Origin IGS: aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttttttacctcactttttttgaataaaataatctccataattatagcattttttcaattaaatttctatttcaataattttatttctttagctatttacaaaagtaaataaatagtgtattatatttataaataattttaataaaaattcgtataagcctaatatatggaagggtgtccctacggttaaccataaattaaccagctacgaaaaatgttttactgtgtttcttttttaatatggaaatatattttttgtatgcttaacaaaaaggaggaaacagtgaaattaataactaaaaaaaatttaaataaaataaaatttaaggtagctgttttctacgggatagttgcgaacagttgtaattgtaaattataatctgagtgttttgctcagatttttttattttttaactatattataagctatt Mask Tandem Repeat Region ================================================ aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Find is-nt database================================================ Query_seq: FN0990:FN0991|FN0990:FN0991:Phosphoribosylformylglycinamidine synthase:CDP-diacylglycerol--serine O-phosphatidyltransferase:<-->:1645519..1645949 431 aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0990:FN0991|FN0990:FN0991:Phosphoribosylformylglycinamidine synthase:CDP-diacylglycerol--serine O-phosphatidyltransferase:<-->:1645519..1645949 431 aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0990:FN0991|FN0990:FN0991:Phosphoribosylformylglycinamidine synthase:CDP-diacylglycerol--serine O-phosphatidyltransferase:<-->:1645519..1645949 431 aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 114 End: 209 ................................................................................................................. M L F K F F L V I N F T V S S F L L S I Q K I Y F H I K K E T Q .............................................................................................................................................................................................................................. Protein_Len: 33 Strand: + Start: 238 End: 336 ............................................................................................................................................................................................................................................. M V N R R D T L P Y I R L I R I F I K I I Y K Y N T L F I Y F C K ............................................................................................... Protein_Len: 38 Strand: + Start: 227 End: 340 .................................................................................................................................................................................................................................. M V N L W L T V G T P F H I L G L Y E F L L K L F I N I I H Y L F T F V N S ........................................................................................... Protein_Len: 30 Strand: - Start: 323 End: 412 .................................................................................................................................................................................................................................................................................................................................. K S K Y I A L S I F N N F Y F N L Q F F A I I I S I I K N M ................... Protein_Len: 39 Strand: - Start: 196 End: 312 ................................................................................................................................................................................................... F L F V T F C K E Y S T L K H N V T P V G K W I N P K Y S N K N F N N I F I M ....................................................................................................................... Protein_Len: 60 Strand: - Start: 56 End: 235 ....................................................... L K C N C S N A V I G Y F V A V K F K I K N L N K K T I L K V T E E K K N L M C F I Y K W I L F S V C Y F M K R L Q N M .................................................................................................................................................................................................... aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 85 Start: 362 End: 391 .........................................................................................................................................................................................................................................................................................................................................................................ATAGAAATTTAATTGAAAAAATGCTATAAT........................................ PromScan Matrix: RpoD-17 Strand: - Score: 84 Start: 308 End: 337 ...................................................................................................................................................................................................................................................................................................................ATTTACAAAAGTAAATAAATAGTGTATTAT.............................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -6.0 Tail_Score: -6.28401 Start: 34 End: 52 Full_Region: aattgtaaattataa tctgagtg ttt tgctcaga tttttttatttttta .................................tctgagtgttttgctcaga........................................................................................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: FN0990:FN0991|FN0990:FN0991:Phosphoribosylformylglycinamidine synthase:CDP-diacylglycerol--serine O-phosphatidyltransferase:<-->:1645519..1645949 431 aatagcttataatatagttaaaaaataaaaaaatctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaaattttattttatttaaattttttttagttattaatttcactgtttcctcctttttgttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttccatatattaggcttatacgaatttttattaaaattatttataaatataatacactatttatttacttttgtaaatagctaaagaaataaaattattgaaatagaaatttaattgaaaaaatgctataattatggagattattttattcaaaaaaagtgaggtaaaaaaa Intra-Species Hit: Count: 1 Min: 34 Max: 263 Len: 230 Subject: NC_003454_FN0990_FN0991|Phosphoribosylformylglycinamidine synthase:CDP-diacylglycerol--serine O-phosphatidyltransferase|NEGATIVE:POSITIVE|[1645519, 1645949]|431 HSP 1 e-value: 1.0E-49 bit: 198.0 Len: 100 Query Start:164 Query End:263 Subject Strand: POSITIVE Subject Start: 164 Subject End: 263 ...................................................................................................................................................................gttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttcc........................................................................................................................................................................ ...................................................................................................................................................................gttaagcatacaaaaaatatatttccatattaaaaaagaaacacagtaaaacatttttcgtagctggttaatttatggttaaccgtagggacacccttcc........................................................................................................................................................................ HSP 2 e-value: 9.0E-35 bit: 149.0 Len: 75 Query Start:34 Query End:108 Subject Strand: POSITIVE Subject Start: 34 Subject End: 108 .................................tctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaa................................................................................................................................................................................................................................................................................................................................... .................................tctgagcaaaacactcagattataatttacaattacaactgttcgcaactatcccgtagaaaacagctaccttaa................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUGAGCAAAACACUCAGAUUAUAAUUUACAAUUACAACUGUUCGCAACUAUCCCGUAGAAAACAGCUACCUUAAAUUUUAUUUUAUUUAAAUUUUUUUUAGUUAUUAAUUUCACUGUUUCCUCCUUUUUGUUAAGCAUACAAAAAAUAUAUUUCCAUAUUAAAAAAGAAACACAGUAAAACAUUUUUCGUAGCUGGUUAAUUUAUGGUUAACCGUAGGGACACCCUUCC ((((((.......))))))................................((((........(((((((...((((....(((((((....(((((((((((....(((......)))......(((((((.......)))))))............))))))))))).....))))))).))))...))))))).......(((((....)))))))))......... (-34.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGAAGGGUGUCCCUACGGUUAACCAUAAAUUAACCAGCUACGAAAAAUGUUUUACUGUGUUUCUUUUUUAAUAUGGAAAUAUAUUUUUUGUAUGCUUAACAAAAAGGAGGAAACAGUGAAAUUAAUAACUAAAAAAAAUUUAAAUAAAAUAAAAUUUAAGGUAGCUGUUUUCUACGGGAUAGUUGCGAACAGUUGUAAUUGUAAAUUAUAAUCUGAGUGUUUUGCUCAGA ...