Origin IGS: tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atagttacctcctatacactaaacaaatctaactttatcatggtagattttttaatctttttcagtatattttctaaaattaaaaagggatattaaaaatttttttatttctaattccccctttaaaatcttaaata Mask Tandem Repeat Region ================================================ tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Find is-nt database================================================ Query_seq: FN0612:FN0613|FN0612:FN0613:hypothetical protein:hypothetical protein:->->:1270893..1271029 137 tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0612:FN0613|FN0612:FN0613:hypothetical protein:hypothetical protein:->->:1270893..1271029 137 tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0612:FN0613|FN0612:FN0613:hypothetical protein:hypothetical protein:->->:1270893..1271029 137 tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 45 End: 137 ............................................ Y G K K I K S F I S F F I L F D V M I F N S K N L T Y S T V M tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0612:FN0613|FN0612:FN0613:hypothetical protein:hypothetical protein:->->:1270893..1271029 137 tatttaagattttaaagggggaattagaaataaaaaaatttttaatatccctttttaattttagaaaatatactgaaaaagattaaaaaatctaccatgataaagttagatttgtttagtgtataggaggtaactat Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: NC_003454_FN0612_FN0613|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1270893,1271029]|137 HSP 1 e-value: 6.0E-18 bit: 91.7 Len: 46 Query Start:92 Query End:137 Subject Strand: POSITIVE Subject Start: 92 Subject End: 137 ...........................................................................................ctaccatgataaagttagatttgtttagtgtataggaggtaactat ...........................................................................................ctaccatgataaagttagatttgtttagtgtataggaggtaactat HSP 2 e-value: 1.0E-6 bit: 54.0 Len: 27 Query Start:1 Query End:27 Subject Strand: POSITIVE Subject Start: 1 Subject End: 27 tatttaagattttaaagggggaattag.............................................................................................................. tatttaagattttaaagggggaattag.............................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUUAAGAUUUUAAAGGGGGAAUUAGAAAUAAAAAAAUUUUUAAUAUCCCUUUUUAAUUUUAGAAAAUAUACUGAAAAAGAUUAAAAAAUCUACCAUGAUAAAGUUAGAUUUGUUUAGUGUAUAGGAGGUAACUAU ..(((((((((..(((((((..(((((((((......))))))))).)))))))..)))))))))..((((((((((..........(((((((.(........).))))))).))))))))))............. (-31.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGUUACCUCCUAUACACUAAACAAAUCUAACUUUAUCAUGGUAGAUUUUUUAAUCUUUUUCAGUAUAUUUUCUAAAAUUAAAAAGGGAUAUUAAAAAUUUUUUUAUUUCUAAUUCCCCCUUUAAAAUCUUAAAUA ...................((((.(((((((.((.......))))))))))))).........................(((((..((((.((((.((((......)))).))))))))..)))))........... (-13.