Origin IGS: ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttcctcctataatttaattgtaataccttattctaacataaattttaaaaattttacttatttattttacataacataaaaataatgggatataataacaatcccatcattttagcttattttttaa Mask Tandem Repeat Region ================================================ ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Find is-nt database================================================ Query_seq: FN0774:FN0775|FN0774:FN0775:Hypothetical cytosolic protein:putative aminopeptidase 2:->->:1430579..1430707 129 ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0774:FN0775|FN0774:FN0775:Hypothetical cytosolic protein:putative aminopeptidase 2:->->:1430579..1430707 129 ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0774:FN0775|FN0774:FN0775:Hypothetical cytosolic protein:putative aminopeptidase 2:->->:1430579..1430707 129 ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Predict ORF larger than 30AA ================================================ ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 26 End: 55 .........................ATTGTTATTATATCCCATTATTTTTATGTT.......................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0774:FN0775|FN0774:FN0775:Hypothetical cytosolic protein:putative aminopeptidase 2:->->:1430579..1430707 129 ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 129 Len: 129 Subject: NC_003454_FN0774_FN0775|Hypothetical cytosolic protein:putative aminopeptidase 2|POSITIVE:POSITIVE|[1430579,1430707]|129 HSP 1 e-value: 2.0E-67 bit: 256.0 Len: 129 Query Start:1 Query End:129 Subject Strand: POSITIVE Subject Start: 1 Subject End: 129 ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa ttaaaaaataagctaaaatgatgggattgttattatatcccattatttttatgttatgtaaaataaataagtaaaatttttaaaatttatgttagaataaggtattacaattaaattataggaggaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAAAAAAUAAGCUAAAAUGAUGGGAUUGUUAUUAUAUCCCAUUAUUUUUAUGUUAUGUAAAAUAAAUAAGUAAAAUUUUUAAAAUUUAUGUUAGAAUAAGGUAUUACAAUUAAAUUAUAGGAGGAAAA ..........(((.(((((((((((((.........)))))))))))))...))).....................((((((.((((((.(((..((((...))))..))))))))).))))))..... (-18.20) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCCUCCUAUAAUUUAAUUGUAAUACCUUAUUCUAACAUAAAUUUUAAAAAUUUUACUUAUUUAUUUUACAUAACAUAAAAAUAAUGGGAUAUAAUAACAAUCCCAUCAUUUUAGCUUAUUUUUUAA ................................................................................(((((.(((((((.........))))))).))))).............. (-10.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 215 56 11 153 109 NC_003454:FN0117|5end_O6-methylguanine-DNA_methyltransferase_750338..750568_POSITIVE 211 56 20 95 63 NC_003454:FN0680|5end_Ribulose-phosphate_3-epimerase_1335455..1335685_POSITIVE 195 52 22 99 68 NC_003454:FN1100|5end_Plasmid_addiction_system_poison_protein_1744177..1744407_POSITIVE 189 50 5 70 28 NC_003454:FN0377|5end_Iron(III)-transport_system_permease_protein_sfuB_1007464..1007694_POSITIVE 182 82 51 108 73 NC_003454:FN0385|5end_hypothetical_protein_1017920..1018150_POSITIVE 179 70 25 110 64 NC_003454:FN0184|5end_hypothetical_protein_809087..809317_NEGATIVE 177 46 21 27 2 NC_003454:FN1908|5end_Glycerophosphoryl_diester_phosphodiesterase_401669..401899_NEGATIVE 