Origin IGS: ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tattaaatgtggtaaagacttcattgctagctaaatttattgatactatgaggagaatagctgtgaatattaaggagtttaagggaaataaaaaagaatttcttttattgcttttacttgcagatgaaaaagaagaa Mask Tandem Repeat Region ================================================ ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Find is-nt database================================================ Query_seq: FN1384:FN1385|FN1384:FN1385:IAA acetyltransferase:hypothetical protein:<-<-:2038347..2038483 137 ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1384:FN1385|FN1384:FN1385:IAA acetyltransferase:hypothetical protein:<-<-:2038347..2038483 137 ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1384:FN1385|FN1384:FN1385:IAA acetyltransferase:hypothetical protein:<-<-:2038347..2038483 137 ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Predict ORF larger than 30AA ================================================ Protein_Len: 43 Strand: - Start: 1 End: 129 E E K E D A L L L L L L F E K K N G K F E K I N V A I R R M T D I F K A L L S T K V M ........ ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN1384:FN1385|FN1384:FN1385:IAA acetyltransferase:hypothetical protein:<-<-:2038347..2038483 137 ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: NC_003454_FN1384_FN1385|IAA acetyltransferase:hypothetical protein|NEGATIVE:NEGATIVE|[2038347,2038483]|137 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata ttcttctttttcatctgcaagtaaaagcaataaaagaaattcttttttatttcccttaaactccttaatattcacagctattctcctcatagtatcaataaatttagctagcaatgaagtctttaccacatttaata Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUUCUUUUUCAUCUGCAAGUAAAAGCAAUAAAAGAAAUUCUUUUUUAUUUCCCUUAAACUCCUUAAUAUUCACAGCUAUUCUCCUCAUAGUAUCAAUAAAUUUAGCUAGCAAUGAAGUCUUUACCACAUUUAAUA ........((((((.(((.(((.(((..(((((((((.....))))))))).........................(((((.......))))).........))).))).))))))))).................. (-14.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUAAAUGUGGUAAAGACUUCAUUGCUAGCUAAAUUUAUUGAUACUAUGAGGAGAAUAGCUGUGAAUAUUAAGGAGUUUAAGGGAAAUAAAAAAGAAUUUCUUUUAUUGCUUUUACUUGCAGAUGAAAAAGAAGAA ..(((..((..(.......(((((.((((.((...(((((.......))))).))..)))).))))).....((((((.((((((((((........))))))))))..))))))..)..))..))).......... (-22.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 805 120 71 211 162 NC_003454:FN1384|5end_IAA_acetyltransferase_2038256..2038486_NEGATIVE 263 100 53 117 58 NC_003454:FN0087|5end_Ethanolamine_utilization_protein_eutN_724626..724856_POSITIVE 260 88 49 104 70 NC_003454:FN0716|5end_Phophatidylinositol-4-phosphate_5-kinase_1373969..1374199_POSITIVE 243 35 10 122 98 NC_003454:FN0774|5end_Hypothetical_cytosolic_protein_1429692..1429922_POSITIVE 237 60 19 128 76 NC_003454:FN1251|5end_High-affinity_iron_permease_1908008..1908238_POSITIVE 234 87 48 217 183 NC_003454:FN0133|5end_hypothetical_protein_769121..769351_POSITIVE 230 99 51 116 65 NC_003454:FN1352|5end_ABC_transporter_ATP-binding_protein_2004180..2004410_NEGATIVE 224 100 61 59 19 NC_003454:FN1808|5end_hypothetical_protein_289905..290135_NEGATIVE 222 47 1 210 