Origin IGS: aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaataatactctccttagtattctattatatttagatttttatacattaactacattatcttttagaaaatataattaatctaaatgactcaattttaaataattaaacaataattttttttaccaaaccaatataagctattt Mask Tandem Repeat Region ================================================ aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Find is-nt database================================================ Query_seq: FN1408:FN1409|FN1408:FN1409:Aminoacyl-histidine dipeptidase:Transporter:->->:2069005..2069148 144 aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1408:FN1409|FN1408:FN1409:Aminoacyl-histidine dipeptidase:Transporter:->->:2069005..2069148 144 aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1408:FN1409|FN1408:FN1409:Aminoacyl-histidine dipeptidase:Transporter:->->:2069005..2069148 144 aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Predict ORF larger than 30AA ================================================ aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN1408:FN1409|FN1408:FN1409:Aminoacyl-histidine dipeptidase:Transporter:->->:2069005..2069148 144 aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Intra-Species Hit: Count: 1 Min: 1 Max: 144 Len: 144 Subject: NC_003454_FN1408_FN1409|Aminoacyl-histidine dipeptidase:Transporter|POSITIVE:POSITIVE|[2069005, 2069148]|144 HSP 1 e-value: 4.0E-62 bit: 238.0 Len: 144 Query Start:1 Query End:144 Subject Strand: POSITIVE Subject Start: 1 Subject End: 144 aaatagcttatattggtttggtnnnnnnnnttattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt aaatagcttatattggtttggtaaaaaaaattattgtttaattatttaaaattgagtcatttagattaattatattttctaaaagataatgtagttaatgtataaaaatctaaatataatagaatactaaggagagtattattt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAGCUUAUAUUGGUUUGGUAAAAAAAAUUAUUGUUUAAUUAUUUAAAAUUGAGUCAUUUAGAUUAAUUAUAUUUUCUAAAAGAUAAUGUAGUUAAUGUAUAAAAAUCUAAAUAUAAUAGAAUACUAAGGAGAGUAUUAUUU .......((((((((..(..((..(((.(((((.....))))).)))...))..)..)...((.((((((((((((.((.....)).)))))))))))).))..........)))))))...((((((......)))))).... (-20.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUAAUACUCUCCUUAGUAUUCUAUUAUAUUUAGAUUUUUAUACAUUAACUACAUUAUCUUUUAGAAAAUAUAAUUAAUCUAAAUGACUCAAUUUUAAAUAAUUAAACAAUAAUUUUUUUUACCAAACCAAUAUAAGCUAUUU ....((((((......))))))......((((((((((.(((((......(((.(......).)))....)))))..))))))))))......................................................... ( -9.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 163 84 46 57 22 NC_003454:FN1133|5end_N-acetylglucosamine-6-phosphate_deacetylase_1781429..1781659_NEGATIVE 161 78 43 149 109 NC_003454:FN0871|5end_3-dehydroquinate_synthase_1531767..1531997_NEGATIVE 160 136 94 192 155 NC_003454:FN0944|5end_Na+_driven_multidrug_efflux_pump_1598855..1599085_NEGATIVE 159 68 31 56 21 NC_003454:FN0869|5end_Hydrolase_(HAD_superfamily)_1528920..1529150_NEGATIVE 158 80 39 35 1 NC_003454:FN0132|5end_Hemolysin_761715..761945_POSITIVE 155 110 61 132 84 NC_003454:FN1300|5end_Integral_membrane_protein_1956788..1957018_POSITIVE 155 139 102 225 184 NC_003454:FN0923|5end_Cardiolipin_synthetase_1578977..1579207_NEGATIVE 151 97 58 72 31 