Origin IGS: tcctacaacataattatcaatttctcttacacctaaatatgttattgatggaacaagcccttctggtacagtatcttcactacttttgatttttaattcttcaagccagccttcaacagatgaaaatttctctaaactggttgaactatttataatagctttttcaaataaagcttcttctttgtatttaataaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aactatttatcttccttgtacaattttttatattatacaaagataaatatgacatcatctgtcaagttatatatattttttagcaaactcatctattatatttttaaattgttttctaatcataagtctctataatatatactggcaaggggaataatactgttttttattaaatacaaagaagaagctttatttgaaaaagctattataaatagttcaaccagtttagagaaattttcatctgttgaaggctggcttgaagaattaaaaatcaaaagtagtgaagatactgtaccagaagggcttgttccatcaataacatatttaggtgtaagagaaattgataattatgttgtagga Mask Tandem Repeat Region ================================================ tcctacaacataattatcaatttctcttacacctaaatatgttattgatggaacaagcccttctggtacagtatcttcactacttttgatttttaattcttcaagccagccttcaacagatgaaaatttctctaaactggttgaactatttataatagctttttcaaataaagcttcttctttgtatttaataaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Find is-nt database================================================ Query_seq: FN0104:FN0105|FN0104:FN0105:Acetyltransferase:Hypothetical cytosolic protein:<-->:739987..740346 360 tcctacaacataattatcaatttctcttacacctaaatatgttattgatggaacaagcccttctggtacagtatcttcactacttttgatttttaattcttcaagccagccttcaacagatgaaaatttctctaaactggttgaactatttataatagctttttcaaataaagcttcttctttgtatttaataaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0104:FN0105|FN0104:FN0105:Acetyltransferase:Hypothetical cytosolic protein:<-->:739987..740346 360 tcctacaacataattatcaatttctcttacacctaaatatgttattgatggaacaagcccttctggtacagtatcttcactacttttgatttttaattcttcaagccagccttcaacagatgaaaatttctctaaactggttgaactatttataatagctttttcaaataaagcttcttctttgtatttaataaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0104:FN0105|FN0104:FN0105:Acetyltransferase:Hypothetical cytosolic protein:<-->:739987..740346 360 tcctacaacataattatcaatttctcttacacctaaatatgttattgatggaacaagcccttctggtacagtatcttcactacttttgatttttaattcttcaagccagccttcaacagatgaaaatttctctaaactggttgaactatttataatagctttttcaaataaagcttcttctttgtatttaataaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 1 Max: 192 Len: 192 Subject: UniRef90_Q7P7D9 Cluster: Acetyltransferase; n=2; Fusobacterium nucleatum|Rep: Acetyltransferase - Fusobacterium nucleatum subsp. vincentii ATCC 49256 HSP 1 e-value: 2.0E-17 bit: 90.1 Len: 192 Query Start:1 Query End:192 Subject Strand: null Subject Start: 18 Subject End: 81 I K Y K E E A L F E K A I I N S S T S L E K F S S V E G W L E E L K I K S S E D T V P E G L V P S I T Y L G V R E I D N Y V V G ........................................................................................................................................................................ L K Y K K E F L E A E P I I N G S A G L D R F S T I E E W L E E L K K R S S K D T V P E G L V P S S T Y L G I R E K D N Y I V G ........................................................................................................................................................................ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 20 End: 238 ................... M S L T P K Y V I D G T S P S G T V S S L L L I F N S S S Q P S T D E N F S K L V E L F I I A F S N K A S S L Y L I K N .......................................................................................................................... Protein_Len: 39 Strand: - Start: 229 End: 345 .................................................................................................................................................................................................................................... L S K H N S F L K F I Y Y I L K S F F I Y L K V S S T M N I K T Y Y L