Origin IGS: atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gaaaaataataaagaaagagttttagggaatagaaaacttaaaactcttttttgattaataaagttgttaaaattttatttttatgatgttatgtgtatatacatgatagagaagggaaatgatacacatgaaagaaatatatcctaaatgaatgctattgttttatattaagaaatattgcttcatcaaaatgaaaatgaaagtgccacttttactctctattcttattttttaaaaggcgttaatgttgacaaaaagcgtgacatgtactgcacttccattcttgtgtccaagattttgggtgcagtacaaataaat Mask Tandem Repeat Region ================================================ atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Find is-nt database================================================ Query_seq: FN1221:FN1222|FN1221:FN1222:hypothetical protein:hypothetical protein:-><-:1881351..1881669 319 atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN1221:FN1222|FN1221:FN1222:hypothetical protein:hypothetical protein:-><-:1881351..1881669 319 atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN1221:FN1222|FN1221:FN1222:hypothetical protein:hypothetical protein:-><-:1881351..1881669 319 atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Predict ORF larger than 30AA ================================================ Protein_Len: 48 Strand: - Start: 1 End: 144 N I Q V A G L I K S V L I S T C Y M D R K K D V N V G K L F Y S Y L T F T A S E N E N Q H L L M ............................................................................................................................................................................... atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN1221:FN1222|FN1221:FN1222:hypothetical protein:hypothetical protein:-><-:1881351..1881669 319 atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Intra-Species Hit: Count: 1 Min: 1 Max: 319 Len: 319 Subject: NC_003454_FN1221_FN1222|hypothetical protein:hypothetical protein|POSITIVE:NEGATIVE|[1881351,1881669]|319 HSP 1 e-value: 1.0E-180 bit: 632.0 Len: 319 Query Start:1 Query End:319 Subject Strand: POSITIVE Subject Start: 1 Subject End: 319 atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc atttatttgtactgcacccaaaatcttggacacaagaatggaagtgcagtacatgtcacgctttttgtcaacattaacgccttttaaaaaataagaatagagagtaaaagtggcactttcattttcattttgatgaagcaatatttcttaatataaaacaatagcattcatttaggatatatttctttcatgtgtatcatttcccttctctatcatgtatatacacataacatcataaaaataaaattttaacaactttattaatcaaaaaagagttttaagttttctattccctaaaactctttctttattatttttc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUAUUUGUACUGCACCCAAAAUCUUGGACACAAGAAUGGAAGUGCAGUACAUGUCACGCUUUUUGUCAACAUUAACGCCUUUUAAAAAAUAAGAAUAGAGAGUAAAAGUGGCACUUUCAUUUUCAUUUUGAUGAAGCAAUAUUUCUUAAUAUAAAACAAUAGCAUUCAUUUAGGAUAUAUUUCUUUCAUGUGUAUCAUUUCCCUUCUCUAUCAUGUAUAUACACAUAACAUCAUAAAAAUAAAAUUUUAACAACUUUAUUAAUCAAAAAAGAGUUUUAAGUUUUCUAUUCCCUAAAACUCUUUCUUUAUUAUUUUUC .(((((((((((((((((((...(((((....))))).)))..))))))))).((((((((((((((((.........................).)))))))))....))))))...............(((((.((.(((((.((((((....................)))))).))))).))...(((((((((((..............)))...))))))))..)))))...)))))))................((((.((.(((((((((((..............))))))))))).)).))))...... (-57.54) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAAAAAUAAUAAAGAAAGAGUUUUAGGGAAUAGAAAACUUAAAACUCUUUUUUGAUUAAUAAAGUUGUUAAAAUUUUAUUUUUAUGAUGUUAUGUGUAUAUACAUGAUAGAGAAGGGAAAUGAUACACAUGAAAGAAAUAUAUCCUAAAUGAAUGCUAUUGUUUUAUAUUAAGAAAUAUUGCUUCAUCAAAAUGAAAAUGAAAGUGCCACUUUUACUCUCUAUUCUUAUUUUUUAAAAGGCGUUAAUGUUGACAAAAAGCGUGACAUGUACUGCACUUCCAUUCUUGUGUCCAAGAUUUUGGGUGCAGUACAAAUAAAU (((((((((..((((((((((((((((..........)))))))))))))))).......(((((.((.....((((((((((((....((((((((((...(((..............)))))))))))))................(((((.((...((((((.......))))))..)))))))....))))))))))))..)).)))))............))))))))).......((((.((((.......)))))))).((((((((((..(((.(((((....)))))...)))))))))))))