Origin IGS: aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gctatattagaattaataaaataaatatgaactgtacttctaattctgtaattaacatctttggtacagttcatttaatatcttttatattttattaatgtttaattt Mask Tandem Repeat Region ================================================ aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Find is-nt database================================================ Query_seq: FN0886:FN0887|FN0886:FN0887:Hemin receptor:Oligoendopeptidase F:-><-:1547891..1547998 108 aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0886:FN0887|FN0886:FN0887:Hemin receptor:Oligoendopeptidase F:-><-:1547891..1547998 108 aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0886:FN0887|FN0886:FN0887:Hemin receptor:Oligoendopeptidase F:-><-:1547891..1547998 108 aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 14 End: 103 ............. Y F I F S I L H V T G F I N I V S N S T C N M N I K N I R M ..... aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0886:FN0887|FN0886:FN0887:Hemin receptor:Oligoendopeptidase F:-><-:1547891..1547998 108 aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Intra-Species Hit: Count: 1 Min: 1 Max: 108 Len: 108 Subject: NC_003454_FN0886_FN0887|Hemin receptor:Oligoendopeptidase F|POSITIVE:NEGATIVE|[1547891,1547998]|108 HSP 1 e-value: 4.0E-55 bit: 214.0 Len: 108 Query Start:1 Query End:108 Subject Strand: POSITIVE Subject Start: 1 Subject End: 108 aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc aaattaaacattaataaaatataaaagatattaaatgaactgtaccaaagatgttaattacagaattagaagtacagttcatatttattttattaattctaatatagc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUUAAACAUUAAUAAAAUAUAAAAGAUAUUAAAUGAACUGUACCAAAGAUGUUAAUUACAGAAUUAGAAGUACAGUUCAUAUUUAUUUUAUUAAUUCUAAUAUAGC ..((((...((((((((((((.............(((((((((((......(((.....))).........)))))))))))...))))))))))))..))))..... (-19.15) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUAUAUUAGAAUUAAUAAAAUAAAUAUGAACUGUACUUCUAAUUCUGUAAUUAACAUCUUUGGUACAGUUCAUUUAAUAUCUUUUAUAUUUUAUUAAUGUUUAAUUU .....((((((((((((((((((...((((((((((((........(((.....))).....)))))))))))).............)))))))))))).)))))).. (-22.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 194 100 65 104 65 NC_003454:FN0817|5end_ABC_transporter_ATP-binding_protein_1476448..1476678_POSITIVE 190 86 43 163 117 NC_003454:FN1337|5end_hypothetical_protein_1991481..1991711_NEGATIVE 177 79 41 203 168 NC_003454:FN1022|5end_Calcium-transporting_ATPase_1674833..1675063_POSITIVE 177 80 36 228 179 NC_003454:FN0363|5end_ATP_synthase_C_chain,_sodium_ion_specific_993703..993933_NEGATIVE 167 65 25 68 27 NC_003454:FN0467|5end_murein_hydrolase_exporter_1112864..1113094_POSITIVE 164 79 35 72 28 NC_003454:FN0839|5end_hypothetical_protein_1501426..1501656_NEGATIVE 163 78 36 107 59 NC_003454:FN1528|5end_hypothetical_protein_42944..43174_NEGATIVE 162 99 66 110 73 NC_003454:FN1404|5end_Imidazolonepropionase_2063066..2063296_POSITIVE 161 98 62 59 27 NC_003454:FN0044|5end_Shikimate_5-dehydrogenase_682701..682931_POSITIVE 161 81 41 184 134 NC_003454:FN1603|5end_2',3'-cyclic_nucleotide_3'-phosphodiesterase_113661..113891_NEGATIVE 159 100 67 106 79 NC_003454:FN1307|5end_CDP-diacylglycerol--glycerol-3-phosphate_3-phosphatidyltransferase_1963377..1963607_POSITIVE 156 74 47 48 21 NC_003454:FN1047|5end_Hypothetical_membrane-spanning_protein_1697175..1697405_POSITIVE 155 64 29 175 138 NC_003454:FN0192|5end_Dipeptide-binding_protein_817342..817572_POSITIVE 149 68 23 81 34 NC_003454:FN0350|5end_hypothetical_protein_982372..982602_POSITIVE 148 77 42 71 36 NC_003454:FN0738|5end_Hypothetical_exported_24-amino_acid_repeat_protein_1395846..1396076_POSITIVE 147 43 13 46 9 NC_003454:FN0141|5end_hypothetical_protein_773265..773495_NEGATIVE 145 46 33 217 204 NC_003454:FN0234|5end_hypothetical_protein_859499..859729_NEGATIVE 142 48 1 100 55 NC_003454:FN0481|5end_hypothetical_protein_1127777..1128007_POSITIVE 141 93 60 96 69 NC_003454:FN1923|5end_Adenine-specific_methyltransferase_421097..421327_NEGATIVE 140 81 49 212 169 NC_003454:FN0565|5end_hypothetical_protein_1217464..1217694_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 261 80 34 74 27 NC_003454:FN1022|3end_Calcium-transporting_ATPase_1677501..1677651_POSITIVE 247 47 8 52 14 NC_003454:FN1143|3end_glucosamine-6-phosphate_deaminase_1793365..1793515_POSITIVE 237 80 32 63 14 NC_003454:FN1023|3end_5-Nitroimidazole_antibiotic_resistance_protein_1677560..1677710_NEGATIVE 220 79 32 80 32 NC_003454:FN1499|3end_Cell_surface_protein_5166..5316_POSITIVE 207 80 33 79 32 NC_003454:FN1498|3end_Integral_membrane_protein_3542..3692_POSITIVE 190 86 43 90 44 NC_003454:FN1338|3end_hypothetical_protein_1991554..1991704_NEGATIVE 172 52 23 65 31 NC_003454:FN0436|3end_Urea_carboxylase_1077851..1078001_NEGATIVE 169 80 35 54 9 NC_003454:FN0886|3end_Hemin_receptor_1547830..1547980_POSITIVE 167 79 33 80 32 NC_003454:FN1719|3end_hypothetical_protein_218747..218897_POSITIVE 167 79 33 77 29 NC_003454:FN1546|3end_translation_elongation_factor_EF-G_59529..59679_NEGATIVE 163 78 36 58 10 NC_003454:FN1529|3end_hypothetical_protein_42993..43143_NEGATIVE 162 99 66 97 60 NC_003454:FN1403|3end_Histidine_permease_2063133..2063283_POSITIVE 161 98 62 96 64 NC_003454:FN0043|3end_Hypothetical_exported_24-amino_acid_repeat_protein_682818..682968_POSITIVE 159 100 67 103 76 NC_003454:FN1306|3end_Methyltransferase_1963454..1963604_POSITIVE 151 77 39 82 48 NC_003454:FN0550|3end_Mannose-1-phosphate_guanylyl_transferase_(GDP)_1203166..1203316_POSITIVE 150 41 22 148 129 NC_003454:FN0935|3end_hypothetical_protein_1587558..1587708_NEGATIVE 149 49 11 57 30 NC_003454:FN0981|3end_phosphoribosylamine--glycine_ligase_1632840..1632990_NEGATIVE 147 43 13 108 71 NC_003454:FN0141|3end_hypothetical_protein_773203..773353_NEGATIVE 146 80 37 83 40 NC_003454:FN1463|3end_pyridoxine_biosynthesis_protein_2145924..2146074_POSITIVE 146 76 36 46 1 NC_003454:FN1019|3end_3-hydroxybutyryl-CoA_dehydrogenase_1672945..1673095_NEGATIVE