Origin IGS: aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataaataagaaaattagaggagatatttttctcctctttttttgttttttataaaataatgaataaattttattgacaaaatactaaaatatggtttataatataatagctatgttaattatagaacatattttaataaattgcgtcactaacactagtgtatatagattattatttcccagaaagaaggtatagataaccttcttttttcttttcatatcaaaattaaattaatttaatgttttaaataaaaaatttaaaaaaattattttctttataatattataattaatataaaactaatatatgttatatattttactatataaatattatatagtaagcacaaaaggtactctaaaaatcatcatctatt Mask Tandem Repeat Region ================================================ aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Find is-nt database================================================ Query_seq: FN1425:FN1426|FN1425:FN1426:hypothetical protein:Serine protease:-><-:2092291..2092666 376 aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1425:FN1426|FN1425:FN1426:hypothetical protein:Serine protease:-><-:2092291..2092666 376 aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1425:FN1426|FN1425:FN1426:hypothetical protein:Serine protease:-><-:2092291..2092666 376 aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Predict ORF larger than 30AA ================================================ Protein_Len: 40 Strand: + Start: 159 End: 278 .............................................................................................................................................................. M K R K K K V I Y T F F L G N N N L Y T L V L V T Q F I K I C S I I N I A I I L .................................................................................................. Protein_Len: 34 Strand: - Start: 185 End: 286 ........................................................................................................................................................................................ V K K R P F L L R Y V S T N T V C N I L I H E I I L M A I I N Y V M .......................................................................................... aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 320 End: 349 ...............................................................................................................................................................................................................................................................................................................................TTTCTCCTCTTTTTTTGTTTTTTATAAAAT........................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 35 HP_score: -2.3 Tail_Score: -2.6062 Start: 48 End: 67 Full_Region: taaaactaatatatg ttatata ttttac tatataa atattatatagtaag ...............................................ttatatattttactatataa..................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 56 HP_score: -4.6 Tail_Score: -3.87075 Start: 173 End: 191 Full_Region: TATGAAAAGAAAAAA GAAGGT TATCTAT ACCTTC TTTCTGGGAAATAAT ............................................................................................................................................................................GAAGGTTATCTATACCTTC......................................................................................................................................................................................... TransTerm Strand: - Conf: 80 HP_score: -4.6 Tail_Score: -5.83211 Start: 173 End: 191 Full_Region: attatttcccagaaa gaaggt atagata accttc ttttttcttttcata ............................................................................................................................................................................gaaggtatagataaccttc......................................................................................................................................................................................... TransTerm Strand: + Conf: 70 HP_score: -7.9 Tail_Score: -3.35275 Start: 341 End: 360 Full_Region: TAAAAAACAAAAAAA GAGGAGA AAAATA TCTCCTC TAATTTTCTTATTTA ....................................................................................................................................................................................................................................................................................................................................................GAGGAGAAAAATATCTCCTC................ Find igs database================================================ Query_seq: FN1425:FN1426|FN1425:FN1426:hypothetical protein:Serine protease:-><-:2092291..2092666 376 aatagatgatgatttttagagtaccttttgtgcttactatataatatttatatagtaaaatatataacatatattagttttatattaattataatattataaagaaaataatttttttaaattttttatttaaaacattaaattaatttaattttgatatgaaaagaaaaaagaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagctattatattataaaccatattttagtattttgtcaataaaatttattcattattttataaaaaacaaaaaaagaggagaaaaatatctcctctaattttcttatttat Intra-Species Hit: Count: 1 Min: 1 Max: 376 Len: 376 Subject: NC_003454_FN1425_FN1426|hypothetical protein:Serine protease|POSITIVE:NEGATIVE|[2092291,2092666]|376 HSP 1 e-value: 2.0E-47 bit: 190.0 Len: 96 Query Start:173 Query End:268 Subject Strand: POSITIVE Subject Start: 173 Subject End: 268 ............................................................................................................................................................................gaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagc............................................................................................................ ............................................................................................................................................................................gaaggttatctataccttctttctgggaaataataatctatatacactagtgttagtgacgcaatttattaaaatatgttctataattaacatagc............................................................................................................ HSP 2 e-value: 4.0E-12 bit: 73.8 Len: 37 Query Start:1 Query End:37 Subject Strand: POSITIVE Subject Start: 1 Subject End: 37 aatagatgatgatttttagagtaccttttgtgcttac................................................................................................................................................................................................................................................................................................................................................... aatagatgatgatttttagagtaccttttgtgcttac................................................................................................................................................................................................................................................................................................................................................... HSP 3 e-value: 2.0E-11 bit: 71.9 Len: 36 Query Start:341 Query End:376 Subject Strand: POSITIVE Subject Start: 341 Subject End: 376 ....................................................................................................................................................................................................................................................................................................................................................gaggagaaaaatatctcctctaattttcttatttat ....................................................................................................................................................................................................................................................................................................................................................gaggagaaaaatatctcctctaattttcttatttat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAGAUGAUGAUUUUUAGAGUACCUUUUGUGCUUACUAUAUAAUAUUUAUAUAGUAAAAUAUAUAACAUAUAUUAGUUUUAUAUUAAUUAUAAUAUUAUAAAGAAAAUAAUUUUUUUAAAUUUUUUAUUUAAAACAUUAAAUUAAUUUAAUUUUGAUAUGAAAAGAAAAAAGAAGGUUAUCUAUACCUUCUUUCUGGGAAAUAAUAAUCUAUAUACACUAGUGUUAGUGACGCAAUUUAUUAAAAUAUGUUCUAUAAUUAACAUAGCUAUUAUAUUAUAAACCAUAUUUUAGUAUUUUGUCAAUAAAAUUUAUUCAUUAUUUUAUAAAAAACAAAAAAAGAGGAGAAAAAUAUCUCCUCUAAUUUUCUUAUUUAU .((((((((.((......(((((..((((((..((((((((((.....)))))))))).........(((((((((((..(((((..(((((.((((((.....(((.((((((((((((((.....))))))))....)))))).))).....)))))).........(((((((((.......)))))))))..........)))))..))))).))))))))..)))..((((..((((((((((((...((((((...............))))))...))))))))))))..))))..))))))..))))).......................((((((((......)))))))).....)))))))))) (-71.76) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAUAAGAAAAUUAGAGGAGAUAUUUUUCUCCUCUUUUUUUGUUUUUUAUAAAAUAAUGAAUAAAUUUUAUUGACAAAAUACUAAAAUAUGGUUUAUAAUAUAAUAGCUAUGUUAAUUAUAGAACAUAUUUUAAUAAAUUGCGUCACUAACACUAGUGUAUAUAGAUUAUUAUUUCCCAGAAAGAAGGUAUAGAUAACCUUCUUUUUUCUUUUCAUAUCAAAAUUAAAUUAAUUUAAUGUUUUAAAUAAAAAAUUUAAAAAAAUUAUUUUCUUUAUAAUAUUAUAAUUAAUAUAAAACUAAUAUAUGUUAUAUAUUUUACUAUAUAAAUAUUAUAUAGUAAGCACAAAAGGUACUCUAAAAAUCAUCAUCUAUU (((((.