Origin IGS: aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttcttgaaaaagtttttaatatggaaggataaaaatgataaataatgtccaagaaagtaataggacaaaattacctcccttgccatttaatagaaacatttttcatattagacaatataaattcagaaaaaacaattaaagttaaaggaggaattt Mask Tandem Repeat Region ================================================ aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Find is-nt database================================================ Query_seq: FN0638:FN0639|FN0638:FN0639:hypothetical protein:hypothetical protein:<-<-:1297065..1297220 156 aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0638:FN0639|FN0638:FN0639:hypothetical protein:hypothetical protein:<-<-:1297065..1297220 156 aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0638:FN0639|FN0638:FN0639:hypothetical protein:hypothetical protein:<-<-:1297065..1297220 156 aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 21 End: 128 .................... N N K R F K Y Q R I H F I N R N F P L P P L K T R N S E Q V N N I M K M ............................ Protein_Len: 38 Strand: - Start: 2 End: 115 . I G G K V K I T K E S N I N D L I F F T E I L H C P L Y N Q G I V K K S M M ......................................... aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0638:FN0639|FN0638:FN0639:hypothetical protein:hypothetical protein:<-<-:1297065..1297220 156 aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Intra-Species Hit: Count: 1 Min: 1 Max: 156 Len: 156 Subject: NC_003454_FN0638_FN0639|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1297065,1297220]|156 HSP 1 e-value: 1.0E-83 bit: 309.0 Len: 156 Query Start:1 Query End:156 Subject Strand: POSITIVE Subject Start: 1 Subject End: 156 aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa aaattcctcctttaactttaattgttttttctgaatttatattgtctaatatgaaaaatgtttctattaaatggcaagggaggtaattttgtcctattactttcttggacattatttatcatttttatccttccatattaaaaactttttcaagaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAUUCCUCCUUUAACUUUAAUUGUUUUUUCUGAAUUUAUAUUGUCUAAUAUGAAAAAUGUUUCUAUUAAAUGGCAAGGGAGGUAAUUUUGUCCUAUUACUUUCUUGGACAUUAUUUAUCAUUUUUAUCCUUCCAUAUUAAAAACUUUUUCAAGAA ((((((.......(((.......)))......)))))).............(((((((.((((...((((((((.(((((.(((((...(((((............)))))....))))).......))))))))).))))))))))))))).... (-23.92) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCUUGAAAAAGUUUUUAAUAUGGAAGGAUAAAAAUGAUAAAUAAUGUCCAAGAAAGUAAUAGGACAAAAUUACCUCCCUUGCCAUUUAAUAGAAACAUUUUUCAUAUUAGACAAUAUAAAUUCAGAAAAAACAAUUAAAGUUAAAGGAGGAAUUU ....((((((((((((((..((((((((......((.....))..(((((............)))))..........)))).))))....))))))).))))))).............((((((......(((.......))).......)))))) (-22.92) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 805 120 71 211 162 NC_003454:FN0638|5end_hypothetical_protein_1296974..1297204_NEGATIVE 276 93 61 214 180 NC_003454:FN0763|5end_Transporter,_LysE_family_1420169..1420399_POSITIVE 229 47 4 151 103 NC_003454:FN2123|5end_Phenylalanyl-tRNA_synthetase_alpha_chain_633224..633454_NEGATIVE 228 106 65 107 68 NC_003454:FN1558|5end_30S_ribosomal_protein_S12_80518..80748_NEGATIVE 222 150 104 130 81 NC_003454:FN0395|5end_Transporter_1033106..1033336_POSITIVE 217 136 100 140 106 NC_003454:FN1205|5end_Protease_1865162..1865392_NEGATIVE 215 134 92 