Origin IGS: aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctaatcctccattctacctctctaccaccttaaatattacacttattttgaataatggattcccttttattcaaaataagggtgacagtcgtcaccctatgaaatt Mask Tandem Repeat Region ================================================ aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Find is-nt database================================================ Query_seq: FN0083:FN0084|FN0083:FN0084:Ethanolamine utilization protein eutM precursor:Acetaldehyde dehydrogenase [acetylating]:->->:721605..721711 107 aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0083:FN0084|FN0083:FN0084:Ethanolamine utilization protein eutM precursor:Acetaldehyde dehydrogenase [acetylating]:->->:721605..721711 107 aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0083:FN0084|FN0083:FN0084:Ethanolamine utilization protein eutM precursor:Acetaldehyde dehydrogenase [acetylating]:->->:721605..721711 107 aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Predict ORF larger than 30AA ================================================ aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 71 HP_score: -8.5 Tail_Score: -2.97289 Start: 27 End: 65 Full_Region: GTGACGACTGTCACC CTTATTTTGAATAAAAGG GAAT CCATTATTCAAAATAAG TGTAATATTTAAGGT ..........................CTTATTTTGAATAAAAGGGAATCCATTATTCAAAATAAG.......................................... Find igs database================================================ Query_seq: FN0083:FN0084|FN0083:FN0084:Ethanolamine utilization protein eutM precursor:Acetaldehyde dehydrogenase [acetylating]:->->:721605..721711 107 aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Intra-Species Hit: Count: 1 Min: 1 Max: 107 Len: 107 Subject: NC_003454_FN0083_FN0084|Ethanolamine utilization protein eutM precursor:Acetaldehyde dehydrogenase [acetylating]|POSITIVE:POSITIVE|[721605,721711]|107 HSP 1 e-value: 2.0E-54 bit: 212.0 Len: 107 Query Start:1 Query End:107 Subject Strand: POSITIVE Subject Start: 1 Subject End: 107 aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga aatttcatagggtgacgactgtcacccttattttgaataaaagggaatccattattcaaaataagtgtaatatttaaggtggtagagaggtagaatggaggattaga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUCAUAGGGUGACGACUGUCACCCUUAUUUUGAAUAAAAGGGAAUCCAUUAUUCAAAAUAAGUGUAAUAUUUAAGGUGGUAGAGAGGUAGAAUGGAGGAUUAGA ..((((((..(....)..((..((((((((((((((((((..((....)).))))))))))))))............))))..)).........))))))....... (-25.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUAAUCCUCCAUUCUACCUCUCUACCACCUUAAAUAUUACACUUAUUUUGAAUAAUGGAUUCCCUUUUAUUCAAAAUAAGGGUGACAGUCGUCACCCUAUGAAAUU ............(((.............................((((((((((((.((....))..))))))))))))((((((((....))))))))..)))... (-24.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 214 98 58 218 177 NC_003454:FN0729|5end_Phosphoglycerate_mutase_1386354..1386584_POSITIVE 207 85 42 210 155 NC_003454:FN1882|5end_hypothetical_protein_368560..368790_POSITIVE 194 88 41 119 67 NC_003454:FN0534|5end_hypothetical_protein_1188858..1189088_POSITIVE 188 44 1 109 59 NC_003454:FN1292|5end_hypothetical_protein_1950804..1951034_NEGATIVE 186 99 68 58 28 NC_003454:FN1904|5end_Transcriptional_regulator,_MarR_family_393107..393337_POSITIVE 