Origin IGS: tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 cgaaattaattggctacttgccagccattagtatttcaagagctccaaaatgctctttcaatattaatggacgtcacagtagcctatctaaataaatataactcattaattttttaaatagtaaagaataatagaaactaaggaagaaaggggtaataaa Mask Tandem Repeat Region ================================================ tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Find is-nt database================================================ Query_seq: FN1740:FN1741|FN1740:FN1741:ATP synthase subunit K:ATP synthase subunit I:<-<-:238491..238650 160 tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1740:FN1741|FN1740:FN1741:ATP synthase subunit K:ATP synthase subunit I:<-<-:238491..238650 160 tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1740:FN1741|FN1740:FN1741:ATP synthase subunit K:ATP synthase subunit I:<-<-:238491..238650 160 tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 47 End: 151 .............................................. F I L S N Y K N L Y A V T V D M L I S L A N Q L E Q F V L P Q C T A M ......... tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN1740:FN1741|FN1740:FN1741:ATP synthase subunit K:ATP synthase subunit I:<-<-:238491..238650 160 tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Intra-Species Hit: Count: 1 Min: 1 Max: 160 Len: 160 Subject: NC_003454_FN1740_FN1741|ATP synthase subunit K:ATP synthase subunit I|NEGATIVE:NEGATIVE|[238491,238650]|160 HSP 1 e-value: 6.0E-86 bit: 317.0 Len: 160 Query Start:1 Query End:160 Subject Strand: POSITIVE Subject Start: 1 Subject End: 160 tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg tttattacccctttcttccttagtttctattattctttactatttaaaaaattaatgagttatatttatttagataggctactgtgacgtccattaatattgaaagagcattttggagctcttgaaatactaatggctggcaagtagccaattaatttcg Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAUUACCCCUUUCUUCCUUAGUUUCUAUUAUUCUUUACUAUUUAAAAAAUUAAUGAGUUAUAUUUAUUUAGAUAGGCUACUGUGACGUCCAUUAAUAUUGAAAGAGCAUUUUGGAGCUCUUGAAAUACUAAUGGCUGGCAAGUAGCCAAUUAAUUUCG .........................((((.........(((..((((....))))..)))..........))))..(((((((((.(.(.((((((.((((..((((((........))))))..)))).)))))))).)).)))))))........... (-33.91) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGAAAUUAAUUGGCUACUUGCCAGCCAUUAGUAUUUCAAGAGCUCCAAAAUGCUCUUUCAAUAUUAAUGGACGUCACAGUAGCCUAUCUAAAUAAAUAUAACUCAUUAAUUUUUUAAAUAGUAAAGAAUAAUAGAAACUAAGGAAGAAAGGGGUAAUAAA ...........(((((((((.(..(((((((((((..((((((........))))))..)))))))))))..).)).)))))))............(((.((((.....((((((...((((..............))))...)))))).)))).))).. (-38.54) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 869 120 71 211 162 NC_003454:FN1740|5end_ATP_synthase_subunit_K_238400..238630_NEGATIVE 751 146 101 231 186 NC_003454:FN1142|5end_Oxygen-independent_coproporphyrinogen_III_oxidase_1791529..1791759_POSITIVE 688 148 101 213 166 NC_003454:FN1439|5end_Transcriptional_regulator,_DeoR_family_2106428..2106658_POSITIVE 688 148 101 109 62 NC_003454:FN1438|5end_hypothetical_protein_2106324..2106554_POSITIVE 578 146 101 221 178 NC_003454:FN1995|5end_hypothetical_protein_498245..498475_POSITIVE 528 129 82 193 146 NC_003454:FN1351|5end_15_kDa_lipoprotein_precursor_2003372..2003602_NEGATIVE 525 137 91 231 185 