172 109 67 178 136 NC_003454:FN0421|5end_carbamoyl-phosphate_synthase_small_subunit_1061936..1062166_POSITIVE 171 72 33 207 167 NC_003454:FN0999|5end_Deblocking_aminopeptidase_1653754..1653984_POSITIVE 170 50 20 97 66 NC_003454:FN0013|5end_Transporter_652218..652448_POSITIVE 163 60 11 133 86 NC_003454:FN1434|5end_Tetratricopeptide_repeat_family_protein_2104511..2104741_NEGATIVE 163 45 5 143 103 NC_003454:FN0939|5end_Hypothetical_membrane-spanning_protein_1592919..1593149_NEGATIVE 163 60 30 54 15 NC_003454:FN0683|5end_hypothetical_protein_1338759..1338989_NEGATIVE 162 59 29 45 15 NC_003454:FN1873|5end_Bis(5'-nucleosyl)-tetraphosphatase_363440..363670_NEGATIVE 161 55 14 231 178 NC_003454:FN1026|5end_tRNA_pseudouridine_synthase_A_1680823..1681053_NEGATIVE 161 57 19 96 40 NC_003454:FN1533|5end_Electron_transfer_flavoprotein_alpha-subunit_46321..46551_NEGATIVE 159 44 1 161 110 NC_003454:FN1403|5end_Histidine_permease_2061743..2061973_POSITIVE 159 59 11 122 67 NC_003454:FN0779|5end_Putative_GTPase_(G3E_family)_1436980..1437210_NEGATIVE 158 45 7 35 3 NC_003454:FN1795|5end_hypothetical_protein_276578..276808_NEGATIVE 157 55 13 176 127 NC_003454:FN0862|5end_hypothetical_protein_1522371..1522601_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 218 49 15 76 42 NC_003454:FN0774|3end_Hypothetical_cytosolic_protein_1430518..1430668_POSITIVE 211 56 20 93 61 NC_003454:FN0679|3end_GTPase_1335533..1335683_POSITIVE 195 52 22 114 83 NC_003454:FN1099|3end_hypothetical_protein_1744272..1744422_POSITIVE 189 50 5 86 44 NC_003454:FN0376|3end_Iron(III)-transport_ATP-binding_protein_sfuC_1007560..1007710_POSITIVE 182 82 51 77 42 NC_003454:FN0384|3end_hypothetical_protein_1017969..1018119_POSITIVE 175 57 12 90 48 NC_003454:FN1199|3end_DNA_polymerase_IV_1860193..1860343_POSITIVE 163 45 5 87 47 NC_003454:FN0940|3end_hypothetical_protein_1592975..1593125_NEGATIVE 162 55 23 111 76 NC_003454:FN1168|3end_Macrolide-efflux_protein_1826651..1826801_NEGATIVE 162 59 29 32 2 NC_003454:FN1874|3end_Ribose_5-phosphate_isomerase_363453..363603_NEGATIVE 161 57 19 84 28 NC_003454:FN1534|3end_Electron_transfer_flavoprotein_beta-subunit_46333..46483_NEGATIVE 159 59 11 103 48 NC_003454:FN0780|3end_Hypothetical_membrane-spanning_protein_1436999..1437149_NEGATIVE 158 49 3 89 39 NC_003454:FN0721|3end_hypothetical_protein_1377633..1377783_NEGATIVE 158 60 13 110 65 NC_003454:FN0388|3end_Tetracenomycin_polyketide_synthesis_O-methyltransferase_tcmP_1026600..1026750_NEGATIVE 157 55 13 149 100 NC_003454:FN0863|3end_hypothetical_protein_1522398..1522548_NEGATIVE 157 40 5 67 26 NC_003454:FN0533|3end_Hypothetical_membrane-spanning_protein_1187601..1187751_NEGATIVE 155 45 5 93 38 NC_003454:FN1931|3end_Protease_430259..430409_NEGATIVE 153 41 8 71 39 NC_003454:FN2120|3end_Hypothetical_exported_24-amino_acid_repeat_protein_628361..628511_NEGATIVE 153 52 22 60 25 NC_003454:FN1952|3end_Na(+)-linked_D-alanine_glycine_permease_458943..459093_NEGATIVE 148 59 17 109 54 NC_003454:FN1340|3end_Glutamyl-tRNA_synthetase_1993353..1993503_NEGATIVE 148 56 11 101 60 NC_003454:FN1990|3end_Tetratricopeptide_repeat_protein_494692..494842_POSITIVE