Origin IGS: aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttcctccttaaaaaattaaaataattatttgtaaaaatcttttagattatgttagtacagcacttcagagtcagtaaattttttcacttcattagtatttattagattgtataatcaacatctaaaataaacttaaattttcacatctttaggaatgttatttttcttaagctcaagctaattctaatacataatcaatttactttttaatattaagacttattttttataactt Mask Tandem Repeat Region ================================================ aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Find is-nt database================================================ Query_seq: FN0815:FN0816|FN0815:FN0816:Propionate permease:dehydrogenase with MaoC-like domain:->->:1475874..1476110 237 aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0815:FN0816|FN0815:FN0816:Propionate permease:dehydrogenase with MaoC-like domain:->->:1475874..1476110 237 aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0815:FN0816|FN0815:FN0816:Propionate permease:dehydrogenase with MaoC-like domain:->->:1475874..1476110 237 aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Predict ORF larger than 30AA ================================================ aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0815:FN0816|FN0815:FN0816:Propionate permease:dehydrogenase with MaoC-like domain:->->:1475874..1476110 237 aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 237 Len: 237 Subject: NC_003454_FN0815_FN0816|Propionate permease:dehydrogenase with MaoC-like domain|POSITIVE:POSITIVE|[1475874,1476110]|237 HSP 1 e-value: 1.0E-131 bit: 470.0 Len: 237 Query Start:1 Query End:237 Subject Strand: POSITIVE Subject Start: 1 Subject End: 237 aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa aagttataaaaaataagtcttaatattaaaaagtaaattgattatgtattagaattagcttgagcttaagaaaaataacattcctaaagatgtgaaaatttaagtttattttagatgttgattatacaatctaataaatactaatgaagtgaaaaaatttactgactctgaagtgctgtactaacataatctaaaagatttttacaaataattattttaattttttaaggaggaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAGUUAUAAAAAAUAAGUCUUAAUAUUAAAAAGUAAAUUGAUUAUGUAUUAGAAUUAGCUUGAGCUUAAGAAAAAUAACAUUCCUAAAGAUGUGAAAAUUUAAGUUUAUUUUAGAUGUUGAUUAUACAAUCUAAUAAAUACUAAUGAAGUGAAAAAAUUUACUGACUCUGAAGUGCUGUACUAACAUAAUCUAAAAGAUUUUUACAAAUAAUUAUUUUAAUUUUUUAAGGAGGAAAA .................((((....(((((((((.....((((((((..(((...((((.((((((((((.......(((((......))))).....)))))))))).((((((.(((((((....))))................((((((....)))))))))))))))..))))..)))))))))))...(((((..(((....)))..))))).)))))))))..))))... (-35.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCCUCCUUAAAAAAUUAAAAUAAUUAUUUGUAAAAAUCUUUUAGAUUAUGUUAGUACAGCACUUCAGAGUCAGUAAAUUUUUUCACUUCAUUAGUAUUUAUUAGAUUGUAUAAUCAACAUCUAAAAUAAACUUAAAUUUUCACAUCUUUAGGAAUGUUAUUUUUCUUAAGCUCAAGCUAAUUCUAAUACAUAAUCAAUUUACUUUUUAAUAUUAAGACUUAUUUUUUAUAACUU ..............................(((((((((.......(((((((((((...(((......((((..............(((.....))).....((((((.((.......)))))))).....)))).............((((((((........)))))))).....)))....)))..))))))))......(((.........)))......)))))))))... (-25.