....... (-74.14) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 621 50 8 71 29 NC_003454:FN0839|5end_hypothetical_protein_1501426..1501656_NEGATIVE 583 50 7 201 158 NC_003454:FN1444|5end_GMP_synthase_[glutamine-hydrolyzing]_2111585..2111815_POSITIVE 380 59 11 141 98 NC_003454:FN0135|5end_Transposase_770238..770468_POSITIVE 374 50 1 137 82 NC_003454:FN1443|5end_Transposase_2111518..2111748_NEGATIVE 374 50 1 137 82 NC_003454:FN0786|5end_Transposase_1442465..1442695_POSITIVE 370 56 11 228 183 NC_003454:FN1972|5end_hypothetical_protein_472071..472301_NEGATIVE 351 59 11 149 97 NC_003454:FN1887|5end_Transposase_371671..371901_POSITIVE 350 58 11 144 96 NC_003454:FN0840|5end_Transposase_1501361..1501591_POSITIVE 333 53 11 138 96 NC_003454:FN0485|5end_Transposase_1130267..1130497_POSITIVE 330 59 11 140 85 NC_003454:FN1446|5end_Transposase_2116033..2116263_POSITIVE 330 50 6 208 165 NC_003454:FN0210|5end_Transcriptional_regulator,_COPG_family_836998..837228_POSITIVE 252 204 168 104 63 NC_003454:FN0843|5end_hypothetical_protein_1503386..1503616_NEGATIVE 242 216 180 121 80 NC_003454:FN0289|5end_hypothetical_protein_916812..917042_NEGATIVE 241 140 93 177 119 NC_003454:FN1622|5end_Uridylate_kinase_129640..129870_POSITIVE 237 208 163 106 62 NC_003454:FN1743|5end_Multidrug-efflux_transporter_2_regulator_241838..242068_NEGATIVE 225 209 164 101 57 NC_003454:FN0755|5end_Glutamyl-tRNA(Gln)_amidotransferase_subunit_C_1414953..1415183_NEGATIVE 225 167 121 76 23 NC_003454:FN1971|5end_Hemin_receptor_471106..471336_NEGATIVE 224 218 175 93 58 NC_003454:FN1480|5end_MG2+_transporter_MGTE_2162663..2162893_NEGATIVE 220 50 12 114 75 NC_003454:FN0068|5end_Lipoprotein_signal_peptidase_707308..707538_POSITIVE 219 110 63 74 22 NC_003454:FN0327|5end_Bacterial_Protein_Translation_Initiation_Factor_3_(IF-3)_962149..962379_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 711 56 11 49 4 NC_003454:FN1019|3end_3-hydroxybutyryl-CoA_dehydrogenase_1672945..1673095_NEGATIVE 674 50 8 88 46 NC_003454:FN1904|3end_Transcriptional_regulator,_MarR_family_393633..393783_POSITIVE 568 50 11 81 42 NC_003454:FN1463|3end_pyridoxine_biosynthesis_protein_2145924..2146074_POSITIVE 471 50 11 79 40 NC_003454:FN1499|3end_Cell_surface_protein_5166..5316_POSITIVE 427 54 11 81 38 NC_003454:FN1498|3end_Integral_membrane_protein_3542..3692_POSITIVE 399 54 11 87 44 NC_003454:FN1905|3end_168_kDa_surface-layer_protein_precursor_393653..393803_NEGATIVE 379 50 11 67 29 NC_003454:FN0177|3end_Cell_division_inhibitor_MinE_802336..802486_POSITIVE 370 56 11 69 24 NC_003454:FN1973|3end_Translation_initiation_inhibitor_472230..472380_NEGATIVE 361 50 11 83 44 NC_003454:FN1143|3end_glucosamine-6-phosphate_deaminase_1793365..1793515_POSITIVE 360 53 11 78 36 NC_003454:FN1547|3end_PTS_permease_for_N-acetylglucosamine_and_glucose_61801..61951_NEGATIVE 350 50 8 80 38 NC_003454:FN1221|3end_hypothetical_protein_1881290..1881440_POSITIVE 337 50 11 80 41 NC_003454:FN1464|3end_1-deoxy-D-xylulose-5-phosphate_synthase_2145954..2146104_NEGATIVE 333 53 11 63 21 NC_003454:FN0484|3end_Lipase_1130272..1130422_POSITIVE 333 50 8 74 32 NC_003454:FN0235|3end_ABC_transporter_ATP-binding_protein_859674..859824_NEGATIVE 325 58 11 151 100 NC_003454:FN1886|3end_hypothetical_protein_371754..371904_POSITIVE 314 50 11 76 37 NC_003454:FN1546|3end_translation_elongation_factor_EF-G_59529..59679_NEGATIVE 313 56 11 76 31 NC_003454:FN0178|3end_UNC-44_ankyrins_802390..802540_NEGATIVE 274 50 11 79 39 NC_003454:FN1719|3end_hypothetical_protein_218747..218897_POSITIVE 262 50 11 85 46 NC_003454:FN1500|3end_Nickel_transport_ATP-binding_protein_nikE_5193..5343_NEGATIVE 251 32 5 82 55 NC_003454:FN1442|3end_Transposase_2110155..2110305_NEGATIVE