Origin IGS: ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacaactccttatttaaattaactaatagtaaaaaaagctgtgaacagaactatcctgtccatcagcttcattgctttctatttttataatatactccattgtatatcttat Mask Tandem Repeat Region ================================================ ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Find is-nt database================================================ Query_seq: FN0077:FN0078|FN0077:FN0078:Ethanolamine two-component sensor kinase:Ethanolamine utilization protein eutA:->->:716233..716344 112 ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0077:FN0078|FN0077:FN0078:Ethanolamine two-component sensor kinase:Ethanolamine utilization protein eutA:->->:716233..716344 112 ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0077:FN0078|FN0077:FN0078:Ethanolamine two-component sensor kinase:Ethanolamine utilization protein eutA:->->:716233..716344 112 ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Predict ORF larger than 30AA ================================================ ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -8.7 Tail_Score: -5.63374 Start: 46 End: 75 Full_Region: ATAGAAAGCAATGAA GCTGATGGACAGGA TAG TTCTGTTCACAGC TTTTTTTACTATTAG .............................................GCTGATGGACAGGATAGTTCTGTTCACAGC..................................... Find igs database================================================ Query_seq: FN0077:FN0078|FN0077:FN0078:Ethanolamine two-component sensor kinase:Ethanolamine utilization protein eutA:->->:716233..716344 112 ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: NC_003454_FN0077_FN0078|Ethanolamine two-component sensor kinase:Ethanolamine utilization protein eutA|POSITIVE:POSITIVE|[716233,716344]|112 HSP 1 e-value: 6.0E-45 bit: 180.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagcnnnnnnnactattagttaatttaaataaggagttgtt ataagatatacaatggagtatattataaaaatagaaagcaatgaagctgatggacaggatagttctgttcacagctttttttactattagttaatttaaataaggagttgtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAGAUAUACAAUGGAGUAUAUUAUAAAAAUAGAAAGCAAUGAAGCUGAUGGACAGGAUAGUUCUGUUCACAGCUUUUUUUACUAUUAGUUAAUUUAAAUAAGGAGUUGUU ....(((((((......))))))).....(((((((((....(((((((.(((((((((...)))))))))))))))))))).)))))...((((((.......)))))).. (-25.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACAACUCCUUAUUUAAAUUAACUAAUAGUAAAAAAAGCUGUGAACAGAACUAUCCUGUCCAUCAGCUUCAUUGCUUUCUAUUUUUAUAAUAUACUCCAUUGUAUAUCUUAU ...........................(((((...((((((((.((((.......)))).)).))))))..))))).........((..((((((......))))))..)). (-18.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 273 80 33 168 122 NC_003454:FN1751|5end_Pyruvate-flavodoxin_oxidoreductase_248067..248297_POSITIVE 236 85 41 92 37 NC_003454:FN0128|5end_Spermidine/putrescine-binding_protein_756651..756881_POSITIVE 226 88 48 175 137 NC_003454:FN0104|5end_Acetyltransferase_739896..740126_NEGATIVE 211 90 41 171 117 NC_003454:FN0740|5end_imidazolonepropionase_1398914..1399144_NEGATIVE 211 84 41 174 130 NC_003454:FN1842|5end_Dihydroxyacetone_kinase_phosphotransfer_protein_331491..331721_POSITIVE 208 87 45 188 142 NC_003454:FN1702|5end_undecaprenyl_pyrophosphate_phosphatase_197964..198194_NEGATIVE 208 109 62 231 172 NC_003454:FN1506|5end_5-amino-6-(5-phosphoribosylamino)uracil_reductase_10871..11101_POSITIVE 