Origin IGS: atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tatctcactccttattttaagttacttaataatttaaatttaccaattaaaatcttttcacttatactttaccatattttttctattttttaaatggatatttttcaattttcttaaattgcttgaatttgtatcaaaaaagataataaaactat Mask Tandem Repeat Region ================================================ atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Find is-nt database================================================ Query_seq: FN0321:FN0322|FN0321:FN0322:heat shock protein 90:fructose-bisphosphate aldolase:->->:959598..959752 155 atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0321:FN0322|FN0321:FN0322:heat shock protein 90:fructose-bisphosphate aldolase:->->:959598..959752 155 atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0321:FN0322|FN0321:FN0322:heat shock protein 90:fructose-bisphosphate aldolase:->->:959598..959752 155 atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: - Start: 2 End: 118 . L K I I K K S V F E L L K L F N F F I W K F F L F F I T F Y L H F S K L Q Y M ..................................... atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 88 End: 117 .......................................................................................TTTACCAATTAAAATCTTTTCACTTATACT...................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0321:FN0322|FN0321:FN0322:heat shock protein 90:fructose-bisphosphate aldolase:->->:959598..959752 155 atagttttattatcttttttgatacaaattcaagcaatttaagaaaattgaaaaatatccatttaaaaaatagaaaaaatatggtaaagtataagtgaaaagattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata Intra-Species Hit: Count: 1 Min: 1 Max: 155 Len: 155 Subject: NC_003454_FN0321_FN0322|heat shock protein 90:fructose-bisphosphate aldolase|POSITIVE:POSITIVE|[959598,959752]|155 HSP 1 e-value: 1.0E-22 bit: 107.0 Len: 54 Query Start:102 Query End:155 Subject Strand: POSITIVE Subject Start: 102 Subject End: 155 .....................................................................................................gattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata .....................................................................................................gattttaattggtaaatttaaattattaagtaacttaaaataaggagtgagata HSP 2 e-value: 2.0E-15 bit: 83.8 Len: 42 Query Start:1 Query End:42 Subject Strand: POSITIVE Subject Start: 1 Subject End: 42 atagttttattatcttttttgatacaaattcaagcaatttaa................................................................................................................. atagttttattatcttttttgatacaaattcaagcaatttaa................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGUUUUAUUAUCUUUUUUGAUACAAAUUCAAGCAAUUUAAGAAAAUUGAAAAAUAUCCAUUUAAAAAAUAGAAAAAAUAUGGUAAAGUAUAAGUGAAAAGAUUUUAAUUGGUAAAUUUAAAUUAUUAAGUAACUUAAAAUAAGGAGUGAGAUA ...((((((((.(((((((..((((.........((((((....))))))........((((..................))))....))))....)))))))..(((.(((((((.......))))))).)))............)))))))). (-16.07) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUCUCACUCCUUAUUUUAAGUUACUUAAUAAUUUAAAUUUACCAAUUAAAAUCUUUUCACUUAUACUUUACCAUAUUUUUUCUAUUUUUUAAAUGGAUAUUUUUCAAUUUUCUUAAAUUGCUUGAAUUUGUAUCAAAAAAGAUAAUAAAACUAU ...............(((((((((.....))))))))).............(((((((.....((((..............((((((.....)))))).((((..((((((....))))))...))))..))))...)))))))........... (-12.