((.((((..((((((((......))))))))..)))).)).)))))....(((((((.....))))))).........(((((((((.((((((((.(((((....))))))))))))).)))))))))...........(((((....)))))...((((((.((.((((...(((((((((((.......)))))))))))(((((((((((.....(((.((((((((((((((.((((.(((((((((....))))).)))).)))).))))))).))))))).))).......)))))........(((((((((((.....)))))))))))...))))))........)))).)).)))))). (-72.30) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 525 230 181 185 135 NC_003454:FN1891|5end_Glycerophosphoryl_diester_phosphodiesterase_374294..374524_NEGATIVE 259 204 168 231 192 NC_003454:FN1448|5end_Hypothetical_cytosolic_protein_2118428..2118658_NEGATIVE 249 214 171 189 140 NC_003454:FN2046|5end_Acetyltransferase_551360..551590_NEGATIVE 227 367 338 155 124 NC_003454:FN0261|5end_Pyruvate_formate-lyase_activating_enzyme_887510..887740_NEGATIVE 208 278 243 35 2 NC_003454:FN1104|5end_Holliday_junction_DNA_helicase_ruvA_1749453..1749683_POSITIVE 202 42 1 51 15 NC_003454:FN0179|5end_Ankyrin_repeat_proteins_804790..805020_NEGATIVE 198 307 267 190 153 NC_003454:FN0514|5end_hypothetical_protein_1163431..1163661_POSITIVE 197 323 283 72 30 NC_003454:FN0959|5end_Precorrin-2_C20-methyltransferase_1615058..1615288_NEGATIVE 196 225 185 218 170 NC_003454:FN0628|5end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing]_1288257..1288487_NEGATIVE 191 203 181 223 204 NC_003454:FN0930|5end_Glycerol-3-phosphate_cytidylyltransferase_1583964..1584194_NEGATIVE 188 200 151 231 154 NC_003454:FN1553|5end_Transporter_68538..68768_NEGATIVE 186 369 331 177 143 NC_003454:FN2002|5end_Permease_503563..503793_NEGATIVE 186 198 151 146 95 NC_003454:FN1881|5end_Esterase_368639..368869_NEGATIVE 186 156 112 113 74 NC_003454:FN1765|5end_pyruvate_kinase_259450..259680_NEGATIVE 184 64 24 155 105 NC_003454:FN0592|5end_ATP-dependent_DNA_helicase_pcrA_1245712..1245942_POSITIVE 184 234 213 42 20 NC_003454:FN2010|5end_Transcriptional_regulator,_MarR_family_510302..510532_NEGATIVE 184 211 171 229 185 NC_003454:FN1597|5end_peptidyl-tRNA_hydrolase_107810..108040_NEGATIVE 183 199 152 108 59 NC_003454:FN0143|5end_hypothetical_protein_773897..774127_POSITIVE 179 196 175 64 41 NC_003454:FN1028|5end_deoxyuridine_5'-triphosphate_nucleotidohydrolase_1682383..1682613_NEGATIVE 179 38 16 178 146 NC_003454:FN0214|5end_Crossover_junction_endodeoxyribonuclease_ruvC_839240..839470_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 616 230 182 81 34 NC_003454:FN1554|3end_Fusobacterium_outer_membrane_protein_family_68719..68869_NEGATIVE 562 230 186 73 30 NC_003454:FN1449|3end_Fusobacterium_outer_membrane_protein_family_2118612..2118762_NEGATIVE 525 230 181 67 17 NC_003454:FN1893|3end_Fusobacterium_outer_membrane_protein_family_374412..374562_NEGATIVE 264 295 251 74 31 NC_003454:FN0059|3end_NifU_protein_695619..695769_POSITIVE 249 214 171 58 9 NC_003454:FN2047|3end_Fusobacterium_outer_membrane_protein_family_551491..551641_NEGATIVE 227 367 338 89 58 NC_003454:FN0262|3end_Formate_acetyltransferase_887576..887726_NEGATIVE 204 256 214 71 23 NC_003454:FN2010|3end_Transcriptional_regulator,_MarR_family_509820..509970_NEGATIVE 194 367 322 82 36 NC_003454:FN2120|3end_Hypothetical_exported_24-amino_acid_repeat_protein_628361..628511_NEGATIVE 184 211 171 60 16 NC_003454:FN1598|3end_hypothetical_protein_107979..108129_NEGATIVE 180 298 252 142 84 NC_003454:FN0131|3end_Hemolysin_activator_protein_precursor_761327..761477_POSITIVE 179 196 175 62 39 NC_003454:FN1029|3end_Zinc_protease_1682385..1682535_NEGATIVE 178 297 252 136 101 NC_003454:FN1352|3end_ABC_transporter_ATP-binding_protein_2003506..2003656_NEGATIVE 177 303 264 90 48 NC_003454:FN1927|3end_DEGV_protein_425415..425565_NEGATIVE 172 231 191 50 13 NC_003454:FN0665|3end_N-acetylmuramoyl-L-alanine_amidase_1321626..1321776_POSITIVE 169 327 288 82 44 NC_003454:FN0759|3end_Maf_protein_1417278..1417428_NEGATIVE 166 193 158 76 40 NC_003454:FN1655|3end_Hypothetical_cytosolic_protein_152516..152666_POSITIVE 164 39 10 56 26 NC_003454:FN1198|3end_Transporter_1857679..1857829_NEGATIVE 164 260 217 74 22 NC_003454:FN0561|3end_Proline_synthetase_associated_protein_1214906..1215056_POSITIVE 164 368 339 112 79 NC_003454:FN0488|3end_NAD-specific_glutamate_dehydrogenase_1134456..1134606_POSITIVE 163 302 261 117 75 NC_003454:FN0721|3end_hypothetical_protein_1377633..1377783_NEGATIVE