Origin IGS: tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaactcctccatatatcttaataattaaaattatttttccttaattattttgtaacataaagatacccctaggaagacatgcatatactataaatcttagtgctgcttccttccagatctgacacggttcgacaataatatatcatatcccctaagggcaatatatattatcatttttattcttttttgtcaactttata Mask Tandem Repeat Region ================================================ tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Find is-nt database================================================ Query_seq: FN0173:FN0174|FN0173:FN0174:hypothetical protein:Enoyl-[acyl-carrier-protein] reductase:->->:799365..799566 202 tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0173:FN0174|FN0173:FN0174:hypothetical protein:Enoyl-[acyl-carrier-protein] reductase:->->:799365..799566 202 tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0173:FN0174|FN0173:FN0174:hypothetical protein:Enoyl-[acyl-carrier-protein] reductase:->->:799365..799566 202 tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 37 End: 129 .................................... M L P L G D M I Y Y C R T V S D L E G S S T K I Y S I C M S S ......................................................................... Protein_Len: 32 Strand: + Start: 62 End: 157 ............................................................. M I V E P C Q I W K E A A L R F I V Y A C L P R G I F M L Q N N ............................................. tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 86 Start: 7 End: 36 ......GTTGACAAAAAAGAATAAAAATGATAATAT...................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0173:FN0174|FN0173:FN0174:hypothetical protein:Enoyl-[acyl-carrier-protein] reductase:->->:799365..799566 202 tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Intra-Species Hit: Count: 1 Min: 1 Max: 202 Len: 202 Subject: NC_003454_FN0173_FN0174|hypothetical protein:Enoyl-[acyl-carrier-protein] reductase|POSITIVE:POSITIVE|[799365,799566]|202 HSP 1 e-value: 1.0E-111 bit: 400.0 Len: 202 Query Start:1 Query End:202 Subject Strand: POSITIVE Subject Start: 1 Subject End: 202 tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta tataaagttgacaaaaaagaataaaaatgataatatatattgcccttaggggatatgatatattattgtcgaaccgtgtcagatctggaaggaagcagcactaagatttatagtatatgcatgtcttcctaggggtatctttatgttacaaaataattaaggaaaaataattttaattattaagatatatggaggagtttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAUAAAGUUGACAAAAAAGAAUAAAAAUGAUAAUAUAUAUUGCCCUUAGGGGAUAUGAUAUAUUAUUGUCGAACCGUGUCAGAUCUGGAAGGAAGCAGCACUAAGAUUUAUAGUAUAUGCAUGUCUUCCUAGGGGUAUCUUUAUGUUACAAAAUAAUUAAGGAAAAAUAAUUUUAAUUAUUAAGAUAUAUGGAGGAGUUUUA .........(((............................((((((((((((((((((((((((((.(((.....((((....(((....)))....))))...)))..))))))))).)))))).))))))))))).((((((((.....(((((((((((........))))))))))).....))))))))..)))... (-47.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAACUCCUCCAUAUAUCUUAAUAAUUAAAAUUAUUUUUCCUUAAUUAUUUUGUAACAUAAAGAUACCCCUAGGAAGACAUGCAUAUACUAUAAAUCUUAGUGCUGCUUCCUUCCAGAUCUGACACGGUUCGACAAUAAUAUAUCAUAUCCCCUAAGGGCAAUAUAUAUUAUCAUUUUUAUUCUUUUUUGUCAACUUUAUA .............((((....(((((((((............))))))))).))))......((((......((((((.((.((((...............)))))))))))).....)))).....((((.(((((((((((((.....(((....)))....)))))))))................))))))))