NC_003454:FN1581|5end_DNA_mismatch_repair_protein_mutS_93231..93461_NEGATIVE 484 116 71 231 185 NC_003454:FN1731|5end_Anthranilate_synthase_component_II_231037..231267_NEGATIVE 450 138 91 230 182 NC_003454:FN0054|5end_tyrosyl-tRNA_synthetase_690836..691066_POSITIVE 370 118 71 222 174 NC_003454:FN0186|5end_Cytochrome_c-type_biogenesis_protein_ccdA_810853..811083_POSITIVE 349 127 81 226 181 NC_003454:FN1328|5end_Exodeoxyribonuclease_VII_small_subunit_1983129..1983359_NEGATIVE 287 145 105 190 148 NC_003454:FN1799|5end_Spermidine/putrescine_transport_system_permease_protein_potC_280776..281006_POSITIVE 262 129 81 181 132 NC_003454:FN0053|5end_Branched-chain_amino_acid_transport_system_carrier_protein_689340..689570_POSITIVE 261 118 72 215 169 NC_003454:FN0220|5end_Autolysin_sensor_kinase_844934..845164_NEGATIVE 245 149 112 114 72 NC_003454:FN0620|5end_hypothetical_protein_1279563..1279793_POSITIVE 240 150 102 90 51 NC_003454:FN1733|5end_ATP_synthase_subunit_D_232871..233101_NEGATIVE 235 141 107 171 138 NC_003454:FN1722|5end_Glucose_inhibited_division_protein_B_220742..220972_NEGATIVE 230 139 91 152 108 NC_003454:FN1526|5end_Fusobacterium_outer_membrane_protein_family_38183..38413_NEGATIVE 227 150 105 199 148 NC_003454:FN0344|5end_Methyltransferase_977761..977991_NEGATIVE 227 143 118 33 6 NC_003454:FN1658|5end_prolyl-tRNA_synthetase_154891..155121_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 584 148 101 79 32 NC_003454:FN0828|3end_ABC_transporter_permease_protein_1489505..1489655_POSITIVE 528 129 82 59 12 NC_003454:FN1352|3end_ABC_transporter_ATP-binding_protein_2003506..2003656_NEGATIVE 436 138 91 56 8 NC_003454:FN1259|3end_Uracil-DNA_glycosylase_1915756..1915906_POSITIVE 307 119 72 77 37 NC_003454:FN1200|3end_hypothetical_protein_1860227..1860377_NEGATIVE 262 129 81 52 3 NC_003454:FN0052|3end_Arsenate_reductase_689291..689441_POSITIVE 251 151 114 39 2 NC_003454:FN1329|3end_Methyltransferase_1983342..1983492_NEGATIVE 240 150 102 79 40 NC_003454:FN1734|3end_V-type_ATP_synthase_subunit_B_232882..233032_NEGATIVE 238 139 107 146 121 NC_003454:FN1253|3end_hypothetical_protein_1910229..1910379_NEGATIVE 217 130 83 96 50 NC_003454:FN0013|3end_Transporter_652639..652789_POSITIVE 212 41 12 103 75 NC_003454:FN1645|3end_LSU_ribosomal_protein_L3P_140666..140816_NEGATIVE 208 44 1 130 87 NC_003454:FN1993|3end_SUA5_protein_497650..497800_POSITIVE 206 38 1 130 90 NC_003454:FN1158|3end_hypothetical_protein_1814814..1814964_POSITIVE 204 46 1 93 37 NC_003454:FN1539|3end_Iron-sulfur_cluster-binding_protein_53092..53242_POSITIVE 202 45 10 109 73 NC_003454:FN1488|3end_Methenyltetrahydrofolate_cyclohydrolase_2170035..2170185_NEGATIVE 199 129 81 56 8 NC_003454:FN1077|3end_hypothetical_protein_1723754..1723904_NEGATIVE 199 126 81 73 20 NC_003454:FN2034|3end_Protein_yicC_537917..538067_NEGATIVE 198 92 57 91 44 NC_003454:FN0388|3end_Tetracenomycin_polyketide_synthesis_O-methyltransferase_tcmP_1026600..1026750_NEGATIVE 196 41 2 108 63 NC_003454:FN0678|3end_Ser/Thr_protein_kinase_1334651..1334801_POSITIVE 196 62 36 101 72 NC_003454:FN0538|3end_Protein_translocase_subunit_secG_1192151..1192301_POSITIVE 194 150 119 128 96 NC_003454:FN1353|3end_ABC_transporter_permease_protein_2004184..2004334_NEGATIVE