Origin IGS: gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagaatgagagattttattaaagaattaagaaataatacaaataaagagaaaattattatcactcaaaatggaagtgaattatattttaaaaagggaaaaattgataataaattttttgctataaccaatgggacaacacaagaatctttatattatggagatgaattaagatttaatgtccctactcctaagggattaaaaaatgaattattagaattgactgtacctataagaaaaaaaggaaaacctgtttttgttataagttatggtaaaggtaagaaaaaaagagaattt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aaattctctttttttcttacctttaccataacttataacaaaaacaggttttcctttttttcttataggtacagtcaattctaataattcattttttaatcccttaggagtagggacattaaatcttaattcatctccataatataaagattcttgtgttgtcccattggttatagcaaaaaatttattatcaatttttccctttttaaaatataattcacttccattttgagtgataataattttctctttatttgtattatttcttaattctttaataaaatctctcattctttctttatatatattatttactgctgaaaaagaaaaacagcttatagaaaacaataataataaaatatattttttaatattaatcaccccttaacctaatataaaagtattataacatattttttcttgaaaatttaaagtaatgggacaaatattcttttatagtattgatttagaacataaaaatattaaaattcctaaaaaaatatattgacaaataaaataattctctttactattgattgaaattaaaaaaatttgaaaggagaaataataatgaaaaaattattagtc Mask Tandem Repeat Region ================================================ gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagaatgagagattttattaaagaattaagaaataatacaaataaagagaaaattattatcactcaaaatggaagtgaattatattttaaaaagggaaaaattgataataaattttttgctataaccaatgggacaacacaagaatctttatattatggagatgaattaagatttaatgtccctactcctaagggattaaaaaatgaattattagaattgactgtacctataagaaaaaaaggaaaacctgtttttgttataagttatggtaaaggtaagaaaaaaagagaattt Find is-nt database================================================ Query_seq: FN0499:FN0500|FN0499:FN0500:Hemin receptor:hypothetical protein:<-->:1148495..1149082 588 gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagaatgagagattttattaaagaattaagaaataatacaaataaagagaaaattattatcactcaaaatggaagtgaattatattttaaaaagggaaaaattgataataaattttttgctataaccaatgggacaacacaagaatctttatattatggagatgaattaagatttaatgtccctactcctaagggattaaaaaatgaattattagaattgactgtacctataagaaaaaaaggaaaacctgtttttgttataagttatggtaaaggtaagaaaaaaagagaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0499:FN0500|FN0499:FN0500:Hemin receptor:hypothetical protein:<-->:1148495..1149082 588 gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagaatgagagattttattaaagaattaagaaataatacaaataaagagaaaattattatcactcaaaatggaagtgaattatattttaaaaagggaaaaattgataataaattttttgctataaccaatgggacaacacaagaatctttatattatggagatgaattaagatttaatgtccctactcctaagggattaaaaaatgaattattagaattgactgtacctataagaaaaaaaggaaaacctgtttttgttataagttatggtaaaggtaagaaaaaaagagaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0499:FN0500|FN0499:FN0500:Hemin receptor:hypothetical protein:<-->:1148495..1149082 588 gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagaatgagagattttattaaagaattaagaaataatacaaataaagagaaaattattatcactcaaaatggaagtgaattatattttaaaaagggaaaaattgataataaattttttgctataaccaatgggacaacacaagaatctttatattatggagatgaattaagatttaatgtccctactcctaagggattaaaaaatgaattattagaattgactgtacctataagaaaaaaaggaaaacctgtttttgttataagttatggtaaaggtaagaaaaaaagagaattt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 2 Min: 298 Max: 588 Len: 291 Subject: UniRef90_Q7P6Z7 Cluster: Hypothetical protein FNV1739; n=1; Fusobacterium nucleatum subsp. vincentii ATCC 49256|Rep: Hypothetical protein FNV1739 - Fusobacterium nucleatum subsp. vincentii ATCC 49256 HSP 1 e-value: 6.0E-42 bit: 172.0 Len: 291 Query Start:298 Query End:588 Subject Strand: null Subject Start: 1 Subject End: 97 ......................................................................................................................................................................................................................................................................................................... M R D F I K E L R N N T N K E K I I I T Q N G S E L Y F K K G K I D N K F F A I T N G T T Q E S L Y Y G D E L R F N V P T P K G L K N E L L E L T V P I R K K G K P V F V I S Y G K G K K K R E F ......................................................................................................................................................................................................................................................................................................... M K D F I K E I R D S T N K E K I I I T Q N G N E L Y F K N G K I D S K F F A I T N G T T Q E S L Y Y G D V L R F N V P T A K G L K N E L L E L T V P I R K N G K P I F V I N Y G K G Q K K I D F Subject: UniRef90_Q7P8A9 Cluster: Hypothetical protein FNV2119; n=2; Fusobacterium nucleatum|Rep: Hypothetical protein FNV2119 - Fusobacterium nucleatum subsp. vincentii ATCC 49256 HSP 1 e-value: 5.0E-12 bit: 73.2 Len: 171 Query Start:415 Query End:585 Subject Strand: null Subject Start: 1 Subject End: 57 .............................................................................................................................................................................................................................................................................................................................................................................................................................. I T N G T T Q E S L Y Y G D E L R F N V P T P K G L K N E L L E L T V P I R K K