F N Y M ............... Protein_Len: 32 Strand: - Start: 207 End: 302 .............................................................................................................................................................................................................. E G Q W Y I N Y L S I I L F C N L F I I S S N A L F Y I Y S S M .......................................................... Protein_Len: 60 Strand: - Start: 1 End: 222 I S P V L G E P V T D E S S K I K L E E L W G E V S S F K E L S T S S N I I A K E F L A E E K Y K I F F L I I G R A L M .......................................................................................................................................... nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 127 End: 156 ..............................................................................................................................TTTCTCTAAACTGGTTGAACTATTTATAAT............................................................................................................................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 56 HP_score: -4.3 Tail_Score: -3.8077 Start: 297 End: 311 Full_Region: AAAATATATATAACT TGACA GATGA TGTCA TATTTATCTTTGTAT ........................................................................................................................................................................................................................................................................................................TGACAGATGATGTCA................................................. Find igs database================================================ Query_seq: FN0104:FN0105|FN0104:FN0105:Acetyltransferase:Hypothetical cytosolic protein:<-->:739987..740346 360 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Intra-Species Hit: Count: 1 Min: 193 Max: 360 Len: 168 Subject: NC_003454_FN0104_FN0105|Acetyltransferase:Hypothetical cytosolic protein|NEGATIVE:POSITIVE|[739987,740346]|360 HSP 1 e-value: 2.0E-90 bit: 333.0 Len: 168 Query Start:193 Query End:360 Subject Strand: POSITIVE Subject Start: 193 Subject End: 360 ................................................................................................................................................................................................aaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt ................................................................................................................................................................................................aaaaaacagtattattccccttgccagtatatattatagagacttatgattagaaaacaatttaaaaatataatagatgagtttgctaaaaaatatatataacttgacagatgatgtcatatttatctttgtataatataaaaaattgtacaaggaagataaatagtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAACAGUAUUAUUCCCCUUGCCAGUAUAUAUUAUAGAGACUUAUGAUUAGAAAACAAUUUAAAAAUAUAAUAGAUGAGUUUGCUAAAAAAUAUAUAUAACUUGACAGAUGAUGUCAUAUUUAUCUUUGUAUAAUAUAAAAAAUUGUACAAGGAAGAUAAAUAGUU ..........................(((((((((.(((((((((((.((((..................))))..)))))))).)))...)))))))))....(((((.....)))))((((((((((((((((((.......))))))))...))))))))))... (-29.17) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACUAUUUAUCUUCCUUGUACAAUUUUUUAUAUUAUACAAAGAUAAAUAUGACAUCAUCUGUCAAGUUAUAUAUAUUUUUUAGCAAACUCAUCUAUUAUAUUUUUAAAUUGUUUUCUAAUCAUAAGUCUCUAUAAUAUAUACUGGCAAGGGGAAUAAUACUGUUUUUU (((...((((..(((((((.........(((((((((...((((.(((.(((((.....))))).)))...........((((.((((......................)))).))))......)))).))))))))).....)))))))..))))