Origin IGS: ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aactaataaaacctttttcattttgtcccctcccctttctttttaaaataataggattaaactccaagtatatattattctataataattataacatattttatcaaaatagtttagaa Mask Tandem Repeat Region ================================================ ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Find is-nt database================================================ Query_seq: FN0820:FN0821|FN0820:FN0821:Mercuric reductase:hypothetical protein:->->:1480880..1480998 119 ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0820:FN0821|FN0820:FN0821:Mercuric reductase:hypothetical protein:->->:1480880..1480998 119 ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0820:FN0821|FN0820:FN0821:Mercuric reductase:hypothetical protein:->->:1480880..1480998 119 ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: - Start: 1 End: 114 E L S N Q Y F I N Y N N Y F L I Y K S N L G I I K F L F P L P C F S F P K M ..... ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 3 End: 32 ..CTAAACTATTTTGATAAAATATGTTATAAT....................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0820:FN0821|FN0820:FN0821:Mercuric reductase:hypothetical protein:->->:1480880..1480998 119 ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Intra-Species Hit: Count: 1 Min: 1 Max: 119 Len: 119 Subject: NC_003454_FN0820_FN0821|Mercuric reductase:hypothetical protein|POSITIVE:POSITIVE|[1480880,1480998]|119 HSP 1 e-value: 1.0E-61 bit: 236.0 Len: 119 Query Start:1 Query End:119 Subject Strand: POSITIVE Subject Start: 1 Subject End: 119 ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt ttctaaactattttgataaaatatgttataattattatagaataatatatacttggagtttaatcctattattttaaaaagaaaggggaggggacaaaatgaaaaaggttttattagtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUAAACUAUUUUGAUAAAAUAUGUUAUAAUUAUUAUAGAAUAAUAUAUACUUGGAGUUUAAUCCUAUUAUUUUAAAAAGAAAGGGGAGGGGACAAAAUGAAAAAGGUUUUAUUAGUU ....(((((.((((.((...(((((((((..(......)..))))))))).......((((..((((....((((.....)))).))))..))))...)).)))).)))))........ (-16.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACUAAUAAAACCUUUUUCAUUUUGUCCCCUCCCCUUUCUUUUUAAAAUAAUAGGAUUAAACUCCAAGUAUAUAUUAUUCUAUAAUAAUUAUAACAUAUUUUAUCAAAAUAGUUUAGAA ...................((((((..................(((((((...(((......)))...((((.((((((....))))))))))...))))))).))))))......... ( -6.81) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 227 108 86 203 183 NC_003454:FN0897|5end_Hypothetical_cytosolic_protein_1555833..1556063_POSITIVE 202 109 70 204 165 NC_003454:FN1435|5end_Export_ABC_transporter_2105034..2105264_POSITIVE 202 94 57 162 124 NC_003454:FN1302|5end_hypothetical_protein_1959369..1959599_POSITIVE 197 60 11 161 106 NC_003454:FN0773|5end_hypothetical_protein_1429274..1429504_POSITIVE 196 98 53 59 14 NC_003454:FN1440|5end_1-phosphofructokinase_2107238..2107468_POSITIVE 191 108 69 208 163 NC_003454:FN1262|5end_Integral_membrane_protein_1918043..1918273_POSITIVE 183 53 17 147 112 NC_003454:FN2053|5end_Serine/threonine_sodium_symporter_562415..562645_NEGATIVE 180 110 70 223 184 NC_003454:FN2083|5end_hypothetical_protein_595642..595872_NEGATIVE 