Origin IGS: gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttccatatatctacctccatttattttcttataacttaaaataactctttttcttagttatttcattttctaaatggatagatatacttctatgtaaaataaaaaagactattgaaaattttagtcttttttaacttaaaatcttaatagtctttaatattttctagtttgagc Mask Tandem Repeat Region ================================================ gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Find is-nt database================================================ Query_seq: FN0063:FN0064|FN0063:FN0064:hypothetical protein:Hypothetical cytosolic protein:->->:699455..699628 174 gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: FN0063:FN0064|FN0063:FN0064:hypothetical protein:Hypothetical cytosolic protein:->->:699455..699628 174 gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: FN0063:FN0064|FN0063:FN0064:hypothetical protein:Hypothetical cytosolic protein:->->:699455..699628 174 gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Predict ORF larger than 30AA ================================================ gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 64 HP_score: -5.6 Tail_Score: -4.18494 Start: 42 End: 75 Full_Region: ATTAAGATTTTAAGT TAAAAAAGACTA AAATTTTCAA TAGTCTTTTTTA TTTTACATAGAAGTA .........................................TAAAAAAGACTAAAATTTTCAATAGTCTTTTTTA................................................................................................... Find igs database================================================ Query_seq: FN0063:FN0064|FN0063:FN0064:hypothetical protein:Hypothetical cytosolic protein:->->:699455..699628 174 gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Intra-Species Hit: Count: 1 Min: 1 Max: 174 Len: 174 Subject: NC_003454_FN0063_FN0064|hypothetical protein:Hypothetical cytosolic protein|POSITIVE:POSITIVE|[699455,699628]|174 HSP 1 e-value: 3.0E-94 bit: 345.0 Len: 174 Query Start:1 Query End:174 Subject Strand: POSITIVE Subject Start: 1 Subject End: 174 gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa gctcaaactagaaaatattaaagactattaagattttaagttaaaaaagactaaaattttcaatagtcttttttattttacatagaagtatatctatccatttagaaaatgaaataactaagaaaaagagttattttaagttataagaaaataaatggaggtagatatatggaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCUCAAACUAGAAAAUAUUAAAGACUAUUAAGAUUUUAAGUUAAAAAAGACUAAAAUUUUCAAUAGUCUUUUUUAUUUUACAUAGAAGUAUAUCUAUCCAUUUAGAAAAUGAAAUAACUAAGAAAAAGAGUUAUUUUAAGUUAUAAGAAAAUAAAUGGAGGUAGAUAUAUGGAA .......(((.((((((..(((((((((((((((((((.(((......)))))))))))).))))))))))..))))))...)))..(((((((((((((((((.((..((((((((((.........))))))))))..)).........))))))))..))))))))).... (-44.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUCCAUAUAUCUACCUCCAUUUAUUUUCUUAUAACUUAAAAUAACUCUUUUUCUUAGUUAUUUCAUUUUCUAAAUGGAUAGAUAUACUUCUAUGUAAAAUAAAAAAGACUAUUGAAAAUUUUAGUCUUUUUUAACUUAAAAUCUUAAUAGUCUUUAAUAUUUUCUAGUUUGAGC .....((((((((..((((((((.......((((((.((((.......))))...)))))).........))))))))))))))))...(((.(.((((((..((((((((((((..((((((((........)).)))))).))))))))))))..))))))))))