Origin IGS: tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 attgcctcacgcctttctaattctattttaaattttgtaatttattttctataaaaaaatttttatatcttcaatcattgtagcactttttaaaaaaaataacaatatatagtatatatacttttaaagtaccactatatattgttattttaaaatatcgttaatatgggatttataaaattttaaaataaaaatagagttattatataaaaaaattttaataactctatcttaattttatattttttattaaaaata Mask Tandem Repeat Region ================================================ tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Find is-nt database================================================ Query_seq: FN0310:FN0311|FN0310:FN0311:Iron(III)-transport ATP-binding protein sfuC:anaerobic ribonucleoside triphosphate reductase:->->:947007..947264 258 tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0310:FN0311|FN0310:FN0311:Iron(III)-transport ATP-binding protein sfuC:anaerobic ribonucleoside triphosphate reductase:->->:947007..947264 258 tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0310:FN0311|FN0310:FN0311:Iron(III)-transport ATP-binding protein sfuC:anaerobic ribonucleoside triphosphate reductase:->->:947007..947264 258 tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Predict ORF larger than 30AA ================================================ tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 153 End: 182 ........................................................................................................................................................ATTGTTATTTTTTTTAAAAAGTGCTACAAT............................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 65 HP_score: -3.1 Tail_Score: -5.46706 Start: 30 End: 64 Full_Region: AATATAAAATTAAGA TAGAGTTATTAAA ATTTTTTTA TATAATAACTCTA TTTTTATTTTAAAAT .............................TAGAGTTATTAAAATTTTTTTATATAATAACTCTA.................................................................................................................................................................................................. Find igs database================================================ Query_seq: FN0310:FN0311|FN0310:FN0311:Iron(III)-transport ATP-binding protein sfuC:anaerobic ribonucleoside triphosphate reductase:->->:947007..947264 258 tatttttaataaaaaatataaaattaagatagagttattaaaatttttttatataataactctatttttattttaaaattttataaatcccatattaacgatattttaaaataacaatatatagtggtactttaaaagtatatatactatatattgttattttttttaaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat Intra-Species Hit: Count: 1 Min: 1 Max: 258 Len: 258 Subject: NC_003454_FN0310_FN0311|Iron(III)-transport ATP-binding protein sfuC:anaerobic ribonucleoside triphosphate reductase|POSITIVE:POSITIVE|[947007,947264]|258 HSP 1 e-value: 5.0E-32 bit: 139.0 Len: 91 Query Start:168 Query End:258 Subject Strand: POSITIVE Subject Start: 168 Subject End: 258 .......................................................................................................................................................................aaaaagtgctacaatgattgaagatataaaaannnnnnnatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat .......................................................................................................................................................................aaaaagtgctacaatgattgaagatataaaaatttttttatagaaaataaattacaaaatttaaaatagaattagaaaggcgtgaggcaat HSP 2 e-value: 2.0E-10 bit: 67.9 Len: 34 Query Start:1 Query End:34 Subject Strand: POSITIVE Subject Start: 1 Subject End: 34 tatttttaataaaaaatataaaattaagatagag................................................................................................................................................................................................................................ tatttttaataaaaaatataaaattaagatagag................................................................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUUUUUAAUAAAAAAUAUAAAAUUAAGAUAGAGUUAUUAAAAUUUUUUUAUAUAAUAACUCUAUUUUUAUUUUAAAAUUUUAUAAAUCCCAUAUUAACGAUAUUUUAAAAUAACAAUAUAUAGUGGUACUUUAAAAGUAUAUAUACUAUAUAUUGUUAUUUUUUUUAAAAAGUGCUACAAUGAUUGAAGAUAUAAAAAUUUUUUUAUAGAAAAUAAAUUACAAAAUUUAAAAUAGAAUUAGAAAGGCGUGAGGCAAU (((((((.((((((((..((((((.(((((((((((((((.............))))))))))))))).))))))..(((((...((((........((........((((((((((((((((((((((((.....)))))...))))))))))))))))))).........))........)))).)))))........)))))))).))))))).............................(.(....).)