Origin IGS: aactaaaaaatagttcgttactagccaaatttcttaacggataaaaattaagaattcgctgtaattttggcatctgtaagaagctctaaatgaacaagttcattaaaagcttctaagattgctgacaagtcagcttcaaacacaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tactcttcctccatcttaccttattttattagtaaaaaacttacaaaaataagtgagttacattccagattttaggataaaaattaaatagaatgagccgattaattttggtgtgtttgaagctgacttgtcagcaatcttagaagcttttaatgaacttgttcatttagagcttcttacagatgccaaaattacagcgaattcttaatttttatccgttaagaaatttggctagtaacgaactattttttagtt Mask Tandem Repeat Region ================================================ aactaaaaaatagttcgttactagccaaatttcttaacggataaaaattaagaattcgctgtaattttggcatctgtaagaagctctaaatgaacaagttcattaaaagcttctaagattgctgacaagtcagcttcaaacacaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Find is-nt database================================================ Query_seq: FN0084:FN0085|FN0084:FN0085:Acetaldehyde dehydrogenase [acetylating]:Ethanolamine utilization cobalamin adenosyltransferase:->->:723155..723409 255 aactaaaaaatagttcgttactagccaaatttcttaacggataaaaattaagaattcgctgtaattttggcatctgtaagaagctctaaatgaacaagttcattaaaagcttctaagattgctgacaagtcagcttcaaacacaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0084:FN0085|FN0084:FN0085:Acetaldehyde dehydrogenase [acetylating]:Ethanolamine utilization cobalamin adenosyltransferase:->->:723155..723409 255 aactaaaaaatagttcgttactagccaaatttcttaacggataaaaattaagaattcgctgtaattttggcatctgtaagaagctctaaatgaacaagttcattaaaagcttctaagattgctgacaagtcagcttcaaacacaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0084:FN0085|FN0084:FN0085:Acetaldehyde dehydrogenase [acetylating]:Ethanolamine utilization cobalamin adenosyltransferase:->->:723155..723409 255 aactaaaaaatagttcgttactagccaaatttcttaacggataaaaattaagaattcgctgtaattttggcatctgtaagaagctctaaatgaacaagttcattaaaagcttctaagattgctgacaagtcagcttcaaacacaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 5 Max: 143 Len: 139 Subject: UniRef90_Q7P6T5 Cluster: Hypothetical protein FNV1606; n=1; Fusobacterium nucleatum subsp. vincentii ATCC 49256|Rep: Hypothetical protein FNV1606 - Fusobacterium nucleatum subsp. vincentii ATCC 49256 HSP 1 e-value: 9.0E-8 bit: 48.9 Len: 111 Query Start:33 Query End:143 Subject Strand: null Subject Start: 1 Subject End: 37 ................................ V F E A D L S A I L E A F N E L V H L E L L T D A K I T A N S * F L S V K ................................................................................................................ ................................ M F E A N L L A I L E A I N E L V H L E L L T D A E F A A N V N F S S L R ................................................................................................................ HSP 2 e-value: 9.0E-8 bit: 29.3 Len: 48 Query Start:5 Query End:52 Subject Strand: null Subject Start: 31 Subject End: 46 .... L N F Y P L R N L A S N E L F F ........................................................................................................................................................................................................... .... V N F S S L R N L A S N E L F F ........................................................................................................................................................................................................... aactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: - Start: 51 End: 155 .................................................. S N A T I K A D T L L E L H V L E N F A E L I A S L D A E F V G F N M .................................................................................................... aactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: FN0084:FN0085|FN0084:FN0085:Acetaldehyde dehydrogenase [acetylating]:Ethanolamine utilization cobalamin adenosyltransferase:->->:723155..723409 255 aactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnaccaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Intra-Species Hit: Count: 1 Min: 144 Max: 255 Len: 112 Subject: NC_003454_FN0084_FN0085|Acetaldehyde dehydrogenase [acetylating]:Ethanolamine utilization cobalamin adenosyltransferase|POSITIVE:POSITIVE|[723155, 723409]|255 HSP 1 e-value: 5.0E-57 bit: 222.0 Len: 112 Query Start:144 Query End:255 Subject Strand: POSITIVE Subject Start: 144 Subject End: 255 ...............................................................................................................................................accaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta ...............................................................................................................................................accaaaattaatcggctcattctatttaatttttatcctaaaatctggaatgtaactcacttatttttgtaagttttttactaataaaataaggtaagatggaggaagagta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCAAAAUUAAUCGGCUCAUUCUAUUUAAUUUUUAUCCUAAAAUCUGGAAUGUAACUCACUUAUUUUUGUAAGUUUUUUACUAAUAAAAUAAGGUAAGAUGGAGGAAGAGUA ..............