164 NC_003454:FN0145|5end_hypothetical_protein_774306..774536_POSITIVE 221 49 1 75 14 NC_003454:FN1305|5end_Hypothetical_cytosolic_protein_1961205..1961435_POSITIVE 221 48 1 85 47 NC_003454:FN1024|5end_DNA-binding_protein_HU_1678480..1678710_NEGATIVE 221 98 51 159 97 NC_003454:FN1671|5end_ABC_transporter_ATP-binding_protein_169820..170050_NEGATIVE 220 119 89 47 21 NC_003454:FN1706|5end_Transporter_201433..201663_POSITIVE 216 118 75 95 48 NC_003454:FN0797|5end_hypothetical_protein_1455799..1456029_NEGATIVE 214 86 41 73 30 NC_003454:FN0231|5end_hypothetical_protein_857351..857581_NEGATIVE 214 86 41 207 164 NC_003454:FN0230|5end_hypothetical_protein_857217..857447_NEGATIVE 212 40 1 43 2 NC_003454:FN0668|5end_High-affinity_zinc_uptake_system_protein_znuA_precursor_1323969..1324199_POSITIVE 209 89 41 106 61 NC_003454:FN1580|5end_2-C-methyl-D-erythritol_4-phosphate_cytidylyltransferase_90919..91149_NEGATIVE 208 70 40 107 76 NC_003454:FN0944|5end_Na+_driven_multidrug_efflux_pump_1598855..1599085_NEGATIVE 208 127 82 101 54 NC_003454:FN1605|5end_Adenylosuccinate_synthetase_115276..115506_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 263 100 53 115 56 NC_003454:FN0086|3end_hypothetical_protein_724704..724854_POSITIVE 260 88 49 111 77 NC_003454:FN0715|3end_hypothetical_protein_1374056..1374206_POSITIVE 230 99 51 112 61 NC_003454:FN1353|3end_ABC_transporter_permease_protein_2004184..2004334_NEGATIVE 230 109 62 55 12 NC_003454:FN0950|3end_Precorrin-6X_reductase_1606975..1607125_NEGATIVE 227 130 100 61 28 NC_003454:FN0046|3end_3-dehydroquinate_dehydratase_684265..684415_POSITIVE 222 47 1 58 12 NC_003454:FN0143|3end_hypothetical_protein_774234..774384_POSITIVE 221 98 51 146 84 NC_003454:FN1672|3end_Membrane-bound_O-acyltransferase_169833..169983_NEGATIVE 220 119 89 31 5 NC_003454:FN1704|3end_Serine_protease_201497..201647_POSITIVE 217 118 84 72 42 NC_003454:FN1218|3end_hypothetical_protein_1877812..1877962_NEGATIVE 215 118 100 54 36 NC_003454:FN0129|3end_Urease_accessory_protein_ureG_758370..758520_POSITIVE 215 118 100 87 69 NC_003454:FN2005|3end_Cobalt_transport_ATP-binding_protein_cbiO_505976..506126_POSITIVE 209 89 41 130 85 NC_003454:FN1581|3end_DNA_mismatch_repair_protein_mutS_90895..91045_NEGATIVE 208 127 82 91 44 NC_003454:FN1606|3end_3-deoxy-D-manno-octulosonic-acid_transferase_115286..115436_NEGATIVE 206 117 100 72 55 NC_003454:FN1811|3end_Manganese_transport_system_ATP-binding_protein_mntA_292161..292311_NEGATIVE 200 87 51 53 13 NC_003454:FN1091|3end_Sigma_factor_sigB_regulation_protein_rsbU_1737338..1737488_POSITIVE 200 87 42 108 56 NC_003454:FN0595|3end_UDP-N-acetylglucosamine_acyltransferase_1250081..1250231_POSITIVE 199 65 38 85 51 NC_003454:FN1294|3end_Ribosomal-protein-alanine_acetyltransferase_1952517..1952667_NEGATIVE 199 65 33 86 54 NC_003454:FN1269|3end_Hypothetical_lipoprotein_1925613..1925763_NEGATIVE 198 106 68 151 113 NC_003454:FN1156|3end_Primosomal_protein_N'_1813973..1814123_POSITIVE 198 101 62 148 100 NC_003454:FN0488|3end_NAD-specific_glutamate_dehydrogenase_1134456..1134606_POSITIVE