Origin IGS: gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttgggatttaatttcctaggattttttaaccattctacctttgaacatgcatatgaatatgtttttccattattactaaatttgaatttgacatccatcttttttgtatagtcattatacatacatgaaagtatgtcagatgtctttatttcatcattaataataatcatattgtattttgtatccattgttccttctc Mask Tandem Repeat Region ================================================ gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Find is-nt database================================================ Query_seq: FN1444:FN1445|FN1444:FN1445:GMP synthase [glutamine-hydrolyzing]:DNA helicase:->->:2113264..2113463 200 gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1444:FN1445|FN1444:FN1445:GMP synthase [glutamine-hydrolyzing]:DNA helicase:->->:2113264..2113463 200 gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1444:FN1445|FN1444:FN1445:GMP synthase [glutamine-hydrolyzing]:DNA helicase:->->:2113264..2113463 200 gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 61 End: 162 ............................................................ M T Y F H V C I M T I Q K R W M S N S N L V I M E K H I H M H V Q R ...................................... Protein_Len: 60 Strand: + Start: 12 End: 200 ........... M D T K Y N M I I I N D E I K T S D I L S C M Y N D Y T K K M D V K F K F S N N G K T Y S Y A C S K V E W L K N P R K L Protein_Len: 36 Strand: - Start: 92 End: 199 ........................................................................................... V F F I S T L N L N L L L P F V Y E Y A H E F T S H N F F G L F N F G M . gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 43 HP_score: -3.3 Tail_Score: -2.82015 Start: 65 End: 80 Full_Region: AATAAAGACATCTGA CATAC TTTCAT GTATG TATAATGACTATACA ................................................................CATACTTTCATGTATG........................................................................................................................ Find igs database================================================ Query_seq: FN1444:FN1445|FN1444:FN1445:GMP synthase [glutamine-hydrolyzing]:DNA helicase:->->:2113264..2113463 200 gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Intra-Species Hit: Count: 1 Min: 1 Max: 200 Len: 200 Subject: NC_003454_FN1444_FN1445|GMP synthase [glutamine-hydrolyzing]:DNA helicase|POSITIVE:POSITIVE|[2113264,2113463]|200 HSP 1 e-value: 1.0E-109 bit: 396.0 Len: 200 Query Start:1 Query End:200 Subject Strand: POSITIVE Subject Start: 1 Subject End: 200 gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac gagaaggaacaatggatacaaaatacaatatgattattattaatgatgaaataaagacatctgacatactttcatgtatgtataatgactatacaaaaaagatggatgtcaaattcaaatttagtaataatggaaaaacatattcatatgcatgttcaaaggtagaatggttaaaaaatcctaggaaattaaatcccaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGAAGGAACAAUGGAUACAAAAUACAAUAUGAUUAUUAUUAAUGAUGAAAUAAAGACAUCUGACAUACUUUCAUGUAUGUAUAAUGACUAUACAAAAAAGAUGGAUGUCAAAUUCAAAUUUAGUAAUAAUGGAAAAACAUAUUCAUAUGCAUGUUCAAAGGUAGAAUGGUUAAAAAAUCCUAGGAAAUUAAAUCCCAAC ....((((......(((......(((......(((((((((((...((((.....((((((...(((.((((..(((((((......)).)))))..)))))))))))))...))))...))))))))))).(((...((((....))))....)))....))).....)))......)))).(((.......))).... (-32.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUGGGAUUUAAUUUCCUAGGAUUUUUUAACCAUUCUACCUUUGAACAUGCAUAUGAAUAUGUUUUUCCAUUAUUACUAAAUUUGAAUUUGACAUCCAUCUUUUUUGUAUAGUCAUUAUACAUACAUGAAAGUAUGUCAGAUGUCUUUAUUUCAUCAUUAAUAAUAAUCAUAUUGUAUUUUGUAUCCAUUGUUCCUUCUC .((((((.......)))))).......................(((((((.(((..((...........(((((((.(((...((((...((((((((((((((.(((((.((......))))))).))))).)))...)))))).....))))...))).)))))))..........))..))).)).)))))...... (-34.