... (-53.34) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGCCUCACGCCUUUCUAAUUCUAUUUUAAAUUUUGUAAUUUAUUUUCUAUAAAAAAAUUUUUAUAUCUUCAAUCAUUGUAGCACUUUUUAAAAAAAAUAACAAUAUAUAGUAUAUAUACUUUUAAAGUACCACUAUAUAUUGUUAUUUUAAAAUAUCGUUAAUAUGGGAUUUAUAAAAUUUUAAAAUAAAAAUAGAGUUAUUAUAUAAAAAAAUUUUAAUAACUCUAUCUUAAUUUUAUAUUUUUUAUUAAAAAUA ..........................................((((((..((((((((..(((((((...((...(((..((((...(((((((....(((((((((((((((.....((((.....))))..))))))))))))))))))))))....))))..))).))..)))))))...((((((.((.((((((((((((.............)))))))))))).)).))))))..))))))))..)))))) (-44.12) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 212 149 102 95 50 NC_003454:FN1347|5end_Hypothetical_cytosolic_protein_2000300..2000530_NEGATIVE 198 70 26 47 4 NC_003454:FN1746|5end_Cystathionine_beta-lyase_244341..244571_POSITIVE 192 50 2 189 134 NC_003454:FN1033|5end_Methyltransferase_1687545..1687775_NEGATIVE 191 107 71 140 111 NC_003454:FN0523|5end_Exonuclease_SBCD_1176887..1177117_NEGATIVE 187 75 32 147 102 NC_003454:FN1278|5end_Acetyltransferase_1937174..1937404_NEGATIVE 183 179 141 82 43 NC_003454:FN1118|5end_hypothetical_protein_1760559..1760789_POSITIVE 182 80 32 133 85 NC_003454:FN1255|5end_NagD_protein_1911892..1912122_NEGATIVE 178 186 141 177 129 NC_003454:FN1802|5end_hypothetical_protein_283948..284178_POSITIVE 172 198 152 124 74 NC_003454:FN1545|5end_Ferredoxin_like_protein_59133..59363_POSITIVE 171 166 127 32 1 NC_003454:FN1643|5end_LSU_ribosomal_protein_L23P_140017..140247_NEGATIVE 170 50 3 183 137 NC_003454:FN0326|5end_LSU_ribosomal_protein_L35P_961505..961735_NEGATIVE 169 164 125 95 62 NC_003454:FN0970|5end_Precorrin-8X_methylmutase_1623266..1623496_NEGATIVE 168 217 173 177 133 NC_003454:FN1403|5end_Histidine_permease_2061743..2061973_POSITIVE 166 67 25 190 152 NC_003454:FN1412|5end_5-methylthioribose_kinase_2073206..2073436_NEGATIVE 164 200 152 187 145 NC_003454:FN0566|5end_Hypothetical_exported_24-amino_acid_repeat_protein_1218482..1218712_NEGATIVE 164 64 23 131 90 NC_003454:FN1647|5end_hypothetical_protein_141983..142213_POSITIVE 162 99 59 180 140 NC_003454:FN1216|5end_RRF2_family_protein_1876602..1876832_NEGATIVE 162 189 144 180 141 NC_003454:FN1526|5end_Fusobacterium_outer_membrane_protein_family_38183..38413_NEGATIVE 160 247 220 165 136 NC_003454:FN0810|5end_Low-specificity_threonine_aldolase_1469038..1469268_POSITIVE 158 90 41 221 171 NC_003454:FN0136|5end_Transposase_770506..770736_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 214 68 21 65 18 NC_003454:FN0310|3end_Iron(III)-transport_ATP-binding_protein_sfuC_946946..947096_POSITIVE 209 70 21 93 40 NC_003454:FN0586|3end_Two-component_sensor_kinase_czcS_1241857..1242007_POSITIVE 191 107 71 143 114 NC_003454:FN0524|3end_DNA_helicase_II_1176884..1177034_NEGATIVE 187 70 25 79 24 NC_003454:FN1007|3end_hypothetical_protein_1662482..1662632_POSITIVE 183 179 141 79 40 NC_003454:FN1117|3end_LSU_ribosomal_protein_L21P_1760636..1760786_POSITIVE 182 80 32 105 57 NC_003454:FN1256|3end_C4-dicarboxylate_transporter_large_subunit_1911920..1912070_NEGATIVE 177 80 31 100 51 NC_003454:FN0882|3end_Hemin_transport_system_ATP-binding_protein_hmuV_1542947..1543097_NEGATIVE 172 198 152 121 71 NC_003454:FN1544|3end_Probable_electron_transfer_flavoprotein-quinone_oxidoreductase_ydiS_59210..59360_POSITIVE 171 166 127 32 1 NC_003454:FN1644|3end_50S_ribosomal_protein_L4_140017..140167_NEGATIVE 170 111 71 59 7 NC_003454:FN0834|3end_hypothetical_protein_1498088..1498238_POSITIVE 170 50 3 105 59 NC_003454:FN0327|3end_Bacterial_Protein_Translation_Initiation_Factor_3_(IF-3)_961583..961733_NEGATIVE 169 164 125 80 47 NC_003454:FN0971|3end_Huntington_interacting_Protein_HYPE_1623281..1623431_NEGATIVE 168 217 173 54 10 NC_003454:FN1402|3end_Hypothetical_membrane-spanning_protein_2061700..2061850_POSITIVE 165 75 31 98 56 NC_003454:FN1132|3end_Nicotinamide-nucleotide_adenylyltransferase_1780254..1780404_POSITIVE 157 69 21 84 35 NC_003454:FN0761|3end_Bvg_accessory_factor_1419719..1419869_POSITIVE 156 70 22 93 40 NC_003454:FN1216|3end_RRF2_family_protein_1876171..1876321_NEGATIVE 156 180 145 83 46 NC_003454:FN0460|3end_delta-aminolevulinic_acid_dehydratase_1106530..1106680_POSITIVE 153 99 59 85 40 NC_003454:FN0648|3end_Exoenzyme_regulatory_protein_aepA_precursor_1304532..1304682_NEGATIVE 153 107 63 143 99 NC_003454:FN2004|3end_Cobalt_transport_ATP-binding_protein_cbiO_505177..505327_POSITIVE 153 93 58 74 37 NC_003454:FN1901|3end_Transcription_regulator,_CRP_family_389364..389514_NEGATIVE