NC_003454:FN2085|5end_hypothetical_protein_597045..597275_NEGATIVE 151 50 4 77 32 NC_003454:FN2054|5end_glucose-6-phosphate_isomerase_562536..562766_POSITIVE 150 120 71 111 64 NC_003454:FN1830|5end_DNA_polymerase_III_subunits_gamma_and_tau_320025..320255_NEGATIVE 148 140 91 211 152 NC_003454:FN1413|5end_translation_initiation_factor_IF-2B_subunit_alpha_2074270..2074500_NEGATIVE 148 96 51 213 166 NC_003454:FN0630|5end_PTS_system,_IIC_component_1289857..1290087_NEGATIVE 148 48 6 189 146 NC_003454:FN1999|5end_HIPA_protein_500262..500492_POSITIVE 148 48 6 56 13 NC_003454:FN1998|5end_HIPA_protein_500129..500359_POSITIVE 148 63 26 138 97 NC_003454:FN1893|5end_Fusobacterium_outer_membrane_protein_family_378497..378727_NEGATIVE 148 87 50 36 2 NC_003454:FN1855|5end_Methylaspartate_mutase_343949..344179_POSITIVE 147 60 11 222 173 NC_003454:FN0972|5end_Cobyrinic_acid_a,c-diamide_synthase_1625607..1625837_NEGATIVE 144 87 53 33 1 NC_003454:FN1450|5end_Integral_membrane_protein_2131410..2131640_NEGATIVE 143 111 73 214 174 NC_003454:FN1244|5end_Polysaccharide_deacetylase_1901241..1901471_NEGATIVE 142 104 61 179 142 NC_003454:FN1970|5end_Hemin-binding_periplasmic_protein_hmuT_precursor_469056..469286_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 162 138 109 63 34 NC_003454:FN0536|3end_DNA_polymerase_III,_beta_chain_1190006..1190156_NEGATIVE 161 78 43 42 2 NC_003454:FN0872|3end_hypothetical_protein_1531874..1532024_NEGATIVE 159 68 31 63 28 NC_003454:FN0870|3end_Rhodanese-related_sulfurtransferase_1528913..1529063_NEGATIVE 150 120 71 107 60 NC_003454:FN1831|3end_Nitrogen_assimilation_regulatory_protein_320029..320179_NEGATIVE 142 104 61 102 65 NC_003454:FN1971|3end_Hemin_receptor_469133..469283_NEGATIVE 141 47 5 60 6 NC_003454:FN1978|3end_Cell_division_protein_ftsH_481703..481853_POSITIVE 140 97 51 83 38 NC_003454:FN1620|3end_30S_ribosomal_protein_S2_128719..128869_POSITIVE 139 122 84 67 34 NC_003454:FN1744|3end_Transporter_242039..242189_NEGATIVE 138 138 99 50 14 NC_003454:FN0047|3end_Exodeoxyribonuclease_III_685268..685418_POSITIVE 136 106 64 70 25 NC_003454:FN1108|3end_Phosphohydrolase_(MUTT/NUDIX_family_protein)_1752878..1753028_POSITIVE 136 46 6 53 14 NC_003454:FN0753|3end_aspartyl/glutamyl-tRNA_amidotransferase_subunit_B_1411724..1411874_NEGATIVE 136 79 31 75 29 NC_003454:FN2041|3end_Transcription_antitermination_protein_nusG_549078..549228_NEGATIVE 135 81 51 41 11 NC_003454:FN1036|3end_hypothetical_protein_1689478..1689628_POSITIVE 135 38 1 48 5 NC_003454:FN1798|3end_Spermidine/putrescine_transport_system_permease_protein_potB_280872..281022_POSITIVE 133 39 8 80 55 NC_003454:FN1707|3end_Aldose_1-epimerase_203268..203418_POSITIVE 131 102 75 54 26 NC_003454:FN0384|3end_hypothetical_protein_1017969..1018119_POSITIVE 131 96 57 151 107 NC_003454:FN1809|3end_Iron/zinc/copper-binding_protein_290180..290330_NEGATIVE 130 46 4 150 106 NC_003454:FN1299|3end_dTDP-glucose_4,6-dehydratase_1955695..1955845_NEGATIVE 129 81 52 38 7 NC_003454:FN1226|3end_uracil-DNA_glycosylase_1884823..1884973_NEGATIVE 129 137 97 65 24 NC_003454:FN0480|3end_hypothetical_protein_1127839..1127989_POSITIVE