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 221 31 9 22 1 NC_003454:FN1068|5end_Smf_protein_1713811..1714041_POSITIVE 218 58 13 206 162 NC_003454:FN0614|5end_Export_ABC_transporter_1271447..1271677_POSITIVE 211 40 14 229 201 NC_003454:FN0238|5end_hypothetical_protein_863314..863544_NEGATIVE 191 42 1 220 178 NC_003454:FN1134|5end_Hypothetical_cytosolic_protein_1781495..1781725_POSITIVE 180 68 38 190 156 NC_003454:FN1584|5end_Beta-lactamase_96158..96388_NEGATIVE 178 54 15 54 3 NC_003454:FN0973|5end_Histidinol-phosphate_aminotransferase_1626699..1626929_NEGATIVE 178 52 15 148 109 NC_003454:FN0184|5end_hypothetical_protein_809087..809317_NEGATIVE 170 56 12 170 135 NC_003454:FN0063|5end_hypothetical_protein_698982..699212_POSITIVE 166 129 90 196 146 NC_003454:FN0427|5end_Orotate_phosphoribosyltransferase_1069405..1069635_POSITIVE 166 81 48 45 17 NC_003454:FN0085|5end_Ethanolamine_utilization_cobalamin_adenosyltransferase_723270..723500_POSITIVE 159 75 38 152 111 NC_003454:FN1365|5end_GTP-binding_protein_2016739..2016969_NEGATIVE 158 125 104 205 183 NC_003454:FN1499|5end_Cell_surface_protein_3647..3877_POSITIVE 157 116 90 208 181 NC_003454:FN2079|5end_ABC_transporter_ATP-binding_protein_591118..591348_NEGATIVE 156 75 40 177 148 NC_003454:FN0583|5end_hypothetical_protein_1239001..1239231_POSITIVE 151 30 3 194 167 NC_003454:FN1262|5end_Integral_membrane_protein_1918043..1918273_POSITIVE 151 31 7 62 43 NC_003454:FN0051|5end_hypothetical_protein_688929..689159_NEGATIVE 150 127 86 132 92 NC_003454:FN1120|5end_phosphoenolpyruvate_carboxykinase_1761438..1761668_POSITIVE 149 51 14 143 108 NC_003454:FN0248|5end_hypothetical_protein_871562..871792_POSITIVE 148 120 73 47 4 NC_003454:FN1405|5end_Formiminotetrahydrofolate_cyclodeaminase_2064287..2064517_POSITIVE 148 78 39 128 92 NC_003454:FN1289|5end_hypothetical_protein_1946447..1946677_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 278 58 12 79 33 NC_003454:FN0612|3end_hypothetical_protein_1270832..1270982_POSITIVE 225 52 14 71 40 NC_003454:FN0395|3end_Transporter_1034046..1034196_POSITIVE 208 50 10 84 41 NC_003454:FN0736|3end_Methyltransferase_1394658..1394808_NEGATIVE 180 68 38 135 101 NC_003454:FN1585|3end_hypothetical_protein_96213..96363_NEGATIVE 172 77 38 130 93 NC_003454:FN1516|3end_hypothetical_protein_20664..20814_NEGATIVE 170 56 12 83 48 NC_003454:FN0062|3end_Hypothetical_cytosolic_protein_698975..699125_POSITIVE 169 79 40 126 89 NC_003454:FN1168|3end_Macrolide-efflux_protein_1826651..1826801_NEGATIVE 159 75 38 128 87 NC_003454:FN1366|3end_Triosephosphate_isomerase_2016763..2016913_NEGATIVE 157 125 85 81 45 NC_003454:FN0093|3end_Thioredoxin_729560..729710_POSITIVE 156 75 40 123 94 NC_003454:FN0582|3end_Lipoprotein_releasing_system_ATP-binding_protein_lolD_1239027..1239177_POSITIVE 152 105 62 55 9 NC_003454:FN0682|3end_Fibronectin-binding_protein-like_protein_A_1338499..1338649_POSITIVE 149 51 14 79 44 NC_003454:FN0247|3end_Hypothetical_cytosolic_protein_871578..871728_POSITIVE 148 120 73 46 3 NC_003454:FN1404|3end_Imidazolonepropionase_2064366..2064516_POSITIVE 148 78 39 131 95 NC_003454:FN1290|3end_hypothetical_protein_1946444..1946594_NEGATIVE 148 62 38 123 101 NC_003454:FN0802|3end_Amino_acid_transport_system_permease_protein_1461426..1461576_NEGATIVE 147 116 80 92 56 NC_003454:FN1351|3end_15_kDa_lipoprotein_precursor_2002950..2003100_NEGATIVE 147 51 16 61 31 NC_003454:FN1253|3end_hypothetical_protein_1910229..1910379_NEGATIVE 147 82 47 135 102 NC_003454:FN1014|3end_Dihydrofolate_synthase_1668354..1668504_NEGATIVE 147 130 86 72 28 NC_003454:FN0894|3end_hypothetical_protein_1554893..1555043_POSITIVE 146 89 50 83 40 NC_003454:FN1039|3end_Branched-chain_amino_acid_transport_protein_azlC_1691829..1691979_POSITIVE