Origin IGS: atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aataaataaaataatagctgttacaaattagtgacagctatatttataactaaaggggaggagttatgaataagagggaaagtatcattgcactttatcaatatattgctgaggtaataaaaagtttaaagactgagaaaaaagatatttataatgaggagtggtattattttttagaaagtcttccaaaacattcaggaattacattcaactat Mask Tandem Repeat Region ================================================ atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Find is-nt database================================================ Query_seq: FN0949:FN0950|FN0949:FN0950:DNA helicase:Precorrin-6X reductase:<-<-:1606850..1607064 215 atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0949:FN0950|FN0949:FN0950:DNA helicase:Precorrin-6X reductase:<-<-:1606850..1607064 215 atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0949:FN0950|FN0949:FN0950:DNA helicase:Precorrin-6X reductase:<-<-:1606850..1607064 215 atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 18 End: 128 ................. M N V L E D F L K N N T T P H Y K Y L F S Q S L N F L L P Q Q Y I D K V Q ....................................................................................... Protein_Len: 50 Strand: - Start: 1 End: 150 Y N F T I G S H K P L S E L F Y Y W E E N Y I D K K E T K L S K I V E A I Y Q Y L A I I S E R K N M ................................................................. atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0949:FN0950|FN0949:FN0950:DNA helicase:Precorrin-6X reductase:<-<-:1606850..1607064 215 atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Intra-Species Hit: Count: 1 Min: 1 Max: 215 Len: 215 Subject: NC_003454_FN0949_FN0950|DNA helicase:Precorrin-6X reductase|NEGATIVE:NEGATIVE|[1606850,1607064]|215 HSP 1 e-value: 1.0E-118 bit: 426.0 Len: 215 Query Start:1 Query End:215 Subject Strand: POSITIVE Subject Start: 1 Subject End: 215 atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt atagttgaatgtaattcctgaatgttttggaagactttctaaaaaataataccactcctcattataaatatcttttttctcagtctttaaactttttattacctcagcaatatattgataaagtgcaatgatactttccctcttattcataactcctcccctttagttataaatatagctgtcactaatttgtaacagctattattttatttatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGUUGAAUGUAAUUCCUGAAUGUUUUGGAAGACUUUCUAAAAAAUAAUACCACUCCUCAUUAUAAAUAUCUUUUUUCUCAGUCUUUAAACUUUUUAUUACCUCAGCAAUAUAUUGAUAAAGUGCAAUGAUACUUUCCCUCUUAUUCAUAACUCCUCCCCUUUAGUUAUAAAUAUAGCUGUCACUAAUUUGUAACAGCUAUUAUUUUAUUUAUU ...(((((..(((((....(((.((((..(((((((....(((((...........................)))))....))))))))))).))).))))).)))))........(((((((((..................((((.((((((..........)))))).))))(((((((.((......)).))))))).))))))))).... (-31.03) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAAAUAAAAUAAUAGCUGUUACAAAUUAGUGACAGCUAUAUUUAUAACUAAAGGGGAGGAGUUAUGAAUAAGAGGGAAAGUAUCAUUGCACUUUAUCAAUAUAUUGCUGAGGUAAUAAAAAGUUUAAAGACUGAGAAAAAAGAUAUUUAUAAUGAGGAGUGGUAUUAUUUUUUAGAAAGUCUUCCAAAACAUUCAGGAAUUACAUUCAACUAU .............(((((((((((......)))))))))))((((((((((..........))))))))))..((((((.(((((((((.(.(.((((.((((((((((....))))))...((((....))))...........)))).)))).).).))))))))).)))))).(((.((.((((..........))))..)).)))...... (-42.