205 74 33 60 19 NC_003454:FN1805|5end_hypothetical_protein_286453..286683_POSITIVE 202 54 12 155 117 NC_003454:FN0590|5end_N-acyl-L-amino_acid_amidohydrolase_1244037..1244267_NEGATIVE 200 77 38 59 18 NC_003454:FN1457|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-_Pentapeptide_pyrophosphoryl-undecaprenol_N-acetylglucosamine_transferase_2138976..2139206_NEGATIVE 199 77 38 50 24 NC_003454:FN1723|5end_glucose-inhibited_division_protein_A_222645..222875_NEGATIVE 197 88 47 95 46 NC_003454:FN0151|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_778771..779001_POSITIVE 196 71 31 63 29 NC_003454:FN1421|5end_Pyruvate-flavodoxin_oxidoreductase_2083305..2083535_POSITIVE 193 86 45 149 105 NC_003454:FN0743|5end_ATP-dependent_helicase,_DinG_family_1400850..1401080_POSITIVE 191 76 33 127 82 NC_003454:FN0764|5end_Transporter,_LysE_family_1420327..1420557_POSITIVE 190 86 54 215 188 NC_003454:FN1215|5end_Hypothetical_cytosolic_protein_1876167..1876397_NEGATIVE 188 57 17 169 114 NC_003454:FN1416|5end_AMINOTRANSFERASE_CLASS-I_2076893..2077123_POSITIVE 188 70 21 63 14 NC_003454:FN1335|5end_Protein_translocase_subunit_YajC_1989011..1989241_NEGATIVE 188 79 32 115 74 NC_003454:FN1749|5end_Pyruvate-flavodoxin_oxidoreductase_247461..247691_POSITIVE 187 87 44 91 37 NC_003454:FN1070|5end_glucose-inhibited_division_protein_A_1717121..1717351_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 77 32 47 8 NC_003454:FN0077|3end_Ethanolamine_two-component_sensor_kinase_716172..716322_POSITIVE 273 80 33 100 54 NC_003454:FN1750|3end_Pyruvate-flavodoxin_oxidoreductase_248079..248229_POSITIVE 211 90 41 94 40 NC_003454:FN0741|3end_Glutamate_formiminotransferase_1398991..1399141_NEGATIVE 202 54 12 139 101 NC_003454:FN0591|3end_Putative_efflux_pump_component_MtrF_1244053..1244203_NEGATIVE 201 88 44 138 93 NC_003454:FN1339|3end_Transposase_1993230..1993380_POSITIVE 201 88 44 138 93 NC_003454:FN0529|3end_Transposase_1184888..1185038_NEGATIVE 201 88 44 138 93 NC_003454:FN0403|3end_Transposase_1040826..1040976_NEGATIVE 201 88 44 138 93 NC_003454:FN0372|3end_Transposase_1000998..1001148_NEGATIVE 201 88 44 138 93 NC_003454:FN1511|3end_Transposase_15778..15928_POSITIVE 200 77 38 58 17 NC_003454:FN1458|3end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_2138977..2139127_NEGATIVE 199 77 38 41 15 NC_003454:FN1724|3end_Potassium_uptake_protein_KtrA_222654..222804_NEGATIVE 188 109 63 128 83 NC_003454:FN0564|3end_hypothetical_protein_1216466..1216616_NEGATIVE 188 79 32 133 92 NC_003454:FN1748|3end_Pyruvate-flavodoxin_oxidoreductase_247559..247709_POSITIVE 187 62 31 77 39 NC_003454:FN1602|3end_Hypothetical_cytosolic_protein_111022..111172_NEGATIVE 184 87 47 52 13 NC_003454:FN1117|3end_LSU_ribosomal_protein_L21P_1760636..1760786_POSITIVE 179 44 12 149 119 NC_003454:FN0340|3end_Hypothetical_membrane-spanning_Protein_972638..972788_NEGATIVE 179 74 37 70 36 NC_003454:FN0246|3end_hypothetical_protein_870633..870783_NEGATIVE 178 89 42 87 46 NC_003454:FN0446|3end_hypothetical_protein_1089404..1089554_POSITIVE 177 82 44 134 96 NC_003454:FN1780|3end_hypothetical_protein_260921..261071_NEGATIVE 176 72 33 63 21 NC_003454:FN0200|3end_Biotin_carboxyl_carrier_protein_of_glutaconyl-COA_decarboxylase_826607..826757_POSITIVE