Origin IGS: aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaaaatcacaaaccagtactttatagactttaaaaaataacctatttcttaaatagtgttgataaaaaatttagacttacaattaacataattaagagaattttttgagaataaaatcttaaaaagttccctttt Mask Tandem Repeat Region ================================================ aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Find is-nt database================================================ Query_seq: FN0812:FN0813|FN0812:FN0813:Hypothetical cytosolic protein:Transcriptional regulator, TetR family:-><-:1471458..1471593 136 aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0812:FN0813|FN0812:FN0813:Hypothetical cytosolic protein:Transcriptional regulator, TetR family:-><-:1471458..1471593 136 aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0812:FN0813|FN0812:FN0813:Hypothetical cytosolic protein:Transcriptional regulator, TetR family:-><-:1471458..1471593 136 aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Predict ORF larger than 30AA ================================================ aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0812:FN0813|FN0812:FN0813:Hypothetical cytosolic protein:Transcriptional regulator, TetR family:-><-:1471458..1471593 136 aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Intra-Species Hit: Count: 1 Min: 1 Max: 136 Len: 136 Subject: NC_003454_FN0812_FN0813|Hypothetical cytosolic protein:Transcriptional regulator, TetR family|POSITIVE:NEGATIVE|[1471458,1471593]|136 HSP 1 e-value: 1.0E-71 bit: 270.0 Len: 136 Query Start:1 Query End:136 Subject Strand: POSITIVE Subject Start: 1 Subject End: 136 aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa aaaagggaactttttaagattttattctcaaaaaattctcttaattatgttaattgtaagtctaaattttttatcaacactatttaagaaataggttattttttaaagtctataaagtactggtttgtgattttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAGGGAACUUUUUAAGAUUUUAUUCUCAAAAAAUUCUCUUAAUUAUGUUAAUUGUAAGUCUAAAUUUUUUAUCAACACUAUUUAAGAAAUAGGUUAUUUUUUAAAGUCUAUAAAGUACUGGUUUGUGAUUUUAA ..(((((((.(((((.(((......))).))))).))))))).........(((..(((..(......((((((..((....(((((((((((...)))))))))))))..))))))....)..)))..))).... (-21.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAAAAUCACAAACCAGUACUUUAUAGACUUUAAAAAAUAACCUAUUUCUUAAAUAGUGUUGAUAAAAAAUUUAGACUUACAAUUAACAUAAUUAAGAGAAUUUUUUGAGAAUAAAAUCUUAAAAAGUUCCCUUUU ...............(((.((....))))).................((((((...((((((((..................))))))))..))))))(((((((((((((......)))))))))))))...... (-18.47) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 38 1 231 192 NC_003454:FN0657|5end_Acetyltransferase_1314266..1314496_POSITIVE 195 64 25 50 11 NC_003454:FN1578|5end_hypothetical_protein_88796..89026_NEGATIVE 189 119 77 193 146 NC_003454:FN0742|5end_hypothetical_protein_1400121..1400351_POSITIVE 184 110 61 111 53 NC_003454:FN0171|5end_Hypothetical_cytosolic_protein_796548..796778_POSITIVE 183 89 44 190 140 NC_003454:FN1750|5end_Pyruvate-flavodoxin_oxidoreductase_247811..248041_POSITIVE 175 57 17 119 77 NC_003454:FN0325|5end_50S_ribosomal_protein_L20_961274..961504_NEGATIVE 173 47 9 108 71 