((((...))))..((((((.......))))))...(((((.....((((((((((.((((((((((.(((..((((.....))))..))).........))))))))))))))))))))..((((((.........((((((........)))))).((((((((((......))))))))))....))))))..)))))........(((((((.....))))))) (-52.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 813 60 11 184 135 NC_003454:FN0990|5end_Phosphoribosylformylglycinamidine_synthase_1645428..1645658_NEGATIVE 244 127 81 136 90 NC_003454:FN0350|5end_hypothetical_protein_982372..982602_POSITIVE 235 230 184 102 59 NC_003454:FN1035|5end_Iron-sulfur_flavoprotein_1688280..1688510_POSITIVE 227 127 82 103 62 NC_003454:FN0664|5end_2-nitropropane_dioxygenase_1319758..1319988_POSITIVE 224 140 91 185 130 NC_003454:FN1896|5end_ABC_transporter_integral_membrane_protein_383909..384139_NEGATIVE 223 126 87 102 63 NC_003454:FN0417|5end_Type_III_restriction-modification_system_restriction_subunit_1055887..1056117_POSITIVE 223 128 82 59 6 NC_003454:FN0114|5end_GrpE_protein_747623..747853_POSITIVE 222 217 176 73 34 NC_003454:FN1867|5end_Zn-dependent_alcohol_dehydrogenase_and_related_dehydrogenase_359597..359827_NEGATIVE 213 229 195 104 69 NC_003454:FN1930|5end_Phosphatidylglycerophosphatase_A_430259..430489_NEGATIVE 211 160 114 107 58 NC_003454:FN1117|5end_LSU_ribosomal_protein_L21P_1760242..1760472_POSITIVE 210 104 64 119 76 NC_003454:FN0920|5end_Protease_HTPX_1575547..1575777_NEGATIVE 207 228 182 103 60 NC_003454:FN1677|5end_Transposase_174224..174454_NEGATIVE 206 136 107 138 110 NC_003454:FN1130|5end_Tetraacyldisaccharide_4'-kinase_1777840..1778070_POSITIVE 206 118 72 203 155 NC_003454:FN0587|5end_Hypothetical_membrane-spanning_protein_1242139..1242369_NEGATIVE 205 228 193 137 108 NC_003454:FN1269|5end_Hypothetical_lipoprotein_1926242..1926472_NEGATIVE 205 206 161 64 11 NC_003454:FN0879|5end_ABC_transporter_integral_membrane_protein_1540946..1541176_NEGATIVE 203 134 102 113 78 NC_003454:FN0013|5end_Transporter_652218..652448_POSITIVE 201 133 100 111 73 NC_003454:FN1376|5end_oxaloacetate_decarboxylase_2023879..2024109_POSITIVE 201 139 94 123 79 NC_003454:FN0207|5end_(R)-2-hydroxyglutaryl-CoA_dehydratase_alpha-subunit_833618..833848_POSITIVE 201 229 182 102 59 NC_003454:FN1733|5end_ATP_synthase_subunit_D_232871..233101_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 235 230 184 87 44 NC_003454:FN1034|3end_Transcriptional_regulator,_TetR_family_1688345..1688495_POSITIVE 227 127 82 62 21 NC_003454:FN0663|3end_hypothetical_protein_1319797..1319947_POSITIVE 224 140 91 129 74 NC_003454:FN1897|3end_ABC_transporter_integral_membrane_protein_383965..384115_NEGATIVE 223 126 87 92 53 NC_003454:FN0416|3end_Type_III_restriction-modification_system_methylation_subunit_1055957..1056107_POSITIVE 222 217 176 55 16 NC_003454:FN1868|3end_Hypothetical_cytosolic_protein_359615..359765_NEGATIVE 221 128 92 48 8 NC_003454:FN0113|3end_Heat-inducible_transcription_repressor_hrcA_747692..747842_POSITIVE 213 229 195 104 69 NC_003454:FN1931|3end_Protease_430259..430409_NEGATIVE 212 127 82 82 30 NC_003454:FN1342|3end_Holo-[acyl-carrier_protein]_synthase_1996091..1996241_NEGATIVE 210 104 64 113 70 NC_003454:FN0921|3end_hypothetical_protein_1575553..1575703_NEGATIVE 207 107 68 124 79 NC_003454:FN1476|3end_N-acetylmannosamine-6-phosphate_2-epimerase_2159426..2159576_POSITIVE 206 136 107 83 55 NC_003454:FN1129|3end_Chromosome_partition_protein_smc_1777865..1778015_POSITIVE 205 228 193 53 24 NC_003454:FN1270|3end_Hypothetical_cytosolic_protein_1926326..1926476_NEGATIVE 201 139 94 60 16 NC_003454:FN0206|3end_Activator_of_(R)-2-hydroxyglutaryl-CoA_dehydratase_833635..833785_POSITIVE 201 229 182 91 48 NC_003454:FN1734|3end_V-type_ATP_synthase_subunit_B_232882..233032_NEGATIVE 200 134 92 79 42 NC_003454:FN1319|3end_DNA_primase_1972386..1972536_NEGATIVE 197 135 93 101 56 NC_003454:FN1206|3end_Hemolysin_1865213..1865363_NEGATIVE 195 137 114 86 64 NC_003454:FN1629|3end_50S_ribosomal_protein_L6_133975..134125_NEGATIVE 194 155 111 71 29 NC_003454:FN2098|3end_MRP-family_nucleotide-binding_protein_607373..607523_NEGATIVE 193 130 98 88 58 NC_003454:FN1635|3end_LSU_ribosomal_protein_L14P_136204..136354_NEGATIVE 191 130 110 105 80 NC_003454:FN0583|3end_hypothetical_protein_1239518..1239668_POSITIVE