G K P V F V I S Y G K G K K K R E ... .............................................................................................................................................................................................................................................................................................................................................................................................................................. M T D G T T Q E S L F Y G D E L K F N T L T S P K L K K E L L D M L I P I R Q A G K V V L T I N Y G K G E K T K K ... gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict ORF larger than 30AA ================================================ Protein_Len: 32 Strand: + Start: 37 End: 132 .................................... M F L I S I N S K E N Y F I C Q Y I F L G I L I F L C S K S I L ........................................................................................................................................................................................................................................................................................................................................................................................................................................................................ Protein_Len: 30 Strand: + Start: 326 End: 415 ..................................................................................................................................................................................................................................................................................................................................... M I Q I K R K L L S L K M E V N Y I L K R E K L I I N F L L ............................................................................................................................................................................. Protein_Len: 60 Strand: + Start: 208 End: 588 ............................................................................................................................................................................................................... M I N I K K Y I L L L L F S I S C F S F S A V N N I Y K E R M R D F I K E L R N N T N K E K I I I T Q N G S E L Y F K K Protein_Len: 35 Strand: - Start: 450 End: 554 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................. P S S N L N L T G V G L P N F F S N N S N V T G I L F F P F G T K T M .................................. Protein_Len: 51 Strand: - Start: 204 End: 356 ........................................................................................................................................................................................................... P T I L I L F Y I K N N N N E I L Q K E K E A T F L I Y L S L I L S K I L S N L F L V F L S F I I I M ........................................................................................................................................................................................................................................ gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 90 Start: 56 End: 85 .......................................................ATTGACAAATAAAATAATTCTCTTTACTAT....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0499:FN0500|FN0499:FN0500:Hemin receptor:hypothetical protein:<-->:1148495..1149082 588 gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagagaattattttatttgtcaatatatttttttaggaattttaatatttttatgttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatgttataatacttttatattaggttaaggggtgattaatattaaaaaatatattttattattattgttttctataagctgtttttctttttcagcagtaaataatatatataaagaaagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn Intra-Species Hit: Count: 1 Min: 1 Max: 179 Len: 179 Subject: NC_003454_FN0499_FN0500|Hemin receptor:hypothetical protein|NEGATIVE:POSITIVE|[1148495,1149082]|588 HSP 1 e-value: 5.0E-28 bit: 127.0 Len: 64 Query Start:116 Query End:179 Subject Strand: POSITIVE Subject Start: 116 Subject End: 179 ...................................................................................................................gttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatg......................................................................................................................................................................................................................................................................................................................................................................................................................... ...................................................................................................................gttctaaatcaatactataaaagaatatttgtcccattactttaaattttcaagaaaaaatatg......................................................................................................................................................................................................................................................................................................................................................................................................................... HSP 2 e-value: 1.0E-16 bit: 89.7 Len: 66 Query Start:1 Query End:66 Subject Strand: POSITIVE Subject Start: 1 Subject End: 66 gactaataattttttcattattatttctcctttcaaannnnnnnaatttcaatcaatagtaaagag.