Origin IGS: aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgataaaaaatttctatcaatatcatttttaaataaaaaatattgagacaatcaaacttccttattaaaattagattgctaaaaataaccataaaagaaagtgacttcattacctctatctaattttagcaaaactatctaaaatattcaagataacaatatattctttccagaagaagactattatcaaattataaaaaaatgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gcaagaacaataattaaattaaaaaaactttaaaaatttttattaggtaatcatttttttataatttgataatagtcttcttctggaaagaatatattgttatcttgaatattttagatagttttgctaaaattagatagaggtaatgaagtcactttcttttatggttatttttagcaatctaattttaataaggaagtttgattgtctcaatattttttatttaaaaatgatattgatagaaattttttatcaacatcatttattatttaactctaattttttataattatgactttaaattaaacttaaaatacttgagagcttttagaaaatataatattttgtcatcaagtttactgatttttagggggagttt Mask Tandem Repeat Region ================================================ aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgataaaaaatttctatcaatatcatttttaaataaaaaatattgagacaatcaaacttccttattaaaattagattgctaaaaataaccataaaagaaagtgacttcattacctctatctaattttagcaaaactatctaaaatattcaagataacaatatattctttccagaagaagactattatcaaattataaaaaaatgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Find is-nt database================================================ Query_seq: FN1672:FN1673|FN1672:FN1673:Membrane-bound O-acyltransferase:hypothetical protein:<-<-:171384..171762 379 aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgataaaaaatttctatcaatatcatttttaaataaaaaatattgagacaatcaaacttccttattaaaattagattgctaaaaataaccataaaagaaagtgacttcattacctctatctaattttagcaaaactatctaaaatattcaagataacaatatattctttccagaagaagactattatcaaattataaaaaaatgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1672:FN1673|FN1672:FN1673:Membrane-bound O-acyltransferase:hypothetical protein:<-<-:171384..171762 379 aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgataaaaaatttctatcaatatcatttttaaataaaaaatattgagacaatcaaacttccttattaaaattagattgctaaaaataaccataaaagaaagtgacttcattacctctatctaattttagcaaaactatctaaaatattcaagataacaatatattctttccagaagaagactattatcaaattataaaaaaatgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1672:FN1673|FN1672:FN1673:Membrane-bound O-acyltransferase:hypothetical protein:<-<-:171384..171762 379 aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgataaaaaatttctatcaatatcatttttaaataaaaaatattgagacaatcaaacttccttattaaaattagattgctaaaaataaccataaaagaaagtgacttcattacctctatctaattttagcaaaactatctaaaatattcaagataacaatatattctttccagaagaagactattatcaaattataaaaaaatgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 129 Max: 326 Len: 198 Subject: UniRef90_Q8RFA4 Cluster: Hypothetical cytosolic protein; n=1; Fusobacterium nucleatum subsp. nucleatum|Rep: Hypothetical cytosolic protein - Fusobacterium nucleatum subsp. nucleatum HSP 1 e-value: 3.0E-7 bit: 56.2 Len: 198 Query Start:129 Query End:326 Subject Strand: null Subject Start: 4 Subject End: 66 ................................................................................................................................ K K F L S I S F L N K K Y * D N Q T S L L K L D C * K * P * K K V T S L P L S N F S K T I * N I Q D N N I F F P E E D Y Y Q I I K K ..................................................... ................................................................................................................................ K K F L S A S F F N K K K L R Q Q N F P V K I K L L K I T I K E V I L L S L S N - - - S I L N I Q D N N T F F S E K E Y Y Q I I K E ..................................................... aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Predict ORF larger than 30AA ================================================ Protein_Len: 50 Strand: - Start: 94 End: 243 ............................................................................................. L F N S N F L L H H Q Y F I E I L I M K L Y F I N L C D F K R I L I L N S F I V M F S L S K M V E M ........................................................................................................................................ Protein_Len: 42 Strand: - Start: 3 End: 128 .. S G R F I L L S S S L I N Y K R F A R L Y K L N L K F D Y N Y F I L T L Y Y I I N M ........................................................................................................................................................................................................................................................... aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 86 Start: 166 End: 195 .....................................................................................................................................................................ATTGAGACAATCAAACTTCCTTATTAAAAT........................................................................................................................................................................................ PromScan Matrix: RpoD-17 Strand: - Score: 86 Start: 247 End: 276 ......................................................................................................................................................................................................................................................CTTGAATATTTTAGATAGTTTTGCTAAAAT....................