Origin IGS: tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttatacctcatttcttaaataatactagcatacttattctttcttgataattttagaataatttgtactgttattgtaccatattttttcttaattgtcaagtttccaactaggaataaagtgtttataagaattcttactcatacatatttagtttctaaaattcctagttattgtattttaaaatttgtaaaattaaaattctgaatattctaaca Mask Tandem Repeat Region ================================================ tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Find is-nt database================================================ Query_seq: FN0443:FN0444|FN0443:FN0444:hypothetical protein:Phosphoadenosine phosphosulfate reductase:->->:1082994..1083213 220 tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0443:FN0444|FN0443:FN0444:hypothetical protein:Phosphoadenosine phosphosulfate reductase:->->:1082994..1083213 220 tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0443:FN0444|FN0443:FN0444:hypothetical protein:Phosphoadenosine phosphosulfate reductase:->->:1082994..1083213 220 tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: + Start: 85 End: 201 .................................................................................... M L I N T L F L V G N L T I K K K Y G T I T V Q I I L K L S R K N K Y A S I I ................... Protein_Len: 60 Strand: - Start: 15 End: 200 .............. L K V F K L I C Y S P I K S V L Y T H T L I R I F V K N R T P F K V I L F F Y P V I V T C I I R F N D L F F L Y A L I M .................... tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 91 Start: 117 End: 146 ....................................................................................................................CTTGACAATTAAGAAAAAATATGGTACAAT.......................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0443:FN0444|FN0443:FN0444:hypothetical protein:Phosphoadenosine phosphosulfate reductase:->->:1082994..1083213 220 tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Intra-Species Hit: Count: 1 Min: 1 Max: 220 Len: 220 Subject: NC_003454_FN0443_FN0444|hypothetical protein:Phosphoadenosine phosphosulfate reductase|POSITIVE:POSITIVE|[1082994,1083213]|220 HSP 1 e-value: 1.0E-121 bit: 436.0 Len: 220 Query Start:1 Query End:220 Subject Strand: POSITIVE Subject Start: 1 Subject End: 220 tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa tgttagaatattcagaattttaattttacaaattttaaaatacaataactaggaattttagaaactaaatatgtatgagtaagaattcttataaacactttattcctagttggaaacttgacaattaagaaaaaatatggtacaataacagtacaaattattctaaaattatcaagaaagaataagtatgctagtattatttaagaaatgaggtataaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUUAGAAUAUUCAGAAUUUUAAUUUUACAAAUUUUAAAAUACAAUAACUAGGAAUUUUAGAAACUAAAUAUGUAUGAGUAAGAAUUCUUAUAAACACUUUAUUCCUAGUUGGAAACUUGACAAUUAAGAAAAAAUAUGGUACAAUAACAGUACAAAUUAUUCUAAAAUUAUCAAGAAAGAAUAAGUAUGCUAGUAUUAUUUAAGAAAUGAGGUAUAAAA ........(((((.(.(((((((...........))))))).)..(((((((((((...((..((.......))....(((((....))))).....))..)))))))))))....(((((.((((.((((........((((.......))))......))))..)))).)))))...)))))..((((((..((((........)))).))))))... (-33.44) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAUACCUCAUUUCUUAAAUAAUACUAGCAUACUUAUUCUUUCUUGAUAAUUUUAGAAUAAUUUGUACUGUUAUUGUACCAUAUUUUUUCUUAAUUGUCAAGUUUCCAACUAGGAAUAAAGUGUUUAUAAGAAUUCUUACUCAUACAUAUUUAGUUUCUAAAAUUCCUAGUUAUUGUAUUUUAAAAUUUGUAAAAUUAAAAUUCUGAAUAUUCUAACA (((((.....((..(.(((((..((((.................((((((((((..((((.(((..((((.......))))...)))..)))).)))))))))).....((((((((((...((((...((((....))))...))))...(((((...)))))))))))))))...))))))))).)..))......)))))................. (-33.