....... (-32.39) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 103 67 81 44 NC_003454:FN2015|5end_ATP-dependent_protease_ATP-binding_subunit_518255..518485_NEGATIVE 197 106 64 69 22 NC_003454:FN0188|5end_bifunctional_methionine_sulfoxide_reductase_A/B_protein_812276..812506_POSITIVE 195 92 53 149 115 NC_003454:FN1228|5end_hypothetical_protein_1886190..1886420_NEGATIVE 194 102 66 92 48 NC_003454:FN0132|5end_Hemolysin_761715..761945_POSITIVE 186 79 51 162 132 NC_003454:FN0270|5end_GTP-binding_protein_era_896770..897000_POSITIVE 185 76 56 28 8 NC_003454:FN0925|5end_hypothetical_protein_1579921..1580151_POSITIVE 184 107 63 78 26 NC_003454:FN0900|5end_Metal_dependent_hydrolase_1559180..1559410_POSITIVE 181 34 1 149 116 NC_003454:FN2041|5end_Transcription_antitermination_protein_nusG_549659..549889_NEGATIVE 178 131 93 79 33 NC_003454:FN1251|5end_High-affinity_iron_permease_1908008..1908238_POSITIVE 178 103 61 93 52 NC_003454:FN0329|5end_50S_ribosomal_protein_L13_964133..964363_POSITIVE 175 72 42 138 99 NC_003454:FN0626|5end_Hypothetical_cytosolic_protein_1286092..1286322_NEGATIVE 173 97 57 168 132 NC_003454:FN0242|5end_Trk_system_potassium_uptake_protein_trkA_865228..865458_POSITIVE 171 93 57 84 42 NC_003454:FN1404|5end_Imidazolonepropionase_2063066..2063296_POSITIVE 171 101 61 91 49 NC_003454:FN0915|5end_PTS_system,_N-acetylglucosamine-specific_IIA_component_1572037..1572267_NEGATIVE 170 117 81 51 10 NC_003454:FN1584|5end_Beta-lactamase_96158..96388_NEGATIVE 167 104 67 143 101 NC_003454:FN0674|5end_Transporter_1329473..1329703_POSITIVE 166 123 81 80 46 NC_003454:FN1369|5end_Zinc_finger_protein_2019006..2019236_NEGATIVE 165 109 63 65 23 NC_003454:FN1461|5end_Histidinol-phosphatase_2143444..2143674_NEGATIVE 165 43 16 188 163 NC_003454:FN1315|5end_hypothetical_protein_1969454..1969684_NEGATIVE 165 103 62 106 64 NC_003454:FN0254|5end_Fusobacterium_outer_membrane_protein_family_876277..876507_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 247 103 67 69 32 NC_003454:FN2016|3end_ATP-dependent_Clp_protease_proteolytic_subunit_518267..518417_NEGATIVE 213 111 71 125 84 NC_003454:FN0793|3end_Sodium/glutamate_symport_carrier_protein_1451791..1451941_POSITIVE 195 92 53 79 45 NC_003454:FN1229|3end_hypothetical_protein_1886260..1886410_NEGATIVE 189 105 61 122 78 NC_003454:FN0029|3end_Flavodoxin_666596..666746_NEGATIVE 185 70 21 70 21 NC_003454:FN0063|3end_hypothetical_protein_699394..699544_POSITIVE 184 103 67 146 113 NC_003454:FN1010|3end_Hypothetical_cytosolic_protein_1664312..1664462_NEGATIVE 184 107 63 71 19 NC_003454:FN0899|3end_3-oxoacyl-[acyl-carrier_protein]_reductase_1559253..1559403_POSITIVE 184 106 61 117 81 NC_003454:FN2110|3end_Hypothetical_exported_24-amino_acid_repeat_protein_621908..622058_NEGATIVE 175 72 42 75 36 NC_003454:FN0627|3end_Glucosamine--fructose-6-phosphate_aminotransferase_[isomerizing]_1286155..1286305_NEGATIVE 171 93 57 71 29 NC_003454:FN1403|3end_Histidine_permease_2063133..2063283_POSITIVE 171 101 61 63 21 NC_003454:FN0916|3end_hypothetical_protein_1572065..1572215_NEGATIVE 171 167 137 42 15 NC_003454:FN0841|3end_Transposase_1502804..1502954_POSITIVE 168 170 122 50 2 NC_003454:FN0616|3end_hypothetical_protein_1275024..1275174_NEGATIVE 168 33 1 151 119 NC_003454:FN2042|3end_Protein_translocase_subunit_SecE_549656..549806_NEGATIVE 167 110 61 124 65 NC_003454:FN1385|3end_hypothetical_protein_2038394..2038544_NEGATIVE 167 104 67 118 76 NC_003454:FN0673|3end_EBSC_protein_1329528..1329678_POSITIVE 166 123 81 96 62 NC_003454:FN1370|3end_Endonuclease_2018990..2019140_NEGATIVE 165 43 16 142 117 NC_003454:FN1316|3end_NGG1-interacting_factor_3_1969500..1969650_NEGATIVE 163 104 62 130 80 NC_003454:FN0666|3end_hypothetical_protein_1322296..1322446_POSITIVE 160 132 94 51 14 NC_003454:FN0620|3end_hypothetical_protein_1279819..1279969_POSITIVE