((((.((((((((.(((((..(((........)))..((((...((((((....))))))...)))).........))))).))))))))...)))). (-19.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UACUCUUCCUCCAUCUUACCUUAUUUUAUUAGUAAAAAACUUACAAAAAUAAGUGAGUUACAUUCCAGAUUUUAGGAUAAAAAUUAAAUAGAAUGAGCCGAUUAAUUUUGGU ...................(((((((((((.......(((((((........)))))))....(((........))).........)))))))))))((((......)))). (-18.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 729 60 12 131 83 NC_003454:FN1460|5end_Hypothetical_cytosolic_protein_2141429..2141659_POSITIVE 377 67 21 228 185 NC_003454:FN1615|5end_Integral_membrane_protein_125125..125355_POSITIVE 360 68 28 193 153 NC_003454:FN0367|5end_Integral_membrane_protein_996901..997131_NEGATIVE 342 60 12 168 119 NC_003454:FN1604|5end_hypothetical_protein_113822..114052_NEGATIVE 331 60 12 166 117 NC_003454:FN1507|5end_riboflavin_synthase_subunit_alpha_12073..12303_POSITIVE 324 70 45 158 133 NC_003454:FN1898|5end_Sugar_transport_ATP-binding_protein_386569..386799_NEGATIVE 308 67 29 159 125 NC_003454:FN1440|5end_1-phosphofructokinase_2107238..2107468_POSITIVE 225 67 29 94 44 NC_003454:FN0424|5end_Dihydroorotate_dehydrogenase_1067119..1067349_POSITIVE 203 96 63 214 180 NC_003454:FN1338|5end_hypothetical_protein_1991763..1991993_NEGATIVE 203 96 63 214 180 NC_003454:FN0530|5end_hypothetical_protein_1186275..1186505_POSITIVE 203 96 63 214 180 NC_003454:FN0404|5end_hypothetical_protein_1042213..1042443_POSITIVE 203 96 63 214 180 NC_003454:FN0373|5end_hypothetical_protein_1002385..1002615_POSITIVE 203 96 63 214 180 NC_003454:FN1510|5end_hypothetical_protein_14311..14541_NEGATIVE 200 104 63 220 184 NC_003454:FN1187|5end_Amino_acid-binding_protein_1849882..1850112_POSITIVE 194 83 46 226 184 NC_003454:FN1241|5end_Hypohetical_Teichoic_Acid_Biosynthesis_Protein_1898512..1898742_NEGATIVE 193 68 21 86 36 NC_003454:FN2078|5end_Transcriptional_regulator,_DeoR_family_590266..590496_NEGATIVE 189 67 21 153 106 NC_003454:FN1578|5end_hypothetical_protein_88796..89026_NEGATIVE 186 48 8 218 182 NC_003454:FN0611|5end_Threonyl-tRNA_synthetase_1268359..1268589_POSITIVE 185 109 78 175 150 NC_003454:FN0797|5end_hypothetical_protein_1455799..1456029_NEGATIVE 182 105 61 138 88 NC_003454:FN1474|5end_N-acetylmannosamine_kinase_2156888..2157118_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 655 58 14 66 22 NC_003454:FN0713|3end_Chromate_transport_protein_1371943..1372093_POSITIVE 392 60 13 49 1 NC_003454:FN0796|3end_pyruvate_phosphate_dikinase_1455643..1455793_POSITIVE 360 68 28 56 16 NC_003454:FN0368|3end_adenylosuccinate_lyase_997038..997188_NEGATIVE 334 68 30 40 1 NC_003454:FN1652|3end_Oligopeptide-binding_protein_oppA_146041..146191_NEGATIVE 331 60 12 73 24 NC_003454:FN1506|3end_5-amino-6-(5-phosphoribosylamino)uracil_reductase_12060..12210_POSITIVE 308 67 29 86 52 NC_003454:FN1439|3end_Transcriptional_regulator,_DeoR_family_2107245..2107395_POSITIVE 262 56 13 71 27 NC_003454:FN1836|3end_Tetratricopeptide_repeat_family_protein_323904..324054_NEGATIVE 238 48 2 66 21 NC_003454:FN0084|3end_Acetaldehyde_dehydrogenase_[acetylating]_723094..723244_POSITIVE 236 55 13 72 29 NC_003454:FN0483|3end_Uracil_phosphoribosyltransferase_1129301..1129451_POSITIVE 225 67 29 82 32 NC_003454:FN0423|3end_Dihydroorotate_dehydrogenase_electron_transfer_subunit_1067187..1067337_POSITIVE 220 84 43 43 9 NC_003454:FN1605|3end_Adenylosuccinate_synthetase_113999..114149_NEGATIVE 201 70 21 50 2 NC_003454:FN0116|3end_Chaperone_protein_dnaK_750156..750306_POSITIVE 197 48 12 147 116 NC_003454:FN1265|3end_Outer_membrane_protein_1922019..1922169_POSITIVE 193 69 31 124 89 NC_003454:FN1545|3end_Ferredoxin_like_protein_59497..59647_POSITIVE 187 56 13 81 37 NC_003454:FN0391|3end_Hydrolase_(HAD_superfamily)_1029496..1029646_POSITIVE 186 48 8 141 105 NC_003454:FN0610|3end_hypothetical_protein_1268362..1268512_POSITIVE 185 86 51 112 75 NC_003454:FN0109|3end_hypothetical_protein_744333..744483_NEGATIVE 182 105 61 135 85 NC_003454:FN1473|3end_N-acetylneuraminate_transporter_large_subunit_2156965..2157115_POSITIVE 182 56 13 71 27 NC_003454:FN1461|3end_Histidinol-phosphatase_2141603..2141753_NEGATIVE 181 57 11 51 10 NC_003454:FN0709|3end_Virulence_factor_mviN_1368888..1369038_POSITIVE