184 99 65 200 157 NC_003454:FN1435|5end_Export_ABC_transporter_2105034..2105264_POSITIVE 184 99 55 50 5 NC_003454:FN0166|5end_hypothetical_protein_793732..793962_POSITIVE 181 99 75 222 201 NC_003454:FN1478|5end_hypothetical_protein_2160663..2160893_NEGATIVE 176 40 9 155 127 NC_003454:FN1622|5end_Uridylate_kinase_129640..129870_POSITIVE 175 47 4 53 1 NC_003454:FN1353|5end_ABC_transporter_permease_protein_2005386..2005616_NEGATIVE 174 99 60 174 137 NC_003454:FN0842|5end_hypothetical_protein_1503055..1503285_NEGATIVE 174 88 48 206 162 NC_003454:FN0686|5end_Integral_membrane_protein_1342498..1342728_NEGATIVE 173 43 7 33 3 NC_003454:FN1170|5end_Pyruvate-flavodoxin_oxidoreductase_1832273..1832503_NEGATIVE 172 47 23 110 82 NC_003454:FN1228|5end_hypothetical_protein_1886190..1886420_NEGATIVE 171 93 63 195 160 NC_003454:FN0407|5end_hypothetical_protein_1045178..1045408_NEGATIVE 170 53 20 37 1 NC_003454:FN1118|5end_hypothetical_protein_1760559..1760789_POSITIVE 169 44 10 225 173 NC_003454:FN1053|5end_hypothetical_protein_1701023..1701253_POSITIVE 169 58 11 72 23 NC_003454:FN0443|5end_hypothetical_protein_1082680..1082910_POSITIVE 167 100 58 219 163 NC_003454:FN1459|5end_Phospho-N-acetylmuramoyl-pentapeptide-_transferase_2141360..2141590_NEGATIVE 167 35 6 117 89 NC_003454:FN0706|5end_Hypothetical_cytosolic_protein_1364823..1365053_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 68 24 67 23 NC_003454:FN0083|3end_Ethanolamine_utilization_protein_eutM_precursor_721544..721694_POSITIVE 215 100 54 73 29 NC_003454:FN2109|3end_UDP-glucose_4-epimerase_621917..622067_POSITIVE 199 67 24 72 33 NC_003454:FN1667|3end_dTDP-glucose_4,6-dehydratase_163273..163423_NEGATIVE 176 40 9 90 62 NC_003454:FN1621|3end_elongation_factor_Ts_129655..129805_POSITIVE 174 99 60 43 6 NC_003454:FN0843|3end_hypothetical_protein_1503186..1503336_NEGATIVE 172 47 23 40 12 NC_003454:FN1229|3end_hypothetical_protein_1886260..1886410_NEGATIVE 170 33 9 67 39 NC_003454:FN0793|3end_Sodium/glutamate_symport_carrier_protein_1451791..1451941_POSITIVE 167 35 6 98 70 NC_003454:FN0705|3end_DNA_polymerase_I_1364884..1365034_POSITIVE 166 80 35 101 52 NC_003454:FN0100|3end_Flavodoxins/hemoproteins_735119..735269_NEGATIVE 164 58 20 130 99 NC_003454:FN1679|3end_LPS_biosynthesis_protein_WbpG_174590..174740_NEGATIVE 163 100 78 37 16 NC_003454:FN0683|3end_hypothetical_protein_1338514..1338664_NEGATIVE 162 42 9 134 97 NC_003454:FN2057|3end_hypothetical_protein_565286..565436_NEGATIVE 161 47 8 43 4 NC_003454:FN1354|3end_ABC_transporter_permease_protein_2005396..2005546_NEGATIVE 160 39 9 75 45 NC_003454:FN0954|3end_Hypothetical_cytosolic_protein_1610369..1610519_NEGATIVE 159 51 17 47 14 NC_003454:FN0559|3end_Phosphoglucomutase_1213138..1213288_POSITIVE 159 100 56 150 108 NC_003454:FN1743|3end_Multidrug-efflux_transporter_2_regulator_241032..241182_NEGATIVE 158 95 72 136 111 NC_003454:FN1754|3end_thiazole_synthase_250344..250494_NEGATIVE 156 45 8 43 9 NC_003454:FN1784|3end_hypothetical_protein_265735..265885_NEGATIVE 152 99 63 36 1 NC_003454:FN0165|3end_hypothetical_protein_793798..793948_POSITIVE 151 85 42 107 62 NC_003454:FN1356|3end_hypothetical_protein_2008160..2008310_POSITIVE