NC_003454:FN1476|5end_N-acetylmannosamine-6-phosphate_2-epimerase_2158672..2158902_POSITIVE 173 79 33 131 92 NC_003454:FN1157|5end_Polypeptide_deformylase_1813903..1814133_POSITIVE 172 76 36 208 169 NC_003454:FN1083|5end_putative_surface_protein_1727963..1728193_POSITIVE 168 63 35 121 95 NC_003454:FN1145|5end_Oligoendopeptidase_F_1795931..1796161_NEGATIVE 166 86 67 204 185 NC_003454:FN0599|5end_Transposase_1253833..1254063_POSITIVE 165 126 92 67 28 NC_003454:FN1277|5end_Aminoacyl-histidine_dipeptidase_1936754..1936984_NEGATIVE 165 86 67 201 182 NC_003454:FN0275|5end_Transposase_901560..901790_NEGATIVE 163 48 3 200 164 NC_003454:FN0708|5end_Hypothetical_cytosolic_protein_1366668..1366898_POSITIVE 163 75 36 195 160 NC_003454:FN0284|5end_16S_rRNA_processing_protein_rimM_913923..914153_NEGATIVE 161 106 61 75 28 NC_003454:FN1317|5end_RNA_polymerase_sigma_factor_1971085..1971315_NEGATIVE 160 26 1 231 204 NC_003454:FN1162|5end_Hydroxyacylglutathione_hydrolase_1820161..1820391_POSITIVE 159 50 1 203 158 NC_003454:FN1075|5end_hypothetical_protein_1722042..1722272_POSITIVE 159 80 39 164 121 NC_003454:FN0108|5end_Microcin_C7_self-immunity_protein_mccF_743219..743449_POSITIVE 158 29 3 164 138 NC_003454:FN1451|5end_cell_division_protein_FtsZ_2132710..2132940_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 500 80 31 52 2 NC_003454:FN0273|3end_Butyrate-acetoacetate_CoA-transferase_subunit_B_900162..900312_POSITIVE 412 50 1 60 13 NC_003454:FN1815|3end_Transposase_295626..295776_NEGATIVE 411 46 1 78 31 NC_003454:FN0656|3end_hypothetical_protein_1314185..1314335_POSITIVE 390 80 31 55 5 NC_003454:FN1872|3end_hypothetical_protein_362789..362939_NEGATIVE 375 49 1 72 21 NC_003454:FN0470|3end_Putative_efflux_pump_component_MtrF_1116359..1116509_POSITIVE 371 70 24 62 17 NC_003454:FN0451|3end_hypothetical_protein_1095427..1095577_POSITIVE 349 48 1 66 16 NC_003454:FN1161|3end_Glutamate_racemase_1820053..1820203_POSITIVE 335 70 21 62 13 NC_003454:FN1752|3end_Regulatory_protein_TENI_248591..248741_NEGATIVE 297 46 1 79 32 NC_003454:FN0792|3end_urocanate_hydratase_1450364..1450514_POSITIVE 289 41 1 79 39 NC_003454:FN1951|3end_ATPase_associated_with_chromosome_architecture/replication_458400..458550_NEGATIVE 205 48 1 85 40 NC_003454:FN1222|3end_hypothetical_protein_1881580..1881730_NEGATIVE 195 64 25 62 23 NC_003454:FN1579|3end_Cysteinyl-tRNA_synthetase_88784..88934_NEGATIVE 185 128 90 46 2 NC_003454:FN0067|3end_Isoleucyl-tRNA_synthetase_707418..707568_POSITIVE 184 110 61 96 38 NC_003454:FN0170|3end_GTP-binding_protein_796613..796763_POSITIVE 180 52 15 93 59 NC_003454:FN0638|3end_hypothetical_protein_1296615..1296765_NEGATIVE 175 57 17 94 52 NC_003454:FN0326|3end_LSU_ribosomal_protein_L35P_961299..961449_NEGATIVE 173 47 9 93 56 NC_003454:FN1475|3end_N-acetylneuraminate_lyase_2158737..2158887_POSITIVE 173 79 33 121 82 NC_003454:FN1156|3end_Primosomal_protein_N'_1813973..1814123_POSITIVE 171 109 67 105 60 NC_003454:FN0120|3end_hypothetical_protein_752718..752868_NEGATIVE 163 99 64 58 24 NC_003454:FN0254|3end_Fusobacterium_outer_membrane_protein_family_881390..881540_POSITIVE