Origin IGS: aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattcagcaaaatttttcttaattcttgaaatcatacaattcctcctttcaataaaataacatatgttatattatatcagtatattgaaaatttgcaagaaatatttttaaataaaaaaaactattgtaaattaaatttttacaatagcttctactatattcttttaaaaattttt Mask Tandem Repeat Region ================================================ aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Find is-nt database================================================ Query_seq: FN0662:FN0663|FN0662:FN0663:Formiminoglutamase:hypothetical protein:->->:1319249..1319425 177 aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0662:FN0663|FN0662:FN0663:Formiminoglutamase:hypothetical protein:->->:1319249..1319425 177 aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0662:FN0663|FN0662:FN0663:Formiminoglutamase:hypothetical protein:->->:1319249..1319425 177 aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Predict ORF larger than 30AA ================================================ Protein_Len: 46 Strand: - Start: 1 End: 138 F I K L L I Y Y F S N Y F N L K C Y N K K N L F I E Q L N E I Y Q Y L I V Y T I K N F P P M ....................................... aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 48 HP_score: -3.9 Tail_Score: -3.6292 Start: 20 End: 64 Full_Region: ATTTTTAAAAGAATA TAGTAGAAGCTATTGTAAA AATTTAA TTTACAATAGTTTTTTTTA TTTAAAAATATTTCT ...................TAGTAGAAGCTATTGTAAAAATTTAATTTACAATAGTTTTTTTTA................................................................................................................. TransTerm Strand: - Conf: 44 HP_score: -3.3 Tail_Score: -3.54149 Start: 28 End: 56 Full_Region: ttttaaataaaaaaa actattgtaaa ttaaatt tttacaatagc ttctactatattctt ...........................actattgtaaattaaatttttacaatagc......................................................................................................................... TransTerm Strand: - Conf: 59 HP_score: -6.8 Tail_Score: -2.71405 Start: 29 End: 55 Full_Region: tttaaataaaaaaaa ctattgtaaa ttaaatt tttacaatag cttctactatattct ............................ctattgtaaattaaatttttacaatag.......................................................................................................................... Find igs database================================================ Query_seq: FN0662:FN0663|FN0662:FN0663:Formiminoglutamase:hypothetical protein:->->:1319249..1319425 177 aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Intra-Species Hit: Count: 1 Min: 1 Max: 177 Len: 177 Subject: NC_003454_FN0662_FN0663|Formiminoglutamase:hypothetical protein|POSITIVE:POSITIVE|[1319249,1319425]|177 HSP 1 e-value: 1.0E-81 bit: 303.0 Len: 177 Query Start:1 Query End:177 Subject Strand: POSITIVE Subject Start: 1 Subject End: 177 aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagnnnnnnnnatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta aaaaatttttaaaagaatatagtagaagctattgtaaaaatttaatttacaatagttttttttatttaaaaatatttcttgcaaattttcaatatactgatataatataacatatgttattttattgaaaggaggaattgtatgatttcaagaattaagaaaaattttgctgaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAUUUUUAAAAGAAUAUAGUAGAAGCUAUUGUAAAAAUUUAAUUUACAAUAGUUUUUUUUAUUUAAAAAUAUUUCUUGCAAAUUUUCAAUAUACUGAUAUAAUAUAACAUAUGUUAUUUUAUUGAAAGGAGGAAUUGUAUGAUUUCAAGAAUUAAGAAAAAUUUUGCUGAAUUA ..............(((((....(((((((((((((((.......)))))))))))))))..))))).(((((.(((((((....(((((((((...(((((((........)))))))...)))))))))..((((((....))))))......))))))).)))))......... (-34.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCAGCAAAAUUUUUCUUAAUUCUUGAAAUCAUACAAUUCCUCCUUUCAAUAAAAUAACAUAUGUUAUAUUAUAUCAGUAUAUUGAAAAUUUGCAAGAAAUAUUUUUAAAUAAAAAAAACUAUUGUAAAUUAAAUUUUUACAAUAGCUUCUACUAUAUUCUUUUAAAAAUUUUU .........(((((((((.....((((((((((...................((((.(((((....))))).)))).((((....))))..)))).))))))....................((((((((((.......))))))))))..................))))))))). (-21.