTA............................................................................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 60 HP_score: -5.2 Tail_Score: -3.91488 Start: 319 End: 377 Full_Region: ttaatttaaaaagga ttattttatttgtcaataaaaatttt taaaaat aaaattattatttataaataaagtaa ttctttaacagaatt ..............................................................................................................................................................................................................................................................................................................................ttattttatttgtcaataaaaatttttaaaaataaaattattatttataaataaagtaa............................................................................................................................................................................................................................................ TransTerm Strand: + Conf: 37 HP_score: -2.5 Tail_Score: -2.77538 Start: 327 End: 353 Full_Region: TAAAGAATTACTTTA TTTATAAATAA TAATT TTATTTTTAAA AATTTTTATTGACAA ......................................................................................................................................................................................................................................................................................................................................TTTATAAATAATAATTTTATTTTTAAA.................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 40 HP_score: -2.6 Tail_Score: -3.06831 Start: 425 End: 448 Full_Region: CATTACTAGCCAGAT TTCTTAAT AGCTAAAA ATTAAGAA TTCGCTGCAAATTCA ........................................................................................................................................................................................................................................................................................................................................................................................................................................TTCTTAATAGCTAAAAATTAAGAA..................................................................................................................................................................... TransTerm Strand: + Conf: 45 HP_score: -4.3 Tail_Score: -2.87586 Start: 485 End: 499 Full_Region: TCGCTAACAAGTTAG CTCAA ACATG TTGAG ATTTGCTCGGTTCAT ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................CTCAAACATGTTGAG.................................................................................................................. Find igs database================================================ Query_seq: FN1818:FN1819|FN1818:FN1819:Hemolysin activator protein precursor:Export ABC transporter:<-->:307046..307658 613 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatactcctcctctcatactattaatttatatattaaaatttgttagattatatattatttttcttttaaaatcaagtactttttatatttttttattaataacaaaaaaggaaaagtatatttaaattattacatactttcccatttttttacatttatttatattttccttaaatttcaaaaaattctgttaaagaattactttatttataaataataattttatttttaaaaatttttattgacaaataaaataatcctttttaaattaaattaagaaaaaatagttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt Intra-Species Hit: Count: 9 Min: 219 Max: 613 Len: 395 Subject: NC_003454_FN1818_FN1819|Hemolysin activator protein precursor:Export ABC transporter|NEGATIVE:POSITIVE|[307046,307658]|613 HSP 1 e-value: 1.0E-101 bit: 371.0 Len: 208 Query Start:406 Query End:613 Subject Strand: POSITIVE Subject Start: 406 Subject End: 613 .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttannnnnnnataaaattttataaatgatgaaaatatttttagaaaggattaatctatt .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactcccttatttttttataaaattttataaatgatgaaaatatttttagaaaggattaatctatt HSP 2 e-value: 3.0E-17 bit: 91.7 Len: 46 Query Start:219 Query End:264 Subject Strand: POSITIVE Subject Start: 219 Subject End: 264 ..........................................................................................................................................................................................................................ataacaaaaaaggaaaagtatatttaaattattacatactttccca............................................................................................................................................................................................................................................................................................................................................................. ..........................................................................................................................................................................................................................ataacaaaaaaggaaaagtatatttaaattattacatactttccca............................................................................................................................................................................................................................................................................................................................................................. Subject: NC_003454_FN0391_FN0392|Hydrolase (HAD superfamily):Oxygen-independent coproporphyrinogen III oxidase|POSITIVE:POSITIVE|[1029557,1029763]|207 HSP 1 e-value: 1.0E-56 bit: 222.0 Len: 152 Query Start:406 Query End:557 Subject Strand: POSITIVE Subject Start: 5 Subject End: 156 .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactccctta........................................................ .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcgttactagccagatttcttaacgaataaaaattaagaatttgctgcaaattcaaccaactcgctaacaagttagctcaaacatgttgagatttgctcggctcattctatttaatttttatcctaaaatctggaatgtaactctctta........................................................ Subject: NC_003454_FN1835_FN1836|hypothetical protein:Tetratricopeptide repeat family protein|NEGATIVE:NEGATIVE|[323771,323993]|223 HSP 1 e-value: 8.0E-33 bit: 143.0 Len: 147 Query Start:406 Query End:552 Subject Strand: NEGATIVE Subject Start: 62 Subject End: 209 .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagct-caaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactc............................................................. .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcgttactagccagatttcttaacggataaaaattaagaattcgctgcaaatttgacaaacttgctgacaagtcagcttcaaacaagtcaagatttgctcggctcattctatttaatttttatcctaaaatctggaatataactc............................................................. Subject: NC_003454_FN1808_FN1809|hypothetical protein:Iron/zinc/copper-binding protein|NEGATIVE:NEGATIVE|[289996,290269]|274 HSP 1 e-value: 1.0E-19 bit: 99.6 Len: 54 Query Start:498 Query End:551 Subject Strand: NEGATIVE Subject Start: 89 Subject End: 142 .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaact.............................................................. .................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................agatttgctcggttcattttatttaatttttatcttaaaatctggaatgtaact.............................................................. HSP 2 e-value: 6.0E-12 bit: 73.8 Len: 53 Query Start:409 Query End:461 Subject Strand: NEGATIVE Subject Start: 215 Subject End: 267 ........................................................................................................................................................................................................................................................................................................................................................................................................................tcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaatt........................................................................................................................................................ ........................................................................................................................................................................................................................................................................................................................................................................................................................tcattactagtcagatttcttaacagataaaaattaagaattcactgcaaatt........................................................................................................................................................ Subject: NC_003454_FN2095_FN2096|General secretion pathway protein E:hypothetical protein|NEGATIVE:NEGATIVE|[606171,606356]|186 HSP 1 e-value: 1.0E-16 bit: 89.7 Len: 57 Query Start:407 Query End:463 Subject Strand: NEGATIVE Subject Start: 125 Subject End: 181 ......................................................................................................................................................................................................................................................................................................................................................................................................................gttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattca...................................................................................................................................................... ......................................................................................................................................................................................................................................................................................................................................................................................................................gttcatcactagccagatttcttaacagataaaaattaagaattcgctgcaaattca...................................................................................................................................................... HSP 2 e-value: 2.0E-12 bit: 75.8 Len: 54 Query Start:496 Query End:549 Subject Strand: NEGATIVE Subject Start: 46 Subject End: 99 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaa................................................................ ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagatttgctcggctcattctatttaatttttattctagaatctggaatgtaa................................................................ Subject: NC_003454_FN0713_FN0714|Chromate transport protein:NADH oxidase|POSITIVE:POSITIVE|[1372004,1372201]|198 HSP 1 e-value: 7.0E-15 bit: 83.8 Len: 58 Query Start:500 Query End:557 Subject Strand: NEGATIVE Subject Start: 20 Subject End: 77 ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atttgctcggttcattctatttaatttttatcttaaaatctggaatgtaactccctta........................................................ ...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................atttgcttggctcattctatttaatttttatcctaaaatctggaatgtaactctctta........................................................ HSP 2 e-value: 1.0E-13 bit: 79.8 Len: 56 Query Start:408 Query End:463 Subject Strand: NEGATIVE Subject Start: 107 Subject End: 162 .......................................................................................................................................................................................................................................................................................................................................................................................................................ttcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattca...................................................................................................................................................... .......................................................................................................................................................................................................................................................................................................................................................................................................................ttcattactagccagatttcttaacagataaaaattaaaaattcactgcaaattca...................................................................................................................................................... Subject: NC_003454_FN0483_FN0484|Uracil phosphoribosyltransferase:Lipase|POSITIVE:POSITIVE|[1129 362,1129609]|248 HSP 1 e-value: 7.0E-15 bit: 83.8 Len: 54 Query Start:496 Query End:549 Subject Strand: POSITIVE Subject Start: 96 Subject End: 149 ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagatttgctcggttcattctatttaatttttatcttaaaatctggaatgtaa................................................................ ...............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tgagatttgctcggctcattctatttaatttttatcctaaaatttggaatgtaa................................................................ HSP 2 e-value: 2.0E-12 bit: 75.8 Len: 54 Query Start:409 Query End:462 Subject Strand: POSITIVE Subject Start: 16 Subject End: 69 ........................................................................................................................................................................................................................................................................................................................................................................................................................tcattactagccagatttcttaatagctaaaaattaagaattcgctgcaaattc....................................................................................................................................................... ........................................................................................................................................................................................................................................................................................................................................................................................................................tcattactagccagatttcctaacggataaaaattaagaattcgctgcaaattc....................................................................................................................................................... Subject: NC_003454_FN0084_FN0085|Acetaldehyde dehydrogenase [acetylating]:Ethanolamine utilization cobalamin adenosyltransferase|POSITIVE:POSITIVE|[723155, 723409]|255 HSP 1 e-value: 1.0E-13 bit: 79.8 Len: 52 Query Start:506 Query End:557 Subject Strand: POSITIVE Subject Start: 155 Subject End: 206 .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcggttcattctatttaatttttatcttaaaatctggaatgtaactccctta........................................................ .........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................tcggctcattctatttaatttttatcctaaaatctggaatgtaactcactta........................................................ HSP 2 e-value: 1.0E-7 bit: 60.0 Len: 50 Query Start:406 Query End:455 Subject Strand: POSITIVE Subject Start: 12 Subject End: 61 .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcattactagccagatttcttaatagctaaaaattaagaattcgctg.............................................................................................................................................................. .....................................................................................................................................................................................................................................................................................................................................................................................................................agttcgttactagccaaatttcttaacggataaaaattaagaattcgctg.............................................................................................................................................................. Subject: NC_003454_FN1651_FN1652|Oligopeptide transport system permease protein oppB:Oligopeptide-binding protein oppA|NEGATIVE:NEGATIVE|[145890,146130]|241 HSP 1 e-value: 2.0E-12 bit: 75.8 Len: 120 Query Start:421 Query End:540 Subject Strand: POSITIVE Subject Start: 56 Subject End: 168 ....................................................................................................................................................................................................................................................................................................................................................................................................................................agatttcttaatagctaaaaattaagaattcgctgcaaattcaatcaactcgctaacaagttagctcaaacatgttgagatttgctcggttcattctatttaatttttatcttaaaatct......................................................................... ....................................................................................................................................................................................................................................................................................................................................................................................................................................agatttcttaacggataaaaattaagaattcgctgtaaattctaccaactcgcta-------tgctcagacacgtcgagatttactcggctcattctatttaattttcatcctaaaatct......................