..... (-29.15) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 258 70 26 226 187 NC_003454:FN1670|5end_Choline_kinase_168266..168496_NEGATIVE 232 69 27 130 95 NC_003454:FN0033|5end_hypothetical_protein_674969..675199_NEGATIVE 222 155 113 58 18 NC_003454:FN1268|5end_Methionyl-tRNA_synthetase_1925593..1925823_NEGATIVE 219 148 111 196 160 NC_003454:FN0079|5end_Ethanolamine_ammonia-lyase_heavy_chain_717662..717892_POSITIVE 218 198 166 207 178 NC_003454:FN1412|5end_5-methylthioribose_kinase_2073206..2073436_NEGATIVE 217 100 66 229 197 NC_003454:FN0438|5end_Branched_chain_amino_acid_transport_system_II_carrier_protein_1080822..1081052_NEGATIVE 214 141 101 229 183 NC_003454:FN0182|5end_Sarcosine_oxidase_alpha_subunit_807066..807296_NEGATIVE 214 120 76 205 152 NC_003454:FN1873|5end_Bis(5'-nucleosyl)-tetraphosphatase_363440..363670_NEGATIVE 212 102 61 220 175 NC_003454:FN1449|5end_Fusobacterium_outer_membrane_protein_family_2128109..2128339_NEGATIVE 211 126 82 204 155 NC_003454:FN0094|5end_hypothetical_protein_729740..729970_NEGATIVE 208 146 104 38 2 NC_003454:FN1546|5end_translation_elongation_factor_EF-G_61601..61831_NEGATIVE 206 90 46 199 160 NC_003454:FN1288|5end_hypothetical_protein_1944580..1944810_POSITIVE 206 130 84 103 53 NC_003454:FN0006|5end_tRNA_modification_GTPase_644505..644735_POSITIVE 205 144 116 209 180 NC_003454:FN0608|5end_Exoribonuclease_II_1262142..1262372_POSITIVE 203 140 96 162 134 NC_003454:FN1992|5end_Ribose-phosphate_pyrophosphokinase_495965..496195_POSITIVE 203 130 84 157 117 NC_003454:FN1631|5end_SSU_ribosomal_protein_S14P_135247..135477_NEGATIVE 202 159 120 86 52 NC_003454:FN1605|5end_Adenylosuccinate_synthetase_115276..115506_NEGATIVE 201 130 92 62 27 NC_003454:FN1723|5end_glucose-inhibited_division_protein_A_222645..222875_NEGATIVE 199 81 42 137 105 NC_003454:FN0938|5end_hypothetical_protein_1591125..1591355_NEGATIVE 192 140 107 186 141 NC_003454:FN0279|5end_Lipoprotein_906479..906709_NEGATIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 249 150 110 63 25 NC_003454:FN0173|3end_hypothetical_protein_799304..799454_POSITIVE 215 136 99 150 106 NC_003454:FN1106|3end_L-serine_dehydratase_1751873..1752023_POSITIVE 206 130 84 97 47 NC_003454:FN0005|3end_Jag_protein_644579..644729_POSITIVE 203 130 84 136 96 NC_003454:FN1632|3end_LSU_ribosomal_protein_L5P_135268..135418_NEGATIVE 202 159 120 76 42 NC_003454:FN1606|3end_3-deoxy-D-manno-octulosonic-acid_transferase_115286..115436_NEGATIVE 201 130 92 53 18 NC_003454:FN1724|3end_Potassium_uptake_protein_KtrA_222654..222804_NEGATIVE 189 200 177 79 54 NC_003454:FN0995|3end_hypothetical_protein_1650672..1650822_POSITIVE 189 130 84 113 68 NC_003454:FN0418|3end_pyrimidine_regulatory_protein_PyrR_1059731..1059881_POSITIVE 188 142 106 39 1 NC_003454:FN0167|3end_hypothetical_protein_794416..794566_POSITIVE 188 120 75 70 27 NC_003454:FN0054|3end_tyrosyl-tRNA_synthetase_692136..692286_POSITIVE 187 155 113 75 18 NC_003454:FN0382|3end_hypothetical_protein_1014224..1014374_POSITIVE 184 86 43 118 65 NC_003454:FN1610|3end_33_kDa_chaperonin_119740..119890_NEGATIVE 181 109 65 46 5 NC_003454:FN2037|3end_LSU_ribosomal_protein_L12P_(L7/L12)_546769..546919_NEGATIVE 177 137 93 36 4 NC_003454:FN0217|3end_Catabolite_gene_activator_841735..841885_POSITIVE 176 153 120 108 65 NC_003454:FN1932|3end_Dephospho-CoA_kinase_432418..432568_NEGATIVE 173 90 44 146 102 NC_003454:FN0891|3end_DNAse_I_homologous_protein_DHP2_precursor_1552087..1552237_NEGATIVE 173 130 86 88 46 NC_003454:FN1529|3end_hypothetical_protein_42993..43143_NEGATIVE 172 155 116 38 1 NC_003454:FN1269|3end_Hypothetical_lipoprotein_1925613..1925763_NEGATIVE 171 150 112 68 32 NC_003454:FN0369|3end_Ribosomal-protein-alanine_acetyltransferase_998464..998614_NEGATIVE 170 103 63 62 17 NC_003454:FN0672|3end_ATPase_1329042..1329192_POSITIVE