Origin IGS: aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttatacctcctcgtataataaatttttatttgacttttttatagtataatcatatactgacattatataaagtatcagaataaaacttttttatggagtcagatt Mask Tandem Repeat Region ================================================ aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Find is-nt database================================================ Query_seq: FN1462:FN1463|FN1462:FN1463:Transcriptional regulator, GntR family:pyridoxine biosynthesis protein:<-->:2145036..2145141 106 aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1462:FN1463|FN1462:FN1463:Transcriptional regulator, GntR family:pyridoxine biosynthesis protein:<-->:2145036..2145141 106 aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1462:FN1463|FN1462:FN1463:Transcriptional regulator, GntR family:pyridoxine biosynthesis protein:<-->:2145036..2145141 106 aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Predict ORF larger than 30AA ================================================ aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 48 End: 77 ...............................................TTTGACTTTTTTATAGTATAATCATATACT............................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN1462:FN1463|FN1462:FN1463:Transcriptional regulator, GntR family:pyridoxine biosynthesis protein:<-->:2145036..2145141 106 aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Intra-Species Hit: Count: 1 Min: 1 Max: 106 Len: 106 Subject: NC_003454_FN1462_FN1463|Transcriptional regulator, GntR family:pyridoxine biosynthesis protein|NEGATIVE:POSITIVE|[2145036,2145141]|106 HSP 1 e-value: 6.0E-54 bit: 210.0 Len: 106 Query Start:1 Query End:106 Subject Strand: POSITIVE Subject Start: 1 Subject End: 106 aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa aatctgactccataaaaaagttttattctgatactttatataatgtcagtatatgattatactataaaaaagtcaaataaaaatttattatacgaggaggtataaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCUGACUCCAUAAAAAAGUUUUAUUCUGAUACUUUAUAUAAUGUCAGUAUAUGAUUAUACUAUAAAAAAGUCAAAUAAAAAUUUAUUAUACGAGGAGGUAUAAA .......((((((((.(((.(((((((.((((..((((((....((((.....)))).....))))))...))))))))))).))).))))....))))....... (-15.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUAUACCUCCUCGUAUAAUAAAUUUUUAUUUGACUUUUUUAUAGUAUAAUCAUAUACUGACAUUAUAUAAAGUAUCAGAAUAAAACUUUUUUAUGGAGUCAGAUU ..........(((.(((((.(((.((((((((.(((((..(((((((((....)))))))....))...)))))....)))))))).))).))))))))....... (-15.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 700 50 1 141 92 NC_003454:FN1462|5end_Transcriptional_regulator,_GntR_family_2144945..2145175_NEGATIVE 215 63 24 137 99 NC_003454:FN1284|5end_30S_ribosomal_protein_S4_1943243..1943473_NEGATIVE 187 79 44 169 137 NC_003454:FN1796|5end_hypothetical_protein_278476..278706_POSITIVE 181 45 3 60 12 NC_003454:FN1576|5end_ATPase_and_helicase_subunits_involved_in_DNA_replication_87374..87604_NEGATIVE 174 50 8 143 97 NC_003454:FN0476|5end_transcription_termination_factor_Rho_1123450..1123680_POSITIVE 168 36 7 216 190 NC_003454:FN1236|5end_Choline_transport_protein_1893480..1893710_NEGATIVE 165 69 21 141 96 NC_003454:FN1609|5end_hypothetical_protein_119613..119843_NEGATIVE 164 89 43 83 32 NC_003454:FN1413|5end_translation_initiation_factor_IF-2B_subunit_alpha_2074270..2074500_NEGATIVE 163 48 1 65 16 NC_003454:FN0511|5end_D-lactate_dehydrogenase_1160285..1160515_POSITIVE 161 48 8 152 114 NC_003454:FN0494|5end_Short_chain_dehydrogenase_1138777..1139007_POSITIVE 160 48 8 90 52 NC_003454:FN0386|5end_hypothetical_protein_1019045..1019275_POSITIVE 159 80 32 193 140 NC_003454:FN1135|5end_Phosphonates-binding_protein_1782131..1782361_POSITIVE 159 48 2 138 95 NC_003454:FN1836|5end_Tetratricopeptide_repeat_family_protein_326714..326944_NEGATIVE 158 87 48 98 62 NC_003454:FN0457|5end_hypothetical_protein_1103298..1103528_POSITIVE 155 46 1 133 94 NC_003454:FN1312|5end_Biopolymer_transport_exbB_protein_1966948..1967178_NEGATIVE 152 70 21 139 86 NC_003454:FN1716|5end_hypothetical_protein_212476..212706_POSITIVE 151 48 8 120 86 NC_003454:FN1461|5end_Histidinol-phosphatase_2143444..2143674_NEGATIVE 150 38 4 95 55 NC_003454:FN0350|5end_hypothetical_protein_982372..982602_POSITIVE 148 95 56 129 90 NC_003454:FN0626|5end_Hypothetical_cytosolic_protein_1286092..1286322_NEGATIVE 148 50 3 224 173 NC_003454:FN1519|5end_23S_rRNA_methyltransferase_24792..25022_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 215 63 24 97 59 NC_003454:FN1285|3end_30S_ribosomal_protein_S11_1943283..1943433_NEGATIVE 181 45 3 57 9 NC_003454:FN1577|3end_Rod_shape-determining_protein_mreB_87377..87527_NEGATIVE 174 50 8 120 74 NC_003454:FN0475|3end_MIAB_protein_1123507..1123657_POSITIVE 164 89 43 53 2 NC_003454:FN1414|3end_NA+/H+_antiporter_NHAC_2074300..2074450_NEGATIVE 159 48 2 127 84 NC_003454:FN1837|3end_putative_nucleotide-binding_protein_326725..326875_NEGATIVE 158 87 48 62 26 NC_003454:FN0456|3end_Hypothetical_cytosolic_protein_1103342..1103492_POSITIVE 148 95 56 66 27 NC_003454:FN0627|3end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing]_1286155..1286305_NEGATIVE 144 95 54 56 4 NC_003454:FN0229|3end_hypothetical_protein_856325..856475_NEGATIVE 142 54 16 146 107 NC_003454:FN1885|3end_Hemolysin_III_371411..371561_POSITIVE 141 43 4 50 10 NC_003454:FN0977|3end_Cobyric_acid_synthase_1629090..1629240_NEGATIVE 138 48 5 43 5 NC_003454:FN1260|3end_Sensory_Transduction_Protein_Kinase_1915863..1916013_NEGATIVE 138 51 18 56 25 NC_003454:FN0718|3end_hypothetical_protein_1376004..1376154_POSITIVE 137 24 2 44 23 NC_003454:FN1042|3end_S1_RNA_binding_domain_1694229..1694379_POSITIVE 135 99 84 40 24 NC_003454:FN0192|3end_Dipeptide-binding_protein_818969..819119_POSITIVE 135 86 63 50 25 NC_003454:FN2055|3end_hypothetical_protein_564002..564152_NEGATIVE 134 22 4 78 63 NC_003454:FN1447|3end_Transposase_2117342..2117492_POSITIVE 134 22 4 77 62 NC_003454:FN1442|3end_Transposase_2110155..2110305_NEGATIVE 134 22 4 77 62 NC_003454:FN0841|3end_Transposase_1502804..1502954_POSITIVE 134 22 4 77 62 NC_003454:FN0787|3end_Transposase_1443908..1444058_POSITIVE 134 22 4 77 62 NC_003454:FN0486|3end_Transposase_1131710..1131860_POSITIVE