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 193 121 82 77 38 NC_003454:FN1964|5end_O-linked_GLCNAC_transferase_463775..464005_NEGATIVE 189 96 56 62 26 NC_003454:FN1502|5end_Nickel_transport_system_permease_protein_nikC_7522..7752_NEGATIVE 172 49 1 164 113 NC_003454:FN1926|5end_Nitrogen_regulatory_IIA_protein_425287..425517_NEGATIVE 167 109 87 37 16 NC_003454:FN1995|5end_hypothetical_protein_498245..498475_POSITIVE 163 117 79 193 152 NC_003454:FN1435|5end_Export_ABC_transporter_2105034..2105264_POSITIVE 163 35 2 40 16 NC_003454:FN1413|5end_translation_initiation_factor_IF-2B_subunit_alpha_2074270..2074500_NEGATIVE 159 98 58 132 87 NC_003454:FN1036|5end_hypothetical_protein_1688760..1688990_POSITIVE 159 65 24 191 146 NC_003454:FN1987|5end_Transcriptional_regulator,_GntR_family_490668..490898_NEGATIVE 154 108 70 227 188 NC_003454:FN1655|5end_Hypothetical_cytosolic_protein_150898..151128_POSITIVE 149 108 74 139 104 NC_003454:FN0355|5end_S-adenosylmethionine_synthetase_987227..987457_NEGATIVE 147 130 81 168 120 NC_003454:FN1335|5end_Protein_translocase_subunit_YajC_1989011..1989241_NEGATIVE 147 39 1 53 15 NC_003454:FN0494|5end_Short_chain_dehydrogenase_1138777..1139007_POSITIVE 146 45 1 201 162 NC_003454:FN0360|5end_ATP_synthase_subunit_A_992356..992586_NEGATIVE 145 103 70 183 147 NC_003454:FN0935|5end_hypothetical_protein_1588526..1588756_NEGATIVE 143 67 33 98 62 NC_003454:FN0471|5end_Cell_surface_protein_1116525..1116755_POSITIVE 141 128 82 231 188 NC_003454:FN1952|5end_Na(+)-linked_D-alanine_glycine_permease_459050..459280_NEGATIVE 137 45 10 179 146 NC_003454:FN2109|5end_UDP-glucose_4-epimerase_620848..621078_POSITIVE 137 107 71 200 167 NC_003454:FN1975|5end_ATP-dependent_RNA_helicase_475599..475829_POSITIVE 136 80 38 191 149 NC_003454:FN0923|5end_Cardiolipin_synthetase_1578977..1579207_NEGATIVE 136 81 44 75 38 NC_003454:FN0713|5end_Chromate_transport_protein_1371333..1371563_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 189 96 56 81 45 NC_003454:FN1503|3end_Nickel_transport_system_permease_protein_nikB_7503..7653_NEGATIVE 165 60 15 68 22 NC_003454:FN1935|3end_Adenine-specific_methyltransferase_436060..436210_NEGATIVE 159 98 58 131 86 NC_003454:FN1035|3end_Iron-sulfur_flavoprotein_1688839..1688989_POSITIVE 152 129 82 85 41 NC_003454:FN1953|3end_Na(+)-linked_D-alanine_glycine_permease_459197..459347_NEGATIVE 148 116 85 150 121 NC_003454:FN0216|3end_3-oxoacyl-[acyl-carrier_protein]_reductase_840112..840262_NEGATIVE 147 78 34 71 20 NC_003454:FN1927|3end_DEGV_protein_425415..425565_NEGATIVE 145 103 70 60 24 NC_003454:FN0936|3end_CapC_protein_1588649..1588799_NEGATIVE 138 149 116 79 52 NC_003454:FN1281|3end_Cysteine_protease_1941242..1941392_POSITIVE 136 46 4 55 12 NC_003454:FN1468|3end_Flavoredoxin_2149792..2149942_NEGATIVE 136 147 102 108 64 NC_003454:FN1442|3end_Transposase_2110155..2110305_NEGATIVE 136 147 102 108 64 NC_003454:FN0841|3end_Transposase_1502804..1502954_POSITIVE 136 147 102 108 64 NC_003454:FN0787|3end_Transposase_1443908..1444058_POSITIVE 136 81 44 78 41 NC_003454:FN0712|3end_Chromate_transport_protein_1371416..1371566_POSITIVE 136 44 5 146 102 NC_003454:FN0489|3end_Prolipoprotein_diacylglyceryl_transferase_1134559..1134709_NEGATIVE 136 147 102 108 64 NC_003454:FN0486|3end_Transposase_1131710..1131860_POSITIVE 136 147 102 108 64 NC_003454:FN0139|3end_Transposase_771640..771790_POSITIVE 136 114 83 44 15 NC_003454:FN0103|3end_Ribonucleoside-diphosphate_reductase_beta_chain_739607..739757_POSITIVE 135 40 5 143 103 NC_003454:FN1225|3end_UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--meso-_lanthionine_ligase_1883366..1883516_NEGATIVE 134 53 15 50 22 NC_003454:FN1659|3end_hypothetical_protein_155826..155976_POSITIVE 132 45 3 90 33 NC_003454:FN0920|3end_Protease_HTPX_1574618..1574768_NEGATIVE