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 90 End: 119 .........................................................................................ATTTATTATTTAACTCTAATTTTTTATAAT.................................................................................................................................................................................................................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 315 End: 344 ..........................................................................................................................................................................................................................................................................................................................ATTTTTATTAGGTAATCATTTTTTTATAAT................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 82 HP_score: -7.8 Tail_Score: -4.65895 Start: 119 End: 149 Full_Region: GAGTTAAATAATAAA TGATGTTGATAAAA AAT TTCTATCAATATCA TTTTTAAATAAAAAA ......................................................................................................................TGATGTTGATAAAAAATTTCTATCAATATCA...................................................................................................................................................................................................................................... TransTerm Strand: - Conf: 82 HP_score: -7.8 Tail_Score: -4.58585 Start: 119 End: 149 Full_Region: ttttttatttaaaaa tgatattgatagaa att ttttatcaacatca tttattatttaactc ......................................................................................................................tgatattgatagaaattttttatcaacatca...................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: FN1672:FN1673|FN1672:FN1673:Membrane-bound O-acyltransferase:hypothetical protein:<-<-:171384..171762 379 aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgatnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Intra-Species Hit: Count: 1 Min: 1 Max: 379 Len: 379 Subject: NC_003454_FN1672_FN1673|Membrane-bound O-acyltransferase:hypothetical protein|NEGATIVE:NEGATIVE|[171384,171762]|379 HSP 1 e-value: 2.0E-66 bit: 254.0 Len: 128 Query Start:1 Query End:128 Subject Strand: POSITIVE Subject Start: 1 Subject End: 128 aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgat........................................................................................................................................................................................................................................................... aaactccccctaaaaatcagtaaacttgatgacaaaatattatattttctaaaagctctcaagtattttaagtttaatttaaagtcataattataaaaaattagagttaaataataaatgatgttgat........................................................................................................................................................................................................................................................... HSP 2 e-value: 4.0E-9 bit: 63.9 Len: 53 Query Start:327 Query End:379 Subject Strand: POSITIVE Subject Start: 327 Subject End: 379 ......................................................................................................................................................................................................................................................................................................................................tgattacctaataaaaatttttaaagnnnnnnnaatttaattattgttcttgc ......................................................................................................................................................................................................................................................................................................................................tgattacctaataaaaatttttaaagtttttttaatttaattattgttcttgc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAACUCCCCCUAAAAAUCAGUAAACUUGAUGACAAAAUAUUAUAUUUUCUAAAAGCUCUCAAGUAUUUUAAGUUUAAUUUAAAGUCAUAAUUAUAAAAAAUUAGAGUUAAAUAAUAAAUGAUGUUGAUAAAAAAUUUCUAUCAAUAUCAUUUUUAAAUAAAAAAUAUUGAGACAAUCAAACUUCCUUAUUAAAAUUAGAUUGCUAAAAAUAACCAUAAAAGAAAGUGACUUCAUUACCUCUAUCUAAUUUUAGCAAAACUAUCUAAAAUAUUCAAGAUAACAAUAUAUUCUUUCCAGAAGAAGACUAUUAUCAAAUUAUAAAAAAAUGAUUACCUAAUAAAAAUUUUUAAAGUUUUUUUAAUUUAAUUAUUGUUCUUGC ...................((((..(((((((........((((((.(((....(.((((((.((((((..((((((....((.((.(((((......))))))).)).......((((((((((((((.........))))))))))))))))))))..))))))))))))).....................