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 219 116 75 203 159 NC_003454:FN1554|5end_Fusobacterium_outer_membrane_protein_family_73467..73697_NEGATIVE 213 198 163 191 152 NC_003454:FN1255|5end_NagD_protein_1911892..1912122_NEGATIVE 203 180 146 56 22 NC_003454:FN0711|5end_Phosphopantothenate--cysteine_ligase_1369569..1369799_POSITIVE 190 80 32 90 50 NC_003454:FN1647|5end_hypothetical_protein_141983..142213_POSITIVE 185 184 159 54 25 NC_003454:FN1595|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_B_105827..106057_NEGATIVE 181 93 61 160 119 NC_003454:FN0628|5end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing]_1288257..1288487_NEGATIVE 177 61 38 207 185 NC_003454:FN0274|5end_Transposase_900485..900715_NEGATIVE 177 188 160 92 59 NC_003454:FN1675|5end_Transposase_172478..172708_NEGATIVE 172 116 76 210 166 NC_003454:FN1347|5end_Hypothetical_cytosolic_protein_2000300..2000530_NEGATIVE 172 195 158 74 27 NC_003454:FN0182|5end_Sarcosine_oxidase_alpha_subunit_807066..807296_NEGATIVE 170 151 112 196 164 NC_003454:FN1397|5end_glutaminase_2051836..2052066_POSITIVE 167 108 68 146 107 NC_003454:FN1311|5end_Biopolymer_transport_exbD_protein_1966250..1966480_NEGATIVE 165 197 166 220 187 NC_003454:FN1329|5end_Methyltransferase_1983890..1984120_NEGATIVE 165 176 136 184 151 NC_003454:FN0553|5end_D-serine_dehydratase_1206060..1206290_NEGATIVE 164 110 75 107 78 NC_003454:FN0849|5end_8-amino-7-oxononanoate_synthase_1507767..1507997_POSITIVE 164 116 75 194 150 NC_003454:FN1808|5end_hypothetical_protein_289905..290135_NEGATIVE 163 180 137 106 60 NC_003454:FN1450|5end_Integral_membrane_protein_2131410..2131640_NEGATIVE 162 90 60 87 57 NC_003454:FN1509|5end_hypothetical_protein_14176..14406_NEGATIVE 161 120 71 72 21 NC_003454:FN0340|5end_Hypothetical_membrane-spanning_Protein_973948..974178_NEGATIVE 160 204 161 83 46 NC_003454:FN0399|5end_Dipeptide_transport_ATP-binding_protein_dppD_1037646..1037876_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 203 180 146 39 5 NC_003454:FN0710|3end_hypothetical_protein_1369632..1369782_POSITIVE 181 93 61 150 109 NC_003454:FN0629|3end_PTS_system,_IID_component_1288267..1288417_NEGATIVE 177 61 38 85 63 NC_003454:FN0275|3end_Transposase_900607..900757_NEGATIVE 175 60 12 41 7 NC_003454:FN1125|3end_LemA_protein_1769260..1769410_NEGATIVE 174 66 27 92 43 NC_003454:FN1732|3end_hypothetical_protein_231281..231431_NEGATIVE 172 195 158 62 15 NC_003454:FN0183|3end_Glycerol-3-phosphate_dehydrogenase_807078..807228_NEGATIVE 167 108 68 56 17 NC_003454:FN1312|3end_Biopolymer_transport_exbB_protein_1966340..1966490_NEGATIVE 165 176 136 150 117 NC_003454:FN0554|3end_D-serine_permease_1206094..1206244_NEGATIVE 165 184 161 28 1 NC_003454:FN1596|3end_Nitrogen_fixation_iron-sulphur_protein_RNFC_105853..106003_NEGATIVE 162 174 136 76 36 NC_003454:FN1442|3end_Transposase_2110155..2110305_NEGATIVE 162 90 60 32 2 NC_003454:FN0404|3end_hypothetical_protein_1042373..1042523_POSITIVE 162 90 60 32 2 NC_003454:FN1510|3end_hypothetical_protein_14231..14381_NEGATIVE 160 204 161 64 27 NC_003454:FN0398|3end_Dipeptide_transport_system_permease_protein_dppC_1037707..1037857_POSITIVE 159 199 174 37 4 NC_003454:FN2122|3end_Phenylalanyl-tRNA_synthetase_beta_chain_629798..629948_NEGATIVE 155 66 28 76 35 NC_003454:FN1149|3end_ATP-dependent_nuclease_subunit_A_1799277..1799427_NEGATIVE 155 198 162 70 34 NC_003454:FN0200|3end_Biotin_carboxyl_carrier_protein_of_glutaconyl-COA_decarboxylase_826607..826757_POSITIVE 155 138 102 39 1 NC_003454:FN1784|3end_hypothetical_protein_265735..265885_NEGATIVE 153 205 174 79 47 NC_003454:FN1117|3end_LSU_ribosomal_protein_L21P_1760636..1760786_POSITIVE 153 182 150 49 20 NC_003454:FN1538|3end_Arsenical_pump-driving_ATPase_51109..51259_NEGATIVE 152 192 163 48 14 NC_003454:FN1022|3end_Calcium-transporting_ATPase_1677501..1677651_POSITIVE