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 765 60 11 151 102 NC_003454:FN0949|5end_DNA_helicase_1606759..1606989_NEGATIVE 275 187 143 160 102 NC_003454:FN2092|5end_Integral_membrane_protein_603111..603341_NEGATIVE 254 170 129 119 62 NC_003454:FN1787|5end_Tetratricopeptide_repeat_family_protein_267762..267992_POSITIVE 241 75 32 117 72 NC_003454:FN0285|5end_RNA_binding_protein_914171..914401_NEGATIVE 236 186 143 131 84 NC_003454:FN0788|5end_hypothetical_protein_1444359..1444589_NEGATIVE 236 187 146 133 87 NC_003454:FN0772|5end_Flavodoxin_1428694..1428924_POSITIVE 236 187 147 48 9 NC_003454:FN1594|5end_Nitrogen_fixation_protein_RNFG_104893..105123_NEGATIVE 234 165 122 218 174 NC_003454:FN1426|5end_Serine_protease_2095462..2095692_NEGATIVE 234 150 102 110 66 NC_003454:FN0298|5end_Histidyl-tRNA_synthetase_931133..931363_POSITIVE 232 163 129 224 191 NC_003454:FN1261|5end_Two-component_response_regulator_1917871..1918101_NEGATIVE 232 147 101 147 87 NC_003454:FN0247|5end_Hypothetical_cytosolic_protein_870707..870937_POSITIVE 231 169 142 108 78 NC_003454:FN1829|5end_hypothetical_protein_318556..318786_NEGATIVE 229 160 125 89 45 NC_003454:FN1008|5end_hypothetical_protein_1663067..1663297_NEGATIVE 228 169 124 48 8 NC_003454:FN1092|5end_hypothetical_protein_1737267..1737497_POSITIVE 222 132 92 61 21 NC_003454:FN0958|5end_hypothetical_protein_1614297..1614527_NEGATIVE 220 183 141 73 7 NC_003454:FN1466|5end_hypothetical_protein_2148862..2149092_POSITIVE 218 110 66 102 58 NC_003454:FN0663|5end_hypothetical_protein_1319286..1319516_POSITIVE 217 150 108 111 73 NC_003454:FN0362|5end_ATP_synthase_B_chain,_sodium_ion_specific_993393..993623_NEGATIVE 215 173 144 85 57 NC_003454:FN0864|5end_hypothetical_protein_1522900..1523130_NEGATIVE 214 200 155 65 18 NC_003454:FN1070|5end_glucose-inhibited_division_protein_A_1717121..1717351_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 254 170 129 98 41 NC_003454:FN1786|3end_ADP-heptose_synthase_267821..267971_POSITIVE 249 170 129 98 59 NC_003454:FN1294|3end_Ribosomal-protein-alanine_acetyltransferase_1952517..1952667_NEGATIVE 241 75 32 105 60 NC_003454:FN0286|3end_hypothetical_protein_914183..914333_NEGATIVE 236 186 143 54 7 NC_003454:FN0789|3end_Transporter_1444436..1444586_NEGATIVE 236 187 146 136 90 NC_003454:FN0771|3end_Oxygen-independent_coproporphyrinogen_III_oxidase_1428777..1428927_POSITIVE 236 187 147 58 19 NC_003454:FN1595|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_B_104883..105033_NEGATIVE 235 99 56 144 98 NC_003454:FN1417|3end_L-fuculose_phosphate_aldolase_2079020..2079170_POSITIVE 234 150 102 98 54 NC_003454:FN0297|3end_ATPase_associated_with_chromosome_architecture/replication_931201..931351_POSITIVE 231 169 142 93 63 NC_003454:FN1830|3end_DNA_polymerase_III_subunits_gamma_and_tau_318571..318721_NEGATIVE 227 171 139 41 15 NC_003454:FN1091|3end_Sigma_factor_sigB_regulation_protein_rsbU_1737338..1737488_POSITIVE 226 209 166 88 48 NC_003454:FN1141|3end_Formate_transporter_1790493..1790643_NEGATIVE 222 132 92 46 6 NC_003454:FN0959|3end_Precorrin-2_C20-methyltransferase_1614312..1614462_NEGATIVE 220 183 141 72 6 NC_003454:FN1465|3end_Permease_2148941..2149091_POSITIVE 217 150 108 70 32 NC_003454:FN0363|3end_ATP_synthase_C_chain,_sodium_ion_specific_993434..993584_NEGATIVE 213 115 75 142 108 NC_003454:FN1996|3end_Transporter_499742..499892_POSITIVE 212 104 74 89 60 NC_003454:FN1603|3end_2',3'-cyclic_nucleotide_3'-phosphodiesterase_111610..111760_NEGATIVE 210 166 122 83 21 NC_003454:FN1914|3end_Anti-sigma_F_factor_antagonist_411643..411793_NEGATIVE 207 189 141 109 57 NC_003454:FN0294|3end_Transketolase_subunit_A_928041..928191_POSITIVE 204 203 171 84 44 NC_003454:FN1927|3end_DEGV_protein_425415..425565_NEGATIVE 202 166 122 44 5 NC_003454:FN1822|3end_Flavodoxin_311737..311887_POSITIVE