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 221 90 49 47 6 NC_003454:FN1408|5end_Aminoacyl-histidine_dipeptidase_2067395..2067625_POSITIVE 218 113 71 76 42 NC_003454:FN1931|5end_Protease_432421..432651_NEGATIVE 200 119 71 226 173 NC_003454:FN1970|5end_Hemin-binding_periplasmic_protein_hmuT_precursor_469056..469286_NEGATIVE 194 198 161 154 113 NC_003454:FN0838|5end_hypothetical_protein_1500747..1500977_POSITIVE 189 119 71 145 86 NC_003454:FN0815|5end_Propionate_permease_1474390..1474620_POSITIVE 183 215 172 45 3 NC_003454:FN0184|5end_hypothetical_protein_809087..809317_NEGATIVE 183 127 92 162 123 NC_003454:FN1607|5end_Cytidylate_kinase_117893..118123_NEGATIVE 182 145 104 158 124 NC_003454:FN2105|5end_Integral_membrane_protein_614701..614931_POSITIVE 181 115 73 121 82 NC_003454:FN1308|5end_Phosphatidate_cytidylyltransferase_1964140..1964370_POSITIVE 177 214 175 51 8 NC_003454:FN0145|5end_hypothetical_protein_774306..774536_POSITIVE 174 116 72 124 71 NC_003454:FN1537|5end_Arsenical_pump-driving_ATPase_51112..51342_NEGATIVE 171 215 171 196 154 NC_003454:FN0454|5end_Aldehyde_dehydrogenase_B_1099467..1099697_POSITIVE 166 108 64 187 140 NC_003454:FN0205|5end_Sodium/glutamate_symport_carrier_protein_831469..831699_POSITIVE 165 90 51 85 47 NC_003454:FN1954|5end_Glycerophosphoryl_diester_phosphodiesterase_459992..460222_NEGATIVE 164 116 77 226 184 NC_003454:FN0254|5end_Fusobacterium_outer_membrane_protein_family_876277..876507_POSITIVE 163 90 47 57 16 NC_003454:FN1014|5end_Dihydrofolate_synthase_1669601..1669831_NEGATIVE 162 157 115 58 20 NC_003454:FN2077|5end_NA+/H+_antiporter_NHAC_587900..588130_POSITIVE 161 120 98 46 23 NC_003454:FN1911|5end_Outer_membrane_protein_405285..405515_NEGATIVE 161 127 86 87 40 NC_003454:FN1703|5end_ADP-L-glycero-D-manno-heptose-6-epimerase_198992..199222_NEGATIVE 158 118 71 46 4 NC_003454:FN1212|5end_hypothetical_protein_1873168..1873398_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 218 113 71 79 45 NC_003454:FN1932|3end_Dephospho-CoA_kinase_432418..432568_NEGATIVE 202 193 162 42 11 NC_003454:FN0197|3end_Methyltransferase_823544..823694_POSITIVE 200 119 71 149 96 NC_003454:FN1971|3end_Hemin_receptor_469133..469283_NEGATIVE 183 127 92 146 107 NC_003454:FN1608|3end_Ribosomal_protein_L11_methyltransferase_117909..118059_NEGATIVE 182 145 104 137 103 NC_003454:FN2104|3end_hypothetical_protein_614760..614910_POSITIVE 179 127 85 66 27 NC_003454:FN0814|3end_Propionate_CoA-transferase_1474383..1474533_POSITIVE 174 116 72 127 74 NC_003454:FN1538|3end_Arsenical_pump-driving_ATPase_51109..51259_NEGATIVE 166 130 97 149 125 NC_003454:FN0177|3end_Cell_division_inhibitor_MinE_802336..802486_POSITIVE 163 90 47 57 16 NC_003454:FN1015|3end_5'-methylthioadenosine_nucleosidase_1669601..1669751_NEGATIVE 161 117 73 105 51 NC_003454:FN1953|3end_Na(+)-linked_D-alanine_glycine_permease_459197..459347_NEGATIVE 160 108 72 109 74 NC_003454:FN0589|3end_Transcriptional_regulator,_MarR_family_1242833..1242983_POSITIVE 159 155 111 59 2 NC_003454:FN0615|3end_Export_ABC_transporter_1274991..1275141_POSITIVE 158 100 51 57 16 NC_003454:FN1154|3end_Ribonuclease_BN_1809502..1809652_POSITIVE 156 130 81 149 107 NC_003454:FN1810|3end_Manganese_transport_system_membrane_protein_mntB_291060..291210_NEGATIVE 155 120 71 116 72 NC_003454:FN1360|3end_Dipeptide_transport_system_permease_protein_dppB_2013131..2013281_POSITIVE 155 80 37 67 28 NC_003454:FN1738|3end_ATP_synthase_subunit_C_236340..236490_NEGATIVE 154 119 71 151 110 NC_003454:FN1906|3end_Cytosol_aminopeptidase_399883..400033_POSITIVE 151 121 83 147 108 NC_003454:FN1027|3end_Rod_shape-determining_protein_rodA_1680823..1680973_NEGATIVE 150 88 50 119 83 NC_003454:FN0314|3end_Caffeoyl-CoA_O-methyltransferase_951931..952081_POSITIVE 149 114 76 58 15 NC_003454:FN1282|3end_LSU_ribosomal_protein_L17P_1941269..1941419_NEGATIVE