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... gactaataattttttcattattatttctcctttcaaatttttttaatttcaatcaatagtaaagag.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACUAAUAAUUUUUUCAUUAUUAUUUCUCCUUUCAAAUUUUUUUAAUUUCAAUCAAUAGUAAAGAGAAUUAUUUUAUUUGUCAAUAUAUUUUUUUAGGAAUUUUAAUAUUUUUAUGUUCUAAAUCAAUACUAUAAAAGAAUAUUUGUCCCAUUACUUUAAAUUUUCAAGAAAAAAUAUG (((.(((((..(((((.((((((((......((.(((((.....))))).))..)))))))).))))).....))))).)))..(((((((((((.(((((..(((.....))).))))).................(((.(((((...........))))).)))..))))))))))) (-20.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUAUUUUUUCUUGAAAAUUUAAAGUAAUGGGACAAAUAUUCUUUUAUAGUAUUGAUUUAGAACAUAAAAAUAUUAAAAUUCCUAAAAAAAUAUAUUGACAAAUAAAAUAAUUCUCUUUACUAUUGAUUGAAAUUAAAAAAAUUUGAAAGGAGAAAUAAUAAUGAAAAAAUUAUUAGUC ..(((((((((((..((((((..(((((.((((..............(((((((..((((.....))))))))))).................((((....)))).......)))).))))).(((((....)))))..))))))..))))))))))).((((((.....))))))... (-21.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 615 70 21 161 112 NC_003454:FN0499|5end_Hemin_receptor_1148404..1148634_NEGATIVE 245 60 11 180 138 NC_003454:FN1985|5end_Inner_membrane_protein_488637..488867_NEGATIVE 242 40 4 179 139 NC_003454:FN0767|5end_Hemin-binding_periplasmic_protein_hmuT_precursor_1422630..1422860_POSITIVE 242 31 3 141 115 NC_003454:FN0024|5end_Hypothetical_exported_24-amino_acid_repeat_protein_663051..663281_POSITIVE 237 60 12 231 176 NC_003454:FN0772|5end_Flavodoxin_1428694..1428924_POSITIVE 233 53 11 128 84 NC_003454:FN0723|5end_hypothetical_protein_1381528..1381758_POSITIVE 233 31 3 142 115 NC_003454:FN2121|5end_Hypothetical_exported_24-amino_acid_repeat_protein_629728..629958_NEGATIVE 229 45 7 115 80 NC_003454:FN0222|5end_Melibiose_carrier_protein_846812..847042_POSITIVE 229 48 1 122 76 NC_003454:FN1725|5end_Potassium_uptake_protein_KtrB_224671..224901_NEGATIVE 228 50 8 200 158 NC_003454:FN1495|5end_hypothetical_protein_2174410..2174640_NEGATIVE 228 50 8 224 182 NC_003454:FN1494|5end_Rod_shape-determining_protein_mreC_2174386..2174616_NEGATIVE 228 50 8 123 81 NC_003454:FN1493|5end_hypothetical_protein_2174063..2174293_NEGATIVE 228 58 13 106 62 NC_003454:FN0474|5end_Acriflavin_resistance_protein_B_1118939..1119169_POSITIVE 221 56 11 126 84 NC_003454:FN1968|5end_Hemin_transport_system_ATP-binding_protein_hmuV_467189..467419_NEGATIVE 218 118 71 165 114 NC_003454:FN1063|5end_N-acyl-L-amino_acid_amidohydrolase_1709325..1709555_POSITIVE 216 44 3 101 64 NC_003454:FN1408|5end_Aminoacyl-histidine_dipeptidase_2067395..2067625_POSITIVE 210 38 4 110 77 NC_003454:FN0280|5end_hypothetical_protein_907752..907982_NEGATIVE 210 32 3 136 100 NC_003454:FN1792|5end_hypothetical_protein_273264..273494_NEGATIVE 210 54 11 120 84 NC_003454:FN1754|5end_thiazole_synthase_251117..251347_NEGATIVE 209 52 11 91 48 NC_003454:FN1163|5end_Thioredoxin_reductase_1820771..1821001_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 233 53 11 124 80 NC_003454:FN0722|3end_WD-repeat_family_protein_1381604..1381754_POSITIVE 233 31 3 72 45 NC_003454:FN2122|3end_Phenylalanyl-tRNA_synthetase_beta_chain_629798..629948_NEGATIVE 229 48 1 107 61 NC_003454:FN1726|3end_Na+_driven_multidrug_efflux_pump_224686..224836_NEGATIVE 228 50 8 123 81 NC_003454:FN1494|3end_Rod_shape-determining_protein_mreC_2174063..2174213_NEGATIVE 228 50 8 123 81 NC_003454:FN1496|3end_Rod_shape-determining_protein_mreC_-13..137_NEGATIVE 221 56 11 129 87 NC_003454:FN1969|3end_Hemin_transport_system_permease_protein_hmuU_467186..467336_NEGATIVE 210 38 4 97 64 NC_003454:FN0281|3end_DNA_polymerase_III_alpha_subunit_907765..907915_NEGATIVE 209 52 11 104 61 NC_003454:FN1162|3end_Hydroxyacylglutathione_hydrolase_1820864..1821014_POSITIVE 208 52 11 125 77 NC_003454:FN1189|3end_hypothetical_protein_1851750..1851900_POSITIVE 208 44 6 101 63 NC_003454:FN1034|3end_Transcriptional_regulator,_TetR_family_1688345..1688495_POSITIVE 207 58 15 130 88 NC_003454:FN0345|3end_Permease_977761..977911_NEGATIVE 206 70 26 132 83 NC_003454:FN1190|3end_Probable_cadmium-transporting_ATPase_1853964..1854114_POSITIVE 203 33 2 103 69 NC_003454:FN0583|3end_hypothetical_protein_1239518..1239668_POSITIVE 203 104 62 65 19 NC_003454:FN1846|3end_hypothetical_protein_336225..336375_POSITIVE 199 102 67 99 64 NC_003454:FN1802|3end_hypothetical_protein_284186..284336_POSITIVE 198 60 12 95 41 NC_003454:FN0072|3end_2-amino-4-hydroxy-6-_hydroxymethyldihydropteridine_pyrophosphokinase_712619..712769_POSITIVE 198 149 107 65 26 NC_003454:FN2016|3end_ATP-dependent_Clp_protease_proteolytic_subunit_518267..518417_NEGATIVE 198 50 9 59 19 NC_003454:FN1940|3end_Na+_driven_multidrug_efflux_pump_439585..439735_NEGATIVE 197 31 3 76 45 NC_003454:FN2118|3end_Hypothetical_exported_24-amino_acid_repeat_protein_626287..626437_NEGATIVE 194 40 4 70 36 NC_003454:FN1911|3end_Outer_membrane_protein_403249..403399_NEGATIVE