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 280 65 24 228 190 NC_003454:FN0093|5end_Thioredoxin_729169..729399_POSITIVE 275 61 24 211 173 NC_003454:FN1949|5end_Xaa-Pro_dipeptidase_454922..455152_NEGATIVE 270 67 24 195 151 NC_003454:FN0462|5end_DNA_mismatch_repair_protein_mutL_1107184..1107414_POSITIVE 252 57 13 227 188 NC_003454:FN0165|5end_hypothetical_protein_793491..793721_POSITIVE 225 63 24 73 24 NC_003454:FN2068|5end_dGTP_triphosphohydrolase_578986..579216_NEGATIVE 214 58 16 70 28 NC_003454:FN0699|5end_Protein_translocase_subunit_secD_1357051..1357281_POSITIVE 199 37 13 45 16 NC_003454:FN0716|5end_Phophatidylinositol-4-phosphate_5-kinase_1373969..1374199_POSITIVE 192 61 24 138 102 NC_003454:FN0626|5end_Hypothetical_cytosolic_protein_1286092..1286322_NEGATIVE 190 161 129 70 33 NC_003454:FN1425|5end_hypothetical_protein_2091911..2092141_POSITIVE 189 99 52 60 17 NC_003454:FN1077|5end_hypothetical_protein_1724005..1724235_NEGATIVE 188 160 111 80 22 NC_003454:FN2005|5end_Cobalt_transport_ATP-binding_protein_cbiO_505081..505311_POSITIVE 186 57 18 198 162 NC_003454:FN0799|5end_Isoamylase_1459826..1460056_NEGATIVE 177 114 77 104 65 NC_003454:FN0091|5end_Phosphoserine_phosphatase_726788..727018_POSITIVE 176 143 106 223 190 NC_003454:FN0633|5end_Replication_protein_1290928..1291158_POSITIVE 176 99 63 198 166 NC_003454:FN1532|5end_murein_hydrolase_exporter_45319..45549_NEGATIVE 172 137 98 82 46 NC_003454:FN1288|5end_hypothetical_protein_1944580..1944810_POSITIVE 170 150 108 213 166 NC_003454:FN1448|5end_Hypothetical_cytosolic_protein_2118428..2118658_NEGATIVE 169 79 31 206 150 NC_003454:FN0847|5end_TPR-repeat-containing_proteins_1505253..1505483_POSITIVE 169 96 66 120 91 NC_003454:FN0452|5end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing]_1095699..1095929_POSITIVE 168 67 24 228 183 NC_003454:FN1326|5end_Undecaprenyl_pyrophosphate_synthetase_1981890..1982120_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 280 65 24 85 47 NC_003454:FN0092|3end_NADPH-dependent_butanol_dehydrogenase_729106..729256_POSITIVE 275 61 24 70 32 NC_003454:FN1950|3end_Serine_protease_455063..455213_NEGATIVE 270 67 24 78 34 NC_003454:FN0461|3end_Probable_sigma(54)_modulation_protein_1107147..1107297_POSITIVE 232 63 21 82 41 NC_003454:FN0702|3end_N-acyl-L-amino_acid_amidohydrolase_1360925..1361075_NEGATIVE 230 59 14 84 43 NC_003454:FN1080|3end_Export_ABC_transporter_1727308..1727458_POSITIVE 229 60 24 67 31 NC_003454:FN0662|3end_Formiminoglutamase_1319188..1319338_POSITIVE 214 58 16 46 4 NC_003454:FN0698|3end_DNA_integration/recombination/invertion_protein_1357107..1357257_POSITIVE 210 67 21 96 50 NC_003454:FN1277|3end_Aminoacyl-histidine_dipeptidase_1935294..1935444_NEGATIVE 206 57 16 84 34 NC_003454:FN0315|3end_Transcriptional_regulator,_AraC_family_952859..953009_POSITIVE 200 60 12 104 62 NC_003454:FN1044|3end_hypothetical_protein_1694453..1694603_NEGATIVE 199 37 13 52 23 NC_003454:FN0715|3end_hypothetical_protein_1374056..1374206_POSITIVE 197 69 24 98 55 NC_003454:FN2001|3end_hypothetical_protein_501298..501448_NEGATIVE 195 69 21 90 37 NC_003454:FN0636|3end_hypothetical_protein_1295609..1295759_POSITIVE 195 62 21 80 38 NC_003454:FN0146|3end_hypothetical_protein_775330..775480_POSITIVE 192 61 24 75 39 NC_003454:FN0627|3end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing]_1286155..1286305_NEGATIVE 188 160 111 96 38 NC_003454:FN2004|3end_Cobalt_transport_ATP-binding_protein_cbiO_505177..505327_POSITIVE 187 58 14 45 7 NC_003454:FN1391|3end_Acetyltransferase_2049133..2049283_POSITIVE 187 64 24 89 56 NC_003454:FN0039|3end_DNA_primase_(bacterial_type)_and_small_primase-like_proteins_678661..678811_NEGATIVE 186 60 12 81 32 NC_003454:FN1037|3end_flavodoxin_1689973..1690123_POSITIVE 186 57 18 77 41 NC_003454:FN0800|3end_Amino_acid-binding_protein_1459947..1460097_NEGATIVE