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAACAAAAAAGGAAAAGUAUAUUUAAAUUAUUACAUACUUUCCCAUUUUUUUACAUUUAUUUAUAUUUUCCUUAAAUUUCAAAAAAUUCUGUUAAAGAAUUACUUUAUUUAUAAAUAAUAAUUUUAUUUUUAAAAAUUUUUAUUGACAAAUAAAAUAAUCCUUUUUAAAUUAAAUUAAGAAAAAAUAGUUCAUUACUAGCCAGAUUUCUUAAUAGCUAAAAAUUAAGAAUUCGCUGCAAAUUCAAUCAACUCGCUAACAAGUUAGCUCAAACAUGUUGAGAUUUGCUCGGUUCAUUCUAUUUAAUUUUUAUCUUAAAAUCUGGAAUGUAACUCCCUUAUUUUUUUAUAAAAUUUUAUAAAUGAUGAAAAUAUUUUUAGAAAGGAUUAAUCUAUU .........((((((((.((((..(((((..........................)))))..))))))))))))...........((((((.....))))))..(((((((...(((((.((((((.......)))))).)))))...))))))).((((((((((((((.....(((.((((((((((....((((...((((((((......((.((((((((((((((..((((((((((...(((((..(((((....)))))........))))))))))))..)))..)))))...))))))))).))..))))))))...)))).....)))))))))).)))..((((((.....)))))).....))))))))))))))....... (-73.07) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAGAUUAAUCCUUUCUAAAAAUAUUUUCAUCAUUUAUAAAAUUUUAUAAAAAAAUAAGGGAGUUACAUUCCAGAUUUUAAGAUAAAAAUUAAAUAGAAUGAACCGAGCAAAUCUCAACAUGUUUGAGCUAACUUGUUAGCGAGUUGAUUGAAUUUGCAGCGAAUUCUUAAUUUUUAGCUAUUAAGAAAUCUGGCUAGUAAUGAACUAUUUUUUCUUAAUUUAAUUUAAAAAGGAUUAUUUUAUUUGUCAAUAAAAAUUUUUAAAAAUAAAAUUAUUAUUUAUAAAUAAAGUAAUUCUUUAACAGAAUUUUUUGAAAUUUAAGGAAAAUAUAAAUAAAUGUAAAAAAAUGGGAAAGUAUGUAAUAAUUUAAAUAUACUUUUCCUUUUUUGUUAU ..((((.........))))...((((((((...((((..((((((((...((((((((.(...(((((...(((((((((.((.(((((((((...(((((....((.((((((.((((((.......(((((....)))))....)).))))))))))..)).)))))))))))))).)).....)))))))))...)))))...)))))))))...............(((((((((((((((((((.(((((.((((((.......)))))).))))).)))))))))))))))))))...))))))))...)))).....))))))))...........((((((.((((((((((((..........))))))))))))))))))..... (-84.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 668 300 251 95 45 NC_003454:FN1460|5end_Hypothetical_cytosolic_protein_2141429..2141659_POSITIVE 640 240 191 178 129 NC_003454:FN0714|5end_NADH_oxidase_1372062..1372292_POSITIVE 614 299 251 130 81 NC_003454:FN1604|5end_hypothetical_protein_113822..114052_NEGATIVE 529 44 1 231 188 NC_003454:FN0131|5end_Hemolysin_activator_protein_precursor_759547..759777_POSITIVE 497 290 241 227 185 NC_003454:FN1651|5end_Oligopeptide_transport_system_permease_protein_oppB_145799..146029_NEGATIVE 414 240 191 223 174 NC_003454:FN0798|5end_Fructose-1,6-bisphosphatase_1455835..1456065_POSITIVE 397 340 294 225 182 NC_003454:FN1615|5end_Integral_membrane_protein_125125..125355_POSITIVE 380 210 162 130 84 NC_003454:FN1440|5end_1-phosphofructokinase_2107238..2107468_POSITIVE 346 229 181 220 172 NC_003454:FN1603|5end_2',3'-cyclic_nucleotide_3'-phosphodiesterase_113661..113891_NEGATIVE 340 345 304 194 153 NC_003454:FN0367|5end_Integral_membrane_protein_996901..997131_NEGATIVE 337 300 251 129 87 NC_003454:FN1507|5end_riboflavin_synthase_subunit_alpha_12073..12303_POSITIVE 299 230 181 161 112 NC_003454:FN0081|5end_Ethanolamine_utilization_protein_eutL_720035..720265_POSITIVE 281 365 325 135 92 NC_003454:FN0832|5end_hypothetical_protein_1494305..1494535_POSITIVE 244 344 321 156 133 NC_003454:FN1898|5end_Sugar_transport_ATP-binding_protein_386569..386799_NEGATIVE 240 75 31 104 66 NC_003454:FN0753|5end_aspartyl/glutamyl-tRNA_amidotransferase_subunit_B_1413169..1413399_NEGATIVE 228 305 267 230 191 NC_003454:FN1209|5end_Hypothetical_RNA_binding_protein_1868914..1869144_NEGATIVE 222 300 251 213 154 NC_003454:FN1482|5end_Guanosine-3',5'-bis(Diphosphate)_3'-pyrophosphohydrolase_2165992..2166222_NEGATIVE 218 229 185 203 160 NC_003454:FN0781|5end_Integral_membrane_protein_1438281..1438511_NEGATIVE 214 263 229 207 174 NC_003454:FN1167|5end_beta-methylgalactoside_transporter_inner_membrane_component_1825538..1825768_POSITIVE 214 249 201 207 158 NC_003454:FN0392|5end_Oxygen-independent_coproporphyrinogen_III_oxidase_1029624..1029854_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 619 50 1 82 33 NC_003454:FN0130|3end_ABC_transporter_ATP-binding_protein_759472..759622_POSITIVE 601 340 291 72 23 NC_003454:FN0713|3end_Chromate_transport_protein_1371943..1372093_POSITIVE 380 210 162 57 11 NC_003454:FN1439|3end_Transcriptional_regulator,_DeoR_family_2107245..2107395_POSITIVE 340 345 304 57 16 NC_003454:FN0368|3end_adenylosuccinate_lyase_997038..997188_NEGATIVE 334 320 271 56 7 NC_003454:FN1506|3end_5-amino-6-(5-phosphoribosylamino)uracil_reductase_12060..12210_POSITIVE 324 330 289 42 1 NC_003454:FN0796|3end_pyruvate_phosphate_dikinase_1455643..1455793_POSITIVE 299 230 181 54 5 NC_003454:FN0080|3end_ethanolamine_ammonia-lyase_small_subunit_720008..720158_POSITIVE 298 297 251 48 1 NC_003454:FN2094|3end_General_secretion_pathway_protein_F_603799..603949_NEGATIVE 258 345 306 41 1 NC_003454:FN1652|3end_Oligopeptide-binding_protein_oppA_146041..146191_NEGATIVE 250 230 182 54 1 NC_003454:FN1427|3end_Phenazine_biosynthesis_protein_phzF_2096763..2096913_POSITIVE 240 75 31 90 52 NC_003454:FN0754|3end_Glutamyl-tRNA(Gln)_amidotransferase_subunit_A_1413183..1413333_NEGATIVE 238 93 55 111 72 NC_003454:FN1901|3end_Transcription_regulator,_CRP_family_389364..389514_NEGATIVE 209 304 274 73 38 NC_003454:FN1023|3end_5-Nitroimidazole_antibiotic_resistance_protein_1677560..1677710_NEGATIVE 207 56 11 72 17 NC_003454:FN0363|3end_ATP_synthase_C_chain,_sodium_ion_specific_993434..993584_NEGATIVE 205 219 177 81 40 NC_003454:FN1010|3end_Hypothetical_cytosolic_protein_1664312..1664462_NEGATIVE 201 240 193 134 70 NC_003454:FN0201|3end_Glutaconyl-COA_decarboxylase_beta_subunit_827749..827899_POSITIVE 190 338 295 77 33 NC_003454:FN1836|3end_Tetratricopeptide_repeat_family_protein_323904..324054_NEGATIVE 190 288 252 37 5 NC_003454:FN1533|3end_Electron_transfer_flavoprotein_alpha-subunit_45350..45500_NEGATIVE 188 318 274 89 37 NC_003454:FN0445|3end_hypothetical_protein_1085774..1085924_POSITIVE 188 344 307 121 89 NC_003454:FN1545|3end_Ferredoxin_like_protein_59497..59647_POSITIVE