((((((((((((((.((...(((..((..((((....))))..)).))).)).)))))))).....))))))........)))....)))))).(((((......)))))...)))))))..........((((((((......(((((((.....)))))))......))))))))....)))) (-49.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCAAGAACAAUAAUUAAAUUAAAAAAACUUUAAAAAUUUUUAUUAGGUAAUCAUUUUUUUAUAAUUUGAUAAUAGUCUUCUUCUGGAAAGAAUAUAUUGUUAUCUUGAAUAUUUUAGAUAGUUUUGCUAAAAUUAGAUAGAGGUAAUGAAGUCACUUUCUUUUAUGGUUAUUUUUAGCAAUCUAAUUUUAAUAAGGAAGUUUGAUUGUCUCAAUAUUUUUUAUUUAAAAAUGAUAUUGAUAGAAAUUUUUUAUCAACAUCAUUUAUUAUUUAACUCUAAUUUUUUAUAAUUAUGACUUUAAAUUAAACUUAAAAUACUUGAGAGCUUUUAGAAAAUAUAAUAUUUUGUCAUCAAGUUUACUGAUUUUUAGGGGGAGUUU .........(((((((...(((((((....((((((....((((((..((((((((((((((.....(((((((((....((((....))))...))))))))).........((((((.....((((((((.((((((((((...((....))....)))))))..))).)))))))))))))).....)))))))))..)))))..)).)))).)))))).....(((((((.((((((((.....)))))))).)))))))...............))))))))))))))((((((...(((((..(.....((((((..((.....................))..))))))......)..)))))..)))))). (-63.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 714 50 1 141 92 NC_003454:FN1672|5end_Membrane-bound_O-acyltransferase_171293..171523_NEGATIVE 249 153 116 94 59 NC_003454:FN0812|5end_Hypothetical_cytosolic_protein_1471051..1471281_POSITIVE 247 270 230 217 178 NC_003454:FN2128|5end_RECF_protein_639585..639815_NEGATIVE 224 244 201 89 49 NC_003454:FN1848|5end_Metal_dependent_hydrolase_337112..337342_POSITIVE 207 240 191 99 54 NC_003454:FN0396|5end_Dipeptide-binding_protein_1034203..1034433_POSITIVE 198 152 116 200 162 NC_003454:FN0275|5end_Transposase_901560..901790_NEGATIVE 197 152 116 203 165 NC_003454:FN0599|5end_Transposase_1253833..1254063_POSITIVE 196 87 48 59 18 NC_003454:FN1012|5end_HPr_kinase/phosphorylase_1667527..1667757_NEGATIVE 195 300 260 75 37 NC_003454:FN0823|5end_GTP-binding_protein_hflX_1482367..1482597_POSITIVE 194 245 213 86 54 NC_003454:FN0386|5end_hypothetical_protein_1019045..1019275_POSITIVE 193 299 252 77 17 NC_003454:FN0656|5end_hypothetical_protein_1313725..1313955_POSITIVE 192 318 285 42 9 NC_003454:FN2068|5end_dGTP_triphosphohydrolase_578986..579216_NEGATIVE 191 155 111 189 135 NC_003454:FN0765|5end_tRNA_(5-methylaminomethyl-2-thiouridylate)_-methyltransferase_1420792..1421022_POSITIVE 191 265 224 41 3 NC_003454:FN1640|5end_LSU_ribosomal_protein_L22P_138517..138747_NEGATIVE 190 313 274 213 166 NC_003454:FN0609|5end_SsrA-binding_protein_1264261..1264491_POSITIVE 189 77 35 61 15 NC_003454:FN1811|5end_Manganese_transport_system_ATP-binding_protein_mntA_292850..293080_NEGATIVE 187 350 303 116 68 NC_003454:FN0532|5end_Hypothetical_membrane-spanning_protein_1187579..1187809_NEGATIVE 186 280 231 139 88 NC_003454:FN0519|5end_Hypothetical_exported_24-amino_acid_repeat_protein_1171669..1171899_NEGATIVE 186 50 4 55 1 NC_003454:FN1657|5end_SSU_ribosomal_protein_S6P_153122..153352_NEGATIVE 185 163 128 182 139 NC_003454:FN0484|5end_Lipase_1129470..1129700_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 152 111 71 28 NC_003454:FN0123|3end_ATPase_755027..755177_POSITIVE 287 155 111 69 24 NC_003454:FN1913|3end_hydrolase_(HD_superfamily)_410054..410204_NEGATIVE 260 159 113 90 43 NC_003454:FN0927|3end_hypothetical_protein_1581693..1581843_NEGATIVE 260 152 116 78 40 NC_003454:FN0120|3end_hypothetical_protein_752718..752868_NEGATIVE 260 159 114 48 1 NC_003454:FN0119|3end_Flavodoxin_752647..752797_POSITIVE 249 153 116 71 36 NC_003454:FN0811|3end_hypothetical_protein_1471108..1471258_POSITIVE 236 152 116 63 29 NC_003454:FN0049|3end_hypothetical_protein_686919..687069_POSITIVE 228 155 111 50 5 NC_003454:FN0254|3end_Fusobacterium_outer_membrane_protein_family_881390..881540_POSITIVE 224 244 201 105 65 NC_003454:FN1847|3end_DTDP-4-dehydrorhamnose_3,5-epimerase_337208..337358_POSITIVE 205 154 111 71 18 NC_003454:FN0556|3end_hypothetical_protein_1208380..1208530_NEGATIVE 195 300 260 54 16 NC_003454:FN0822|3end_Shikimate_kinase_1482426..1482576_POSITIVE 193 299 252 62 2 NC_003454:FN0655|3end_hypothetical_protein_1313790..1313940_POSITIVE 189 77 35 59 13 NC_003454:FN1812|3end_Manganese-binding_protein_292852..293002_NEGATIVE 188 251 224 142 115 NC_003454:FN1871|3end_hypothetical_protein_362297..362447_NEGATIVE 187 350 303 94 46 NC_003454:FN0533|3end_Hypothetical_membrane-spanning_protein_1187601..1187751_NEGATIVE 186 280 231 130 79 NC_003454:FN0520|3end_hypothetical_protein_1171678..1171828_NEGATIVE 185 64 24 143 93 NC_003454:FN2123|3end_Phenylalanyl-tRNA_synthetase_alpha_chain_632208..632358_NEGATIVE 183 75 47 79 53 NC_003454:FN1098|3end_hypothetical_protein_1743898..1744048_POSITIVE 183 148 109 84 41 NC_003454:FN0905|3end_hypothetical_protein_1563781..1563931_NEGATIVE 182 240 193 101 48 NC_003454:FN1307|3end_CDP-diacylglycerol--glycerol-3-phosphate_3-phosphatidyltransferase_1964056..1964206_POSITIVE