....))).... (-24.59) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 208 90 41 62 3 NC_003454:FN1800|5end_Peptidyl-prolyl_cis-trans_isomerase_281630..281860_POSITIVE 207 149 108 94 54 NC_003454:FN0512|5end_Flavoprotein_1162597..1162827_NEGATIVE 207 50 1 80 25 NC_003454:FN1744|5end_Transporter_242920..243150_NEGATIVE 205 47 4 63 25 NC_003454:FN1025|5end_Guanine-hypoxanthine_permease_1679931..1680161_NEGATIVE 193 151 115 189 145 NC_003454:FN1972|5end_hypothetical_protein_472071..472301_NEGATIVE 192 133 103 160 126 NC_003454:FN0796|5end_pyruvate_phosphate_dikinase_1453008..1453238_POSITIVE 191 58 15 126 79 NC_003454:FN0689|5end_hypothetical_protein_1344658..1344888_POSITIVE 186 60 15 66 14 NC_003454:FN1523|5end_Dipeptide-binding_protein_28207..28437_POSITIVE 181 45 7 81 49 NC_003454:FN0751|5end_L-asparaginase_I_1410731..1410961_NEGATIVE 180 118 74 188 151 NC_003454:FN1222|5end_hypothetical_protein_1881969..1882199_NEGATIVE 178 38 8 83 45 NC_003454:FN1740|5end_ATP_synthase_subunit_K_238400..238630_NEGATIVE 177 27 8 110 84 NC_003454:FN0344|5end_Methyltransferase_977761..977991_NEGATIVE 175 60 19 212 176 NC_003454:FN0363|5end_ATP_synthase_C_chain,_sodium_ion_specific_993703..993933_NEGATIVE 173 160 112 128 83 NC_003454:FN1137|5end_Phosphonates_transport_system_permease_protein_phnE_1783769..1783999_POSITIVE 170 50 7 189 143 NC_003454:FN1630|5end_SSU_ribosomal_protein_S8P_134931..135161_NEGATIVE 169 150 115 122 86 NC_003454:FN0191|5end_ATP-dependent_DNA_helicase_recG_815752..815982_POSITIVE 168 127 83 218 164 NC_003454:FN1039|5end_Branched-chain_amino_acid_transport_protein_azlC_1690997..1691227_POSITIVE 164 45 5 119 87 NC_003454:FN0646|5end_Glutamyl-tRNA_reductase_1303289..1303519_NEGATIVE 163 38 15 193 164 NC_003454:FN0552|5end_Serine_racemase_1204715..1204945_NEGATIVE 160 91 57 207 177 NC_003454:FN0398|5end_Dipeptide_transport_system_permease_protein_dppC_1036758..1036988_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 205 47 4 40 2 NC_003454:FN1026|3end_tRNA_pseudouridine_synthase_A_1679954..1680104_NEGATIVE 199 80 37 48 9 NC_003454:FN0436|3end_Urea_carboxylase_1077851..1078001_NEGATIVE 198 50 7 47 1 NC_003454:FN1745|3end_Cystathionine_gamma-synthase_242953..243103_NEGATIVE 192 133 103 148 114 NC_003454:FN0795|3end_CBS_domain_containing_protein_1453076..1453226_POSITIVE 185 37 6 61 22 NC_003454:FN0173|3end_hypothetical_protein_799304..799454_POSITIVE 181 45 7 91 59 NC_003454:FN0752|3end_Proline_iminopeptidase_1410721..1410871_NEGATIVE 177 27 8 110 84 NC_003454:FN0345|3end_Permease_977761..977911_NEGATIVE 168 133 101 139 106 NC_003454:FN1136|3end_Phosphonates_transport_ATP-binding_protein_phnC_1783880..1784030_POSITIVE 168 29 7 136 114 NC_003454:FN1631|3end_SSU_ribosomal_protein_S14P_134960..135110_NEGATIVE 164 38 6 76 43 NC_003454:FN0793|3end_Sodium/glutamate_symport_carrier_protein_1451791..1451941_POSITIVE 164 42 8 143 97 NC_003454:FN0364|3end_ATP_synthase_A_chain,_sodium_ion_specific_993757..993907_NEGATIVE 158 50 9 78 22 NC_003454:FN1337|3end_hypothetical_protein_1990288..1990438_NEGATIVE 156 44 8 31 1 NC_003454:FN0335|3end_Outer_membrane_porin_F_969157..969307_NEGATIVE 156 117 78 45 4 NC_003454:FN0250|3end_hypothetical_protein_873815..873965_POSITIVE 156 117 78 45 4 NC_003454:FN2062|3end_hypothetical_protein_574107..574257_NEGATIVE 156 117 78 45 4 NC_003454:FN2051|3end_hypothetical_protein_560031..560181_NEGATIVE 155 114 77 87 48 NC_003454:FN0451|3end_hypothetical_protein_1095427..1095577_POSITIVE 154 118 77 144 106 NC_003454:FN0859|3end_hypothetical_protein_1520720..1520870_NEGATIVE 154 49 18 51 17 NC_003454:FN2086|3end_General_secretion_pathway_protein_D_597507..597657_NEGATIVE 152 160 135 67 37 NC_003454:FN0981|3end_phosphoribosylamine--glycine_ligase_1632840..1632990_NEGATIVE