Origin IGS: tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttcgttattgagtagatttcttaacaataaaaaatctacattcataactcacttatttttcaatatcatttaagattggaggtttttatgtatataataaatata Mask Tandem Repeat Region ================================================ tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Find is-nt database================================================ Query_seq: FN0921:FN0922|FN0921:FN0922:hypothetical protein:Homoserine kinase:<-<-:1576573..1576677 105 tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0921:FN0922|FN0921:FN0922:hypothetical protein:Homoserine kinase:<-<-:1576573..1576677 105 tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0921:FN0922|FN0921:FN0922:hypothetical protein:Homoserine kinase:<-<-:1576573..1576677 105 tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Predict ORF larger than 30AA ================================================ tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 8 End: 37 .......TTTAAGATTGGAGGTTTTTATGTATATAAT.................................................................... PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 37 End: 66 ....................................ATTCATAACTCACTTATTTTTCAATATCAT....................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0921:FN0922|FN0921:FN0922:hypothetical protein:Homoserine kinase:<-<-:1576573..1576677 105 tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Intra-Species Hit: Count: 1 Min: 1 Max: 105 Len: 105 Subject: NC_003454_FN0921_FN0922|hypothetical protein:Homoserine kinase|NEGATIVE:NEGATIVE|[1576573,1576677]|105 HSP 1 e-value: 3.0E-53 bit: 208.0 Len: 105 Query Start:1 Query End:105 Subject Strand: POSITIVE Subject Start: 1 Subject End: 105 tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa tatatttattatatacataaaaacctccaatcttaaatgatattgaaaaataagtgagttatgaatgtagattttttattgttaagaaatctactcaataacgaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAUUUAUUAUAUACAUAAAAACCUCCAAUCUUAAAUGAUAUUGAAAAAUAAGUGAGUUAUGAAUGUAGAUUUUUUAUUGUUAAGAAAUCUACUCAAUAACGAA ..((((((((((((.(((..................))))))).....)))))))).(((((....((((((((((((....))))))))))))...)))))... (-17.57) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCGUUAUUGAGUAGAUUUCUUAACAAUAAAAAAUCUACAUUCAUAACUCACUUAUUUUUCAAUAUCAUUUAAGAUUGGAGGUUUUUAUGUAUAUAAUAAAUAUA ...(((((.((((((((((.(((....))).)))))))).)).))))).....(((((....(((((((..(((((.....))))).))).))))...))))).. (-13.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 713 70 21 161 112 NC_003454:FN0921|5end_hypothetical_protein_1576482..1576712_NEGATIVE 587 97 51 192 146 NC_003454:FN2080|5end_ABC_transporter_integral_membrane_protein_591957..592187_NEGATIVE 414 97 51 224 182 NC_003454:FN1347|5end_Hypothetical_cytosolic_protein_2000300..2000530_NEGATIVE 383 97 51 196 154 NC_003454:FN0423|5end_Dihydroorotate_dehydrogenase_electron_transfer_subunit_1066328..1066558_POSITIVE 382 97 51 227 185 NC_003454:FN1350|5end_Integral_membrane_protein_2002796..2003026_NEGATIVE 314 97 53 84 46 NC_003454:FN0171|5end_Hypothetical_cytosolic_protein_796548..796778_POSITIVE 304 64 24 130 85 NC_003454:FN0054|5end_tyrosyl-tRNA_synthetase_690836..691066_POSITIVE 267 62 24 159 115 