226 182 NC_003454:FN0290|5end_Hemolysin_918997..919227_NEGATIVE 215 138 92 173 125 NC_003454:FN1719|5end_hypothetical_protein_217948..218178_POSITIVE 214 135 92 105 59 NC_003454:FN1083|5end_putative_surface_protein_1727963..1728193_POSITIVE 213 48 4 219 174 NC_003454:FN1495|5end_hypothetical_protein_2174410..2174640_NEGATIVE 213 48 4 142 97 NC_003454:FN1493|5end_hypothetical_protein_2174063..2174293_NEGATIVE 209 134 94 102 70 NC_003454:FN2016|5end_ATP-dependent_Clp_protease_proteolytic_subunit_518848..519078_NEGATIVE 206 109 72 220 176 NC_003454:FN1690|5end_hypothetical_protein_186389..186619_NEGATIVE 204 136 94 104 67 NC_003454:FN0896|5end_hypothetical_protein_1555780..1556010_NEGATIVE 201 47 8 143 105 NC_003454:FN0845|5end_hypothetical_protein_1503935..1504165_POSITIVE 198 139 94 184 130 NC_003454:FN0701|5end_Methyltransferase_1359261..1359491_POSITIVE 197 136 101 123 80 NC_003454:FN0088|5end_hypothetical_protein_724887..725117_POSITIVE 196 139 91 146 100 NC_003454:FN1236|5end_Choline_transport_protein_1893480..1893710_NEGATIVE 196 135 91 112 61 NC_003454:FN0753|5end_aspartyl/glutamyl-tRNA_amidotransferase_subunit_B_1413169..1413399_NEGATIVE 196 69 23 220 173 NC_003454:FN0744|5end_metal_dependent_phosphohydrolase_1403072..1403302_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 229 47 4 124 76 NC_003454:FN2124|3end_Hypothetical_protein_with_Metallo-phosphoesterase_motif_633251..633401_NEGATIVE 222 150 104 91 42 NC_003454:FN0394|3end_Outer_membrane_protein_1033147..1033297_POSITIVE 217 136 100 89 55 NC_003454:FN1206|3end_Hemolysin_1865213..1865363_NEGATIVE 215 138 92 133 85 NC_003454:FN1718|3end_translocase_217988..218138_POSITIVE 214 135 92 108 62 NC_003454:FN1082|3end_hypothetical_protein_1728046..1728196_POSITIVE 213 48 4 142 97 NC_003454:FN1494|3end_Rod_shape-determining_protein_mreC_2174063..2174213_NEGATIVE 213 48 4 142 97 NC_003454:FN1496|3end_Rod_shape-determining_protein_mreC_-13..137_NEGATIVE 204 106 70 104 65 NC_003454:FN0261|3end_Pyruvate_formate-lyase_activating_enzyme_886779..886929_NEGATIVE 197 136 101 110 67 NC_003454:FN0087|3end_Ethanolamine_utilization_protein_eutN_724954..725104_POSITIVE 196 135 91 98 47 NC_003454:FN0754|3end_Glutamyl-tRNA(Gln)_amidotransferase_subunit_A_1413183..1413333_NEGATIVE 196 130 84 139 92 NC_003454:FN0065|3end_Transcription_accessory_protein_(S1_RNA_binding_domain)_702184..702334_POSITIVE 196 95 60 69 22 NC_003454:FN1736|3end_V-type_sodium_ATP_synthase_subunit_A_235413..235563_NEGATIVE 194 67 28 147 114 NC_003454:FN0849|3end_8-amino-7-oxononanoate_synthase_1508992..1509142_POSITIVE 193 43 8 43 11 NC_003454:FN0182|3end_Sarcosine_oxidase_alpha_subunit_805801..805951_NEGATIVE 193 136 94 113 70 NC_003454:FN1687|3end_Gluconate_5-dehydrogenase_182900..183050_NEGATIVE 193 50 1 118 70 NC_003454:FN1614|3end_MG(2+)_chelatase_family_protein_123509..123659_NEGATIVE 193 48 4 93 51 NC_003454:FN1539|3end_Iron-sulfur_cluster-binding_protein_53092..53242_POSITIVE 192 50 6 115 76 NC_003454:FN0361|3end_ATP_synthase_delta_chain,_sodium_ion_specific_992381..992531_NEGATIVE 192 136 100 117 78 NC_003454:FN0101|3end_Glutaredoxin_736313..736463_POSITIVE 192 130 81 97 46 NC_003454:FN1563|3end_hypothetical_protein_84777..84927_NEGATIVE