179 97 80 210 190 NC_003454:FN1460|5end_Hypothetical_cytosolic_protein_2141429..2141659_POSITIVE 179 106 64 50 2 NC_003454:FN1360|5end_Dipeptide_transport_system_permease_protein_dppB_2012134..2012364_POSITIVE 169 86 43 131 80 NC_003454:FN2109|5end_UDP-glucose_4-epimerase_620848..621078_POSITIVE 161 97 76 148 128 NC_003454:FN0467|5end_murein_hydrolase_exporter_1112864..1113094_POSITIVE 160 57 12 192 145 NC_003454:FN1656|5end_SSU_ribosomal_protein_S18P_152760..152990_NEGATIVE 159 93 54 185 141 NC_003454:FN1337|5end_hypothetical_protein_1991481..1991711_NEGATIVE 159 66 27 151 108 NC_003454:FN1078|5end_Hypothetical_exported_24-amino_acid_repeat_protein_1724153..1724383_POSITIVE 157 94 62 217 185 NC_003454:FN1225|5end_UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--meso-_lanthionine_ligase_1884823..1885053_NEGATIVE 156 75 36 213 164 NC_003454:FN0930|5end_Glycerol-3-phosphate_cytidylyltransferase_1583964..1584194_NEGATIVE 153 100 52 142 100 NC_003454:FN0073|5end_Dihydropteroate_synthase_712520..712750_POSITIVE 152 38 1 221 188 NC_003454:FN0003|5end_Alpha-hemolysin_642892..643122_POSITIVE 151 110 80 208 180 NC_003454:FN0691|5end_hypothetical_protein_1346372..1346602_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 206 100 54 66 19 NC_003454:FN1426|3end_Serine_protease_2092577..2092727_NEGATIVE 202 94 57 81 43 NC_003454:FN1301|3end_ABC_transporter_ATP-binding_protein_1959368..1959518_POSITIVE 198 109 66 65 14 NC_003454:FN1340|3end_Glutamyl-tRNA_synthetase_1993353..1993503_NEGATIVE 197 60 11 90 35 NC_003454:FN0772|3end_Flavodoxin_1429283..1429433_POSITIVE 185 89 42 78 39 NC_003454:FN0500|3end_hypothetical_protein_1149592..1149742_POSITIVE 169 86 43 131 80 NC_003454:FN2108|3end_galactose-1-phosphate_uridylyltransferase_620928..621078_POSITIVE 165 109 72 46 1 NC_003454:FN0528|3end_Cold_shock_protein_1184746..1184896_POSITIVE 164 109 75 39 5 NC_003454:FN0996|3end_Hypothetical_membrane-spanning_protein_1650673..1650823_NEGATIVE 164 98 55 61 16 NC_003454:FN1800|3end_Peptidyl-prolyl_cis-trans_isomerase_282534..282684_POSITIVE 161 97 76 66 46 NC_003454:FN0466|3end_Lysyl-tRNA_synthetase_1112862..1113012_POSITIVE 161 109 79 42 4 NC_003454:FN0371|3end_hypothetical_protein_1000840..1000990_POSITIVE 160 57 12 147 100 NC_003454:FN1657|3end_SSU_ribosomal_protein_S6P_152805..152955_NEGATIVE 159 93 54 112 68 NC_003454:FN1338|3end_hypothetical_protein_1991554..1991704_NEGATIVE 158 88 66 28 4 NC_003454:FN0072|3end_2-amino-4-hydroxy-6-_hydroxymethyldihydropteridine_pyrophosphokinase_712619..712769_POSITIVE 158 70 25 151 107 NC_003454:FN1919|3end_Methyltransferase_415133..415283_NEGATIVE 153 103 79 37 12 NC_003454:FN0736|3end_Methyltransferase_1394658..1394808_NEGATIVE 149 106 71 40 3 NC_003454:FN1359|3end_Dipeptide-binding_protein_2012204..2012354_POSITIVE 149 73 35 87 49 NC_003454:FN0937|3end_Gamma-polyglutamic_acid_synthetase_1589097..1589247_NEGATIVE 149 94 64 70 35 NC_003454:FN0262|3end_Formate_acetyltransferase_887576..887726_NEGATIVE 148 87 66 37 14 NC_003454:FN0498|3end_hypothetical_protein_1141538..1141688_NEGATIVE