NC_003454:FN1835|5end_hypothetical_protein_323680..323910_NEGATIVE 230 32 8 105 75 NC_003454:FN0836|5end_hypothetical_protein_1498802..1499032_POSITIVE 217 48 16 119 89 NC_003454:FN1235|5end_Ankyrin_repeat_proteins_1890915..1891145_POSITIVE 216 90 42 80 38 NC_003454:FN0797|5end_hypothetical_protein_1455799..1456029_NEGATIVE 212 75 47 176 148 NC_003454:FN0988|5end_phosphoribosylaminoimidazole-succinocarboxamide_synthase_1640968..1641198_NEGATIVE 204 78 31 161 108 NC_003454:FN1924|5end_Arsenical_pump_membrane_protein_422993..423223_NEGATIVE 204 59 15 76 31 NC_003454:FN1746|5end_Cystathionine_beta-lyase_244341..244571_POSITIVE 198 48 6 124 76 NC_003454:FN0898|5end_Spore_photoproduct_1556576..1556806_POSITIVE 194 61 23 147 106 NC_003454:FN0906|5end_Glycerol-3-phosphate_dehydrogenase_[NAD(P)+]_1565131..1565361_NEGATIVE 192 61 24 159 102 NC_003454:FN0070|5end_Glycyl-tRNA_synthetase_beta_chain_708902..709132_POSITIVE 191 62 28 178 143 NC_003454:FN1459|5end_Phospho-N-acetylmuramoyl-pentapeptide-_transferase_2141360..2141590_NEGATIVE 188 100 55 198 144 NC_003454:FN1606|5end_3-deoxy-D-manno-octulosonic-acid_transferase_117208..117438_NEGATIVE 186 57 16 122 89 NC_003454:FN1310|5end_TonB_protein_1965876..1966106_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 587 97 51 74 28 NC_003454:FN2081|3end_ABC_transporter_substrate-binding_protein_592075..592225_NEGATIVE 414 97 51 54 12 NC_003454:FN1348|3end_ABC_transporter_ATP-binding_protein_2000470..2000620_NEGATIVE 383 97 51 71 29 NC_003454:FN0422|3end_carbamoyl-phosphate_synthase_large_subunit_1066283..1066433_POSITIVE 382 97 51 73 31 NC_003454:FN1351|3end_15_kDa_lipoprotein_precursor_2002950..2003100_NEGATIVE 322 95 53 62 26 NC_003454:FN0989|3end_phosphoribosylaminoimidazole_carboxylase_catalytic_subunit_1641194..1641344_NEGATIVE 314 97 53 69 31 NC_003454:FN0170|3end_GTP-binding_protein_796613..796763_POSITIVE 255 92 52 71 17 NC_003454:FN2096|3end_hypothetical_protein_606267..606417_NEGATIVE 248 92 53 71 18 NC_003454:FN0391|3end_Hydrolase_(HAD_superfamily)_1029496..1029646_POSITIVE 244 92 53 57 13 NC_003454:FN0069|3end_glycyl-tRNA_synthetase_alpha_subunit_708761..708911_POSITIVE 240 99 52 84 21 NC_003454:FN2005|3end_Cobalt_transport_ATP-binding_protein_cbiO_505976..506126_POSITIVE 230 32 8 88 58 NC_003454:FN0835|3end_hypothetical_protein_1498865..1499015_POSITIVE 216 89 41 113 72 NC_003454:FN1964|3end_O-linked_GLCNAC_transferase_461646..461796_NEGATIVE 206 99 52 69 6 NC_003454:FN1218|3end_hypothetical_protein_1877812..1877962_NEGATIVE 204 78 31 93 40 NC_003454:FN1925|3end_Arsenical_pump_membrane_protein_423061..423211_NEGATIVE 198 48 6 49 1 NC_003454:FN0897|3end_Hypothetical_cytosolic_protein_1556581..1556731_POSITIVE 197 99 53 70 7 NC_003454:FN1811|3end_Manganese_transport_system_ATP-binding_protein_mntA_292161..292311_NEGATIVE 188 78 37 136 82 NC_003454:FN1319|3end_DNA_primase_1972386..1972536_NEGATIVE 186 57 16 50 17 NC_003454:FN1311|3end_Biopolymer_transport_exbD_protein_1965948..1966098_NEGATIVE 182 33 15 92 74 NC_003454:FN0874|3end_Phosphohydrolase_(MUTT/NUDIX_family_protein)_1534372..1534522_NEGATIVE 181 34 4 116 85 NC_003454:FN1886|3end_hypothetical_protein_371754..371904_POSITIVE