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 205 90 49 225 180 NC_003454:FN1158|5end_hypothetical_protein_1814420..1814650_POSITIVE 193 198 158 181 149 NC_003454:FN0781|5end_Integral_membrane_protein_1438281..1438511_NEGATIVE 190 96 58 95 55 NC_003454:FN1575|5end_Hypothetical_cytosolic_protein_86495..86725_NEGATIVE 182 197 154 228 189 NC_003454:FN0764|5end_Transporter,_LysE_family_1420327..1420557_POSITIVE 182 197 154 70 31 NC_003454:FN0763|5end_Transporter,_LysE_family_1420169..1420399_POSITIVE 176 192 151 206 165 NC_003454:FN1354|5end_ABC_transporter_permease_protein_2006682..2006912_NEGATIVE 171 189 146 209 167 NC_003454:FN0319|5end_Citrate_(pro-3S)-lyase_ligase_956855..957085_NEGATIVE 166 200 153 52 5 NC_003454:FN0467|5end_murein_hydrolase_exporter_1112864..1113094_POSITIVE 165 184 141 126 85 NC_003454:FN0002|5end_Ribonuclease_P_protein_component_642548..642778_POSITIVE 163 189 142 77 25 NC_003454:FN0704|5end_hypothetical_protein_1361855..1362085_NEGATIVE 163 189 142 208 156 NC_003454:FN0703|5end_N-acyl-L-amino_acid_amidohydrolase_1361724..1361954_NEGATIVE 163 75 36 63 27 NC_003454:FN1900|5end_pfoS/R_388244..388474_POSITIVE 162 115 84 142 113 NC_003454:FN0632|5end_PTS_system,_IIA_component_1290776..1291006_NEGATIVE 162 48 2 176 124 NC_003454:FN1649|5end_Oligopeptide_transport_ATP-binding_protein_oppD_144115..144345_NEGATIVE 161 165 122 197 156 NC_003454:FN0649|5end_Exoenzyme_regulatory_protein_aepA_precursor_1307717..1307947_NEGATIVE 160 186 150 142 113 NC_003454:FN0882|5end_Hemin_transport_system_ATP-binding_protein_hmuV_1543717..1543947_NEGATIVE 160 55 12 175 131 NC_003454:FN0375|5end_Iron(III)-binding_protein_1005293..1005523_POSITIVE 159 199 158 155 106 NC_003454:FN0307|5end_Iron(III)_dicitrate_transport_ATP-binding_protein_fecE_942095..942325_POSITIVE 158 190 145 225 180 NC_003454:FN0278|5end_Xaa-His_dipeptidase_905664..905894_NEGATIVE 154 164 138 26 1 NC_003454:FN1430|5end_Branched-chain_amino_acid_transport_system_permease_protein_livM_2099202..2099432_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 190 96 58 85 45 NC_003454:FN1576|3end_ATPase_and_helicase_subunits_involved_in_DNA_replication_86505..86655_NEGATIVE 181 77 42 58 23 NC_003454:FN0556|3end_hypothetical_protein_1208380..1208530_NEGATIVE 169 170 126 64 19 NC_003454:FN1144|3end_hypothetical_protein_1793403..1793553_NEGATIVE 164 110 61 79 30 NC_003454:FN1526|3end_Fusobacterium_outer_membrane_protein_family_31752..31902_NEGATIVE 160 55 12 70 26 NC_003454:FN0374|3end_Single-stranded-DNA-specific_exonuclease_recJ_1005268..1005418_POSITIVE 158 78 55 113 93 NC_003454:FN0773|3end_hypothetical_protein_1429635..1429785_POSITIVE 156 157 138 126 110 NC_003454:FN2126|3end_DNA_gyrase_subunit_B_636306..636456_NEGATIVE 153 81 47 65 35 NC_003454:FN0805|3end_hypothetical_protein_1464832..1464982_NEGATIVE 152 94 69 67 40 NC_003454:FN0325|3end_50S_ribosomal_protein_L20_960924..961074_NEGATIVE 151 68 49 71 53 NC_003454:FN2104|3end_hypothetical_protein_614760..614910_POSITIVE 150 171 132 73 30 NC_003454:FN2004|3end_Cobalt_transport_ATP-binding_protein_cbiO_505177..505327_POSITIVE 148 200 155 76 29 NC_003454:FN0467|3end_murein_hydrolase_exporter_1113300..1113450_POSITIVE 147 187 161 31 10 NC_003454:FN1797|3end_Spermidine/putrescine_transport_ATP-binding_protein_potA_280031..280181_POSITIVE 145 88 42 148 95 NC_003454:FN1438|3end_hypothetical_protein_2106541..2106691_POSITIVE 145 170 150 144 126 NC_003454:FN0884|3end_Hemin_transport_system_permease_protein_hmuU_1543704..1543854_NEGATIVE 144 189 160 66 31 NC_003454:FN1045|3end_hypothetical_protein_1695178..1695328_NEGATIVE 143 131 91 58 12 NC_003454:FN1444|3end_GMP_synthase_[glutamine-hydrolyzing]_2113203..2113353_POSITIVE 143 182 141 52 20 NC_003454:FN1347|3end_Hypothetical_cytosolic_protein_1999644..1999794_NEGATIVE 143 168 130 68 35 NC_003454:FN0402|3end_Integrase/recombinase_1040564..1040714_POSITIVE 141 185 148 34 5 NC_003454:FN0948|3end_Cobalt-zinc-cadmium_resistance_protein_czcD_1601377..1601527_NEGATIVE